Team:CIDEB-UANL Mexico/project abstract

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Description Capture Module Aroma Module Resistance Module Union Module Parts

iGEM CIDEB 2014 - Project

Abstract

iGEM CIDEB 2014 team’s project is a bio-filter of sodium ions in salt water. In order to work and test the different processes of the project, it was divided into four modules, which also made up the acronym of our project’s name: Capture, Aroma, Resistance, and Union. Capture module is in charge of recollecting sodium ions in the water due to the new NhaS gen registered by the team. Aroma module is with the help of salicylic acid, responsible of producing a Winter Green odor that acts as a reporter if the bio-filter is working. Resistance module allows the E. coli to survive in the salty environment of the water, and this allows the bio-filter to works. Finally, the Union module allow the bacterium to joins to a silica pearl, which facilitates the removal of the bacteria from the water.

Problem

Water always has been known as a source for life, but nowadays there is not enough usable water in the world. The global lack of abundance of usable water is an issue that presents a dangerous problem to our future. Ironically, only a small portion of our planet's water is actually usable. Ninety-seven percent of the world's water is too salty for consumption or agricultural use. Furthermore, much of the rest is held in ice caps or other unattainable sources. This leaves approximately one percent of the global water as liquid and fresh; ninety-eight percent of which is groundwater (Bouwer, 2002).

For solving this problem have been developed different methods. one of them is desalination, converting sea water (rich in salts) into usable water, but this method is very expensive by the great use of electrical energy and the extraction process produces dangerous wastes to the environment (Cotruvo, 2010).

For that reason our project is focused on developing a biological machine capable of performing desalination, reducing costs and avoiding dangerous wastes during the process. For making this possible, E. coli needed to capture Na+ ions in saline environments to be removed from water after performing its task.

Overview

Before E. coli could be able to remove Na+ ions form water, was needed to give it resistance to adverse conditions. This could be possible through a protein called IrrE which make E. coli resistant to saline environments as well as UV rays and temperature.

For giving the ability to capture Na+ ions was used the protein NhaS (a new part); able to bind and sequestering sodium ions. As a reporter for know if NhaS was expressed was used BSMT1 opt (optimized), a protein able to react with salicylic acid releasing a Wintergreen odor.

The final task E. coli should perform was to be able for being removed from the water after sequestering Na+ ions. In order to remove it, was used a fusion protein named L2+AIDA. L2 because gives the ability for binding silica to E. coli and AIDA as a tag for making L2 a membrane protein. With this ability E. coli could be removed from water through a biofilter made up of silica.

The complete circuit is shown in the figure 1. BSMT1 opt as a reporter for NhaS regulated by UV (for having a control of the NhaS expression), and IrrE with L2+AIDA continuously produced.

Figure 1. Diagram representing our proposed circuit

But we realized E. coli could have a genetic overload because the circuit was too big (approximately 5000 bp). Also the time we had to finish it was not enough, as well as most of the proteins we wanted to produce were putative or untested. So for a better understanding and for determine if each piece works we divided the project into four modules: capture, union, aroma and resistance, but the project is the result of their correlation. In fact our E. coli was named E. CARU (each letter by each module).

Escherichia coli

Project Zoom In

Bibliography

● Bouwer, H. (2002). Integrated Water Management for the 21st Century: Problems and Solutions. Journal of irrigation and drainage engineering, 193-200.

● Joseph Cotruvo, N. V. (2010). Desalination Technology: Health and Environmental Impacts. U.S: Taylor and Francis Group.

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iGEM CIDEB 2014 - Footer

iGEM CIDEB UANL 2014. CENTRO DE INVESTIGACIÓN Y DESARROLLO DE EDUCACIÓN BILINGÜE. UANL

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-{{:Team:CIDEB-UANL_Mexico/style2.css}}{{:Team:CIDEB-UANL_Mexico/menu_project}}<html xmlns="http://www.w3.org/1999/xhtml"><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><link href='http://fonts.googleapis.com/css?family=Oxygen:400,700' rel='stylesheet' type='text/css'><title>iGEM CIDEB 2014 - Project</title><style>body{margin:0;width:100%;padding:0;background:#2056ac;font-family:'Oxygen',sans-serif;font-size:12pt;background-image:url(https://static.igem.org/mediawiki/2014hs/3/32/Cideb_Fondo1.gif)}h1,h2,h3{margin:0;padding-bottom:5px;color:#404040}p,ol,ul{margin-top:0}ol,ul{padding:0;list-style:none}p{line-height:1.60em;padding- right:3em}a{color:#2056ac}a:hover{text-decoration:none}.container{margin:0 auto;width:1200px}.container-text{margin:0 auto;width:75%;padding:0;font-family:'Oxygen',sans-serif;font-size:12pt;text-align:justify}.wrapper{overflow:hidden;padding:0 0 1em 0;background:#FFF}#wrapper1{background:#FFF}#wrapper2{overflow:hidden;background:#f3f3f3;padding:5em 0;text-align:center}#wrapper3{overflow:hidden;padding:0;background:#FFF}#banner{padding-top:2em}#welcome{overflow:hidden;width:1000px;padding:0 100px 0 100px;text-align:center}#welcome .content{padding:0 8em}.title{margin-bottom:1em}.title h2{font-size:2em}.title .byline{font-size:1.1em;color:#6F6F6F#}#three-column{overflow:hidden;margin-top:5em;padding-top:1em;border-top:1px solid rgba(0,0,0,0.2);text-align:center}#three-column h2{margin:1em 0;font-size:1.5em;font-weight:700}#three-column .icon{position:relative;display:block;margin:0 auto .80em auto;background:0;line-height:150px;font-size:4em;width:150px;height:100px;border-radius:100px;border:6px solid #67128f;text-align:center;color:#FFF}#three-column #tbox1,#three-column #tbox2,#three-column #tbox3{float:left;width:320px;padding:30px 40px 50px 40px}#three-column .title{text-align:center}#three-column .title h2{font-size:1.60em}#three-column .title .byline{padding-top:.50em;font-size:.90em;color:#858585}#three-column .arrow-down{border-top-color:#292929}ul.tools{margin:0;padding:0;list-style:none}ul.tools li{display:inline-block;padding:0 .2em;font-size:4em}ul.tools li span{display:none;margin:0;padding:0}ul.tools li a{color:#FFF}ul.tools li a:before{display:inline-block;background:#1abc9c;width:120px;height:120px;border-radius:50%;line-height:120px;text-align:center;color:#fff}.button{display:inline-block;margin-top:2em;padding:.8em 2em;background:#64abd1;line-height:1.8em;letter-spacing:1px;text-decoration:none;font-size:1em;color:#FFF}.button:before{display:inline-block;background:#8dcb89;margin-right:1em;width:40px;height:40px;line-height:40px;border-radius:20px;text-align:center;color:#272925}#portfolio{overflow:hidden;padding-top:5em;border-top:1px solid rgba(0,0,0,0.2)}#portfolio .box{text-align:center;color:rgba(0,0,0,0.5)}#portfolio h3{display:block;padding-bottom:1em;font-size:1em;color:rgba(0,0,0,0.6)}#portfolio .title{text-align:center}#portfolio .title h2{color:rgba(0,0,0,0.8)}.column1,.column2,.column3,.column4{width:282px}.column1,.column2,.column3{float:left;margin-right:24px}.column4{float:right}</style><body><div class="wrapper"><div id="welcome" class="container"><div class="title"><h2>Abstract</h2></div></div><div class="container-text"><table width=100%><tr><td><p>Since always, water has been known as a source for life. We cannot survive without water. Although useful water is a necessity which must be satisfied for everyone, nowadays in certain countries there are no enough water supplies for people. The global lack of abundance of usable water is an issue that presents a dangerous problem to our future. Ironically, only a small portion of our planet's water is actually usable. Ninety-seven percent of the world's water is too salty for consumption or agricultural use. Furthermore, much of the rest is held in ice caps or other unattainable sources. This leaves approximately one percent of the global water as liquid and fresh; ninety-eight percent of which is groundwater (Bouwer, 2002).</p></td><td style="padding-left:12px"><img width=265 height=243 src="https://static.igem.org/mediawiki/2014hs/archive/c/c9/20140609211415%21Logo.png"/></td></tr></table><p>In fact, for solving this problem have been developed different methods. One of them is desalination, converting sea water (rich in salts) into usable water; but this method is very expensive by the great use of electrical energy, and the extraction process produces wastes dangerous for the environment (Cotruvo, 2010).</p><p>For that reason our project is focused on developing a biological machine capable of performing desalination, reducing costs and avoiding dangerous wastes during the process. For making this possible, <i>E. coli</i> must survive in saline environments, able to capture salts, and be removed from the water after the process. In order to achieve the objective we designed a biological circuit in which <i>E. coli </i>could be able to resist adverse conditions though a protein called IrrE, capture Na<SUP>+</SUP> ions (this because sodium chloride is the main salt of sea water) by NhaS production releasing an aroma (BSMT1 opt.) as reporter, and be able for binding silica (L2+AIDA) in order to remove it through a biofilter. The whole circuit is shown below:</p><center><p><img width=552 height=343 src="https://static.igem.org/mediawiki/2014hs/c/c4/Circuito_e.caru.png" align=center hspace=12 alt="IMG_0317"></p><p><b>Figure 1.</b> Diagram representing our proposed circuit</p></center><p>But we realized <i>E. coli</i> could have a genetic overload because the circuit was too big (approximately 5000 bp). Also the time we had to finish it was not enough, as well as most of the proteins we wanted to produce were putative or untested. So for a better understanding and for determine if each piece works we divided the project into four modules: capture, binding, aroma and resistance, but the project is the result of their correlation. In fact our bacteria was named E. CARU (each letter by each module). </p><center><table width=60%><tr><td><p><b>E</b>scherichia coli</p><p><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_capture"><b>C</b>apture</a></p><p><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_aroma"><b>A</b>roma</a></p><p><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_resistance"><b>R</b>esistance </a></p><p><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_union"><b>U</b>nion</a></p></p></td><td style="padding-left:12px"><img width=286 height=285 src="https://static.igem.org/mediawiki/2014hs/3/30/Image006cideb2014.png"/></td></tr></table></center><p><b>Future results</b></p><p>Once we have proved each piece works alone, and we obtained experimental data to support their effectiveness we planned to join every module into the whole circuit we propose at first. It would mean to place IrrE and L2+AIDA gene in <i>E. coli</i>. In the case of NhaS and Wintergreen we would replace the RFP gene from NhaS with the Wintergreen reporter.</p><p><h2><b>Project Zoom In</b></h2></p></br><center><iframe width="600" height="500" src="//www.youtube.com/embed/CK3AVE8UfWU" frameborder="0" allowfullscreen></iframe></center><p><b><h2>Bibliography</h2></b></p><font size="2"><p>● Bouwer, H. (2002). Integrated Water Management for the 21st Century: Problems and Solutions. Journal of irrigation and drainage engineering, 193-200.</p><p>● Joseph Cotruvo, N. V. (2010). Desalination Technology: Health and Environmental Impacts. U.S: Taylor and Francis Group.</p><br><div style="text-align:right"><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_abstract#"><font color="blue">Return to the Top</font></a></p></div></div></div></body></html>{{:Team:CIDEB-UANL_Mexico/footer}}
+{{:Team:CIDEB-UANL_Mexico/style2.css}}
+{{:Team:CIDEB-UANL_Mexico/menu_project}}
+<html xmlns="http://www.w3.org/1999/xhtml">
+<head>
+<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
+<link href='http://fonts.googleapis.com/css?family=Oxygen:400,700' rel='stylesheet' type='text/css'>
+<title>iGEM CIDEB 2014 - Project</title>
+<style>body{margin:0;width:100%;padding:0;background:#2056ac;font-family:'Oxygen',sans-serif;font-size:12pt;background-image:url(https://static.igem.org/mediawiki/2014hs/3/32/Cideb_Fondo1.gif)}h1,h2,h3{margin:0;padding-bottom:5px;color:#404040}p,ol,ul{margin-top:0}ol,ul{padding:0;list-style:none}p{line-height:1.60em;padding- right:3em}a{color:#2056ac}a:hover{text-decoration:none}.container{margin:0 auto;width:1200px}.container-text{margin:0 auto;width:75%;padding:0;font-family:'Oxygen',sans-serif;font-size:12pt;text-align:justify}.wrapper{overflow:hidden;padding:0 0 1em 0;background:#FFF}#wrapper1{background:#FFF}#wrapper2{overflow:hidden;background:#f3f3f3;padding:5em 0;text-align:center}#wrapper3{overflow:hidden;padding:0;background:#FFF}#banner{padding-top:2em}#welcome{overflow:hidden;width:1000px;padding:0 100px 0 100px;text-align:center}#welcome .content{padding:0 8em}.title{margin-bottom:1em}.title h2{font-size:2em}.title .byline{font-size:1.1em;color:#6F6F6F#}#three-column{overflow:hidden;margin-top:5em;padding-top:1em;border-top:1px solid rgba(0,0,0,0.2);text-align:center}#three-column h2{margin:1em 0;font-size:1.5em;font-weight:700}#three-column .icon{position:relative;display:block;margin:0 auto .80em auto;background:0;line-height:150px;font-size:4em;width:150px;height:100px;border-radius:100px;border:6px solid #67128f;text-align:center;color:#FFF}#three-column #tbox1,#three-column #tbox2,#three-column #tbox3{float:left;width:320px;padding:30px 40px 50px 40px}#three-column .title{text-align:center}#three-column .title h2{font-size:1.60em}#three-column .title .byline{padding-top:.50em;font-size:.90em;color:#858585}#three-column .arrow-down{border-top-color:#292929}ul.tools{margin:0;padding:0;list-style:none}ul.tools li{display:inline-block;padding:0 .2em;font-size:4em}ul.tools li span{display:none;margin:0;padding:0}ul.tools li a{color:#FFF}ul.tools li a:before{display:inline-block;background:#1abc9c;width:120px;height:120px;border-radius:50%;line-height:120px;text-align:center;color:#fff}.button{display:inline-block;margin-top:2em;padding:.8em 2em;background:#64abd1;line-height:1.8em;letter-spacing:1px;text-decoration:none;font-size:1em;color:#FFF}.button:before{display:inline-block;background:#8dcb89;margin-right:1em;width:40px;height:40px;line-height:40px;border-radius:20px;text-align:center;color:#272925}#portfolio{overflow:hidden;padding-top:5em;border-top:1px solid rgba(0,0,0,0.2)}#portfolio .box{text-align:center;color:rgba(0,0,0,0.5)}#portfolio h3{display:block;padding-bottom:1em;font-size:1em;color:rgba(0,0,0,0.6)}#portfolio .title{text-align:center}#portfolio .title h2{color:rgba(0,0,0,0.8)}.column1,.column2,.column3,.column4{width:282px}.column1,.column2,.column3{float:left;margin-right:24px}.column4{float:right}</style>
+<body>
+<div class="wrapper">
+<div id="welcome" class="container">
+   <div class="title">
+      <h2>Abstract</h2>
+   </div>
+</div>
+<div class="container-text">
+   <p>iGEM CIDEB 2014 team’s project is a bio-filter of sodium ions in salt water. In order to work and test the different processes of the project, it was divided into four modules, which also made up the acronym of our project’s name: Capture, Aroma, Resistance, and Union. Capture module is in charge of recollecting sodium ions in the water due to the new NhaS gen registered by the team. Aroma module is with the help of salicylic acid, responsible of producing a Winter Green odor that acts as a reporter if the bio-filter is working. Resistance module allows the <i>E. coli</i> to survive in the salty environment of the water, and this allows the bio-filter to works. Finally, the Union module allow the bacterium to joins to a silica pearl, which facilitates the removal of the bacteria from the water.</p>
+</div>
+<div id="welcome" class="container">
+   <div class="title">
+      <h2>Problem</h2>
+   </div>
+</div>
+<div class="container-text">
+   <table width=100%>
+      <tr>
+         <td>
+            <p>Water always has been known as a source for life, but nowadays there is not enough usable water in the world. The global lack of abundance of usable water is an issue that presents a dangerous problem to our future. Ironically, only a small portion of our planet's water is actually usable. Ninety-seven percent of the world's water is too salty for consumption or agricultural use. Furthermore, much of the rest is held in ice caps or other unattainable sources. This leaves approximately one percent of the global water as liquid and fresh; ninety-eight percent of which is groundwater (Bouwer, 2002).</p>
+         </td>
+         <td style="padding-left:12px">
+            <img width=265 height=243 src="https://static.igem.org/mediawiki/2014hs/archive/c/c9/20140609211415%21Logo.png"/>
+         </td>
+      </tr>
+   </table>
+   <p>For solving this problem have been developed different methods. one of them is desalination, converting sea water (rich in salts) into usable water, but this method is very expensive by the great use of electrical energy and the extraction process produces dangerous wastes to the environment (Cotruvo, 2010).</p>
+   <p>For that reason our project is focused on developing a biological machine capable of performing desalination, reducing costs and avoiding dangerous wastes during the process. For making this possible, E. coli needed to capture Na+ ions in saline environments to be removed from water after performing its task.</p>
+</div>
+<div id="welcome" class="container">
+   <div class="title">
+      <h2>Overview</h2>
+   </div>
+</div>
+<div class="container-text">
+   <p>Before <i>E. coli</i> could be able to remove Na+ ions form water, was needed to give it resistance to adverse conditions. This could be possible through a protein called IrrE which make <i>E. coli</i> resistant to saline environments as well as UV rays and temperature.</p>
+   <p>For giving the ability to capture Na+ ions was used the protein NhaS (a new part); able to bind and sequestering sodium ions. As a reporter for know if NhaS was expressed was used BSMT1 opt (optimized), a protein able to react with salicylic acid releasing a Wintergreen odor.</p>
+   <p>The final task <i>E. coli</i> should perform was to be able for being removed from the water after sequestering Na+ ions. In order to remove it, was used a fusion protein named L2+AIDA. L2 because gives the ability for binding silica to <i>E. coli</i> and AIDA as a tag for making L2 a membrane protein. With this ability <i>E. coli</i> could be removed from water through a biofilter made up of silica.</p>
+   <p>The complete circuit is shown in the <b>figure 1</b>. BSMT1 opt as a reporter for NhaS regulated by UV (for having a control of the NhaS expression), and IrrE with L2+AIDA continuously produced.</p>
+   <center>
+      <p><img width=552 height=343 src="https://static.igem.org/mediawiki/2014hs/6/6a/Correct_circuit_cideb.png" align=center hspace=12 alt="IMG_0317"></p>
+      <p><b>Figure 1.</b> Diagram representing our proposed circuit</p>
+   </center>
+   <p>But we realized <i>E. coli</i> could have a genetic overload because the circuit was too big (approximately 5000 bp). Also the time we had to finish it was not enough, as well as most of the proteins we wanted to produce were putative or untested. So for a better understanding and for determine if each piece works we divided the project into four modules: capture, union, aroma and resistance, but the project is the result of their correlation. In fact our <i>E. coli</i> was named E. CARU (each letter by each module). </p>
+   <center>
+      <table width=60%>
+         <tr>
+            <td>
+               <p><b>E</b>scherichia coli</p>
+               <p><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_capture"><b>C</b>apture</a></p>
+               <p><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_aroma"><b>A</b>roma</a></p>
+               <p><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_resistance"><b>R</b>esistance </a></p>
+               <p><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_union"><b>U</b>nion</a></p>
+            </td>
+            <td style="padding-left:12px"><img width=286 height=285 src="https://static.igem.org/mediawiki/2014hs/3/30/Image006cideb2014.png"/>
+            </td>
+         </tr>
+      </table>
+   </center>
+   <p><h2><b>Project Zoom In</b></h2></p></br>
+   <center><iframe width="600" height="500" src="//www.youtube.com/embed/CK3AVE8UfWU" frameborder="0" allowfullscreen></iframe>
+   </center>
+   <p><b><h2>Bibliography</h2></b></p>
+   <font size="2">
+      <p>● Bouwer, H. (2002). Integrated Water Management for the 21st Century: Problems and Solutions. Journal of irrigation and drainage engineering, 193-200.</p>
+      <p>● Joseph Cotruvo, N. V. (2010). Desalination Technology: Health and Environmental Impacts. U.S: Taylor and Francis Group.</p><br>
+   <div style="text-align:right"><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/project_abstract#"><font color="blue">Return to the Top</font></a></p></div>
+</div>
+</div>
+</body>
+</html>
+{{:Team:CIDEB-UANL_Mexico/footer}}