http://2014hs.igem.org/wiki/index.php?title=Team:CIDEB-UANL_Mexico/safety_riskanalysis&feed=atom&action=historyTeam:CIDEB-UANL Mexico/safety riskanalysis - Revision history2024-03-28T11:01:45ZRevision history for this page on the wikiMediaWiki 1.16.5http://2014hs.igem.org/wiki/index.php?title=Team:CIDEB-UANL_Mexico/safety_riskanalysis&diff=18872&oldid=prevDiegoValadez at 22:09, 18 June 20142014-06-18T22:09:07Z<p></p>
<a href="http://2014hs.igem.org/wiki/index.php?title=Team:CIDEB-UANL_Mexico/safety_riskanalysis&diff=18872&oldid=18698">Show changes</a>DiegoValadezhttp://2014hs.igem.org/wiki/index.php?title=Team:CIDEB-UANL_Mexico/safety_riskanalysis&diff=18698&oldid=prevPiff at 20:06, 18 June 20142014-06-18T20:06:04Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Performing genetic modifications in the project was not dangerous because the materials were handled carefully, in accordance with the “Laboratory Category One” guidelines published by the World Health Organization (WHO).</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Performing genetic modifications in the project was not dangerous because the materials were handled carefully, in accordance with the “Laboratory Category One” guidelines published by the World Health Organization (WHO).</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>The modified <i>E. coli</i> parts are safe for the environment and for human use. Variables are controlled by the team, as in the case of Capture module (NhaS) which is controlled by a UV promoter.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>The modified <i>E. coli</i> parts are safe for the environment and for human use. Variables are controlled by the team, as in the case of <ins class="diffchange diffchange-inline"><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_capture"></ins>Capture module<ins class="diffchange diffchange-inline"></a> </ins>(NhaS) which is controlled by a UV promoter.</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Similarly, the Aroma module is controlled by a Riboswitch regulated by temperature in order to decide when and how is going to be activated, besides adding salicylic acid, which is only harmful in high doses for humans, and in the project it is used in very small amounts (2mm) to activate it.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Similarly, the <ins class="diffchange diffchange-inline"><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_aroma"></ins>Aroma module<ins class="diffchange diffchange-inline"></a> </ins>is controlled by a Riboswitch regulated by temperature in order to decide when and how is going to be activated, besides adding salicylic acid, which is only harmful in high doses for humans, and in the project it is used in very small amounts (2mm) to activate it.</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>The <del class="diffchange diffchange-inline">resistance </del>module is also safe, since it gives the bacteria the ability to resist adverse conditions that would normally kill it, like high temperatures, high saline levels and high UV radiation, and nevertheless the bacteria would die in a normal environment.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>The <ins class="diffchange diffchange-inline"><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_resistance">Resistance </ins>module<ins class="diffchange diffchange-inline"></a> </ins>is also safe, since it gives the bacteria the ability to resist adverse conditions that would normally kill it, like high temperatures, high saline levels and high UV radiation, and nevertheless the bacteria would die in a normal environment.</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>The Union module gives E. CARU the ability to bind glass and silica surfaces, which we will use in beads form with the presentation that is used commercially. No direct risks are related to the module itself, but Silica gel beads may contain toxic and potentially carcinogenic cobalt (II) chloride, which is added as a moisture indicator in commercially available product. This point is analyzed further in the “Union as a safety measurement” section.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>The <ins class="diffchange diffchange-inline"><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_union"></ins>Union module<ins class="diffchange diffchange-inline"></a> </ins>gives E. CARU the ability to bind glass and silica surfaces, which we will use in beads form with the presentation that is used commercially. No direct risks are related to the module itself, but Silica gel beads may contain toxic and potentially carcinogenic cobalt (II) chloride, which is added as a moisture indicator in commercially available product. This point is analyzed further in the “Union as a safety measurement” section.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><b><h2>Union as a safety measurement</h2></b></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><b><h2>Union as a safety measurement</h2></b></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>The Union module in the project has a genetic device that gives the bacteria the ability to anchor itself to silica surfaces. The team used this ability to use the bacteria as a biological filter. This biological filter would clean the water free of <i>Escherichia coli</i> to meet a solution for salty water. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>The <ins class="diffchange diffchange-inline"><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_union"></ins>Union module<ins class="diffchange diffchange-inline"></a> </ins>in the project has a genetic device that gives the bacteria the ability to anchor itself to silica surfaces. The team used this ability to use the bacteria as a biological filter. This biological filter would clean the water free of <i>Escherichia coli</i> to meet a solution for salty water. </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Why silica pearls? Because silica pearls are very common to find, and even though that they are known for being harmful for humans if ingested, it’s not the silica gel that is toxic, what is toxic are the substances that the silica pearl can absorb (silica gel is known for its characteristic of having a great ability for absorbing humidity). Silica is, for example, used as a safe food additive, this means no permit is needed when added.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Why silica pearls? Because silica pearls are very common to find, and even though that they are known for being harmful for humans if ingested, it’s not the silica gel that is toxic, what is toxic are the substances that the silica pearl can absorb (silica gel is known for its characteristic of having a great ability for absorbing humidity). Silica is, for example, used as a safe food additive, this means no permit is needed when added.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>If people are not comfortable with using silica gel pearls for the biological filter, glass pearls can also be used, as the genetic device permits anchorage to glass surfaces, and glass has the advantage of not releasing any kind of substance if introduced in water, even for long periods of time, for the bacterium to attach. Also, for more efficiency in the de-contamination, glass recipients could be used, that way it would be easier for the anchorage of the bacterium.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>If people are not comfortable with using silica gel pearls for the biological filter, glass pearls can also be used, as the genetic device permits anchorage to glass surfaces, and glass has the advantage of not releasing any kind of substance if introduced in water, even for long periods of time, for the bacterium to attach. Also, for more efficiency in the de-contamination, glass recipients could be used, that way it would be easier for the anchorage of the bacterium.</p></div></td></tr>
</table>Piffhttp://2014hs.igem.org/wiki/index.php?title=Team:CIDEB-UANL_Mexico/safety_riskanalysis&diff=18694&oldid=prevPiff at 20:01, 18 June 20142014-06-18T20:01:59Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The use of reporters differing from the usual fluorescence proteins is one of the objectives iGEM CIDEB 2014 team is pursuing by using aromatic reporters, like banana or, in this case, wintergreen odor.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The use of reporters differing from the usual fluorescence proteins is one of the objectives iGEM CIDEB 2014 team is pursuing by using aromatic reporters, like banana or, in this case, wintergreen odor.</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>The <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_aroma">Aroma module<<del class="diffchange diffchange-inline">7a</del>> is used in order to prove the effectiveness of BSMT1 Opt CDS, for further use as an odor reporter for other teams and modules for this project. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>The <a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_aroma">Aroma module<<ins class="diffchange diffchange-inline">/a</ins>> is used in order to prove the effectiveness of BSMT1 Opt CDS, for further use as an odor reporter for other teams and modules for this project. </p></div></td></tr>
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</table>Piffhttp://2014hs.igem.org/wiki/index.php?title=Team:CIDEB-UANL_Mexico/safety_riskanalysis&diff=18692&oldid=prevPiff at 20:01, 18 June 20142014-06-18T20:01:06Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img width=270 height=220 src="https://static.igem.org/mediawiki/2014hs/1/16/WgCIDEB.jpg" align=left hspace=12></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img width=270 height=220 src="https://static.igem.org/mediawiki/2014hs/1/16/WgCIDEB.jpg" align=left hspace=12></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>This sequence will help to test its effectiveness and future usage as an odor reporter, since other teams (MIT 2006) have just analyzed it theoretically. IGEM CIDEB 2013 uses a riboswitch to induce the gene expression at high temperatures.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>This sequence will help to test its effectiveness and future usage as an odor reporter, since other teams (<ins class="diffchange diffchange-inline"><a href="https://2006.igem.org/wiki/index.php/MIT_2006"></ins>MIT 2006<ins class="diffchange diffchange-inline"></a></ins>) have just analyzed it theoretically. <ins class="diffchange diffchange-inline"><a href="https://2013hs.igem.org/Team:CIDEB-UANL_Mexico"></ins>IGEM CIDEB 2013<ins class="diffchange diffchange-inline"></a> </ins>uses a riboswitch to induce the gene expression at high temperatures.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>This piece (BSMT1 Opt) can replace RFP on capture module, or be added on union module; as wintergreen odor to demonstrate the presence of bacteria in silica beads or the capture of sodium ions on salty environments.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>This piece (BSMT1 Opt) can replace RFP on capture module, or be added on union module; as wintergreen odor to demonstrate the presence of bacteria in silica beads or the capture of sodium ions on salty environments.</p></div></td></tr>
</table>Piffhttp://2014hs.igem.org/wiki/index.php?title=Team:CIDEB-UANL_Mexico/safety_riskanalysis&diff=18688&oldid=prevPiff at 19:59, 18 June 20142014-06-18T19:59:29Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The use of reporters differing from the usual fluorescence proteins is one of the objectives iGEM CIDEB 2014 team is pursuing by using aromatic reporters, like banana or, in this case, wintergreen odor.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The use of reporters differing from the usual fluorescence proteins is one of the objectives iGEM CIDEB 2014 team is pursuing by using aromatic reporters, like banana or, in this case, wintergreen odor.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>The <del class="diffchange diffchange-inline">aroma </del>module is used in order to prove the effectiveness of BSMT1 Opt CDS, for further use as an odor reporter for other teams and modules for this project. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>The <ins class="diffchange diffchange-inline"><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_aroma">Aroma </ins>module<ins class="diffchange diffchange-inline"><7a> </ins>is used in order to prove the effectiveness of BSMT1 Opt CDS, for further use as an odor reporter for other teams and modules for this project. </p></div></td></tr>
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</table>Piffhttp://2014hs.igem.org/wiki/index.php?title=Team:CIDEB-UANL_Mexico/safety_riskanalysis&diff=18687&oldid=prevPiff at 19:58, 18 June 20142014-06-18T19:58:38Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>An UV Promoter (<a href="http://parts.igem.org/Part:BBa_I765001">BBa_I765001</a>) was chosen to begin the circuit in order to control the NhaS gene’s expression in E. CARU. The promoter is followed by (<a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>), the NhaS gene (<a href="http://parts.igem.org/Part:BBa_K1255000">BBa_K1255000</a>), then the same RBS, an RFP reporter (<a href="http://parts.igem.org/Part:BBa_E1010">BBa_E1010</a>) and a terminator (<a href="http://parts.igem.org/Part:BBa_B1002">BBa_B1002</a>).</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>An UV Promoter (<a href="http://parts.igem.org/Part:BBa_I765001">BBa_I765001</a>) was chosen to begin the circuit in order to control the NhaS gene’s expression in E. CARU. The promoter is followed by (<a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>), the NhaS gene (<a href="http://parts.igem.org/Part:BBa_K1255000">BBa_K1255000</a>), then the same RBS, an RFP reporter (<a href="http://parts.igem.org/Part:BBa_E1010">BBa_E1010</a>) and a terminator (<a href="http://parts.igem.org/Part:BBa_B1002">BBa_B1002</a>).</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Basically the same, just changing the RFP reporter for BSMT1 Opt (<a href="http://parts.igem.org/Part:BBa_K1255001">BBa_K1255001</a>), which is the CDS that is able to produce a Wintergreen aroma. For further information look at the Aroma module in this document.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Basically the same, just changing the RFP reporter for BSMT1 Opt (<a href="http://parts.igem.org/Part:BBa_K1255001">BBa_K1255001</a>), which is the CDS that is able to produce a Wintergreen aroma. For further information look at the <ins class="diffchange diffchange-inline"><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_aroma"></ins>Aroma module<ins class="diffchange diffchange-inline"></a> </ins>in this document.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><p><b>3. Aroma module</b><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><p><b>3. Aroma module</b><p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img width=130 height=210 src="https://static.igem.org/mediawiki/2014hs/8/88/BiofilterCIDEB.jpg" align=right hspace=12></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img width=130 height=210 src="https://static.igem.org/mediawiki/2014hs/8/88/BiofilterCIDEB.jpg" align=right hspace=12></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>This device is composed by a constitutive promoter (<a href="http://parts.igem.org/Part:BBa_J23119">BBa_J23119</a>), a common RBS (<a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>), a fusion protein that of a set which includes the CDS L2 with its peptide signal and AIDA, in order to make the protein for binding silica; a membrane protein L2 (<a href="http://parts.igem.org/Part:BBa_K888000">BBa_K888000</a>); AIDA (<a href="http://parts.igem.org/Part:BBa_K888001"><del class="diffchange diffchange-inline">BBa_888001</del></a>), and a terminator (<a href="http://parts.igem.org/Part:BBa_B1002">BBa_B1002</a>).</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>This device is composed by a constitutive promoter (<a href="http://parts.igem.org/Part:BBa_J23119">BBa_J23119</a>), a common RBS (<a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>), a fusion protein that of a set which includes the CDS L2 with its peptide signal and AIDA, in order to make the protein for binding silica; a membrane protein L2 (<a href="http://parts.igem.org/Part:BBa_K888000">BBa_K888000</a>); AIDA (<a href="http://parts.igem.org/Part:BBa_K888001"><ins class="diffchange diffchange-inline">BBa_K888001</ins></a>), and a terminator (<a href="http://parts.igem.org/Part:BBa_B1002">BBa_B1002</a>).</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>This module was available and proportionated by UANL iGEM 2013 team, however, iGEM CIDEB 2014 made some modifications to it, ligating the peptide signal and L2.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>This module was available and proportionated by UANL iGEM 2013 team, however, iGEM CIDEB 2014 made some modifications to it, ligating the peptide signal and L2.</p></div></td></tr>
</table>Piffhttp://2014hs.igem.org/wiki/index.php?title=Team:CIDEB-UANL_Mexico/safety_riskanalysis&diff=18677&oldid=prevPiff at 19:53, 18 June 20142014-06-18T19:53:02Z<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:53, 18 June 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 374:</td>
<td colspan="2" class="diff-lineno">Line 374:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img width=290 height=130 src="https://static.igem.org/mediawiki/2014hs/f/ff/ResistanceCIDEB.jpg" align=center hspace=12></center><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img width=290 height=130 src="https://static.igem.org/mediawiki/2014hs/f/ff/ResistanceCIDEB.jpg" align=center hspace=12></center><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>The sequence begins with a constitutive promoter (BBa_J23119), followed by an RBS (BBa_B0034), the gene IrrE (BBa_K729001) and a terminator (BBa_B1002).</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>The sequence begins with a constitutive promoter (<ins class="diffchange diffchange-inline"><a href="http://parts.igem.org/Part:</ins>BBa_J23119<ins class="diffchange diffchange-inline">">BBa_J23119</a></ins>), followed by an RBS (<ins class="diffchange diffchange-inline"><a href="http://parts.igem.org/Part:</ins>BBa_B0034<ins class="diffchange diffchange-inline">">BBa_B0034</a></ins>), the gene IrrE (<ins class="diffchange diffchange-inline"><a href="http://parts.igem.org/Part:</ins>BBa_K729001<ins class="diffchange diffchange-inline">">BBa_K729001</a></ins>) and a terminator (<ins class="diffchange diffchange-inline"><a href="http://parts.igem.org/Part:</ins>BBa_B1002<ins class="diffchange diffchange-inline">">BBa_B1002</a></ins>).</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><p><b>2. Capture module</b><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><p><b>2. Capture module</b><p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img width=310 height=130 src="https://static.igem.org/mediawiki/2014hs/8/82/ProjectCaptureCIDEB.jpg" align=center hspace=12></center><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img width=310 height=130 src="https://static.igem.org/mediawiki/2014hs/8/82/ProjectCaptureCIDEB.jpg" align=center hspace=12></center><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Since NhaS is putative, iGEM CIDEB 2014 decided to test the module with a red fluorescence protein <del class="diffchange diffchange-inline">(BBa_E1010)</del>, which is simpler than the original reporter idea for the module, and this allowed us to test one gene at a time in each module.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Since NhaS is putative, iGEM CIDEB 2014 decided to test the module with a red fluorescence protein, which is simpler than the original reporter idea for the module, and this allowed us to test one gene at a time in each module.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The sequence used for the NhaS experimentation is:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The sequence used for the NhaS experimentation is:</p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 390:</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img width=310 height=130 src="https://static.igem.org/mediawiki/2014hs/5/54/ExperimentCaptureCIDEB.jpg" align=center hspace=12></center><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img width=310 height=130 src="https://static.igem.org/mediawiki/2014hs/5/54/ExperimentCaptureCIDEB.jpg" align=center hspace=12></center><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>An UV Promoter (BBa_I765001) was chosen to begin the circuit in order to control the NhaS gene’s expression in E. CARU. The promoter is followed by <del class="diffchange diffchange-inline">an RBS </del>(BBa_B0034), the NhaS gene (BBa_K1255000), an RFP reporter (BBa_E1010) and a terminator (BBa_B1002).</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>An UV Promoter (<ins class="diffchange diffchange-inline"><a href="http://parts.igem.org/Part:</ins>BBa_I765001<ins class="diffchange diffchange-inline">">BBa_I765001</a></ins>) was chosen to begin the circuit in order to control the NhaS gene’s expression in E. CARU. The promoter is followed by <ins class="diffchange diffchange-inline"> </ins>(<ins class="diffchange diffchange-inline"><a href="http://parts.igem.org/Part:</ins>BBa_B0034<ins class="diffchange diffchange-inline">">BBa_B0034</a></ins>), the NhaS gene (<ins class="diffchange diffchange-inline"><a href="http://parts.igem.org/Part:</ins>BBa_K1255000<ins class="diffchange diffchange-inline">">BBa_K1255000</a></ins>)<ins class="diffchange diffchange-inline">, then the same RBS</ins>, an RFP reporter (<ins class="diffchange diffchange-inline"><a href="http://parts.igem.org/Part:</ins>BBa_E1010<ins class="diffchange diffchange-inline">">BBa_E1010</a></ins>) and a terminator (<ins class="diffchange diffchange-inline"><a href="http://parts.igem.org/Part:</ins>BBa_B1002<ins class="diffchange diffchange-inline">">BBa_B1002</a></ins>).</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Basically the same, just changing the RFP reporter for BSMT1 Opt (BBa_K1255001), which is the CDS that is able to produce a Wintergreen aroma. For further information look at the Aroma module in this document.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Basically the same, just changing the RFP reporter for BSMT1 Opt (<ins class="diffchange diffchange-inline"><a href="http://parts.igem.org/Part:</ins>BBa_K1255001<ins class="diffchange diffchange-inline">">BBa_K1255001</a></ins>), which is the CDS that is able to produce a Wintergreen aroma. For further information look at the Aroma module in this document.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><p><b>3. Aroma module</b><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><p><b>3. Aroma module</b><p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The use of reporters differing from the usual fluorescence proteins is one of the objectives iGEM CIDEB 2014 team is pursuing by using aromatic reporters, like banana or, in this case, wintergreen odor.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>The use of reporters differing from the usual fluorescence proteins is one of the objectives iGEM CIDEB 2014 team is pursuing by using aromatic reporters, like banana or, in this case, wintergreen odor.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>The aroma module is used in order to prove the effectiveness of BSMT1 Opt CDS <del class="diffchange diffchange-inline">(BBa_K1255001)</del>, for further use as an odor reporter for other teams and modules for this project. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>The aroma module is used in order to prove the effectiveness of BSMT1 Opt CDS, for further use as an odor reporter for other teams and modules for this project. </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img width=290 height=130 src="https://static.igem.org/mediawiki/2014hs/4/4c/AromaCIDEB.jpg" align=center hspace=12></center><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><img width=290 height=130 src="https://static.igem.org/mediawiki/2014hs/4/4c/AromaCIDEB.jpg" align=center hspace=12></center><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>BSMT1 (Salicylic Acid Carboxyl Methyltransferase I) is formed as part of a different circuit, composed by a constitutive promoter (BBa_J23119), a riboswitch (RNA thermometer, BBa_K115017), a CDS that, when it is induced by salicylic acid, it releases an enzymatic product (methyl salicylate), responsible of wintergreen odor, and a terminator (BBa_B1002).</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>BSMT1 (Salicylic Acid Carboxyl Methyltransferase I) is formed as part of a different circuit, composed by a constitutive promoter (<ins class="diffchange diffchange-inline"><a href="http://parts.igem.org/Part:</ins>BBa_J23119<ins class="diffchange diffchange-inline">">BBa_J23119</a></ins>), a riboswitch (RNA thermometer, <ins class="diffchange diffchange-inline"><a href="http://parts.igem.org/Part:</ins>BBa_K115017<ins class="diffchange diffchange-inline">">BBa_K115017</a></ins>), a CDS that, when it is induced by salicylic acid, it releases an enzymatic product (methyl salicylate), responsible of wintergreen odor <ins class="diffchange diffchange-inline">(<a href="http://parts.igem.org/Part:BBa_K1255001">BBa_K1255001</a>)</ins>, and a terminator (<ins class="diffchange diffchange-inline"><a href="http://parts.igem.org/Part:</ins>BBa_B1002<ins class="diffchange diffchange-inline">">BBa_B1002</a></ins>).</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img width=270 height=220 src="https://static.igem.org/mediawiki/2014hs/1/16/WgCIDEB.jpg" align=left hspace=12></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img width=270 height=220 src="https://static.igem.org/mediawiki/2014hs/1/16/WgCIDEB.jpg" align=left hspace=12></div></td></tr>
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<td colspan="2" class="diff-lineno">Line 419:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img width=130 height=210 src="https://static.igem.org/mediawiki/2014hs/8/88/BiofilterCIDEB.jpg" align=right hspace=12></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img width=130 height=210 src="https://static.igem.org/mediawiki/2014hs/8/88/BiofilterCIDEB.jpg" align=right hspace=12></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>This device is composed by a constitutive promoter (BBa_J23119), a common RBS (BBa_B0034), a fusion protein that of a set which includes the CDS L2 with its peptide signal and AIDA, in order to make the protein for binding silica; a membrane protein L2 (BBa_K888000); AIDA (BBa_888001), and a terminator (BBa_B1002).</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>This device is composed by a constitutive promoter (<ins class="diffchange diffchange-inline"><a href="http://parts.igem.org/Part:</ins>BBa_J23119<ins class="diffchange diffchange-inline">">BBa_J23119</a></ins>), a common RBS (<ins class="diffchange diffchange-inline"><a href="http://parts.igem.org/Part:</ins>BBa_B0034<ins class="diffchange diffchange-inline">">BBa_B0034</a></ins>), a fusion protein that of a set which includes the CDS L2 with its peptide signal and AIDA, in order to make the protein for binding silica; a membrane protein L2 (<ins class="diffchange diffchange-inline"><a href="http://parts.igem.org/Part:</ins>BBa_K888000<ins class="diffchange diffchange-inline">">BBa_K888000</a></ins>); AIDA (<ins class="diffchange diffchange-inline"><a href="http://parts.igem.org/Part:BBa_K888001"></ins>BBa_888001<ins class="diffchange diffchange-inline"></a></ins>), and a terminator (<ins class="diffchange diffchange-inline"><a href="http://parts.igem.org/Part:</ins>BBa_B1002<ins class="diffchange diffchange-inline">">BBa_B1002</a></ins>).</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>This module was available and proportionated by UANL iGEM 2013 team, however, iGEM CIDEB 2014 made some modifications to it, ligating the peptide signal and L2.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>This module was available and proportionated by UANL iGEM 2013 team, however, iGEM CIDEB 2014 made some modifications to it, ligating the peptide signal and L2.</p></div></td></tr>
</table>Piffhttp://2014hs.igem.org/wiki/index.php?title=Team:CIDEB-UANL_Mexico/safety_riskanalysis&diff=18661&oldid=prevPiff at 19:30, 18 June 20142014-06-18T19:30:47Z<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">Revision as of 19:30, 18 June 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><b><h2>Overview</h2></b></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><b><h2>Overview</h2></b></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align="justify">IGEM CIDEB 2014 considers biosafety as important as every other points of the iGEM competition. Because of this reason, the team decided to perform a Safety Risk Assessment focused on the project and in the lab practices needed to accomplish it. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align="justify">IGEM CIDEB 2014 considers biosafety as important as every other points of the iGEM competition. Because of this reason, the team decided to perform a <ins class="diffchange diffchange-inline"><a href="https://static.igem.org/mediawiki/2014hs/5/5f/SafetyRiskAnalysisCIDEB.pdf"></ins>Safety Risk Assessment<ins class="diffchange diffchange-inline"></a> </ins>focused on the project and in the lab practices needed to accomplish it. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In this assessment, a description of our host organism is made, along with the genetic modifications that were applied to it, including preventive measures to avoid its dissemination and appropriate identification and containment measures, in the case it was released into the environment. Also the overall potential risks of the project were included, taking in consideration all of the possible risks of working in our laboratory, along with preventive measures to reduce risk to a minimum.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In this assessment, a description of our host organism is made, along with the genetic modifications that were applied to it, including preventive measures to avoid its dissemination and appropriate identification and containment measures, in the case it was released into the environment. Also the overall potential risks of the project were included, taking in consideration all of the possible risks of working in our laboratory, along with preventive measures to reduce risk to a minimum.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Piffhttp://2014hs.igem.org/wiki/index.php?title=Team:CIDEB-UANL_Mexico/safety_riskanalysis&diff=18656&oldid=prevPiff at 19:26, 18 June 20142014-06-18T19:26:32Z<p></p>
<a href="http://2014hs.igem.org/wiki/index.php?title=Team:CIDEB-UANL_Mexico/safety_riskanalysis&diff=18656&oldid=18623">Show changes</a>Piffhttp://2014hs.igem.org/wiki/index.php?title=Team:CIDEB-UANL_Mexico/safety_riskanalysis&diff=18623&oldid=prevPiff at 18:58, 18 June 20142014-06-18T18:58:18Z<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 18:58, 18 June 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><font color="#0080FF"><p><b>Bibliography</b></font></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><font color="#0080FF"><p><b>Bibliography</b></font></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><font size="2"><p align=left>● ACETIC ACID (80%, 99.5%, Glacial). Hazard Communication Sheet. Retrieved May 12, 2014, from http://www.riskassessmentservices.co.uk/HazCom/Acetic%20Acid.pdf</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><font size="2"><p align=left>● ACETIC ACID (80%, 99.5%, Glacial). Hazard Communication Sheet. Retrieved May 12, 2014, from<ins class="diffchange diffchange-inline">: <a href="</ins>http://www.riskassessmentservices.co.uk/HazCom/Acetic%20Acid.pdf<ins class="diffchange diffchange-inline">">http://www.riskassessmentservices.co.uk/HazCom/Acetic%20Acid.pdf</a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● Agaroses Manual. Condalab. Retrieved May 12, 2014, from http://www.condalab.com/pdf/agarose_manual_09.pdf</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● Agaroses Manual. Condalab. Retrieved May 12, 2014, from<ins class="diffchange diffchange-inline">: <a href="</ins>http://www.condalab.com/pdf/agarose_manual_09.pdf<ins class="diffchange diffchange-inline">">http://www.condalab.com/pdf/agarose_manual_09.pdf</a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● <i> “A toxic death for ethidium bromide”</i>. (n.d.). Laboratory News. Retrieved May 13, 2014, from http://www.labnews.co.uk/features/a-toxic-death-for-ethidium-bromide/</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● <i> “A toxic death for ethidium bromide”</i>. (n.d.). Laboratory News. Retrieved May 13, 2014, from<ins class="diffchange diffchange-inline">: <a href="</ins>http://www.labnews.co.uk/features/a-toxic-death-for-ethidium-bromide/<ins class="diffchange diffchange-inline">">http://www.labnews.co.uk/features/a-toxic-death-for-ethidium-bromide/</a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● <i>Boric Acid. R.E.D. Facts. </i> Retrieved May 11, 2014, from http://www.epa.gov/oppsrrd1/REDs/factsheets/0024fact.pdf</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● <i>Boric Acid. R.E.D. Facts. </i> Retrieved May 11, 2014, from<ins class="diffchange diffchange-inline">: <a href="</ins>http://www.epa.gov/oppsrrd1/REDs/factsheets/0024fact.pdf<ins class="diffchange diffchange-inline">">http://www.epa.gov/oppsrrd1/REDs/factsheets/0024fact.pdf</a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● Calcium Chloride - MSDS. (n.d.). <i>Calcium Chloride - MSDS</i>. Retrieved May 13, 2014, <del class="diffchange diffchange-inline">fromhttp</del>://www.calciumchloride.co.uk/calcium_chloride_msds.html</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● Calcium Chloride - MSDS. (n.d.). <i>Calcium Chloride - MSDS</i>. Retrieved May 13, 2014, <ins class="diffchange diffchange-inline">from: <a href="http</ins>://www.calciumchloride.co.uk/calcium_chloride_msds.html<ins class="diffchange diffchange-inline">">http://www.calciumchloride.co.uk/calcium_chloride_msds.html</a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● CHEMWATCH. <i>Sodium Dodecyl Sulfate</i>. Santa Cruz Biotechnology, Inc. Retrieved May 12, 2014, from http://<del class="diffchange diffchange-inline">datasheets</del>.<del class="diffchange diffchange-inline">scbt</del>.<del class="diffchange diffchange-inline">com</del>/<del class="diffchange diffchange-inline">sc-264510</del>.<del class="diffchange diffchange-inline">pdf</del></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● CHEMWATCH. <i>Sodium Dodecyl Sulfate</i>. Santa Cruz Biotechnology, Inc. Retrieved May 12, 2014, from<ins class="diffchange diffchange-inline">: <a href="</ins>http://<ins class="diffchange diffchange-inline">www</ins>.<ins class="diffchange diffchange-inline">calciumchloride</ins>.<ins class="diffchange diffchange-inline">co.uk</ins>/<ins class="diffchange diffchange-inline">calcium_chloride_msds.html">http://www.calciumchloride.co.uk/calcium_chloride_msds.html</a></ins>.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● Curtiss, R, 1978, <i>Biological containment and cloning vector transmissibility, </i> J. Infectious Dis. 137:668-675</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● Curtiss, R, 1978, <i>Biological containment and cloning vector transmissibility, </i> J. Infectious Dis. 137:668-675<ins class="diffchange diffchange-inline">.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● Don Sarah (2008). <i>Optimal Conditions for the Growth of E Coli. </i> http://es.scribd.com/doc/11337868/Optimal-Conditions-for-the-Growth-of-E-<del class="diffchange diffchange-inline">ColiRetrieved May 11, 2014</del>. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● Don Sarah (2008). <i>Optimal Conditions for the Growth of E Coli. </i><ins class="diffchange diffchange-inline">Retrieved May 11, 2014, from: <a href="</ins>http://es.scribd.com/doc/11337868/Optimal-Conditions-for-the-Growth-of-E-<ins class="diffchange diffchange-inline">Coli">http://es.scribd.com/doc/11337868/Optimal-Conditions-for-the-Growth-of-E-Coli</a></ins>.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● EDETIC ACID (EDTA). <i>Summary Risk Assessment Report. </i> Retrieved May 12, 2014, from http://echa.europa.eu/documents/10162/5ed7db13-e932-4999-8514-378ce88ca51f</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● EDETIC ACID (EDTA). <i>Summary Risk Assessment Report. </i> Retrieved May 12, 2014, from<ins class="diffchange diffchange-inline">: <a href="</ins>http://echa.europa.eu/documents/10162/5ed7db13-e932-4999-8514-378ce88ca51f<ins class="diffchange diffchange-inline">">http://echa.europa.eu/documents/10162/5ed7db13-e932-4999-8514-378ce88ca51f</a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● <i>Environmental Health and Safety</i> (2007-09-10). "Silica Gel" http://hazard.com/msds/mf/baker/baker/files/s1610.htm. <del class="diffchange diffchange-inline">Retrieved 05-29-2014</del>. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● <i>Environmental Health and Safety</i> (2007-09-10). "Silica Gel<ins class="diffchange diffchange-inline">". Retrieved May 29, 2014, from: <a href=</ins>"http://hazard.com/msds/mf/baker/baker/files/s1610.htm<ins class="diffchange diffchange-inline">">http://hazard</ins>.<ins class="diffchange diffchange-inline">com/msds/mf/baker/baker/files/s1610.htm</a></ins>.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● Environmental Protection Agency. <i>Sodium Hydroxide. R.E.ED. Facts</i>. Retrieved May 1, 2014, <del class="diffchange diffchange-inline">fromhttp</del>://www.epa.gov/oppsrrd1/REDs/factsheets/4065fact.pdf</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● Environmental Protection Agency. <i>Sodium Hydroxide. R.E.ED. Facts</i>. Retrieved May 1, 2014, <ins class="diffchange diffchange-inline">from: <a href="http</ins>://www.epa.gov/oppsrrd1/REDs/factsheets/4065fact.pdf<ins class="diffchange diffchange-inline">">http://www.epa.gov/oppsrrd1/REDs/factsheets/4065fact.pdf</a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● Escherichia coli K-12 Derivatives <i>Final Risk Assessment. </i> EPA. Retrieved May 6, 2014, from http://epa.gov/biotech_rule/pubs/fra/fra004.htm</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● Escherichia coli K-12 Derivatives <i>Final Risk Assessment. </i> EPA. Retrieved May 6, 2014, from<ins class="diffchange diffchange-inline">: <a href="</ins>http://epa.gov/biotech_rule/pubs/fra/fra004.htm<ins class="diffchange diffchange-inline">">http://epa.gov/biotech_rule/pubs/fra/fra004.htm</a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● ETHANOL. <i>Hazard Communication Sheet</i>. Retrieved May 12, 2014, from http://www.riskassessmentservices.co.uk/HazCom/Ethanol.pdf</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● ETHANOL. <i>Hazard Communication Sheet</i>. Retrieved May 12, 2014, from<ins class="diffchange diffchange-inline">: <a href="</ins>http://www.riskassessmentservices.co.uk/HazCom/Ethanol.pdf<ins class="diffchange diffchange-inline">">http://www.riskassessmentservices.co.uk/HazCom/Ethanol.pdf</a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● Ethylenediaminetetraacetic acid. <i>Summary of Initial Risk Assessment Report. </i> Retrieved May 12, 2014, <del class="diffchange diffchange-inline">fromhttp</del>://www.safe.nite.go.jp/english/risk/pdf/03_summary/047sum.pdf</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● Ethylenediaminetetraacetic acid. <i>Summary of Initial Risk Assessment Report. </i> Retrieved May 12, 2014, <ins class="diffchange diffchange-inline">from: <a href="http</ins>://www.safe.nite.go.jp/english/risk/pdf/03_summary/047sum.pdf<ins class="diffchange diffchange-inline">">http://www.safe.nite.go.jp/english/risk/pdf/03_summary/047sum.pdf</a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● Fotadar U, Zaveloff P, Terracio L (2005). <i>"Growth of Escherichia coli at elevated temperatures"</i>. J. Basic Microbiology. 45 (5): 403–4.doi:10.1002/jobm.200410542. PMID 16187264 </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● Fotadar U, Zaveloff P, Terracio L (2005). <i>"Growth of Escherichia coli at elevated temperatures"</i>. J. Basic Microbiology. 45 (5): 403–4.doi:10.1002/jobm.200410542. PMID 16187264<ins class="diffchange diffchange-inline">.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● EPA (2006). <i>Ultraviolet disinfection guidance manual for the final long term 2 enhanced surface water treatment rule.</i> Retrieved May <del class="diffchange diffchange-inline">29th fromhttp</del>://www.epa.gov/ogwdw/disinfection/lt2/pdfs/guide_lt2_uvguidance.pdf</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● EPA (2006). <i>Ultraviolet disinfection guidance manual for the final long term 2 enhanced surface water treatment rule.</i> Retrieved May <ins class="diffchange diffchange-inline">29, 2014, from: <a href="http</ins>://www.epa.gov/ogwdw/disinfection/lt2/pdfs/guide_lt2_uvguidance.pdf<ins class="diffchange diffchange-inline">">http://www.epa.gov/ogwdw/disinfection/lt2/pdfs/guide_lt2_uvguidance.pdf</a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● EPA Guidance Manual (1999). <i>Alternative Disinfectants and Oxidants. </i> Chapter 8. Retrieved May <del class="diffchange diffchange-inline">29th </del>2014. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● EPA Guidance Manual (1999). <i>Alternative Disinfectants and Oxidants. </i> Chapter 8. Retrieved May <ins class="diffchange diffchange-inline">29, </ins>2014. </p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● <i>General Information Escherichia coli (E. coli) </i>. (2012, August 3). Centers for Disease Control and Prevention. Retrieved May 6, 2014, <del class="diffchange diffchange-inline">fromhttp</del>://www.cdc.gov/ecoli/general/index.html</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● <i>General Information Escherichia coli (E. coli) </i>. (2012, August 3). Centers for Disease Control and Prevention. Retrieved May 6, 2014, <ins class="diffchange diffchange-inline">from: <a href="http://www.cdc.gov/ecoli/general/index.html">http</ins>://www.cdc.gov/ecoli/general/index.html<ins class="diffchange diffchange-inline"></a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● UANL Mexico (2012). <i>Recovery module. </i> Retrieved on March <del class="diffchange diffchange-inline">28th</del>, from: https://2012.igem.org/Team:UANL_Mty-Mexico/Project/recovery</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● UANL Mexico (2012). <i>Recovery module. </i> Retrieved on March <ins class="diffchange diffchange-inline">28</ins>, from: <ins class="diffchange diffchange-inline"><a href="</ins>https://2012.igem.org/Team:UANL_Mty-Mexico/Project/recovery<ins class="diffchange diffchange-inline">">https://2012.igem.org/Team:UANL_Mty-Mexico/Project/recovery</a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● Helmestine Anne Marie, 2014. <i>What happens if you eat silica gel beads? </i> <del class="diffchange diffchange-inline">From </del>http://chemistry.about.com/od/medicalhealth/f/What-Happens-If-You-Eat-Silica-Gel-Beads.htm <del class="diffchange diffchange-inline">Retrieved May 16, 2014</del>. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● Helmestine Anne Marie, 2014. <i>What happens if you eat silica gel beads? </i> <ins class="diffchange diffchange-inline">Retrieved May 16, 2014, from: <a href="</ins>http://chemistry.about.com/od/medicalhealth/f/What-Happens-If-You-Eat-Silica-Gel-Beads.htm<ins class="diffchange diffchange-inline">">http://chemistry.about.com/od/medicalhealth/f/What-Happens-If-You-Eat-Silica-Gel-Beads.htm</a></ins>.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● IGEM biosafety 2013 from: http://www.biofaction.com/wp-content/uploads/2012/04/igem-biosafety-2013.pdf <del class="diffchange diffchange-inline">Retrieved May 16, 2014</del>. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● IGEM biosafety 2013<ins class="diffchange diffchange-inline">. Retrieved May 16, 2014 </ins>from: <ins class="diffchange diffchange-inline"><a href="</ins>http://www.biofaction.com/wp-content/uploads/2012/04/igem-biosafety-2013.pdf<ins class="diffchange diffchange-inline">">http://www.biofaction.com/wp-content/uploads/2012/04/igem-biosafety-2013.pdf</a></ins>.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● IVEY Mark, KRULWICH Terry. (1994) <i>Sodium ion binding proteins. </i> Retrieved April <del class="diffchange diffchange-inline">1st </del>2014 from http://www.google.com.mx/patents/US5346815</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● IVEY Mark, KRULWICH Terry. (1994) <i>Sodium ion binding proteins. </i> Retrieved April <ins class="diffchange diffchange-inline">1, </ins>2014 from<ins class="diffchange diffchange-inline">: <a href="</ins>http://www.google.com.mx/patents/US5346815<ins class="diffchange diffchange-inline">">http://www.google.com.mx/patents/US5346815</a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● Jin Huh (2008). <i>Part BBa_k112808</i>. <del class="diffchange diffchange-inline">From </del>https://2012.igem.org/Team:METU/KillSwitchOverview <del class="diffchange diffchange-inline">Retrieved May 16, 2014</del>. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● Jin Huh (2008). <i>Part BBa_k112808</i>. <ins class="diffchange diffchange-inline">Retrieved May 16, 2014, from: <a href="</ins>https://2012.igem.org/Team:METU/KillSwitchOverview<ins class="diffchange diffchange-inline">">https://2012.igem.org/Team:METU/KillSwitchOverview</a></ins>.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align=left>● Jones, T. F. (2007). <i>Investigation of Foodborne and Waterborne Disease Outbreaks. </i> In P. R. Murray, E. J. Baron, J. H. Jorgensen, M. L. Landry & M. A. Pfaller (Eds.), Manual of Clinical Microbiology (9th ed., pp. 152-169). Washignton, DC: ASM press. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p align=left>● Jones, T. F. (2007). <i>Investigation of Foodborne and Waterborne Disease Outbreaks. </i> In P. R. Murray, E. J. Baron, J. H. Jorgensen, M. L. Landry & M. A. Pfaller (Eds.), Manual of Clinical Microbiology (9th ed., pp. 152-169). Washignton, DC: ASM press. </p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● Kunhert, P, J Nicolet, and J Frey. "Applied and Environmental Microbiology." <i>Rapid and accurate identification of Escherichia Coli k-12 strands. </i> Ed. P Kunhert. N.P., 31 Aug. 1995. Web. <del class="diffchange diffchange-inline">13 </del>May 2014<del class="diffchange diffchange-inline">. </del><http://aem.asm.org/content/61/11/4135.full.pdf>.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● Kunhert, P, J Nicolet, and J Frey. "Applied and Environmental Microbiology." <i>Rapid and accurate identification of Escherichia Coli k-12 strands. </i> Ed. P Kunhert. N.P., 31 Aug. 1995. Web. <ins class="diffchange diffchange-inline">Retrieved </ins>May <ins class="diffchange diffchange-inline">13, </ins>2014<ins class="diffchange diffchange-inline">, from: </ins><<ins class="diffchange diffchange-inline">a href="</ins>http://aem.asm.org/content/61/11/4135.full.pdf<ins class="diffchange diffchange-inline">">http://aem.asm.org/content/61/11/4135.full.pdf</a</ins>>.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● McWilliams, M. <i>Luria Broth and Luria Agar Media and Their Uses Protocol</i>. American Society for Microbiology: Microbe Library. Retrieved May 12, 2014, from http://www.microbelibrary.org/component/resource/laboratory-test/3031-luria-broth-lb-and-luria-agar-la-media-and-their-uses-protocol</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● McWilliams, M. <i>Luria Broth and Luria Agar Media and Their Uses Protocol</i>. American Society for Microbiology: Microbe Library. Retrieved May 12, 2014, from<ins class="diffchange diffchange-inline">: <a href="</ins>http://www.microbelibrary.org/component/resource/laboratory-test/3031-luria-broth-lb-and-luria-agar-la-media-and-their-uses-protocol<ins class="diffchange diffchange-inline">">http://www.microbelibrary.org/component/resource/laboratory-test/3031-luria-broth-lb-and-luria-agar-la-media-and-their-uses-protocol</a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● <i>Material Safety Data Sheet</i>. United Biochemicals. Retrieved May 12, 2014, from http://www.unitedbiochemicals.com/msds/tris_hcl.pdf</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● <i>Material Safety Data Sheet</i>. United Biochemicals. Retrieved May 12, 2014, from<ins class="diffchange diffchange-inline">: <a href="</ins>http://www.unitedbiochemicals.com/msds/tris_hcl.pdf<ins class="diffchange diffchange-inline">">http://www.microbelibrary.org/component/resource/laboratory-test/3031-luria-broth-lb-and-luria-agar-la-media-and-their-uses-protocol</a>.</p></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● <i>Methyl salicylate overdose</i> Medline plus Medical Encyclopedia. (n.d.). U.S National Library of Medicine. Retrieved May 13, 2014, <del class="diffchange diffchange-inline">fromhttp</del>://www.nlm.nih.gov/medlineplus/ency/article/002683.htm</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● <i>Methyl salicylate overdose</i> Medline plus Medical Encyclopedia. (n.d.). U.S National Library of Medicine. Retrieved May 13, 2014, <ins class="diffchange diffchange-inline">from: <a href="http</ins>://www.nlm.nih.gov/medlineplus/ency/article/002683.htm<ins class="diffchange diffchange-inline">">http://www.microbelibrary.org/component/resource/laboratory-test/3031-luria-broth-lb-and-luria-agar-la-media-and-their-uses-protocol</a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● <i>MIT IGEM Team. </i> (2006). MIT 2006. Retrieved on March <del class="diffchange diffchange-inline">31th</del>, 2014<del class="diffchange diffchange-inline">. From</del>: https://2006.igem.org/wiki/index.php/MIT_2006</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● <i>MIT IGEM Team. </i> (2006). MIT 2006. Retrieved on March <ins class="diffchange diffchange-inline">31</ins>, 2014<ins class="diffchange diffchange-inline">, from; <a href="https</ins>:<ins class="diffchange diffchange-inline">//2006.igem.org/wiki/index.php/MIT_2006"></ins>https://2006.igem.org/wiki/index.php/MIT_2006<ins class="diffchange diffchange-inline"></a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● <i>Normas de Seguridad Laboratorio</i> <del class="diffchange diffchange-inline">From </del>http://www.uv.es/gammmm/Subsitio%20Operaciones/7%20normas%20de%20seguridad_archivos/NORMAS%20SEGURIDAD%20LABORATO RIO.pdf <del class="diffchange diffchange-inline">Retrieved May 16, 2014</del>. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● <i>Normas de Seguridad Laboratorio</i> <ins class="diffchange diffchange-inline">Retrieved May 16, 2014, from: <a href="</ins>http://www.uv.es/gammmm/Subsitio%20Operaciones/7%20normas%20de%20seguridad_archivos/NORMAS%20SEGURIDAD%20LABORATO RIO.pdf<ins class="diffchange diffchange-inline">">http://www.uv.es/gammmm/Subsitio%20Operaciones/7%20normas%20de%20seguridad_archivos/NORMAS%20SEGURIDAD%20LABORATO RIO.pdf</a></ins>.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● Pérez, E. (n.d.). <i>Boric acid poisoning</i>: Medline Plus Medical Encyclopedia. U.S National Library of Medicine. Retrieved May 12, 2014, <del class="diffchange diffchange-inline">fromhttp</del>://www.nlm.nih.gov/medlineplus/ency/article/002485.html</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● Pérez, E. (n.d.). <i>Boric acid poisoning</i>: Medline Plus Medical Encyclopedia. U.S National Library of Medicine. Retrieved May 12, 2014, <ins class="diffchange diffchange-inline">from: <a href="http</ins>://www.nlm.nih.gov/medlineplus/ency/article/002485.html<ins class="diffchange diffchange-inline">">http://www.nlm.nih.gov/medlineplus/ency/article/002485.html</a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● Ribonuclease A. (n.d.). - <i>Worthington Enzyme Manual. </i> Retrieved May 12, 2014, from http://www.worthington-biochem.com/RNASE/</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● Ribonuclease A. (n.d.). - <i>Worthington Enzyme Manual. </i> Retrieved May 12, 2014, from<ins class="diffchange diffchange-inline">: <a href="</ins>http://www.worthington-biochem.com/RNASE/<ins class="diffchange diffchange-inline">"></a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● Ribonuclease A. (n.d.). <i>RCSB PDB-101. </i> Retrieved May 12, 2014, from http://www.rcsb.org/pdb/101/motm.do?momID=105</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● Ribonuclease A. (n.d.). <i>RCSB PDB-101. </i> Retrieved May 12, 2014, from<ins class="diffchange diffchange-inline">: <a href="</ins>http://www.rcsb.org/pdb/101/motm.do?momID=105<ins class="diffchange diffchange-inline">">http://www.rcsb.org/pdb/101/motm.do?momID=105</a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● <i>"Silica Gel." </i> Centro De Información Toxicológica. CITUC. Red Salud UC Facultad De Medicina. Web. http://www.cituc.cl/temas/ficha1.php?id=<del class="diffchange diffchange-inline">22Retrieved 05-30-2014</del>. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● <i>"Silica Gel." </i> Centro De Información Toxicológica. CITUC. Red Salud UC Facultad De Medicina. Web. <ins class="diffchange diffchange-inline">Retrieved May 30, 2014, from: <a href="</ins>http://www.cituc.cl/temas/ficha1.php?id=<ins class="diffchange diffchange-inline">22">http://www.cituc.cl/temas/ficha1.php?id=22</a></ins>.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● <i>The known health effects of UV</i>. (n.d.). WHO. Retrieved May 13, 2014, from http://www.who.int/uv/faq/uvhealtfac/en/</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● <i>The known health effects of UV</i>. (n.d.). WHO. Retrieved May 13, 2014, from<ins class="diffchange diffchange-inline">: <a href="http://www.who.int/uv/faq/uvhealtfac/en/"></ins>http://www.who.int/uv/faq/uvhealtfac/en/<ins class="diffchange diffchange-inline"></a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● <i>Type 1 Water, Milli-Q and Ultrapure Water System</i>. (n.d.). Retrieved May 12, 2014, from http://www.millipore.com/lab_water/clw4/type1</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● <i>Type 1 Water, Milli-Q and Ultrapure Water System</i>. (n.d.). Retrieved May 12, 2014, from<ins class="diffchange diffchange-inline">: <a href="</ins>http://www.millipore.com/lab_water/clw4/type1<ins class="diffchange diffchange-inline">">http://www.millipore.com/lab_water/clw4/type1</a>.</ins></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● UCL iGEM Team. (2012). <i><del class="diffchange diffchange-inline">IrrE </del>module<del class="diffchange diffchange-inline">. </del></i> https://2012.igem.org/Team:University_College_London/Module_5. <del class="diffchange diffchange-inline">Retrieved March 31, 2014</del>. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● UCL iGEM Team. (2012). <i><ins class="diffchange diffchange-inline">irrE </ins>module</i><ins class="diffchange diffchange-inline">. Retrieved March 31, 2014, from: <a href="</ins>https://2012.igem.org/Team:University_College_London/Module_5<ins class="diffchange diffchange-inline">">https://2012</ins>.<ins class="diffchange diffchange-inline">igem.org/Team:University_College_London/Module_5</a></ins>.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p align=left>● Zubieta, Chole et al. (2003). <i>Structural Basis for Substrate Recognition in the Salicylic Acid Carboxyl Methyltransferase Family. </i> Manuscript submitted for publication. Retrieved from www.plantcell.org; American Society of Plant Biologists.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p align=left>● Zubieta, Chole et al. (2003). <i>Structural Basis for Substrate Recognition in the Salicylic Acid Carboxyl Methyltransferase Family. </i> Manuscript submitted for publication. Retrieved from <ins class="diffchange diffchange-inline"><a href="</ins>www.plantcell.org; American Society of Plant Biologists<ins class="diffchange diffchange-inline">">www.plantcell.org; American Society of Plant Biologists</a></ins>.</p></div></td></tr>
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