Team:CIDEB-UANL Mexico/math aroma

Aroma modules is based in the production of SAM/salicylic acid methyltransferase (BSMT1), to produce methyl salicylate, leaving a physical evidence of wintergreen odor. As this module is not affected by external factors during its transcription, it was used the stablished formula of mRNA and their parameters but with the data we obtained from BSMT1.

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In the case of transalation, a ribo-switch (RNA thermometer) affects the production of the protein BSMT1 (Wintergreen). When the temperature reaches 32°C, ribo-switch allows the transaltion of BSMT1 gene, but at less of it (<32°C), transaltion is not allowed.

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We used the parameters for translation and transcription rate from Singapore 2008 iGEM team as well as the speeds at which E. coli carry out transcription and translation assuming a transcription speed of 70nt/s and a translation speed of 40aa/s. So we used them in the equations below with the Wintergreen gene length (1198nt) and protein length (358aa) respectively.

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Then, we use the parameters for degradation rates for proteins and mRNAs from Beijing PKU 2009 iGEM team:

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Since BSMT1 (Wintergreen) half-life has not been determined by MIT Team 2006, by the searching of this data, according to Zubieta et al.(2003), the average half-life for salicylic acid methyltransferases are about 40 min.

For determining the degradation rate of average mRNA we used the information from Selinger’s team (2003). They carried several experiments for finding average mRNA half-life in E. coli. They used mRNAs about 1100 nt concluding they have an average half-life of 5min. So with this we found the average mRNA half-life of BSMT1 was 5.44min.

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With all these information we could find the degradation rates for both transcription and translation of BSMT1.

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