Team:CIDEB-UANL Mexico/math aroma

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iGEM CIDEB 2014 - Project

Aroma Module

Aroma module is based in the production of SAM/salicylic acid methyltransferase (BSMT1) in order to generate methyl salicylate, leaving a physical evidence of wintergreen odor. As this module is not affected by external factors during its transcription, it was used the established formula of mRNA and their parameters but with the data we obtained from BSMT1.


\begin{equation} \large \frac{d\left [ mRNA \right ]}{dt}=\alpha_{1}-d_{1}\left [ mRNA \right ] \end{equation}

In the case of translation, a ribo-switch (RNA thermometer) affects the production of the protein BSMT1. When the temperature reaches 32°C, ribo-switch allows the translation of BSMT1 gene, but at less of it (<32°C), translation is not allowed.


\begin{equation} \large RBS\left\{\begin{matrix} T<32=0 & \\ T\geq 32=1& \end{matrix}\right. \end{equation}
\begin{equation} \large \frac{d[P]}{dt} = \alpha_{2} \cdot[mRNA] - d_{2}[P] - f_{post}\left\{\begin{matrix} T<32 \left\{\begin{matrix} \alpha_{2}=\frac{2400\cdot 0}{358}; R=0 & \\ No protein degradation& \end{matrix}\right. & \\ T\geq 32 \left\{\begin{matrix} \alpha_{2}\frac{2400}{358}=6.7& \\ d_{1}\frac{1}{40min}+ \frac{1}{30min}=0.058 & \end{matrix}\right.& \end{matrix}\right. \end{equation}

We used the parameters for translation and transcription rate from Singapore 2008 iGEM team as well as the speeds at which E. coli carry out transcription and translation assuming a transcription speed of 70nt/s and a translation speed of 40aa/s. So we used them in the equations below with the Wintergreen gene length (1198nt) and protein length (358aa) respectively.


\begin{equation} \large \alpha_{1} = \frac{transcription speed}{gene length \cdot(nt)} \end{equation}
\begin{equation} \large \alpha_{2} = \frac{translation speed}{protein length \cdot(aa)} \end{equation}
\begin{equation} \large \alpha_{1} = \frac{(70)(60)}{1198} = 3.5 \end{equation}
\begin{equation} \large \alpha_{2} = \frac{(40)(60)}{358} = 6.7 \end{equation}

Then, we used the parameters for degradation rates for proteins and mRNAs from Beijing PKU 2009 iGEM team:


\begin{equation} \large d_{1} = \frac{1}{half-life} + \frac{1}{30} \cdot(min) \end{equation}
\begin{equation} \large d_{2} = \frac{1}{half-life} + \frac{1}{30} \cdot(min) \end{equation}

Since the half-life of BSMT1 has not been determined by MIT Team 2006, we searched it and according to Zubieta (2003), the average half-life for salicylic acid methyltransferases are about 40min.

For determining the degradation rate of average mRNA we used the information from Selinger’s team (2003). They carried several experiments for finding average mRNA half-life in E. coli. They used mRNAs about 1100nt concluding they have an average half-life of 5min. So with this we found the average mRNA half-life of BSMT1 was 5.44min.


\begin{equation} \large HL = \frac{1100 \cdot(bp)}{5 min} \end{equation}

With all these information we could find the degradation rates for both transcription and translation of BSMT1.


\begin{equation} \large d_{1} = \frac{1}{5.44} + \frac{1}{30} = 0.21 \end{equation}
\begin{equation} \large d_{2} = \frac{1}{40} + \frac{1}{30} = 0.058 \end{equation}

For the simulation we used Simbiology using the previous data in the equations for finding the amount of proteins E. coli would produce at certain time. The following was the result of the simulation.


IMG_0317

But for translation there was another factor we had to consider, the “fpost which were the post-translational variables affecting the production of the functional protein.


\begin{equation} \large \frac{d[P]}{dt} = \alpha_{2} \cdot[mRNA] - d_{2}[P] - f_{post} \end{equation}

During the researching of information, we found that BSMT1 is a special type of enzyme called Michaelis-Menten enzyme. As BSMT1 will perform an enzymatic reaction, it was needed to know at which rate it will be carried on producing methyl salicylate (Zubieta 2003). For that reason, the post-translational function considers the rate of methyl salicylate production as the variable that directly affects the production of the final protein. The formula used was also called Michaelis-Menten Equation:


\begin{equation} \large f_{post}=\frac{k_{cat}\left [ S \right ]\left [ E \right ]}{K_{m} + \left [ S \right ]} \end{equation}

Where: “[S]” means the substrate concentration; “[E]”, the enzyme concentration (obtained by the rest of the translational formula); “Kcat; is the turnover number; and “Km, the substrate concentration needed to achieve a half-maximum enzyme velocity.

Zubieta et al. (2003) established “Km and “Kcat values for SAM (BSMT1) of 23 and 0.092, respectively. All this values are used when a salicylic acid concentration of 2mM is induced. As “[E]” stands for enzyme concentration, the protein produced (by the rates of translation and degradation of the protein) will introduce this value in the equation. By substituting these values in the Michaelis-Menten Equation, it will change as follows:


\begin{equation} \large f_{post}=\frac{k_{cat}\left [ S \right ]\left [ E \right ]}{K_{m} + \left [ S \right ]}=\frac{0.092\left ( 2mM \right )\left [ P \right ]}{23 + 2mM} \end{equation}

Also, this formula is used in order to get the maximum rate of methyl salicylate production. This value is given by the product of “Kcat times the substrate concentration “[S]”.


\begin{equation} \large V_{max}=K_{cat}\cdot \left [ S \right ]=0.092\left ( 2mM \right )=0.184 \end{equation}

We used the enzymatic rate in Simbiology for modelling the functional BSMT1 production. The results were the following:

IMG_0317

IMG_0317


With the analysis from both graphs (Graph 1 and Graph 2) , we realized that the enzymatic reaction was too slow compared with the BSMT1 production, leaving almost ¼ nonfunctional of the total amount of proteins produced. We assumed that the functional BSMT1 is the final product, which releases the Wintergreen odor and it would be relatively concentrated according with the amount of proteins produced.


Bibliography

● David Dibden, J. G. (2005). In vivo cycling of the Escherichia coli transcription factor FNR between active and inactive states. Microbiology, 4063-4070.

● Douglas Selinger, R. M. (2003). Global RNA Half-Life Analysis in Escherichia coli Reveals Positional Patterns of Transcript Degradation. Genome Research, 216-223.

● Zubieta, C. (2003). Structural Basis for Substrate Recognition in the Salicylic Acid Carboxyl Methyltransferase Family. Plant Cell, 1704-1716.


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