Team:CIDEB-UANL Mexico/project resistance

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iGEM CIDEB 2014 - Project

Resistance Module

Because our project is a biofilter which allows to remove salt from water, the bacteria needs to survive to extreme environmental conditions which normally it can’t do. We need E.Coli to survive a high salinity environment to allow it to capture Na ions and remove the salt concentration of the water.

In order to accomplish our project goal, we have to change E.coli metabolism and make it stronger, more resistant and more efficient than in normal E.coli bacteria.

Last discoveries show IrrE as a protein capable of change the E.coli metabolism, and giving it the ability to survive to bigger temperatures, bigger UV rays radiation and bigger salt concentration (UCL, 2012).

¿What does IrrE do?

The protein IrrE originates from Deinococcusradiodurans, and initially, this gene provides resistance to radiation. But when transformed in E. coli, it protects it against salt, oxidative and thermal shock. (UCL, 2012) Also, different experiments from different iGEM teams, support the idea of the bigger salt resistance in E. coli with this biobrick.

IMG_0317

Image 1. This graph done by UCL iGEM team 2012, shows how IrrE biobrick increased the salt concentration resistance in E. coli compared with the results from Tu Delf iGEM team 2010.

How it works?

IrrE has been demonstrated to up regulate transcription of recA and pprA - genes which encode Recombines A and Radiation Inducible Protein. With respect to salt tolerance, IrrE up regulates the production of several stress responsive proteins, protein kinases, metabolic proteins, and detoxification proteins. It also down regulates glycerol degradation. With this global regulatory effect, E. coli becomes more salt tolerant (UCL, 2012).

IMG_0317

Image 2. This diagram shows the effect of IrrE protein on E. coli metabolism.

Other teams that use it:

London 2012: They propose to confer salt tolerance on E. coli by linking the salt tolerance gene encoding the protein IrrE (BBa_K729001) to a constitutive promoter (BBa_J23119).

IMG_0317

Image 3. Part designed by UCL iGEM team 2012 for the irrE protein.

IrrEparts description :

IMG_0317

References

Huang, H. (2006, August 30). Part:BBa_B1002. . Retrieved August 30, 2014, from http://parts.igem.org/wiki/index.php?title=Part:BBa_B1002

Part:BBa_B0034. (2013, January 31). . Retrieved , from http://parts.igem.org/wiki/index.php?title=Part:BBa_B0034

Part:BBa_J23119. (2006, August 24). . Retrieved April 30, 2014, from http://parts.igem.org/wiki/index.php?title=Part:BBa_J23119

Sohrabi, B. (2012, June 27). Part:BBa_K729001. . Retrieved , from http://parts.igem.org/wiki/index.php?title=Part:BBa_K729001

UCLiGEM Team. (2012). irre module. Retrieved the 31, March of 2014 from https://2012.igem.org/Team:University_College_London/Module_5

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