Team:Charlottesville RS/Notebook/material

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Material

Buffers & Solution

Kits

Marker

Enzymes

Plasmidvectors

Synthetic oligonucleotides

Phages

Bacteria

Bacteria Growth Media

Methods

Preparing competent cells

Transformation of bacteria

Isolation of plasmid DNA by alkaline lysis (mini and maxiprep)

DNA amplification using polymerase chain reaction (PCR)

Purification of DNA from PCR reactions

Enzymatic hydrolysis of DNA by restriction enzymes

Agarose gel electrophoresis for separating DNA

Isolation of DNA fragments from an agarose gel

Ligation of dsDNA fragments

Lysing of bacteria by ultrasonication

Glycerol stock

Preparation of plating bacteria for bacteriophage experiments

Bacteriophage λ plaque assay

Preparing Stocks of Bacteriophage λ

Preparing of λ DNA

In vitro packaging of λ DNA

Colicin Activity Test

Lysis test

Sender Activity Test

LuxS Activity Test

Conjugation test

Receptor Polarization Test

Swarm Assays

Microscopy – Chemotaxis

Microscopy – Colicin Toxicity

Microscopy – Cell lysis

Cell Culture

Eucaryotic Killing Assay with Colicins

Flow Cytometer Acquisition