Team:FHS Frederick MD/NirB Promoter


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NirB Promoter

The NirB gene is reliant on a fumarate and nitrate reductase (FNR), which allows the promoter to activate when there is no oxygen present, as well as facilitate regulation of transcription that is responsible for the growth under anaerobic conditions. When oxygen is not present, the 4Fe-4S complex helps join two FNR components. As this happens, it becomes a protein complex that attaches to the NirB promoter part of the DNA, which results in the production of the LOV protein.

In an experiment by the 2012 Valencia Biocampus iGEM team (see BBa_K763002), sterile oil was glossed over the tube containing the bacteria. The bacteria were then able to use the oxygen until the levels were completely depleted, resulting in a fully anaerobic environment. Once fully anaerobic, the bacteria began to produce the team's fluorescent gene product, which was under the control of the NirB promoter.

We will similarly use the oxygen-sensitive NirB promoter to regulate transcription of the fluorescent LOV protein that we are designing.