Team:FHS Frederick MD/Procedures

From 2014hs.igem.org

(Difference between revisions)
(Added header)
(Moved here from the main page.)
Line 1: Line 1:
{{:Team:FHS_Frederick_MD/Header}}
{{:Team:FHS_Frederick_MD/Header}}
 +
 +
====3A Assembly====
 +
 +
We used the 3A, or 3 antibody, assembly kit in order to transform E.coli with two genes, the LOV gene and the NirB gene.  These genes  will allow for further work with Schwenella bacteria in the anaerobic microbial fuel cell.  We then used the mini-prep components of the kit to purify our plasmid. We verified the plasmid's presence through electrophoresis and further  sequence analysis.

Revision as of 10:51, 19 June 2014

FHS Logo.png


3A Assembly

We used the 3A, or 3 antibody, assembly kit in order to transform E.coli with two genes, the LOV gene and the NirB gene. These genes will allow for further work with Schwenella bacteria in the anaerobic microbial fuel cell. We then used the mini-prep components of the kit to purify our plasmid. We verified the plasmid's presence through electrophoresis and further sequence analysis.
Retrieved from "http://2014hs.igem.org/Team:FHS_Frederick_MD/Procedures"