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Team:Charlottesville RS/Notebook/material - Revision history
2024-03-29T13:24:46Z
Revision history for this page on the wiki
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http://2014hs.igem.org/wiki/index.php?title=Team:Charlottesville_RS/Notebook/material&diff=22937&oldid=prev
Ingamename: /* Ligation of RFP, Alcohol Acetyltransferase I, and PSB1C3 */
2014-06-20T15:09:20Z
<p><span class="autocomment">Ligation of RFP, Alcohol Acetyltransferase I, and PSB1C3</span></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 15:09, 20 June 2014</td>
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<td colspan="2" class="diff-lineno">Line 414:</td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Ligation of RFP, Alcohol Acetyltransferase I, and PSB1C3===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Ligation of RFP, Alcohol Acetyltransferase I, and PSB1C3===</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Thawed </del>T4 DNA Ligase Reaction Buffer. <del class="diffchange diffchange-inline">Labeled </del>0.6mL as ''New Part''. <del class="diffchange diffchange-inline">Added </del>2uL of ''pSB1C3'' linearized plasmid backbone digest. <del class="diffchange diffchange-inline">Added </del>3.3uL from ''Part A'' digest. <del class="diffchange diffchange-inline">Added </del>3.9uL from ''Part B'' digest. <del class="diffchange diffchange-inline">Added </del>1uL of T4 DNA Ligase Reaction Buffer. <del class="diffchange diffchange-inline">Added </del>0.5uL of T4 DNA Ligase. <del class="diffchange diffchange-inline">Mixed </del>solution by pipetting , and centrifuged for 5 seconds. <del class="diffchange diffchange-inline">Labeled another </del>0.6mL tube as ''Ligation Control''. <del class="diffchange diffchange-inline">Added </del>2uL from ''RFP Control'' digest. <del class="diffchange diffchange-inline">Added </del>6.5uL of distilled water. <del class="diffchange diffchange-inline">Added </del>1uL of T4 DNA Ligase Reaction Buffer. <del class="diffchange diffchange-inline">Added </del>0.5uL of T4 DNA Ligase. <del class="diffchange diffchange-inline">Mixed </del>solution by pipetting, and <del class="diffchange diffchange-inline">centrifuged </del>for 5 seconds. <del class="diffchange diffchange-inline">Incubated both </del>tubes at 16˚C for 30 minutes, then at 80˚C for 20 minutes<del class="diffchange diffchange-inline">. Stored </del>at -20˚C.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The </ins>T4 DNA Ligase Reaction Buffer <ins class="diffchange diffchange-inline">was thawed</ins>. 0.6mL <ins class="diffchange diffchange-inline">was labeled </ins>as ''New Part''. 2uL of <ins class="diffchange diffchange-inline">the </ins>''pSB1C3'' linearized plasmid backbone digest <ins class="diffchange diffchange-inline">was added</ins>. 3.3uL <ins class="diffchange diffchange-inline">was added </ins>from ''Part A'' digest. 3.9uL <ins class="diffchange diffchange-inline">was added </ins>from ''Part B'' digest. 1uL of T4 DNA Ligase Reaction Buffer <ins class="diffchange diffchange-inline">was added</ins>. 0.5uL of T4 DNA Ligase <ins class="diffchange diffchange-inline">was also added</ins>. <ins class="diffchange diffchange-inline">The </ins>solution <ins class="diffchange diffchange-inline">was mixed </ins>by pipetting, and <ins class="diffchange diffchange-inline">by being </ins>centrifuged for 5 seconds. <ins class="diffchange diffchange-inline">Another </ins>0.6mL tube <ins class="diffchange diffchange-inline">was labeled </ins>as ''Ligation Control''. 2uL from ''RFP Control'' digest <ins class="diffchange diffchange-inline">was added</ins>. 6.5uL of distilled water <ins class="diffchange diffchange-inline">was added</ins>. 1uL of T4 DNA Ligase Reaction Buffer <ins class="diffchange diffchange-inline">was added</ins>. 0.5uL of T4 DNA Ligase <ins class="diffchange diffchange-inline">was added</ins>. <ins class="diffchange diffchange-inline">The </ins>solution <ins class="diffchange diffchange-inline">was mixed </ins>by pipetting, and <ins class="diffchange diffchange-inline">centrifuging </ins>for 5 seconds. <ins class="diffchange diffchange-inline">Both </ins>tubes <ins class="diffchange diffchange-inline">were incubated </ins>at 16˚C for 30 minutes, then at 80˚C for 20 minutes <ins class="diffchange diffchange-inline">and then stored </ins>at -20˚C.</div></td></tr>
</table>
Ingamename
http://2014hs.igem.org/wiki/index.php?title=Team:Charlottesville_RS/Notebook/material&diff=22920&oldid=prev
Ingamename: /* Digestion of RFP and Alcohol Acetyltransferase I */
2014-06-20T15:00:00Z
<p><span class="autocomment">Digestion of RFP and Alcohol Acetyltransferase I</span></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 15:00, 20 June 2014</td>
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<td colspan="2" class="diff-lineno">Line 411:</td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Digestion of RFP and Alcohol Acetyltransferase I===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Digestion of RFP and Alcohol Acetyltransferase I===</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Thawed </del>NEB Buffer 2 and BSA in room temperature water. <del class="diffchange diffchange-inline">Labeled four </del>0.6mL tubes: ''Part A'', ''Part B'', ''pSB1C3'', and ''RFP Control''. <del class="diffchange diffchange-inline">Added </del>500ng of DNA to appropriate tube. <del class="diffchange diffchange-inline">Added distilled </del>water to tubes for a total volume <del class="diffchange diffchange-inline">for </del>42.5uL in each tube. <del class="diffchange diffchange-inline">Pipetted </del>5uL of Buffer 2 <del class="diffchange diffchange-inline">to </del>each tube. <del class="diffchange diffchange-inline">Pipetted </del>0.5uL of BSA <del class="diffchange diffchange-inline">to </del>each tube. In ''Part A'' tube, <del class="diffchange diffchange-inline">added </del>1 uL of EcoRI enzyme and 1uL of SpeI enzyme. In ''Part B'' tube, <del class="diffchange diffchange-inline">added </del>1uL of XbaI enzyme, and 1uL of PstI enzyme. In the ''pSB1C3'' tube, <del class="diffchange diffchange-inline">added </del>1uL of EcoRI enzyme, and 1uL of PstI enzyme. In the ''RFP Control'' tube, <del class="diffchange diffchange-inline">added </del>1uL of EcoRI enzyme, 1uL of PstI enzyme. <del class="diffchange diffchange-inline">Mixed </del>solution by pipetting up and down, and <del class="diffchange diffchange-inline">spun </del>for 5 seconds in micro-centrifuge to collect mixture at the bottom of the tube. <del class="diffchange diffchange-inline">Incubated the </del>restriction digests at 37˚C for 30 minutes, then 80˚C for 20 minutes. <del class="diffchange diffchange-inline">Stored </del>at -20˚C.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>NEB Buffer 2 and BSA <ins class="diffchange diffchange-inline"> were thawed </ins>in room temperature water. <ins class="diffchange diffchange-inline">Four </ins>0.6mL tubes: ''Part A'', ''Part B'', ''pSB1C3'', and ''RFP Control''. 500ng of DNA <ins class="diffchange diffchange-inline">were added </ins>to <ins class="diffchange diffchange-inline">the </ins>appropriate tube. <ins class="diffchange diffchange-inline">Distilled </ins>water <ins class="diffchange diffchange-inline">was added </ins>to <ins class="diffchange diffchange-inline">the </ins>tubes for a total volume <ins class="diffchange diffchange-inline">of </ins>42.5uL in each tube. 5uL of Buffer 2 <ins class="diffchange diffchange-inline">was pippetted into </ins>each tube. 0.5uL of BSA <ins class="diffchange diffchange-inline">was pippetted into </ins>each tube. In ''Part A'' tube, 1 uL of EcoRI enzyme <ins class="diffchange diffchange-inline">was added </ins>and 1uL of SpeI enzyme <ins class="diffchange diffchange-inline">was also added</ins>. In ''Part B'' tube, 1uL of XbaI enzyme <ins class="diffchange diffchange-inline">was added</ins>, and 1uL of PstI enzyme <ins class="diffchange diffchange-inline">was also added</ins>. In the ''pSB1C3'' tube, 1uL of EcoRI enzyme <ins class="diffchange diffchange-inline"> was added</ins>, and 1uL of PstI enzyme <ins class="diffchange diffchange-inline">was also added</ins>. In the ''RFP Control'' tube, 1uL of EcoRI enzyme <ins class="diffchange diffchange-inline">was added</ins>, 1uL of PstI enzyme <ins class="diffchange diffchange-inline">was also added</ins>. <ins class="diffchange diffchange-inline">The </ins>solution <ins class="diffchange diffchange-inline">was mixed </ins>by pipetting up and down, and <ins class="diffchange diffchange-inline">spinning it </ins>for 5 seconds in <ins class="diffchange diffchange-inline">the </ins>micro-centrifuge to collect <ins class="diffchange diffchange-inline">the </ins>mixture at the bottom of the tube. <ins class="diffchange diffchange-inline">The </ins>restriction digests <ins class="diffchange diffchange-inline">were incubated </ins>at 37˚C for 30 minutes, then <ins class="diffchange diffchange-inline">at </ins>80˚C for 20 minutes. <ins class="diffchange diffchange-inline">They were then stored </ins>at -20˚C.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Ligation of RFP, Alcohol Acetyltransferase I, and PSB1C3===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Ligation of RFP, Alcohol Acetyltransferase I, and PSB1C3===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Thawed T4 DNA Ligase Reaction Buffer. Labeled 0.6mL as ''New Part''. Added 2uL of ''pSB1C3'' linearized plasmid backbone digest. Added 3.3uL from ''Part A'' digest. Added 3.9uL from ''Part B'' digest. Added 1uL of T4 DNA Ligase Reaction Buffer. Added 0.5uL of T4 DNA Ligase. Mixed solution by pipetting , and centrifuged for 5 seconds. Labeled another 0.6mL tube as ''Ligation Control''. Added 2uL from ''RFP Control'' digest. Added 6.5uL of distilled water. Added 1uL of T4 DNA Ligase Reaction Buffer. Added 0.5uL of T4 DNA Ligase. Mixed solution by pipetting, and centrifuged for 5 seconds. Incubated both tubes at 16˚C for 30 minutes, then at 80˚C for 20 minutes. Stored at -20˚C.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Thawed T4 DNA Ligase Reaction Buffer. Labeled 0.6mL as ''New Part''. Added 2uL of ''pSB1C3'' linearized plasmid backbone digest. Added 3.3uL from ''Part A'' digest. Added 3.9uL from ''Part B'' digest. Added 1uL of T4 DNA Ligase Reaction Buffer. Added 0.5uL of T4 DNA Ligase. Mixed solution by pipetting , and centrifuged for 5 seconds. Labeled another 0.6mL tube as ''Ligation Control''. Added 2uL from ''RFP Control'' digest. Added 6.5uL of distilled water. Added 1uL of T4 DNA Ligase Reaction Buffer. Added 0.5uL of T4 DNA Ligase. Mixed solution by pipetting, and centrifuged for 5 seconds. Incubated both tubes at 16˚C for 30 minutes, then at 80˚C for 20 minutes. Stored at -20˚C.</div></td></tr>
</table>
Ingamename
http://2014hs.igem.org/wiki/index.php?title=Team:Charlottesville_RS/Notebook/material&diff=22907&oldid=prev
Ingamename: /* Extraction of RFP and Alcohol Acetyltransferase I */
2014-06-20T14:53:46Z
<p><span class="autocomment">Extraction of RFP and Alcohol Acetyltransferase I</span></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 14:53, 20 June 2014</td>
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<td colspan="2" class="diff-lineno">Line 408:</td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Extraction of RFP and Alcohol Acetyltransferase I===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Extraction of RFP and Alcohol Acetyltransferase I===</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Spun down two </del>culture tubes for 3 minutes at 8000 rpm. <del class="diffchange diffchange-inline">Poured supernatant </del>into biological waste container. <del class="diffchange diffchange-inline">Pipetted </del>250uL of Buffer P1 into each 14mL culture tube, and <del class="diffchange diffchange-inline">resuspended </del>pellet. <del class="diffchange diffchange-inline">Labeled </del>1.7mL micro-<del class="diffchange diffchange-inline">centrifuge </del>tubes with respective part names, and transferred <del class="diffchange diffchange-inline">resuspended </del>parts in. <del class="diffchange diffchange-inline">Pipetted </del>250uL of Buffer P2 into each micro-centrifuge tube. <del class="diffchange diffchange-inline">Flipped </del>tubes to mix solution. <del class="diffchange diffchange-inline">Pipetted </del>350uL of Buffer N3 into each tube. <del class="diffchange diffchange-inline">Flipped </del>tubes to mix solution. <del class="diffchange diffchange-inline">Spun down </del>tubes in micro-centrifuge for 10 minutes at 13,000 rpm. <del class="diffchange diffchange-inline">Labeled </del>spin columns for each tube, and <del class="diffchange diffchange-inline">pipetted </del>supernatant to <del class="diffchange diffchange-inline">the </del>respective spin columns. <del class="diffchange diffchange-inline">Spun down </del>spin columns at 13,000 rpm for 1 minute. <del class="diffchange diffchange-inline">Poured </del>flow through into chemical waste beaker. <del class="diffchange diffchange-inline">Pipetted </del>500uL of Buffer PB into each spin <del class="diffchange diffchange-inline">column</del>. <del class="diffchange diffchange-inline">Spun down </del>spin columns at 13,000 rpm for 1 minute. <del class="diffchange diffchange-inline">Poured </del>flow through into chemical waste beaker. <del class="diffchange diffchange-inline">Pipetted </del>750uL of Buffer PE to each spin column. <del class="diffchange diffchange-inline">Spun down </del>spin columns at 13,000 rpm for 1 minute. <del class="diffchange diffchange-inline">Poured </del>flow through into chemical waste beaker. <del class="diffchange diffchange-inline">Spun down </del>spin columns at 13,000 rpm for 1 minute to remove remaining buffer. <del class="diffchange diffchange-inline">Labeled sterile </del>1.7mL micro-<del class="diffchange diffchange-inline">centrifuge </del>tubes with part names. <del class="diffchange diffchange-inline">Transferred the </del>appropriate filter tube to <del class="diffchange diffchange-inline">the </del>respective tubes. <del class="diffchange diffchange-inline">Pipetted </del>50uL of dH2O to each filter tube. <del class="diffchange diffchange-inline">Let </del>samples <del class="diffchange diffchange-inline">sit </del>for 1 minute, then spun down <del class="diffchange diffchange-inline">tubes </del>at 13,000 rpm for 1 minute. <del class="diffchange diffchange-inline">Stored </del>micro-<del class="diffchange diffchange-inline">centrifuge </del>tubes at 20˚C.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Two </ins>culture tubes <ins class="diffchange diffchange-inline">were spun down </ins>for 3 minutes at 8000 rpm. <ins class="diffchange diffchange-inline">The supernantant was poured </ins>into <ins class="diffchange diffchange-inline">a </ins>biological waste container. 250uL of Buffer P1 <ins class="diffchange diffchange-inline">was pipetted </ins>into each 14mL culture tube, and <ins class="diffchange diffchange-inline">the </ins>pellet <ins class="diffchange diffchange-inline">was resuspended</ins>. <ins class="diffchange diffchange-inline"> The </ins>1.7mL micro-<ins class="diffchange diffchange-inline">centrifuged </ins>tubes <ins class="diffchange diffchange-inline">were labeled </ins>with <ins class="diffchange diffchange-inline">their </ins>respective part names, and transferred <ins class="diffchange diffchange-inline">the </ins>parts <ins class="diffchange diffchange-inline">were resuspended </ins>in <ins class="diffchange diffchange-inline">it</ins>. 250uL of Buffer P2 <ins class="diffchange diffchange-inline">was pippeted </ins>into each micro-centrifuge tube. <ins class="diffchange diffchange-inline">The </ins>tubes <ins class="diffchange diffchange-inline">were flipped </ins>to mix <ins class="diffchange diffchange-inline">the </ins>solution. 350uL of Buffer N3 <ins class="diffchange diffchange-inline">was pippeted </ins>into each tube.<ins class="diffchange diffchange-inline">The </ins>tubes <ins class="diffchange diffchange-inline">were flipped </ins>to mix <ins class="diffchange diffchange-inline">the </ins>solution. <ins class="diffchange diffchange-inline">The </ins>tubes in <ins class="diffchange diffchange-inline">the </ins>micro-centrifuge <ins class="diffchange diffchange-inline">were spun down </ins>for 10 minutes at 13,000 rpm. <ins class="diffchange diffchange-inline">The </ins>spin columns <ins class="diffchange diffchange-inline">were labeled </ins>for each tube, and <ins class="diffchange diffchange-inline">the </ins>supernatant <ins class="diffchange diffchange-inline">was pipetted </ins>to <ins class="diffchange diffchange-inline">their </ins>respective spin columns. <ins class="diffchange diffchange-inline">The </ins>spin columns <ins class="diffchange diffchange-inline">were spun down </ins>at 13,000 rpm for 1 minute. <ins class="diffchange diffchange-inline">The </ins>flow through <ins class="diffchange diffchange-inline">was poured </ins>into <ins class="diffchange diffchange-inline">the </ins>chemical waste beaker. 500uL <ins class="diffchange diffchange-inline">was pipetted </ins>of Buffer PB into each <ins class="diffchange diffchange-inline">of the </ins>spin <ins class="diffchange diffchange-inline">columns</ins>. <ins class="diffchange diffchange-inline">The </ins>spin columns <ins class="diffchange diffchange-inline">were each spun down </ins>at 13,000 rpm for 1 minute. <ins class="diffchange diffchange-inline">The </ins>flow through <ins class="diffchange diffchange-inline">was poured </ins>into <ins class="diffchange diffchange-inline">the </ins>chemical waste beaker. 750uL <ins class="diffchange diffchange-inline">was pipetted </ins>of Buffer PE to each spin column. <ins class="diffchange diffchange-inline">The </ins>spin columns <ins class="diffchange diffchange-inline">were spun down </ins>at 13,000 rpm for 1 minute. <ins class="diffchange diffchange-inline">The </ins>flow through <ins class="diffchange diffchange-inline">was poured </ins>into <ins class="diffchange diffchange-inline">the </ins>chemical waste beaker. <ins class="diffchange diffchange-inline">The </ins>spin columns <ins class="diffchange diffchange-inline">were spun down </ins>at 13,000 rpm for 1 minute to remove <ins class="diffchange diffchange-inline">the </ins>remaining buffer. <ins class="diffchange diffchange-inline">The spin columns were labeled “sterile </ins>1.7mL micro-<ins class="diffchange diffchange-inline">centrifuge” </ins>tubes with <ins class="diffchange diffchange-inline">their respective </ins>part names. <ins class="diffchange diffchange-inline">The </ins>appropriate filter tube <ins class="diffchange diffchange-inline">was transferred </ins>to <ins class="diffchange diffchange-inline">their </ins>respective tubes. 50uL of dH2O <ins class="diffchange diffchange-inline">was pipetted </ins>to each filter tube. <ins class="diffchange diffchange-inline">The </ins>samples <ins class="diffchange diffchange-inline">sat </ins>for 1 minute, then <ins class="diffchange diffchange-inline">the tubes were </ins>spun down at 13,000 rpm for 1 minute. <ins class="diffchange diffchange-inline">The </ins>micro-<ins class="diffchange diffchange-inline">centrifuged </ins>tubes <ins class="diffchange diffchange-inline">were stored </ins>at 20˚C.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Digestion of RFP and Alcohol Acetyltransferase I===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Digestion of RFP and Alcohol Acetyltransferase I===</div></td></tr>
</table>
Ingamename
http://2014hs.igem.org/wiki/index.php?title=Team:Charlottesville_RS/Notebook/material&diff=22904&oldid=prev
Ingamename: /* Transformation of RFP and Alcohol Acetyltransferase I */
2014-06-20T14:53:27Z
<p><span class="autocomment">Transformation of RFP and Alcohol Acetyltransferase I</span></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 14:53, 20 June 2014</td>
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<td colspan="2" class="diff-lineno">Line 405:</td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Transformation of RFP and Alcohol Acetyltransferase I===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Transformation of RFP and Alcohol Acetyltransferase I===</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Labeled </del>tubes as ''Transformation Control'','' Ligation: New Part'', and ''Ligation Control''. <del class="diffchange diffchange-inline">Added 5uL </del>of RFP Control DNA <del class="diffchange diffchange-inline">into </del>''Transformation Control''. <del class="diffchange diffchange-inline">Added 2uL </del>of New Part ligation product <del class="diffchange diffchange-inline">into </del>''Ligation: New Part''. <del class="diffchange diffchange-inline">Added 2uL </del>of the RFP Control ligation product <del class="diffchange diffchange-inline">into </del>the ''Ligation Control''. <del class="diffchange diffchange-inline">Put </del>tubes on ice to pre-chill<del class="diffchange diffchange-inline">, and thawed one competent cell </del>tube on ice. <del class="diffchange diffchange-inline">Pipetted 50uL </del>of competent cells into each 2.0mL micro-centrifuge <del class="diffchange diffchange-inline">tube</del>. <del class="diffchange diffchange-inline">Incubated </del>DNA and cell mixtures on ice for 30 minutes. During incubation, <del class="diffchange diffchange-inline">preheated waterbath </del>to 42˚C. <del class="diffchange diffchange-inline">Placed </del>tubes <del class="diffchange diffchange-inline">into </del>waterbath for 60 seconds, then incubated on ice for 5 minutes. <del class="diffchange diffchange-inline">Added 200uL </del>of SOC media to each tube. <del class="diffchange diffchange-inline">Incubated </del>tubes at 37˚C for 2 hours. <del class="diffchange diffchange-inline">Pipetted 200uL </del>of each tube onto respective plates. <del class="diffchange diffchange-inline">Spread over </del>surface using glass beads. <del class="diffchange diffchange-inline">Incubated </del>plates <del class="diffchange diffchange-inline">facing </del>down <del class="diffchange diffchange-inline">for </del>37˚C for 12-14 hours.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The </ins>tubes <ins class="diffchange diffchange-inline">were labeled </ins>as ''Transformation Control'', ''Ligation: New Part'', and ''Ligation Control''. <ins class="diffchange diffchange-inline">Five uL </ins>of <ins class="diffchange diffchange-inline">''</ins>RFP Control DNA<ins class="diffchange diffchange-inline">'' was added to </ins>''Transformation Control'' <ins class="diffchange diffchange-inline">tube</ins>. <ins class="diffchange diffchange-inline">Two uL </ins>of <ins class="diffchange diffchange-inline">''</ins>New Part<ins class="diffchange diffchange-inline">'' </ins>ligation product <ins class="diffchange diffchange-inline">was added to </ins>''Ligation: New Part'' <ins class="diffchange diffchange-inline">tube</ins>. <ins class="diffchange diffchange-inline">Two uL </ins>of the <ins class="diffchange diffchange-inline">''</ins>RFP Control<ins class="diffchange diffchange-inline">'' </ins>ligation product <ins class="diffchange diffchange-inline">was added to </ins>the ''Ligation Control'' <ins class="diffchange diffchange-inline">tube</ins>. <ins class="diffchange diffchange-inline"> The </ins>tubes <ins class="diffchange diffchange-inline">were placed </ins>on ice to pre-chill<ins class="diffchange diffchange-inline">. One </ins>tube <ins class="diffchange diffchange-inline">of previously prepared competent cells was thawed and left </ins>on ice. <ins class="diffchange diffchange-inline"> Fifty uL </ins>of competent cells <ins class="diffchange diffchange-inline">were pipetted </ins>into each <ins class="diffchange diffchange-inline">of the </ins>2.0mL micro-centrifuge <ins class="diffchange diffchange-inline">tubes</ins>. <ins class="diffchange diffchange-inline"> The </ins>DNA and cell mixtures <ins class="diffchange diffchange-inline">were incubated </ins>on ice for 30 minutes. During incubation, <ins class="diffchange diffchange-inline">the water bath was heated </ins>to 42˚C. <ins class="diffchange diffchange-inline">The </ins>tubes <ins class="diffchange diffchange-inline">were placed in the </ins>waterbath for 60 seconds, then <ins class="diffchange diffchange-inline">were </ins>incubated on ice <ins class="diffchange diffchange-inline">again </ins>for 5 minutes. <ins class="diffchange diffchange-inline"> Two hundred uL </ins>of SOC media <ins class="diffchange diffchange-inline">was added </ins>to each tube. <ins class="diffchange diffchange-inline"> The </ins>tubes <ins class="diffchange diffchange-inline">were incubated </ins>at 37˚C for 2 hours. <ins class="diffchange diffchange-inline">Two hundred uL </ins>of each tube <ins class="diffchange diffchange-inline">was pipetted </ins>onto respective plates. <ins class="diffchange diffchange-inline">The liquid was spread on the surface of the plate </ins>surface using glass beads. <ins class="diffchange diffchange-inline">The </ins>plates <ins class="diffchange diffchange-inline">were incubated lates face </ins>down <ins class="diffchange diffchange-inline">at </ins>37˚C for 12-14 hours.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Extraction of RFP and Alcohol Acetyltransferase I===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Extraction of RFP and Alcohol Acetyltransferase I===</div></td></tr>
</table>
Ingamename
http://2014hs.igem.org/wiki/index.php?title=Team:Charlottesville_RS/Notebook/material&diff=22893&oldid=prev
Ingamename: /* Rehydration of RFP and Alcohol Acetyltransferase I */
2014-06-20T14:50:54Z
<p><span class="autocomment">Rehydration of RFP and Alcohol Acetyltransferase I</span></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 14:50, 20 June 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Rehydration of RFP and Alcohol Acetyltransferase I===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Rehydration of RFP and Alcohol Acetyltransferase I===</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Pipetted 10uL </del>of dH2O into the well of corresponding Biobrick-standard part. <del class="diffchange diffchange-inline">Pipetted </del>up and down, then <del class="diffchange diffchange-inline">let </del>sit for 5 minutes. <del class="diffchange diffchange-inline">Tranformed 5 </del>uL of the re-suspended DNA into <del class="diffchange diffchange-inline">desired </del>cells<del class="diffchange diffchange-inline">, plate</del>, and <del class="diffchange diffchange-inline">grew </del>overnight.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Ten uL </ins>of dH2O <ins class="diffchange diffchange-inline">were pipetted </ins>into the well of <ins class="diffchange diffchange-inline">the </ins>corresponding Biobrick-standard part. <ins class="diffchange diffchange-inline">This solution was pipetted </ins>up and down, then <ins class="diffchange diffchange-inline">it was left to </ins>sit <ins class="diffchange diffchange-inline"> </ins>for 5 minutes. <ins class="diffchange diffchange-inline"> Five </ins>uL of the re-suspended DNA <ins class="diffchange diffchange-inline">was transformed </ins>into <ins class="diffchange diffchange-inline"> competent </ins>cells<ins class="diffchange diffchange-inline">. The transformed cells were plated</ins>, and <ins class="diffchange diffchange-inline">were grown </ins>overnight.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Transformation of RFP and Alcohol Acetyltransferase I===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Transformation of RFP and Alcohol Acetyltransferase I===</div></td></tr>
</table>
Ingamename
http://2014hs.igem.org/wiki/index.php?title=Team:Charlottesville_RS/Notebook/material&diff=22886&oldid=prev
Ingamename: /* Preparing competent cells */
2014-06-20T14:49:58Z
<p><span class="autocomment">Preparing competent cells</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 14:49, 20 June 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Preparing competent cells===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Preparing competent cells===</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The bacteria were grown to log (or exponential) phase and 10mL of bacteria were</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The bacteria were grown to log (or exponential) phase and 10mL of bacteria were aliquoted into 15 mL sterile plastic tubes. cells were pelleted using a tabletop centrifuge at 7000 x g for 10 minutes, or ~4000 rpm. The supernatant was poured off into liquid waste container. the tube was tapped to loosen the cell pellet, and d 5 mL of cold CaCL2 solution were added to the pellet. The cell solution was incubated on ice for 20 minutes. After incubation, the cells were spun for 5 minutes at 5000 x g (2500 rpm). The supernatant was poured off into the liquid waste container. One mL of cold CaCl2 solution was added to the cells and the cells were resuspended<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>aliquoted into 15 mL sterile plastic tubes. cells were pelleted using a tabletop </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>centrifuge at 7000 x g for 10 minutes, or ~4000 rpm. The supernatant was poured </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>off into liquid waste container. the tube was tapped to loosen the cell pellet, and d 5 </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>mL of cold CaCL2 solution were added to the pellet. The cell solution was incubated </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>on ice for 20 minutes. After incubation, the cells were spun for 5 minutes at 5000 x </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>g (2500 rpm). The supernatant was poured off into the liquid waste container. One </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>mL of cold CaCl2 solution was added to the cells and the cells were resuspended</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Rehydration of RFP and Alcohol Acetyltransferase I===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Rehydration of RFP and Alcohol Acetyltransferase I===</div></td></tr>
</table>
Ingamename
http://2014hs.igem.org/wiki/index.php?title=Team:Charlottesville_RS/Notebook/material&diff=22877&oldid=prev
Ingamename: /* Preparing competent cells */
2014-06-20T14:49:15Z
<p><span class="autocomment">Preparing competent cells</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
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<td colspan='2' style="background-color: white; color:black;">Revision as of 14:49, 20 June 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Preparing competent cells===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Preparing competent cells===</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Before lab, </del>bacteria were grown to log (or exponential) phase and 10mL of bacteria were aliquoted into 15 mL sterile plastic tubes. <del class="diffchange diffchange-inline">Pelleted </del>cells using tabletop centrifuge at 7000 x g for 10 minutes, or ~4000 rpm. <del class="diffchange diffchange-inline">Supernatant </del>was poured off into liquid waste container. <del class="diffchange diffchange-inline">Tapped </del>the tube to loosen cell pellet, and <del class="diffchange diffchange-inline">pipetted </del>5 mL of cold CaCL2 solution. <del class="diffchange diffchange-inline">Incubated tube </del>on ice for 20 minutes. After incubation, <del class="diffchange diffchange-inline">spun </del>cells for 5 minutes at 5000 x g (2500 rpm). <del class="diffchange diffchange-inline">Poured off </del>supernatant into liquid waste container. <del class="diffchange diffchange-inline">Used pipette to add 1 </del>mL of cold CaCl2 solution and <del class="diffchange diffchange-inline">resuspended </del>cells<del class="diffchange diffchange-inline">.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The </ins>bacteria were grown to log (or exponential) phase and 10mL of bacteria were</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>aliquoted into 15 mL sterile plastic tubes. cells <ins class="diffchange diffchange-inline">were pelleted </ins>using <ins class="diffchange diffchange-inline">a </ins>tabletop </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>centrifuge at 7000 x g for 10 minutes, or ~4000 rpm. <ins class="diffchange diffchange-inline">The supernatant </ins>was poured </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>off into liquid waste container. the tube <ins class="diffchange diffchange-inline">was tapped </ins>to loosen <ins class="diffchange diffchange-inline">the </ins>cell pellet, and <ins class="diffchange diffchange-inline">d </ins>5 </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>mL of cold CaCL2 solution <ins class="diffchange diffchange-inline">were added to the pellet</ins>. <ins class="diffchange diffchange-inline">The cell solution was incubated </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>on ice for 20 minutes. After incubation, <ins class="diffchange diffchange-inline">the </ins>cells <ins class="diffchange diffchange-inline">were spun </ins>for 5 minutes at 5000 x </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>g (2500 rpm). <ins class="diffchange diffchange-inline">The </ins>supernatant <ins class="diffchange diffchange-inline">was poured off </ins>into <ins class="diffchange diffchange-inline">the </ins>liquid waste container. <ins class="diffchange diffchange-inline">One </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>mL of cold CaCl2 solution <ins class="diffchange diffchange-inline">was added to the cells </ins>and <ins class="diffchange diffchange-inline">the </ins>cells <ins class="diffchange diffchange-inline">were resuspended</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Rehydration of RFP and Alcohol Acetyltransferase I===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Rehydration of RFP and Alcohol Acetyltransferase I===</div></td></tr>
</table>
Ingamename
http://2014hs.igem.org/wiki/index.php?title=Team:Charlottesville_RS/Notebook/material&diff=22875&oldid=prev
Ingamename: /* Material */
2014-06-20T14:48:52Z
<p><span class="autocomment">Material</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">==Material==</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">===Buffers & Solution===</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">===Kits===</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">===Marker===</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">===Enzymes===</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">===Plasmidvectors===</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">===Synthetic oligonucleotides===</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">===Phages===</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">===Bacteria===</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">===Bacteria Growth Media===</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Methods==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Methods==</div></td></tr>
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Ingamename
http://2014hs.igem.org/wiki/index.php?title=Team:Charlottesville_RS/Notebook/material&diff=22632&oldid=prev
Ingamename at 13:48, 20 June 2014
2014-06-20T13:48:24Z
<p></p>
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Ingamename
http://2014hs.igem.org/wiki/index.php?title=Team:Charlottesville_RS/Notebook/material&diff=22621&oldid=prev
Ingamename at 13:45, 20 June 2014
2014-06-20T13:45:19Z
<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Notebook/material"><span><span>Material & Methods</span></span></a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Notebook/material"><span><span>Material & Methods</span></span></a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Project/Applications"><span><span>Applications</span></span></a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Project/Applications"><span><span>Applications</span></span></a></li></div></td></tr>
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Ingamename