Revision as of 22:59, 20 June 2014

Lambert iGEM NoteBook

Fall 2013: Our team visited a local farm to determine needs in our community. From the visit, we began formulating the idea of a fruit preservative.
November and December 2013: We began to work on sterile techniques and safety training. Also began research into Chitin to Chitosan Pathway as well as applications of chitosan.
January 2014: Began research into source organisms for the Chitin Deacytelase gene.
February 2014: iGEM kit arrived.
March 2014: Began extracting genomic yeast DNA. Used a protocol from Looke Article, Biotechnology Volume 50. using lithium acetate etc. Results were inconsistent and yields were low.
April 2014: Used Pierce Thermoscientific Yeast DNA Extraction Kit to extract genomic DNA from S.cerevisiae. PCR using pMAL primers. Began Ligations.
May 2014: Continued ligations using Quick Ligation, Overnight ligation and T4ligase. Further research determined low efficiency of blunt ended ligations.
June 2014: Began Biobricking CDA gene. Designed Biobrick primers for CDA, PCR, digested, ligated with PSB1C3 and transformations with DH10beta competent cells.

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           <li> <b>February 2014:</b> iGEM kit arrived.</li>
           <li> <b>March 2014:</b> Began extracting genomic yeast DNA.  Used a protocol from Looke Article, Biotechnology Volume 50.  using lithium acetate etc.  Results were inconsistent and yields were low.</li>
-</li><b> April 2014:<b> Used Pierce Thermoscientific Yeast DNA Extraction Kit to extract genomic DNA from S.cerevisiae. PCR using pMAL primers.  Began Ligations.</li>
+<li><b> April 2014:</b> Used Pierce Thermoscientific Yeast DNA Extraction Kit to extract genomic DNA from S.cerevisiae. PCR using pMAL primers.  Began Ligations.</li>
-<li><b> May 2014:<b> Continued ligations using Quick Ligation, Overnight ligation and T4ligase.  Further research determined low efficiency of blunt ended ligations.</li>
+<li><b> May 2014:</b> Continued ligations using Quick Ligation, Overnight ligation and T4ligase.  Further research determined low efficiency of blunt ended ligations.</li>
-<li><b> June 2014:<b> Began Biobricking CDA gene. Designed Biobrick primers for CDA, PCR, digested, ligated with PSB1C3 and transformations with DH10beta competent cells.</li>
+<li><b> June 2014:</b> Began Biobricking CDA gene. Designed Biobrick primers for CDA, PCR, digested, ligated with PSB1C3 and transformations with DH10beta competent cells.</li>
        </ul>