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Team:GenetiX Tec CCM - Revision history
2024-03-29T13:26:11Z
Revision history for this page on the wiki
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172.110.62.33 at 14:24, 16 February 2016
2016-02-16T14:24:54Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> administrative and practical aspects of the project. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> administrative and practical aspects of the project. </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <div class="miembro"><h4 class="8u center">Name Removed</h4></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <div class="miembro"><h4 class="8u center">L. Daniel Aragón B.</h4></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <p><img src="https://static.igem.org/mediawiki/2014hs/7/76/Emilio.png" alt=""</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> class="float left" height="250" width="250"> A.K.A.:</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline">Grandpa&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; <br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> Semester: Fifth Semester<br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> I am a 17 year old Mexican high school student. I am one of the</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> most recent members of GENETIX, where I am currently developing</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> strategies for the promotion of our team at Tecnológico de</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> Monterrey and other educational institutions by creating social</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> media profiles that show our progress along with the production of</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> the uniform by supervising the creation of our shirts (regarding</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> materials and embroidery) and by establishing connections with</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> authorities inside the campus. The reason I am working with</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> GENETIX is because, even though I want to study Law, I have always</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> found Science very interesting, I believe in the team and I care</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> for its members. </p></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </div></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><div class="miembro"><h4 class="8u center">L. Daniel Aragón B.</h4></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p><img src="https://static.igem.org/mediawiki/2014hs/c/c8/Daniel.png" alt=""</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p><img src="https://static.igem.org/mediawiki/2014hs/c/c8/Daniel.png" alt=""</div></td></tr>
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172.110.62.33
http://2014hs.igem.org/wiki/index.php?title=Team:GenetiX_Tec_CCM&diff=27932&oldid=prev
Gonzila at 04:01, 21 June 2014
2014-06-21T04:01:00Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> reactions. The lower bands located in 3,4,7,8 were cut and used as inserts</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> reactions. The lower bands located in 3,4,7,8 were cut and used as inserts</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> inside the oxygen and iron promoters in the ligation reactions. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> inside the oxygen and iron promoters in the ligation reactions. </p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline"><p align="center"></del><img src="https://static.igem.org/mediawiki/2014hs/0/0d/Gel1.png<del class="diffchange diffchange-inline">></p</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014hs/0/0d/Gel1.png></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline"><p align="center"></del><img src="https://static.igem.org/mediawiki/2014hs/e/e9/Gel2.png<del class="diffchange diffchange-inline">></p</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img src="https://static.igem.org/mediawiki/2014hs/e/e9/Gel2.png></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="center"><img src="https://static.igem.org/mediawiki/2014hs/1/17/Results.png" alt="" class="8u center" height="360"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="center"><img src="https://static.igem.org/mediawiki/2014hs/1/17/Results.png" alt="" class="8u center" height="360"</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> administrative and practical aspects of the project. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> administrative and practical aspects of the project. </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <div class="miembro"><h4 class="8u center"><del class="diffchange diffchange-inline">Emilio Adame Valencia</del></h4></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <div class="miembro"><h4 class="8u center"><ins class="diffchange diffchange-inline">Name Removed</ins></h4></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p><img src="https://static.igem.org/mediawiki/2014hs/7/76/Emilio.png" alt=""</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p><img src="https://static.igem.org/mediawiki/2014hs/7/76/Emilio.png" alt=""</div></td></tr>
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Gonzila
http://2014hs.igem.org/wiki/index.php?title=Team:GenetiX_Tec_CCM&diff=27892&oldid=prev
Gonzila at 03:58, 21 June 2014
2014-06-21T03:58:30Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> points distributed all along Xochimilco lake. Results of these</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> points distributed all along Xochimilco lake. Results of these</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> tests could be then applied in the construction a map of the lake</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> tests could be then applied in the construction a map of the lake</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> specifying the most critical points that need to be treated.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> specifying the most critical points that need to be treated. <ins class="diffchange diffchange-inline">Agarose </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> gel showing the results from restriction reactions. We used a 1kb </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> DNA weight ladder (5) for measuring our fragments. The restriction </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> reactions from the O2 promoter plasmid (1,2) were done using Spel </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> and Pstl. The oxygen promoter has a ~ 155 bp weight. The restriction </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> reactions from the plasmids containing the GFP were cut with Xbal and</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> Pstl (3,4). GFP can be identified by the weight of 761 bp. The iron </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> promoter plasmid is cut with Spel and Pstl with a weight of 1067 bp (6).</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> Finally RFP plasmid was cut using Xbal and Pstl with a weight of 747 </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> bp (7,8). We can notice that the oxygen and iron promoters are cut with</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> the same restriction enzymes, as the GFP and RFP also are also cut in the </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> same restriction sites.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> We decided to use the higher bands in 1,2,6 corresponding to the oxygen </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> and iron promoters as the receptor plasmids for our subsequent ligation </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> reactions. The lower bands located in 3,4,7,8 were cut and used as inserts</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> inside the oxygen and iron promoters in the ligation reactions. </p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <p align="center"><img src="https://static.igem.org/mediawiki/2014hs/0/0d/Gel1.png></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <p align="center"><img src="https://static.igem.org/mediawiki/2014hs/e/e9/Gel2.png></ins></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="center"><img src="https://static.igem.org/mediawiki/2014hs/1/17/Results.png" alt="" class="8u center" height="360"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="center"><img src="https://static.igem.org/mediawiki/2014hs/1/17/Results.png" alt="" class="8u center" height="360"</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> width="500"></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> width="500"></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="justify" class="8u center">Figure 1. The agarose gel shows the unsuccesful</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p align="justify" class="8u center">Figure 1. The agarose gel shows the unsuccesful</div></td></tr>
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Gonzila
http://2014hs.igem.org/wiki/index.php?title=Team:GenetiX_Tec_CCM&diff=27674&oldid=prev
Heroghost at 03:40, 21 June 2014
2014-06-21T03:40:32Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Daniel Rodriguez<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Daniel Rodriguez<br></div></td></tr>
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Heroghost
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Gonzila at 03:37, 21 June 2014
2014-06-21T03:37:48Z
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Gonzila
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Heroghost at 02:58, 21 June 2014
2014-06-21T02:58:28Z
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2014-06-21T02:52:33Z
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Heroghost
http://2014hs.igem.org/wiki/index.php?title=Team:GenetiX_Tec_CCM&diff=26381&oldid=prev
Heroghost at 01:53, 21 June 2014
2014-06-21T01:53:26Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><title>GenetiX</title></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><title>GenetiX</title></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2 align="justify" id="project">The Project</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2 align="justify" id="project">The Project</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align="center"><a href="#"><img src="https://static.igem.org/mediawiki/2014hs/<del class="diffchange diffchange-inline">e</del>/<del class="diffchange diffchange-inline">ea</del>/<del class="diffchange diffchange-inline">Project</del>.jpg" alt="banner image" width="900"></a></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align="center"><a href="#"><img src="https://static.igem.org/mediawiki/2014hs/<ins class="diffchange diffchange-inline">f</ins>/<ins class="diffchange diffchange-inline">f2</ins>/<ins class="diffchange diffchange-inline">Genetix_project.jpg</ins>.jpg" alt="banner image" width="900"></a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="justify">The main idea of our project is to achieve the detection of</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align="justify">The main idea of our project is to achieve the detection of</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> anoxia and Iron concentrations in water systems. What we propose</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> anoxia and Iron concentrations in water systems. What we propose</div></td></tr>
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Heroghost
http://2014hs.igem.org/wiki/index.php?title=Team:GenetiX_Tec_CCM&diff=26331&oldid=prev
Heroghost at 01:48, 21 June 2014
2014-06-21T01:48:36Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><title>GenetiX</title></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><title>GenetiX</title></div></td></tr>
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Heroghost
http://2014hs.igem.org/wiki/index.php?title=Team:GenetiX_Tec_CCM&diff=26317&oldid=prev
Heroghost at 01:47, 21 June 2014
2014-06-21T01:47:37Z
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Heroghost