Team:Charlottesville RS/Project/Applications

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<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Project"><span><span>Overall Project</span></span></a></li>
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<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Notebook/material"><span><span>Material & Methods</span></span></a>
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<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Notebook/material"><span><span>Material & Methods</span></span></a></li>
<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Project/Applications"><span><span>Applications</span></span></a></li>
<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Project/Applications"><span><span>Applications</span></span></a></li>
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<a href="https://2014hs.igem.org/Team:Charlottesville_RS/Parts" style="color: white">Parts<!--[if gt IE 6]><!--></a><!--<![endif]-->
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<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Parts"><span><span>Submitted Parts</span></span></a></li>
 
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== Moores Creek Wastewater Treatement Plant ==
== Moores Creek Wastewater Treatement Plant ==
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===Overview===
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The Moores Creek Wastewater Treatment Plant (WWTP) is managed by the Rivanna Water & Sewer Authority. The purpose of the water treatment plant is to use bacteria to purify waste water so it can be released into Moores Creek. Bacteria are used to process the waste and purify the water. However, these bacteria require nourishment, so every year the plant purchases $250,000 worth of glycerine in order to feed these bacteria. Our idea is to use genetically modified E. coli to produce a bioplastic called PHB, which the bacteria that purify the waste water could consume for energy, and thus save the city up to $250,000 on the Moores Creek WWTP and potentially apply to other WWTPs as well.
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The Moores Creek Wastewater Treatment Plant (WWTP) is managed by the Rivanna Water & Sewer Authority. The purpose of the water treatment plant is to use bacteria to purify waste water so it can be released back to the public. The bacteria process the waste and by extension purify the water. However, these bacteria need food, so every year the plant purchases $250,000 worth of food in order to feed these bacteria. Our idea is to use different bacteria to produce a bioplastic called PHB, which the bacteria that purify the waste water could use as food, and thus save the city $250,000 on the Moores plant and potentially apply to other WWTPs around the country.
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==How Wastewater Treatment Plants Work==
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'''1)''' Anoxic: Polyphosphate-accumulating organism (PAO’s) are able consume simple carbon compounds without the presence of an external electron acceptor, such as NO3 and O2. The bacteria in this step are deprived of NO3 and O2 to give the PAO’s a competitive advantage.  This is done to enhance the removal of phosphorus.
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'''2)''' Anaerobic: Denitrifying bacteria are supplied with NO3 from step 3 but are denied O2.  The bacteria can separate the NO3 into N2 and O2 and then use the O2.  (2NO3→N2+3O2)  The nitrogen is now a harmless gas that evaporates out of the wastewater.
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'''3)''' Aerobic: Aerators supply O2 and the bacteria convert NH3 from the wastewater into NO3 and H2.  The NO3 is recycled to step 2.
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'''4)''' Anaerobic: Any leftover NO3 is processed by denitrifying bacteria, but the food supply has been largely depleted and is supplemented with glycerin.
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'''5)''' Aerobic: Any remaining organic material is consumed by the bacteria.
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===How Water Treatment Plants Work===
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=== ===
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Primary disinfection occurs within the water treatment facility to kill or inactivate harmful viruses, bacteria, and microbial matter that may be in the water when it is pumped from the environment  into the treatment plant.  
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[[image:denitrification.png|thumb|450px|center]]
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GAC (Granular Activated Carbon-pure carbon heated to promote "active" sites which can adsorb pollutants)  removes trace organic matter remaining in the water after conventional filtration, thereby providing less matter for the free chlorine to react with to form disinfection byproducts
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The technology behind ET-1 Activated Clay is a sodiumbentonite modified to be hydrophobic and selectively oleophilic; essentially repelling water and seeking organics. A pure modified clay, not blended with anthracite coal or other fillers like other filtration media, ET-1 is designed for use in liquid phase filtration vessels. Functioning on the principle of adsorption through Ionic Bonding, ET-1 encapsulates organic molecules, trapping them in the platelets of the clay through this attraction.
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==Sequencing Batch Reactor==
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Since we do not have as much space as the Moores Creek Wastewater Treatment Plant, so we used a sequencing batch reactor, where instead of the water flowing from step to step, the water remains in the same location and aerators are turned on and off to alternate between aerobic and anaerobic zones.  Instead of just two aerobic and anaerobic zones, we used sixteen.

Latest revision as of 14:43, 20 June 2014

Moores Creek Wastewater Treatement Plant

The Moores Creek Wastewater Treatment Plant (WWTP) is managed by the Rivanna Water & Sewer Authority. The purpose of the water treatment plant is to use bacteria to purify waste water so it can be released into Moores Creek. Bacteria are used to process the waste and purify the water. However, these bacteria require nourishment, so every year the plant purchases $250,000 worth of glycerine in order to feed these bacteria. Our idea is to use genetically modified E. coli to produce a bioplastic called PHB, which the bacteria that purify the waste water could consume for energy, and thus save the city up to $250,000 on the Moores Creek WWTP and potentially apply to other WWTPs as well.

How Wastewater Treatment Plants Work

1) Anoxic: Polyphosphate-accumulating organism (PAO’s) are able consume simple carbon compounds without the presence of an external electron acceptor, such as NO3 and O2. The bacteria in this step are deprived of NO3 and O2 to give the PAO’s a competitive advantage. This is done to enhance the removal of phosphorus.


2) Anaerobic: Denitrifying bacteria are supplied with NO3 from step 3 but are denied O2. The bacteria can separate the NO3 into N2 and O2 and then use the O2. (2NO3→N2+3O2) The nitrogen is now a harmless gas that evaporates out of the wastewater.


3) Aerobic: Aerators supply O2 and the bacteria convert NH3 from the wastewater into NO3 and H2. The NO3 is recycled to step 2.


4) Anaerobic: Any leftover NO3 is processed by denitrifying bacteria, but the food supply has been largely depleted and is supplemented with glycerin.


5) Aerobic: Any remaining organic material is consumed by the bacteria.


Denitrification.png

Sequencing Batch Reactor

Since we do not have as much space as the Moores Creek Wastewater Treatment Plant, so we used a sequencing batch reactor, where instead of the water flowing from step to step, the water remains in the same location and aerators are turned on and off to alternate between aerobic and anaerobic zones. Instead of just two aerobic and anaerobic zones, we used sixteen.