Team:HUNGENIOUS/trial mit

From 2014hs.igem.org

(Difference between revisions)
 
(11 intermediate revisions not shown)
Line 411: Line 411:
#top-section, #top-section * { border: none !important; border: none;}
#top-section, #top-section * { border: none !important; border: none;}
-
#footer-box { left: -5px }
+
#footer-box { widht: 953px  }
#accordion h3 { margin-bottom: 1px; }
#accordion h3 { margin-bottom: 1px; }
#catlinks { display: none;}
#catlinks { display: none;}
Line 608: Line 608:
<div style="float:left; clear:left;  margin-top: -2.5em; z-index:1; position:relative;">
<div style="float:left; clear:left;  margin-top: -2.5em; z-index:1; position:relative;">
-
<a href="https://2013.igem.org">
+
<a href="https://2014hs.igem.org">
<img src="https://static.igem.org/mediawiki/2012/7/79/IGEM-100px.png" alt="iGEM 2012">
<img src="https://static.igem.org/mediawiki/2012/7/79/IGEM-100px.png" alt="iGEM 2012">
</a>
</a>
Line 619: Line 619:
<h1 style="border: none; line-height: 1.2em; margin: 0; padding: 0; padding-top: 0.7em;">
<h1 style="border: none; line-height: 1.2em; margin: 0; padding: 0; padding-top: 0.7em;">
-
Treatment of Crohn's Disease by Probiotic Bacteria
+
Treatment of Crohn's Disease by Probiotic E.coli Bacteria
</h1>
</h1>
</a>
</a>
Line 625: Line 625:
<ul class="nav">
<ul class="nav">
-
<li id="nav_motivation"><a href="https://2013.igem.org/Team:MIT/Motivation">BACKGROUND</a>
+
<li id="nav_motivation"><a href="https://2014hs.igem.org/Team:HUNGENIOUS">HOME</a>
  <ul>
  <ul>
  </ul>
  </ul>
</li>
</li>
-
<li id="nav_results"><a href="https://2013.igem.org/Team:MIT/Project">RESULTS</a>
+
<li id="nav_results"><a href="https://2014hs.igem.org/Team:HUNGENIOUS/Project">TEAM</a>
  <ul>
  <ul>
-
   <li><a href="https://2013.igem.org/Team:MIT/Project">Project Overview</a></li>
+
   <li><a href="https://2014hs.igem.org/Team:HUNGENIOUS/Members">Members</a></li>
-
   <li><a href="https://2013.igem.org/Team:MIT/miRNA">miRNA Signal</a></li>
+
   <li><a href="https://2014hs.igem.org/Team:HUNGENIOUS/Mentors">Mentors</a></li>
-
   <li><a href="https://2013.igem.org/Team:MIT/Protein">Protein Signals</a></li>
+
   <li><a href="https://2014hs.igem.org/Team:HUNGENIOUS/Attributions">Atrributions</a></li>
-
   <li><a href="https://2013.igem.org/Team:MIT/Venus">Novel DNA Sensor: Cas9 Split Venus Fusion</a></li>
+
   <li><a href="https://2014hs.igem.org/Team:HUNGENIOUS/Pictures">Pictures</a></li>
-
  <li><a href="https://2013.igem.org/Team:MIT/ResultsBiobricks">BioBricks</a></li>
+
  </ul>
-
  <li><a href="https://2013.igem.org/Team:MIT/Attributions">Attributions</a></li>
+
-
</ul>
+
</li>
</li>
-
<li id="nav_methods"><a href="https://2013.igem.org/Team:MIT/MaterialsAndMethods">METHODS</a>
+
<li id="nav_methods"><a href="https://2014hs.igem.org/Team:HUNGENIOUS/Summary">PROJECT</a>
  <ul>
  <ul>
 +
  <li><a href="https://2014hs.igem.org/Team:HUNGENIOUS/Summary">Summary</a></li>
 +
  <li><a href="https://2014hs.igem.org/Team:HUNGENIOUS/Article">Article</a></li>
  </ul>
  </ul>
</li>
</li>
-
<li id="nav_notebook"><a href="https://2013.igem.org/Team:MIT/Notebook">NOTEBOOK</a>
 
-
<ul>
 
-
  <li><a onclick="nav_notebook('construction')">Construction &amp; <em>In Vitro</em></a></li>
 
-
  <li><a onclick="nav_notebook('summertc')">Summer Tissue Culture</a></li>
 
-
  <li><a onclick="nav_notebook('falltc')">Fall Tissue Culture</a></li>
 
-
</ul>
 
-
</li>
 
-
<li id="nav_human_practices"><a href="https://2013.igem.org/Team:MIT/HumanPractices">HUMAN PRACTICES</a>
+
<li id="nav_human_practices"><a href="https://2014hs.igem.org/Team:HUNGENIOUS/HumanPractices">HUMAN PRACTICES</a>
  <ul>
  <ul>
 +
  <li><a href="https://2014hs.igem.org/Team:HUNGENIOUS/Crohn's disease">Crohn's disease</a></li>
 +
  <li><a href="https://2014hs.igem.org/Team:HUNGENIOUS/Survey">Survey</a></li>
 +
  <li><a href="https://2014hs.igem.org/Team:HUNGENIOUS/Nobel Prize">Nobel Prize</a></li>
  </ul>
  </ul>
</li>
</li>
-
<li id="nav_safety"><a href="https://2013.igem.org/Team:MIT/Safety">SAFETY</a>
+
<li id="nav_safety"><a href="https://2014hs.igem.org/Team:HUNGENIOUS/Safety">LAB</a>
  <ul>
  <ul>
 +
  <li><a href="https://2014hs.igem.org/Team:HUNGENIOUS/Safety">Safety</a></li>
 +
  <li><a href="https://2014hs.igem.org/Team:HUNGENIOUS/Methods">Methods</a></li>
 +
  <li><a href="https://2014hs.igem.org/Team:HUNGENIOUS/Venues">Venues</a></li>
 +
  <li><a href="https://2014hs.igem.org/Team:HUNGENIOUS/Bioinfo">Bioinfo</a></li>
  </ul>
  </ul>
</li>
</li>
-
<li id="nav_team"><a href="https://2013.igem.org/Team:MIT/Team">TEAM</a>
 
-
<ul>
 
-
</ul>
 
-
</li>
 
-
 
-
<li id="nav_partners"><a href="https://2013.igem.org/Team:MIT/Partners">SPONSORS</a>
 
-
<ul>
 
-
</ul>
 
-
</li>
 
-
 
-
<li id="nav_contact"><a href="https://2013.igem.org/Team:MIT/Contact">CONTACT US</a>
 
-
<ul>
 
-
</ul>
 
-
</li>
 
</ul>
</ul>
-
</body>
 
-
<head>
 
-
<script type="text/javascript" src="http://konami-js.googlecode.com/svn/trunk/konami.js"></script>
 
-
<script>
 
-
konami = new Konami();
 
-
document.title = "MIT iGEM - Home";
 
-
konami.load("http://gobarbra.com/hit/new-93fa9e27c975c039c9eadefa578aa2be");
 
-
</script>
 
-
</head>
 
-
<body>
 
<div id="col_center">
<div id="col_center">
     <div id="block-content">
     <div id="block-content">
     </div><!--end block-content-->
     </div><!--end block-content-->
-
<img src="https://static.igem.org/mediawiki/2013/0/05/Front_page_overview.png" width="100%" >
+
 
<h1>Motivation</h1>
<h1>Motivation</h1>
Line 751: Line 727:
-->
-->
-
<!-- Saved in parser cache with key 2013_igem_org:pcache:idhash:411-0!1!0!!en!2!edit=0 and timestamp 20140606201311 -->
+
<!-- end content -->
-
<div class="printfooter">
+
-
Retrieved from "<a href="https://2013.igem.org/Team:MIT">https://2013.igem.org/Team:MIT</a>"</div>
+
-
<div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div></div> <!-- end content -->
+
-
<div class="visualClear"></div>
+
-
</div>
+
-
    </div>
+
-
<!-- PAGE FOOTER -- ITEMS FROM COLUMN ! HAVE BEEN MOVED HERE  -- RDR  -->
+
-
<div class="visualClear"></div>
+
-
    <div id='footer-box' class='noprint'>
+
-
        <div id="footer">
+
-
              <div id="f-poweredbyico"><a href="http://www.mediawiki.org/"><img src="/wiki/skins/common/images/poweredby_mediawiki_88x31.png" height="31" width="88" alt="Powered by MediaWiki" /></a></div>       <div id="f-copyrightico"><a href="http://creativecommons.org/licenses/by/3.0/"><img src="http://i.creativecommons.org/l/by/3.0/88x31.png" alt="Attribution 3.0 Unported" width="88" height="31" /></a></div>     <ul id="f-list">
+
-
+
-
  <!-- Recentchanges is not handles well DEBUG -->
+
-
    <li id="t-recentchanges"><a href="/Special:RecentChanges"
+
-
      title='Recent changes'>Recent changes</a></li>
+
-
 
+
-
    <li id="t-whatlinkshere"><a href="/Special:WhatLinksHere/Team:MIT"
+
-
      title="List of all wiki pages that link here [j]" accesskey="j">What links here</a></li>
+
-
 
+
-
                        <li id="t-recentchangeslinked"><a href="/Special:RecentChangesLinked/Team:MIT"
+
-
                          title="Recent changes in pages linked from this page [k]" accesskey="k">Related changes</a></li>
+
-
 
+
-
 
+
-
 
+
-
                <li id="t-specialpages"><a href="/Special:SpecialPages"
+
-
                  title="List of all special pages [q]" accesskey="q">Special pages</a>
+
-
                </li>
+
-
                <li><a href='/Special:Preferences'>My preferences</a></li>
+
-
            </ul>
+
-
        </div> <!-- close footer -->
+
-
        <div id='footer'>
+
-
    <ul id="f-list">
+
-
 
+
-
            <li id="t-print"><a href="/wiki/index.php?title=Team:MIT&amp;printable=yes"
+
-
              title="Printable version of this page [p]" accesskey="p">Printable version</a>
+
-
            </li>
+
-
 
+
-
            <li id="t-permalink"><a href="/wiki/index.php?title=Team:MIT&amp;oldid=361530"
+
-
              title="Permanent link to this revision of the page">Permanent link</a>
+
-
            </li>
+
-
 
+
-
 
+
-
        <li id="privacy"><a href="/2013.igem.org:Privacy_policy" title="2013.igem.org:Privacy policy">Privacy policy</a></li>
+
-
        <li id="disclaimer"><a href="/2013.igem.org:General_disclaimer" title="2013.igem.org:General disclaimer">Disclaimers</a></li>
+
-
    </ul>
+
-
</div> <!-- close footer -->
+
-
    </div> <!-- close footer-box -->
+
-
+
-
<script>if (window.runOnloadHook) runOnloadHook();</script>
+
-
</div>
+
-
<!-- Served in 0.164 secs. --></body>
+
-
 
+
-
 
+
-
</p>
+
-
<!--
+
-
NewPP limit report
+
-
Preprocessor node count: 4/1000000
+
-
Post-expand include size: 0/2097152 bytes
+
-
Template argument size: 0/2097152 bytes
+
-
Expensive parser function count: 0/100
+
-
-->
+
-
 
+
-
<!-- Saved in parser cache with key 2014hs_igem_org:pcache:idhash:1150-0!1!0!!en!2 and timestamp 20140607151733 -->
+
-
<div class="printfooter">
+
-
Retrieved from "<a href="https://2014hs.igem.org/Team:HUNGENIOUS/trial_mit">https://2014hs.igem.org/Team:HUNGENIOUS/trial_mit</a>"</div>
+
-
<div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div></div> <!-- end content -->
+
-
<div class="visualClear"></div>
+
-
</div>
+
-
    </div>
+
-
<!-- PAGE FOOTER -- ITEMS FROM COLUMN ! HAVE BEEN MOVED HERE  -- RDR  -->
+
-
<div class="visualClear"></div>
+
-
    <div id='footer-box' class='noprint'>
+
-
        <div id="footer">
+
-
              <div id="f-poweredbyico"><a href="http://www.mediawiki.org/"><img src="/wiki/skins/common/images/poweredby_mediawiki_88x31.png" height="31" width="88" alt="Powered by MediaWiki" /></a></div>       <div id="f-copyrightico"><a href="http://creativecommons.org/licenses/by/3.0/"><img src="http://i.creativecommons.org/l/by/3.0/88x31.png" alt="Attribution 3.0 Unported" width="88" height="31" /></a></div>     <ul id="f-list">
+
-
 
-
  <!-- Recentchanges is not handles well DEBUG -->
 
-
    <li id="t-recentchanges"><a href="/Special:RecentChanges"
 
-
      title='Recent changes'>Recent changes</a></li>
 
-
 
-
    <li id="t-whatlinkshere"><a href="/Special:WhatLinksHere/Team:HUNGENIOUS/trial_mit"
 
-
      title="List of all wiki pages that link here [j]" accesskey="j">What links here</a></li>
 
-
 
-
                        <li id="t-recentchangeslinked"><a href="/Special:RecentChangesLinked/Team:HUNGENIOUS/trial_mit"
 
-
                          title="Recent changes in pages linked from this page [k]" accesskey="k">Related changes</a></li>
 
-
 
-
 
-
 
-
                <li id="t-upload"><a href="/Special:Upload"
 
-
                  title="Upload files [u]" accesskey="u">Upload file</a>
 
-
                </li>
 
-
                <li id="t-specialpages"><a href="/Special:SpecialPages"
 
-
                  title="List of all special pages [q]" accesskey="q">Special pages</a>
 
-
                </li>
 
-
                <li><a href='/Special:Preferences'>My preferences</a></li>
 
-
            </ul>
 
-
        </div> <!-- close footer -->
 
-
        <div id='footer'>
 
-
    <ul id="f-list">
 
-
 
-
            <li id="t-print"><a href="/wiki/index.php?title=Team:HUNGENIOUS/trial_mit&amp;printable=yes"
 
-
              title="Printable version of this page [p]" accesskey="p">Printable version</a>
 
-
            </li>
 
-
 
-
            <li id="t-permalink"><a href="/wiki/index.php?title=Team:HUNGENIOUS/trial_mit&amp;oldid=9325"
 
-
              title="Permanent link to this revision of the page">Permanent link</a>
 
-
            </li>
 
-
 
-
 
-
        <li id="privacy"><a href="/2014hs.igem.org:Privacy_policy" title="2014hs.igem.org:Privacy policy">Privacy policy</a></li>
 
-
        <li id="disclaimer"><a href="/2014hs.igem.org:General_disclaimer" title="2014hs.igem.org:General disclaimer">Disclaimers</a></li>
 
-
    </ul>
 
-
</div> <!-- close footer -->
 
-
    </div> <!-- close footer-box -->
 
 
 
<script>if (window.runOnloadHook) runOnloadHook();</script>
<script>if (window.runOnloadHook) runOnloadHook();</script>
</div>
</div>
-
<!-- Served in 0.155 secs. --></body>
+
<!-- Served in 0 secs. --></body>
</html>
</html>

Latest revision as of 17:41, 7 June 2014

Team:HUNGENIOUS/trial mit - 2014hs.igem.org

Team:HUNGENIOUS/trial mit

From 2014hs.igem.org

Team:MIT - 2013.igem.org

Motivation

This summer, the 2013 MIT iGEM team worked to engineer exosome mediated cell-cell communication. In vivo cell-cell communication is vital for pattern formation, organ development, coordinated responses to environmental changes, and the maintenance of an organism (Bacchus, 2012)

Our Contribution

We demonstrated that exosomes can be engineered to transport protein and miRNA signals of interest. These signals remain functional and can actuate a response in a receiver cell. By co-culturing sender and receiver cells, we have demonstrated exosome mediated unidirectional cell-cell communication.

Our Vision

Exosomes provide an innovative means of engineering cell-cell communication that can have exciting application in drug testing and development. Tissue engineers are currently working to develop organoids small tissue structures that recapitulate the behavior of organs in vitro (Lancaster, 2013). Organoids can be used to test drugs more rigorously in a human-like context rather than relying solely on animal models. Thus drugs can be developed with a better understanding of their toxicity and efficacy. These multicellular structures require cell-cell communication, and our exosome mediated communication system could serve as an enabling technology for organoid development

Lancaster, Madeline et al. Cerebral organoids model human brain development and microcephaly. Nature 501, 373–379 (2013) Bacchus, William et al. Synthetic two-way communication between mammalian cells. Nat. Biotechnol. 30, 991–996 (2012)

Sponsors