Team:CIDEB-UANL Mexico/labwork methods

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iGEM CIDEB 2014 - Project

Methods

Capture Module

UV Experimentation

This experiment was designed in order to know if the UV promoter is working properly.

Procedure:

1. Inoculate by streak 2 Petri dishes with NhaS DNA in pSB1C3 Red bacteria

2. Repeat step 1 but with NhaS DNA in pSB1C3 White bacteria

3. Let them grow during one day in the incubator at 37°C

4. Expose the four Petri dishes to UV irradiation (302nm) during 2 hours

5. Take photos each 10 minutes and wait for results. (You can also take video during the 2 hours instead of the photos)

Go to results

Viability in salt (experiment 1)

Bacteria transformed with the capture plasmid were inoculated in Petri dishes with different concentrations of salt

IMG_0317

Image 1. All the 18 Petri dishes inoculated with NhaS Red in pSB1C3 of all the 9 used concentrations.

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