Team:FHS Frederick MD/Notebook

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Lab Notebook

The following links will direct you to the [http://arsbiotechnica.org Ars Biotechnica] website, which houses all the notes on the experiments we performed during the last year to reach our goal of purifying the plasmids that were transformed into our E. coli bacteria.


  • [http://arsbiotechnica.org/w/Article:002-001 20 Feb 2014]: Received iGEM supplies
  • [http://arsbiotechnica.org/w/Record:002-010 03 Apr 2014]: Digested NirB and LOV inserts
  • [http://arsbiotechnica.org/w/Record:002-011 10 Apr 2014]: Ligated NirB and LOV genes into pSB1C3
  • [http://arsbiotechnica.org/w/Record:002-012 14 Apr 2014]: Transformed E. coli with LOV and NirB constructs
  • [http://arsbiotechnica.org/w/Record:002-013 21 Apr 2014]: Ligated NirB and LOV genes into pSB1C3
  • [http://arsbiotechnica.org/w/Record:002-014 28 Apr 2014]: Transformed E. coli with LOV and NirB constructs
  • [http://arsbiotechnica.org/w/Record:002-015 01 May 2014]: Prepared TSS buffer
  • [http://arsbiotechnica.org/w/Record:002-016 05 May 2014]: Ligatied the RFP plasmid
  • [http://arsbiotechnica.org/w/Record:002-017 08 May 2014]: Subcultured E. coli NEB10 Beta
  • [http://arsbiotechnica.org/w/Record:002-018 08 May 2014]: Tested transformation efficiency of competent cells
  • [http://arsbiotechnica.org/w/Record:002-020 12 May 2014]: Digested NirB, LOV, pSB1C3 DNA fragments with EcoRI and PstI
  • [http://arsbiotechnica.org/w/Record:002-021 15 May 2014]: Ligated NirB and LOV into pSB1C3
  • [http://arsbiotechnica.org/w/Record:002-022 20 May 2014]: Transformed E. coli with LOV and NirB constructs
  • [http://arsbiotechnica.org/w/Record:002-023 29 May 2014]: Cultured individual NirB and LOV colonies
  • [http://arsbiotechnica.org/w/Record:002-024 02 Jun 2014]: Purified NirB and LOV plasmids
  • [http://arsbiotechnica.org/w/Record:002-025 03 Jun 2014]: Visualized NirB and LOV plasmids on a gel
  • [http://arsbiotechnica.org/w/Record:002-026 04 Jun 2014]: Recultured NirB and LOV transformants
  • [http://arsbiotechnica.org/w/Record:002-027 05 Jun 2014]: Extracted plasmid DNA from bacterial transformants
  • [http://arsbiotechnica.org/w/Record:002-028 06 Jun 2014]: Observed plasmid DNA on a gel
  • [http://arsbiotechnica.org/w/Record:002-029 09 Jun 2014]: Submitted samples for sequencing
  • [http://arsbiotechnica.org/w/Record:002-030 11 Jun 2014]: Resubmitted DNA for sequencing
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