"
Page
Discussion
View source
History
teams
Log in
Team:Charlottesville RS/Notebook/material
From 2014hs.igem.org
Revision as of 13:07, 2 May 2014 by
Ingamename
(
Talk
|
contribs
)
(
diff
)
← Older revision
|
Latest revision
(
diff
) |
Newer revision →
(
diff
)
Home
Team
Overview
Advisors
Project
Overall Project
Material & Methods
Applications
Parts
Submitted Parts
Characterization
Notebook
Material & Methods
Sponsors
Material
Buffers & Solution
Kits
Marker
Enzymes
Plasmidvectors
Synthetic oligonucleotides
Phages
Bacteria
Bacteria Growth Media
Methods
Preparing chemically competent cells
Transformation of bacteria
Isolation of plasmid DNA by alkaline lysis (mini and maxiprep)
DNA amplification using polymerase chain reaction (PCR)
Purification of DNA from PCR reactions
Enzymatic hydrolysis of DNA by restriction enzymes
Agarose gel electrophoresis for separating DNA
Isolation of DNA fragments from an agarose gel
Ligation of dsDNA fragments
Lysing of bacteria by ultrasonication
Glycerol stock
Preparation of plating bacteria for bacteriophage experiments
Bacteriophage λ plaque assay
Preparing Stocks of Bacteriophage λ
Preparing of λ DNA
In vitro packaging of λ DNA
Colicin Activity Test
Lysis test
Sender Activity Test
LuxS Activity Test
Conjugation test
Receptor Polarization Test
Swarm Assays
Microscopy – Chemotaxis
Microscopy – Colicin Toxicity
Microscopy – Cell lysis
Cell Culture
Eucaryotic Killing Assay with Colicins
Flow Cytometer Acquisition