Lab Notes

The following link will direct you to the ArsBiotechnica website which houses all the protocols we followed through the last year to reach our goals of purifying the plasmid the was transformed into our E.coli bacteria

http://arsbiotechnica.org/w/Procedures

• 20 Feb 2014: Received iGEM supplies
• 03 Apr 2014: Digested NirB and LOV inserts
• 10 Apr 2014: Ligated NirB and LOV genes into pSB1C3
• 14 Apr 2014: Transformed E. coli with LOV and NirB constructs
• 21 Apr 2014: Ligated NirB and LOV genes into pSB1C3
• 28 Apr 2014: Transformed E. coli with LOV and NirB constructs
• 01 May 2014: Prepared TSS buffer
• 05 May 2014: Ligatied the RFP plasmid
• 08 May 2014: Subcultured E. coli NEB10 Beta
• 08 May 2014: Tested transformation efficiency of competent cells
• 12 May 2014: Digested NirB, LOV, pSB1C3 DNA fragments with EcoRI and PstI
• 15 May 2014: Ligated NirB and LOV into pSB1C3
• 20 May 2014: Transformed E. coli with LOV and NirB constructs
• 29 May 2014: Cultured individual NirB and LOV colonies
• 02 Jun 2014: Purified NirB and LOV plasmids
• 03 Jun 2014: Visualized NirB and LOV plasmids on a gel
• 04 Jun 2014: Recultured NirB and LOV transformants
• 05 Jun 2014: Extracted plasmid DNA from bacterial transformants
• 06 Jun 2014: Observed plasmid DNA on a gel
• 09 Jun 2014: Submitted samples for sequencing
• 11 Jun 2014: Resubmitted DNA for sequencing