Team:FHS Frederick MD/NirB Promoter


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NirB Promoter

The NirB gene is reliant on a fumarate and nitrate reductase (FNR), which allows the promoter to activate when there is no oxygen present, as well as facilitates in the regulation of transcription that is responsible for the growth under anaerobic conditions. When oxygen is not present, the 4Fe-4S complex helps join the FNR components. As this happens, it becomes a protein that attaches to the NirB part of the DNA, which results in the production of the LOV gene.

In an experiment by Morales, sterile oil was glossed over the tube containing the bacteria. The bacteria were then able to use the oxygen until the levels were completely depleted, resulting in a fully anaerobic environment. Once fully anaerobic, the bacteria continued to produce the LOV gene product.

Morales, Pedro Luis Dorado. "pNirB + Gene encoding ZsGreen1." . (accessed June 16, 2014).