http://2014hs.igem.org/wiki/index.php?title=Team:Lethbridge_Canada/parts&feed=atom&action=historyTeam:Lethbridge Canada/parts - Revision history2024-03-28T16:48:56ZRevision history for this page on the wikiMediaWiki 1.16.5http://2014hs.igem.org/wiki/index.php?title=Team:Lethbridge_Canada/parts&diff=28068&oldid=prevT Dang at 21:59, 2 July 20142014-07-02T21:59:42Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p class="ContentParagraph">K331007 relates specifically to our project as it is the signal sequence already present in beta-lactamase. K331009 also relates as it will export our other antibiotic degrading enzymes, such as erythromycin esterase A, out of the cell membrane. We are characterizing these three signal sequences as part of our project this year in order to better understand their functions. Although the Parts Registry outlines where the different signal sequences move the protein to in the cell, it is hard to visually see the protein. In addition we aim to characterize these signal sequences with confocal microscopy to see how effectively they work under different environmental conditions.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p class="ContentParagraph">K331007 relates specifically to our project as it is the signal sequence already present in beta-lactamase. K331009 also relates as it will export our other antibiotic degrading enzymes, such as erythromycin esterase A, out of the cell membrane. We are characterizing these three signal sequences as part of our project this year in order to better understand their functions. Although the Parts Registry outlines where the different signal sequences move the protein to in the cell, it is hard to visually see the protein. In addition we aim to characterize these signal sequences with confocal microscopy to see how effectively they work under different environmental conditions.</p></div></td></tr>
</table>T Danghttp://2014hs.igem.org/wiki/index.php?title=Team:Lethbridge_Canada/parts&diff=28067&oldid=prevT Dang at 21:58, 2 July 20142014-07-02T21:58:00Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p class="ContentParagraph">K331007 relates specifically to our project as it is the signal sequence already present in beta-lactamase. K331009 also relates as it will export our other antibiotic degrading enzymes, such as erythromycin esterase A, out of the cell membrane. We are characterizing these three signal sequences as part of our project this year in order to better understand their functions. Although the Parts Registry outlines where the different signal sequences move the protein to in the cell, it is hard to visually see the protein. In addition we aim to characterize these signal sequences with confocal microscopy to see how effectively they work under different environmental conditions.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p class="ContentParagraph">K331007 relates specifically to our project as it is the signal sequence already present in beta-lactamase. K331009 also relates as it will export our other antibiotic degrading enzymes, such as erythromycin esterase A, out of the cell membrane. We are characterizing these three signal sequences as part of our project this year in order to better understand their functions. Although the Parts Registry outlines where the different signal sequences move the protein to in the cell, it is hard to visually see the protein. In addition we aim to characterize these signal sequences with confocal microscopy to see how effectively they work under different environmental conditions.</p></div></td></tr>
</table>T Danghttp://2014hs.igem.org/wiki/index.php?title=Team:Lethbridge_Canada/parts&diff=28066&oldid=prevT Dang at 21:57, 2 July 20142014-07-02T21:57:15Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p class="ContentParagraph">K331007 relates specifically to our project as it is the signal sequence already present in beta-lactamase. K331009 also relates as it will export our other antibiotic degrading enzymes, such as erythromycin esterase A, out of the cell membrane. We are characterizing these three signal sequences as part of our project this year in order to better understand their functions. Although the Parts Registry outlines where the different signal sequences move the protein to in the cell, it is hard to visually see the protein. In addition we aim to characterize these signal sequences with confocal microscopy to see how effectively they work under different environmental conditions.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p class="ContentParagraph">K331007 relates specifically to our project as it is the signal sequence already present in beta-lactamase. K331009 also relates as it will export our other antibiotic degrading enzymes, such as erythromycin esterase A, out of the cell membrane. We are characterizing these three signal sequences as part of our project this year in order to better understand their functions. Although the Parts Registry outlines where the different signal sequences move the protein to in the cell, it is hard to visually see the protein. In addition we aim to characterize these signal sequences with confocal microscopy to see how effectively they work under different environmental conditions.</p></div></td></tr>
</table>T Danghttp://2014hs.igem.org/wiki/index.php?title=Team:Lethbridge_Canada/parts&diff=28065&oldid=prevT Dang at 21:56, 2 July 20142014-07-02T21:56:37Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p class="ContentParagraph">K331007 relates specifically to our project as it is the signal sequence already present in beta-lactamase. K331009 also relates as it will export our other antibiotic degrading enzymes, such as erythromycin esterase A, out of the cell membrane. We are characterizing these three signal sequences as part of our project this year in order to better understand their functions. Although the Parts Registry outlines where the different signal sequences move the protein to in the cell, it is hard to visually see the protein. In addition we aim to characterize these signal sequences with confocal microscopy to see how effectively they work under different environmental conditions.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p class="ContentParagraph">K331007 relates specifically to our project as it is the signal sequence already present in beta-lactamase. K331009 also relates as it will export our other antibiotic degrading enzymes, such as erythromycin esterase A, out of the cell membrane. We are characterizing these three signal sequences as part of our project this year in order to better understand their functions. Although the Parts Registry outlines where the different signal sequences move the protein to in the cell, it is hard to visually see the protein. In addition we aim to characterize these signal sequences with confocal microscopy to see how effectively they work under different environmental conditions.</p></div></td></tr>
</table>T Danghttp://2014hs.igem.org/wiki/index.php?title=Team:Lethbridge_Canada/parts&diff=27840&oldid=prevStinsonz at 03:53, 21 June 20142014-06-21T03:53:57Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014hs/a/aa/LethHS2014_Full_Construct_Image.png" style="text-align:center; margin-bottom:15px" width="930px" height="400px"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014hs/a/aa/LethHS2014_Full_Construct_Image.png" style="text-align:center; margin-bottom:15px" width="930px" height="400px"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p class="ContentParagraph">K331007 relates specifically to our project as it is the signal sequence already present in beta-lactamase. K331009 also relates as it will export our other antibiotic degrading enzymes, such as erythromycin esterase A, out of the cell membrane. We are characterizing these three signal sequences as part of our project this year in order to better understand their functions. Although the Parts Registry outlines where the different signal sequences move the protein to in the cell, it is hard to visually see the protein. In addition we aim to characterize these signal sequences with confocal microscopy to see how <del class="diffchange diffchange-inline">effective </del>they work under different environmental conditions.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p class="ContentParagraph">K331007 relates specifically to our project as it is the signal sequence already present in beta-lactamase. K331009 also relates as it will export our other antibiotic degrading enzymes, such as erythromycin esterase A, out of the cell membrane. We are characterizing these three signal sequences as part of our project this year in order to better understand their functions. Although the Parts Registry outlines where the different signal sequences move the protein to in the cell, it is hard to visually see the protein. In addition we aim to characterize these signal sequences with confocal microscopy to see how <ins class="diffchange diffchange-inline">effectively </ins>they work under different environmental conditions.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p class="ContentParagraph">Our construct also uses J04500 (IPTG inducible promoter with RBS) as our promoter as it is a well characterized promoter, and was used by the 2012 Lethbridge High School iGEM team for their insulin secretion construct. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p class="ContentParagraph">Our construct also uses J04500 (IPTG inducible promoter with RBS) as our promoter as it is a well characterized promoter, and was used by the 2012 Lethbridge High School iGEM team for their insulin secretion construct. </p></div></td></tr>
</table>Stinsonzhttp://2014hs.igem.org/wiki/index.php?title=Team:Lethbridge_Canada/parts&diff=27817&oldid=prevStinsonz at 03:52, 21 June 20142014-06-21T03:52:45Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014hs/a/aa/LethHS2014_Full_Construct_Image.png" style="text-align:center; margin-bottom:15px" width="930px" height="400px"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014hs/a/aa/LethHS2014_Full_Construct_Image.png" style="text-align:center; margin-bottom:15px" width="930px" height="400px"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p class="ContentParagraph">K331007 relates specifically to our project as it is the signal sequence already <del class="diffchange diffchange-inline">tagged onto the protein</del>. K331009 also relates as it will export our <del class="diffchange diffchange-inline">gene of interest beta-lactamase and </del>erythromycin esterase A out of the cell membrane <del class="diffchange diffchange-inline">-which is the purpose of our water treatment system</del>. We are <del class="diffchange diffchange-inline">characterising </del>these three signal sequences as part of our project this year in order to better understand their functions. Although the Parts Registry outlines where the different signal sequences <del class="diffchange diffchange-inline">export </del>the protein in the cell, it is hard to visually see the protein. In addition we aim to <del class="diffchange diffchange-inline">characterise </del>these signal sequences to see how effective they work under different environmental conditions.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p class="ContentParagraph">K331007 relates specifically to our project as it is the signal sequence already <ins class="diffchange diffchange-inline">present in beta-lactamase</ins>. K331009 also relates as it will export our <ins class="diffchange diffchange-inline">other antibiotic degrading enzymes, such as </ins>erythromycin esterase A<ins class="diffchange diffchange-inline">, </ins>out of the cell membrane. We are <ins class="diffchange diffchange-inline">characterizing </ins>these three signal sequences as part of our project this year in order to better understand their functions. Although the Parts Registry outlines where the different signal sequences <ins class="diffchange diffchange-inline">move </ins>the protein <ins class="diffchange diffchange-inline">to </ins>in the cell, it is hard to visually see the protein. In addition we aim to <ins class="diffchange diffchange-inline">characterize </ins>these signal sequences <ins class="diffchange diffchange-inline">with confocal microscopy </ins>to see how effective they work under different environmental conditions.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p class="ContentParagraph">Our construct also uses J04500 (IPTG inducible promoter with RBS) as our promoter as it is a well characterized promoter, and was used by the 2012 Lethbridge High School iGEM team for their insulin secretion construct. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p class="ContentParagraph">Our construct also uses J04500 (IPTG inducible promoter with RBS) as our promoter as it is a well characterized promoter, and was used by the 2012 Lethbridge High School iGEM team for their insulin secretion construct. </p></div></td></tr>
</table>Stinsonzhttp://2014hs.igem.org/wiki/index.php?title=Team:Lethbridge_Canada/parts&diff=27688&oldid=prevSallyxu1234567 at 03:41, 21 June 20142014-06-21T03:41:20Z<p></p>
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</table>Sallyxu1234567http://2014hs.igem.org/wiki/index.php?title=Team:Lethbridge_Canada/parts&diff=26969&oldid=prevT Dang at 02:42, 21 June 20142014-06-21T02:42:23Z<p></p>
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</table>T Danghttp://2014hs.igem.org/wiki/index.php?title=Team:Lethbridge_Canada/parts&diff=26900&oldid=prevYoyo.Yao at 02:34, 21 June 20142014-06-21T02:34:58Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p class="ContentParagraph">K331007 relates specifically to our project as it is the signal sequence already tagged onto the protein. K331009 also relates as it will export our gene of interest beta-lactamase and erythromycin esterase A out of the cell membrane -which is the purpose of our water treatment system. We are characterising these three signal sequences as part of our project this year in order to better understand their functions. Although the Parts Registry outlines where the different signal sequences export the protein in the cell, it is hard to visually see the protein. In addition we aim to characterise these signal sequences to see how effective they work under different environmental conditions.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p class="ContentParagraph">K331007 relates specifically to our project as it is the signal sequence already tagged onto the protein. K331009 also relates as it will export our gene of interest beta-lactamase and erythromycin esterase A out of the cell membrane -which is the purpose of our water treatment system. We are characterising these three signal sequences as part of our project this year in order to better understand their functions. Although the Parts Registry outlines where the different signal sequences export the protein in the cell, it is hard to visually see the protein. In addition we aim to characterise these signal sequences to see how effective they work under different environmental conditions.</p></div></td></tr>
</table>Yoyo.Yaohttp://2014hs.igem.org/wiki/index.php?title=Team:Lethbridge_Canada/parts&diff=26711&oldid=prevDinula98 at 02:19, 21 June 20142014-06-21T02:19:44Z<p></p>
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