Team:CSWProteens

From 2014hs.igem.org

(Difference between revisions)
(Prototype team page)
 
(206 intermediate revisions not shown)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
 
-
 
<html>
<html>
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #fe2b33; /*background-color: #fe2b33;*/">
+
<style>
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; /*color: #f6f6f6*/; padding: 5px;">
+
#contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading,.visualClear {display: none;} /*-- hides default wiki settings --*/
-
This is a template page. READ THESE INSTRUCTIONS.
+
</style>
-
</div>
+
<html xmlns="http://www.w3.org/1999/xhtml">
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; /*color: #f6f6f6*/; padding: 5px;">
+
 
-
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
+
<head>
-
</div>
+
<meta content="en-ca" http-equiv="Content-Language" />
-
<div id="warning" style="/*text-align: center;*/ font-weight: bold; font-size: small; /*color: #f6f6f6*/; padding: 5px;">
+
<meta content="text/html; charset=utf-8" http-equiv="Content-Type" />
-
You <strong>MUST</strong> have the following information on your wiki:
+
<script src="https://2014hs.igem.org/Team:CSWProteens/jquery.slides.min?action=raw&amp;ctype=text/javascript"></script>
-
<ul style="font-weight:normal;">
+
<style type="text/css">
-
<li>a team description</li>  
+
.auto-style1 {
-
<li>project description</li>
+
font-family: Arial;
-
<li>safety information (did your team take a safety training course? were you supervised in the lab?)</li>
+
font-size: 20pt;
-
<li>team attribution (who did what part of your project?)</li>
+
margin-top: 0px;
 +
margin-bottom: 0px;
 +
        margin-right: 0px;
 +
margin-left: 0px;
 +
}
 +
 
 +
.auto-style2 {
 +
        text-align: center;
 +
}
 +
 
 +
.auto-style3 {
 +
        text-align: center;
 +
font-family: Arial;
 +
font-size: 50pt;
 +
margin-top: 26px;
 +
margin-bottom: 0px;
 +
        color: #058C1D;
 +
}
 +
 
 +
.auto-style4 {
 +
        text-align: center;
 +
font-family: Arial;
 +
font-size: 19pt;
 +
margin-top: 0px;
 +
margin-bottom: 0px;
 +
        color: #058C1D;
 +
}
 +
 
 +
.auto-style5 {
 +
        margin-top: 0px
 +
}
 +
 
 +
.auto-style6 {
 +
        text-align: center;
 +
font-family: Arial;
 +
font-size: 50pt;
 +
margin-top: 10px;
 +
margin-bottom: 10px;
 +
        color: #058C1D;
 +
 
 +
.auto-style7 {
 +
font-family: Arial;
 +
font-size: 20pt;
 +
margin-top: 5px;
 +
margin-bottom: 5px;
 +
        margin-right: 0px;
 +
margin-left: 0px;
 +
}
 +
 
 +
#content{
 +
background-image:url(http://upload.wikimedia.org/wikipedia/commons/a/ac/White_flag_of_surrender.svg);
 +
}
 +
 
 +
 
 +
</style>
 +
<style>
 +
    body {
 +
      -webkit-font-smoothing: antialiased;
 +
      font: normal 15px/1.5 "Helvetica Neue", Helvetica, Arial, sans-serif;
 +
      color: #232525;
 +
      padding-top:70px;
 +
    }
 +
 
 +
    #slides {
 +
      display: none
 +
    }
 +
 
 +
    #slides .slidesjs-navigation {
 +
      margin-top:5px;
 +
    }
 +
 
 +
    a.slidesjs-next,
 +
    a.slidesjs-previous,
 +
    a.slidesjs-play,
 +
    a.slidesjs-stop {
 +
      background-image: url(img/btns-next-prev.png);
 +
      background-repeat: no-repeat;
 +
      display:block;
 +
      width:12px;
 +
      height:18px;
 +
      overflow: hidden;
 +
      text-indent: -9999px;
 +
      float: left;
 +
      margin-right:5px;
 +
    }
 +
 
 +
    a.slidesjs-next {
 +
      margin-right:10px;
 +
      background-position: -12px 0;
 +
    }
 +
 
 +
    a:hover.slidesjs-next {
 +
      background-position: -12px -18px;
 +
    }
 +
 
 +
    a.slidesjs-previous {
 +
      background-position: 0 0;
 +
    }
 +
 
 +
    a:hover.slidesjs-previous {
 +
      background-position: 0 -18px;
 +
    }
 +
 
 +
    a.slidesjs-play {
 +
      width:15px;
 +
      background-position: -25px 0;
 +
    }
 +
 
 +
    a:hover.slidesjs-play {
 +
      background-position: -25px -18px;
 +
    }
 +
 
 +
    a.slidesjs-stop {
 +
      width:18px;
 +
      background-position: -41px 0;
 +
    }
 +
 
 +
    a:hover.slidesjs-stop {
 +
      background-position: -41px -18px;
 +
    }
 +
 
 +
    .slidesjs-pagination {
 +
      margin: 7px 0 0;
 +
      float: right;
 +
      list-style: none;
 +
    }
 +
 
 +
    .slidesjs-pagination li {
 +
      float: left;
 +
      margin: 0 1px;
 +
    }
 +
 
 +
    .slidesjs-pagination li a {
 +
      display: block;
 +
      width: 13px;
 +
      height: 0;
 +
      padding-top: 13px;
 +
      background-image: url(/img/pagination.png);
 +
      background-position: 0 0;
 +
      float: left;
 +
      overflow: hidden;
 +
    }
 +
 
 +
    .slidesjs-pagination li a.active,
 +
    .slidesjs-pagination li a:hover.active {
 +
      background-position: 0 -13px
 +
    }
 +
 
 +
    .slidesjs-pagination li a:hover {
 +
      background-position: 0 -26px
 +
    }
 +
 
 +
    #slides a:link,
 +
    #slides a:visited {
 +
      color: #333
 +
    }
 +
 
 +
    #slides a:hover,
 +
    #slides a:active {
 +
      color: #9e2020
 +
    }
 +
 
 +
    .navbar {
 +
      overflow: hidden
 +
    }
 +
</style>
 +
<style type="text/css">
 +
@import url(http://fonts.googleapis.com/css?family=Arial);
 +
ul, li {
 +
z-index:999;
 +
margin: 0;
 +
padding: 0;
 +
}
 +
 
 +
#nav li > a {
 +
transition: all 0.2s ease-in-out;
 +
}
 +
 
 +
#nav {
 +
-webkit-box-shadow: 0px 0px 5px 3px rgba(0, 0, 0, 0.75);
 +
-moz-box-shadow:    0px 0px 5px 3px rgba(0, 0, 0, 0.75);
 +
box-shadow:        0px 0px 5px 3px rgba(0, 0, 75, 0.75);
 +
font-family: 'Dosis', sans-serif;
 +
font-size: 24px;
 +
text-align:center;
 +
background-color: #ffffff;
 +
}
 +
#nav a {
 +
color: #000000;
 +
text-decoration: none;
 +
}
 +
#nav li {
 +
display: inline;
 +
position: relative;
 +
}
 +
#nav li ul {
 +
position: absolute;
 +
left: -9999px;
 +
}
 +
#nav li a {
 +
display: inline-block;
 +
padding: 20px;
 +
}
 +
#nav li:hover a {
 +
  background-color: #000000;
 +
color: #ffffff;
 +
}
 +
#nav li:hover ul {
 +
left: 0;
 +
text-align: left;
 +
width: 200px;
 +
box-shadow: 0px 1px 1px #20d681;
 +
}
 +
#nav li:hover ul a {
 +
display: block;
 +
padding: 15px 30px 15px 30px;
 +
border-bottom: 1px solid #70a667;
 +
}
 +
#nav li:hover ul a:hover {
 +
background-color: #70a667;
 +
color: #40004b;
 +
}
 +
</style>
 +
<FONT COLOR=white>
 +
<meta content="blendTrans(Duration=3.0)" http-equiv="Page-Enter" />
 +
<meta content="revealTrans(Duration=3.0,Transition=2)" http-equiv="Site-Enter" />
 +
</head>
 +
<center><a href="https://2014hs.igem.org/Team:CSWProteens/project"><img alt="" class="auto-style11" height="200" src="https://static.igem.org/mediawiki/2014hs/c/cd/Plantifreeze.gif" width="200" /><img src="https://static.igem.org/mediawiki/2014hs/7/7e/CSW_Banner.png" width="500">
 +
</a>
 +
 
 +
<a href="https://2014hs.igem.org/Main_Page"><img alt="" class="auto-style5" height="200" src="https://static.igem.org/mediawiki/2014hs/2/26/Igem_grpyon.png" width="200" /></a> </p></center>
 +
 
 +
<body style=" color: #FFFFFF;background-color: #000000;  
 +
background-image: url('http://upload.wikimedia.org/wikipedia/commons/a/ac/White_flag_of_surrender.svg');">
 +
<ul id="nav">
 +
<li><a href="https://2014hs.igem.org/Team:CSWProteens"><b>Home</b></a></li>
 +
  <li>
 +
<a href="https://2014hs.igem.org/Team:CSWProteens/team"><b>Team</b></a>
 +
    <ul>
 +
<li><a href="https://2014hs.igem.org/Team:CSWProteens/team">Members</a></li>
 +
        <li><a href="http://www.csw.org/">The Cambridge School of Weston</a></li>
 +
      <li><a href="https://2014hs.igem.org/Team:CSWProteens/advisors">Advisors</a></li>
 +
      <li><a href="https://2014hs.igem.org/Team:CSWProteens/sponsors">Sponsors</a></li>
 +
      <li><a href="https://2014hs.igem.org/Team:CSWProteens/Attributions">Attributions</a></li>
 +
</ul>
 +
</li>
 +
<li>
 +
<a href="https://2014hs.igem.org/Team:CSWProteens/project"><b>Project</b></a>
 +
<ul>
 +
<li><a href="https://2014hs.igem.org/Team:CSWProteens/project/frostprotection">Frost Protection</a></li>
 +
<li><a href="https://2014hs.igem.org/Team:CSWProteens/project">Project Description</a></li>
 +
<li><a href="https://2014hs.igem.org/Team:CSWProteens/project/Methods">Methods</a></li>
 +
<li><a href="https://2014hs.igem.org/Team:CSWProteens/project/results">Results</a></li>
 +
<li><a href="https://2014hs.igem.org/Team:CSWProteens/project/discussion">Discussion</a></li>
 +
<li><a href="https://2014hs.igem.org/Team:CSWProteens/project/safety">Safety</a></li>
 +
</ul>
 +
</li>
 +
<li>
 +
<a href="https://2014hs.igem.org/Team:CSWProteens/notebook"><b>Notebook</b></a>
 +
<ul>
 +
<li><a href="https://2014hs.igem.org/Team:CSWProteens/notebook/">Lab Journal</a></li>
 +
<li><a href="https://2014hs.igem.org/Team:CSWProteens/notebook/protocols">Protocols</a></li>
 +
<li><a href="https://2014hs.igem.org/Team:CSWProteens/notebook/parts">Parts</a></li>
 +
                                <li><a href="https://2014hs.igem.org/Team:CSWProteens/notebook/references">References</a></li>
 +
</ul>
 +
</li>
 +
<li>
 +
<a href="https://2014hs.igem.org/Team:CSWProteens/humanpractices"><b>Outreach</b></a>
 +
<ul>
 +
</ul>
</ul>
-
You may also wish to add other page such as:
+
</li>
-
<ul style="font-weight:normal;">
+
<li>
-
<li>lab notebook</li>
+
<a href="https://2014hs.igem.org/Team:CSWProteens/contact"><b>Contact Us</b></a>
-
<li>sponsor information</li>
+
<ul>
-
<li>other information</li>
+
<li><a href="http://csw-igem.tumblr.com">Tumblr</a></li>
-
</ul>  
+
<li><a href="http://www.facebook.com/cswigem">Facebook</a></li>
-
REMEMBER, keep all of your pages within your teams namespace. <br><span style="font-weight:normal; font-style:italic;">Example: 2013hs.igem.org/Team:CSWProteens/Our_Pets</span>
+
                                <li><a href="mailto:hgoldsweig@csw.org?subject=iGEM">Email</a></li>
-
</div>
+
</ul>
-
</div>
+
</ul><center><p>
-
</html>
+
<a href="https://2014hs.igem.org/Team:CSWProteens/team"><img src="https://static.igem.org/mediawiki/2014hs/d/df/Teampics1.png" width="975px"></a>
 +
<h3><p>Welcome!<br> We are the CSW Proteens! <br>Scroll down to find more about our project! </center></h3>
-
<!-- *********************** End of the alert box *********************** -->
+
</td>
 +
<td bgColor="#FFFFFF"></td>
 +
</tr>
 +
</table>
 +
<!-- end of first block -->
-
{|align="justify"
+
<!-Block of content 1 -->
-
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
+
<table width="100%"  cellspacing="0" height="500px">
-
|[[Image:CSWProteens_logo.png|200px|right|frame]]
+
<tr><td  bgColor="#FEE5AD"></td>
-
|-
+
<td width="975px" align="center" bgColor="#FEE5AD" >
-
|
+
<table  width="975px"  cellspacing="0" height="500px">
-
''Tell us more about your project. Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
+
<tr>
-
|[[Image:CSWProteens_team.png|right|frame|Your team picture]]
+
-
|-
+
-
|
+
-
|align="center"|[[Team:CSWProteens | Team CSWProteens]]
+
-
|}
+
-
<!--- Team Information Link --->
+
<td width="95%" bgColor="#FEE5AD"  rowspan="3" align="center">  
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
<!-- Content goes here -->
-
!align="center"|[https://igem.org/Team.cgi?year=2013&division=high_school&team_name=CSWProteens Official Team Profile]
+
<img src="https://static.igem.org/mediawiki/2014/0/0a/DwikitemplateB_dnastrand.png">
-
|}
+
<h3>PlantiFreeze</h3>
 +
<p>Annually, about 5% to 15% of agricultural produce is lost due to frost. The formation of ice damages plants by rupturing cells and also through dehydration as water molecules are drawn out of the tissue. Current solutions to this problem - such as using heat or covering crops with protective material - are cumbersome, costly, and not fully preventative. Synthetic antifreeze chemicals have not been proven to work. Even if they did, they would be need to be applied repeatedly, at great cost, and may also leave residues in the environment.</p>
 +
</td> 
-
===Team===
+
</tr>
-
Tell us about your team, your school!
+
</table>
-
===Project===
+
<!--  Block of content 2 -->
-
What are you working on this semester?
+
<table width="100%"  cellspacing="0" height="500px">
 +
<tr>
 +
<td  bgColor="#FACA66"></td>
 +
<td width="975px" align="center" bgColor="#FACA66" >
 +
<table  width="975px"  cellspacing="0" height="500px">
 +
<tr>
 +
<td width="95%" bgColor="#FACA66"  rowspan="3" align="center">
-
===Notebook===
+
<!-- Content goes here -->
-
Show us how you spent your days.
+
<img src="https://static.igem.org/mediawiki/2014hs/c/cd/Plantifreeze.gif" width="500">
 +
<h3> Solution</h3>
 +
<p> The project undertaken by the Cambridge School of Weston (CSW) 2014 iGEM team aims to design a synthetic biology solution to this problem by building upon the work of the 2009 Utah State team that developed a protein secretion mechanism and that of the 2011 Yale team which synthesized an “antifreeze” protein (RiAFP) isolated from a cold-tolerant beetle called Rhagium inquisitor. This is the most potent antifreeze protein so far studied. Our device (Plantifreeze) is designed to mitigate crop damage caused by ice crystal formation on the plant surface. Plantifreeze is a dual plasmid system. <a href="https://2014hs.igem.org/Team:CSWProteens/project">Read more about our project.</a></p>
 +
</td>  </tr>
 +
</table>
-
===Results/Conclusions===
 
-
What did you achieve over the course of your semester?
 
-
===Safety===
 
-
What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?
 
-
===Attributions===
+
<!--  Block of content 3 -->
-
Who worked on what?
+
<table width="100%"  cellspacing="0" height="500px">
 +
<tr>
 +
<td  bgColor="#F7A541"></td>
 +
<td width="975px" align="center" bgColor="#F7A541" >
 +
<table  width="975px"  cellspacing="0" height="500px">
 +
<tr>
 +
<td width="95%" bgColor="#F7A541"  rowspan="3" align="center">
-
===Human Practices===
+
<!-- Content goes here -->
-
What impact does/will your project have on the public? 
+
 +
<a href="https://2014hs.igem.org/Team:CSWProteens/notebook"><img src="https://static.igem.org/mediawiki/2014hs/7/7a/Tumblr_myr5o6C8Fj1t12eweo2_1280.jpg" width="500">
 +
<h3> Follow our work in the lab.</h3></a>
-
===Fun!===
+
</td> 
-
What was your favorite team snack?? Have a picture of your team mascot?
+
</tr>
 +
</table>
-
<forum_subtle />
+
<!--  Block of content 4 -->
 +
<table width="100%"  cellspacing="0" height="500px">
 +
<tr>
 +
<td  bgColor="#F45D4C"></td>
 +
<td width="975px" align="center" bgColor="#F45D4C" >
 +
 
 +
<table  width="975px"  cellspacing="0" height="500px">
 +
<tr>
 +
<td width="95%" bgColor="#F45D4C"  rowspan="3" align="center">
 +
<!-- Content goes here -->
 +
 
 +
<a href="https://2014hs.igem.org/Team:CSWProteens"><img src="https://static.igem.org/mediawiki/2014hs/4/48/IMG_5239.jpg" width="500"></a>
 +
<h3>Human Practices</h3>
 +
<p> Although it has its roots in the long established discipline of molecular biology, the field of synthetic biology is still in its infancy. Consequently, the overwhelming majority of the public has no awareness of this emerging field. An informal pool of the student body at our own school in the Western suburbs of Boston (ground zero for synthetic biology) confirmed this notion. Consequently, the goal of our Human Practices effort was to enlighten the student body of our secondary school about synthetic biology and to promote interest in synthetic biology and iGEM to younger generations of students.</p>
 +
 
 +
</td>  </tr>
 +
</table>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<!-- Final block of content -->
 +
<table  id="firstblock" width="100%"  cellspacing="0" height="200px">
 +
<tr>
 +
<td  bgColor="#FFFFFF"></td>
 +
<td width="975px" align="center" bgColor="#FFFFFF" >
 +
 
 +
<p><a href="https://2014hs.igem.org/Team:CSWProteens/sponsors"><img src="https://static.igem.org/mediawiki/2014hs/6/66/Screen_Shot_2014-06-18_at_15.47.46.png"></a></p>
 +
 
 +
</td>
 +
<td bgColor="#FFFFFF"></td>
 +
</tr>
 +
</table>
 +
<!-- end of final block -->
 +
</table>
 +
</html>

Latest revision as of 02:14, 21 June 2014

Welcome!
We are the CSW Proteens!
Scroll down to find more about our project!

PlantiFreeze

Annually, about 5% to 15% of agricultural produce is lost due to frost. The formation of ice damages plants by rupturing cells and also through dehydration as water molecules are drawn out of the tissue. Current solutions to this problem - such as using heat or covering crops with protective material - are cumbersome, costly, and not fully preventative. Synthetic antifreeze chemicals have not been proven to work. Even if they did, they would be need to be applied repeatedly, at great cost, and may also leave residues in the environment.

Solution

The project undertaken by the Cambridge School of Weston (CSW) 2014 iGEM team aims to design a synthetic biology solution to this problem by building upon the work of the 2009 Utah State team that developed a protein secretion mechanism and that of the 2011 Yale team which synthesized an “antifreeze” protein (RiAFP) isolated from a cold-tolerant beetle called Rhagium inquisitor. This is the most potent antifreeze protein so far studied. Our device (Plantifreeze) is designed to mitigate crop damage caused by ice crystal formation on the plant surface. Plantifreeze is a dual plasmid system. Read more about our project.

Follow our work in the lab.

Human Practices

Although it has its roots in the long established discipline of molecular biology, the field of synthetic biology is still in its infancy. Consequently, the overwhelming majority of the public has no awareness of this emerging field. An informal pool of the student body at our own school in the Western suburbs of Boston (ground zero for synthetic biology) confirmed this notion. Consequently, the goal of our Human Practices effort was to enlighten the student body of our secondary school about synthetic biology and to promote interest in synthetic biology and iGEM to younger generations of students.