Team:CIDEB-UANL Mexico/labwork methods

From 2014hs.igem.org

(Difference between revisions)
Line 352: Line 352:
<p>Bacteria transformed with the capture plasmid were inoculated in Petri dishes with different concentrations of salt</p>
<p>Bacteria transformed with the capture plasmid were inoculated in Petri dishes with different concentrations of salt</p>
-
<center><p><img width=379 height=258 src="https://2014hs.igem.org/File:Rojas_NhaS_experiment_1.jpg"
+
<center><p><img width=380 height=260 src="https://2014hs.igem.org/File:Rojas_NhaS_experiment_1.jpg"
align=center hspace=12 alt="IMG_0317"></p>
align=center hspace=12 alt="IMG_0317"></p>
-
 
<p><center><b>Image 1. </b>All the 18 Petri dishes inoculated with NhaS Red in pSB1C3 of all the 9 used concentrations.</p></center>
<p><center><b>Image 1. </b>All the 18 Petri dishes inoculated with NhaS Red in pSB1C3 of all the 9 used concentrations.</p></center>
 +
<br>
 +
 +
<center><p><img width=380 height=260 src="https://2014hs.igem.org/File:Blancas_NhaS_experimen_1.jpg"
 +
align=center hspace=12 alt="IMG_0317"></p>
 +
<p><center><b>Image 2. </b>All the 18 Petri dishes inoculated with NhaS White in pSB1C3 of all the 9 used concentrations.</p></center>
 +
<br>
 +
 +
<center><p><img width=380 height=260 src="https://2014hs.igem.org/File:Control_Expriment_1_NhaS_.jpg"
 +
align=center hspace=12 alt="IMG_0317"></p>
 +
<p><center><b>Image 3. </b>All the 18 Petri dishes inoculated with the Control bacteria of all the 9 used concentrations.</p></center>
 +
<p>All the bacteria containing the NhaS in pSB1C3 (Red and White) survived to a 10% concentration of salt.</p>
 +
<p>None of the control group lived in any concentration of salt.</p>
 +
<br>
 +
 +
<center><p><img width=262 height=403 src="https://2014hs.igem.org/File:Experiment_1_Maximum_concentration_of_salt..jpg"
 +
align=center hspace=12 alt="IMG_0317"></p>
 +
<p><center><b>Image 4. </b>Nine Petri dishes with the maximum concentration of salt (10%) used in this experiment. From up to bottom: NhaS Red in pSB1C3, NhaS White in pSB1C3 and the Control bacteria. </p></center>
 +
<br>
 +
<div style="text-align: right;"><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/labwork_methods#"><font color="blue">Return to the Top</font></a></p></div>
<div style="text-align: right;"><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/labwork_methods#"><font color="blue">Return to the Top</font></a></p></div>

Revision as of 01:41, 15 June 2014

iGEM CIDEB 2014 - Project

Methods

Capture Module

UV Experimentation

This experiment was designed in order to know if the UV promoter is working properly.

Procedure:

1. Inoculate by streak 2 Petri dishes with NhaS DNA in pSB1C3 Red bacteria

2. Repeat step 1 but with NhaS DNA in pSB1C3 White bacteria

3. Let them grow during one day in the incubator at 37°C

4. Expose the four Petri dishes to UV irradiation (302nm) during 2 hours

5. Take photos each 10 minutes and wait for results. (You can also take video during the 2 hours instead of the photos)

Go to results

Viability in salt (experiment 1)

Bacteria transformed with the capture plasmid were inoculated in Petri dishes with different concentrations of salt

IMG_0317

Image 1. All the 18 Petri dishes inoculated with NhaS Red in pSB1C3 of all the 9 used concentrations.


IMG_0317

Image 2. All the 18 Petri dishes inoculated with NhaS White in pSB1C3 of all the 9 used concentrations.


IMG_0317

Image 3. All the 18 Petri dishes inoculated with the Control bacteria of all the 9 used concentrations.

All the bacteria containing the NhaS in pSB1C3 (Red and White) survived to a 10% concentration of salt.

None of the control group lived in any concentration of salt.


IMG_0317

Image 4. Nine Petri dishes with the maximum concentration of salt (10%) used in this experiment. From up to bottom: NhaS Red in pSB1C3, NhaS White in pSB1C3 and the Control bacteria.


iGEM CIDEB 2014 - Footer