Team:Charlottesville RS/Notebook/material

(Difference between revisions)

Revision as of 13:21, 2 May 2014

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 ===Preparing competent cells===
-===Transformation of bacteria===
+===Rehydration of RFP and Iso===
-===Isolation of plasmid DNA by alkaline lysis (mini and maxiprep)===
+===Transformation of RFP and Iso===
-===DNA amplification using polymerase chain reaction (PCR)===
+===Extraction of RFP and Iso===
-===Purification of DNA from PCR reactions===
+===Digestion of RFP and Iso===
-===Enzymatic hydrolysis of DNA by restriction enzymes===
+===Digestion of PSB1C3===
-===Agarose gel electrophoresis for separating DNA===
+===Ligation of RFP, Iso, and PSB1C3===
-===Isolation of DNA fragments from an agarose gel===
+===Transformation of RFP, Iso, and PSB1C3===
-===Ligation of dsDNA fragments===
-===Lysing of bacteria by ultrasonication===
-===Glycerol stock===
-===Preparation of plating bacteria for bacteriophage experiments===
-===Bacteriophage λ plaque assay===
-===Preparing Stocks of Bacteriophage λ===
-===Preparing of λ DNA===
-===In vitro packaging of λ DNA===
-===Colicin Activity Test===
-===Lysis test===
-===Sender Activity Test===
-===LuxS Activity Test===
-===Conjugation test===
-=== Receptor Polarization Test ===
-===Swarm Assays===
-===Microscopy – Chemotaxis===
-===Microscopy – Colicin Toxicity===
-===Microscopy – Cell lysis===
-===Cell Culture===
-===Eucaryotic Killing Assay with Colicins===
-===Flow Cytometer Acquisition===