Team:FHS Frederick MD/Lab Notes

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Notebook

The following link will direct you to the ArsBiotechnica website which houses all the protocols we followed through the last year to reach our goals of purifying the plasmid the was transformed into our E.coli bacteria

http://arsbiotechnica.org/w/Procedures

  • [http://arsbiotechnica.org/w/Article:002-001 20 Feb 2014]: Received iGEM supplies
  • [http://arsbiotechnica.org/w/Record:002-010 03 Apr 2014]: Digested NirB and LOV inserts
  • [http://arsbiotechnica.org/w/Record:002-011 10 Apr 2014]: Ligated NirB and LOV genes into pSB1C3
  • [http://arsbiotechnica.org/w/Record:002-012 14 Apr 2014]: Transformed E. coli with LOV and NirB constructs
  • [http://arsbiotechnica.org/w/Record:002-013 21 Apr 2014]: Ligated NirB and LOV genes into pSB1C3
  • [http://arsbiotechnica.org/w/Record:002-014 28 Apr 2014]: Transformed E. coli with LOV and NirB constructs
  • [http://arsbiotechnica.org/w/Record:002-015 01 May 2014]: Prepared TSS buffer
  • [http://arsbiotechnica.org/w/Record:002-016 05 May 2014]: Ligatied the RFP plasmid
  • [http://arsbiotechnica.org/w/Record:002-017 08 May 2014]: Subcultured E. coli NEB10 Beta
  • [http://arsbiotechnica.org/w/Record:002-018 08 May 2014]: Tested transformation efficiency of competent cells
  • [http://arsbiotechnica.org/w/Record:002-020 12 May 2014]: Digested NirB, LOV, pSB1C3 DNA fragments with EcoRI and PstI
  • [http://arsbiotechnica.org/w/Record:002-021 15 May 2014]: Ligated NirB and LOV into pSB1C3
  • [http://arsbiotechnica.org/w/Record:002-022 20 May 2014]: Transformed E. coli with LOV and NirB constructs
  • [http://arsbiotechnica.org/w/Record:002-023 29 May 2014]: Cultured individual NirB and LOV colonies
  • [http://arsbiotechnica.org/w/Record:002-024 02 Jun 2014]: Purified NirB and LOV plasmids
  • [http://arsbiotechnica.org/w/Record:002-025 03 Jun 2014]: Visualized NirB and LOV plasmids on a gel
  • [http://arsbiotechnica.org/w/Record:002-026 04 Jun 2014]: Recultured NirB and LOV transformants
  • [http://arsbiotechnica.org/w/Record:002-027 05 Jun 2014]: Extracted plasmid DNA from bacterial transformants
  • [http://arsbiotechnica.org/w/Record:002-028 06 Jun 2014]: Observed plasmid DNA on a gel
  • [http://arsbiotechnica.org/w/Record:002-029 09 Jun 2014]: Submitted samples for sequencing
  • [http://arsbiotechnica.org/w/Record:002-030 11 Jun 2014]: Resubmitted DNA for sequencing
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