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Team:FHS Frederick MD/Notebook
From 2014hs.igem.org
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* [http://arsbiotechnica.org/w/Article:002-001 20 Feb 2014]: Received iGEM supplies | * [http://arsbiotechnica.org/w/Article:002-001 20 Feb 2014]: Received iGEM supplies | ||
Revision as of 01:01, 21 June 2014
Lab Notebook
- 20 Feb 2014: Received iGEM supplies
- 03 Apr 2014: Digested NirB and LOV inserts
- 10 Apr 2014: Ligated NirB and LOV genes into pSB1C3
- 14 Apr 2014: Transformed E. coli with LOV and NirB constructs
- 21 Apr 2014: Ligated NirB and LOV genes into pSB1C3
- 28 Apr 2014: Transformed E. coli with LOV and NirB constructs
- 01 May 2014: Prepared TSS buffer
- 05 May 2014: Ligatied the RFP plasmid
- 08 May 2014: Subcultured E. coli NEB10 Beta
- 08 May 2014: Tested transformation efficiency of competent cells
- 12 May 2014: Digested NirB, LOV, pSB1C3 DNA fragments with EcoRI and PstI
- 15 May 2014: Ligated NirB and LOV into pSB1C3
- 20 May 2014: Transformed E. coli with LOV and NirB constructs
- 29 May 2014: Cultured individual NirB and LOV colonies
- 02 Jun 2014: Purified NirB and LOV plasmids
- 03 Jun 2014: Visualized NirB and LOV plasmids on a gel
- 04 Jun 2014: Recultured NirB and LOV transformants
- 05 Jun 2014: Extracted plasmid DNA from bacterial transformants
- 06 Jun 2014: Observed plasmid DNA on a gel
- 09 Jun 2014: Submitted samples for sequencing
- 11 Jun 2014: Resubmitted DNA for sequencing
