Team:FHS Frederick MD/Procedures

TEAM Description Members and Attributions Official Profile PROJECT Overview Microbial Fuel Cells NirB Promoter LOV Domain RESEARCH Materials and Methods Lab Notebook Safety RESULTS Transformations Electrophoresis Sequencing Outlook HUMAN PRACTICES Renewable Energy Clean Water Basic Research Public Awareness SPONSORS

3A Assembly

We used the 3A, or 3 antibody, assembly kit in order to transform E.coli with two genes, the LOV gene and the NirB gene. These genes will allow for further work with Schwenella bacteria in the anaerobic microbial fuel cell. We then used the mini-prep components of the kit to purify our plasmid. We verified the plasmid's presence through electrophoresis and further sequence analysis.