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Team:Elan Vital South Korea/p results and conclusion
From 2014hs.igem.org
Results and Conclusion
From the investigation, we could conclude that MRSA showed strong resistance to ampicillin, gentamycin, and kanamycin, and slight resistance to tetracycline. The transformed E. coli grew in high concentrations of ampicillin, gentamycin, and kanamycin, showing high resistance to those drugs. Also, the transformed E. coli grew in low concentrations of tetracycline, but died in high concentration of the drug, showing low resistance to tetracycline. This shows that the DNA from the MRSA that transformed the E. coli codes for multidrug resistance. Furthermore, we were able to get an idea of which of the DNA were involved in the multidrug resistance. By running PCR, we were able to amplify the DNA involved, and by analyzing this, we could come up with a better understanding of how the multidrug resistance works. An appropriate follow up on the project would be to research the sequence of the identified genes with the hopes of finding a possible drug that destroys even the most drug-resistant MRSA
