Team:CIDEB-UANL Mexico/labwork methods
From 2014hs.igem.org
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<p>Bacteria transformed with the capture plasmid were inoculated in Petri dishes with different concentrations of salt</p> | <p>Bacteria transformed with the capture plasmid were inoculated in Petri dishes with different concentrations of salt</p> | ||
- | <center><p><img width= | + | <center><p><img width=380 height=260 src="https://2014hs.igem.org/File:Rojas_NhaS_experiment_1.jpg" |
align=center hspace=12 alt="IMG_0317"></p> | align=center hspace=12 alt="IMG_0317"></p> | ||
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<p><center><b>Image 1. </b>All the 18 Petri dishes inoculated with NhaS Red in pSB1C3 of all the 9 used concentrations.</p></center> | <p><center><b>Image 1. </b>All the 18 Petri dishes inoculated with NhaS Red in pSB1C3 of all the 9 used concentrations.</p></center> | ||
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+ | <center><p><img width=380 height=260 src="https://2014hs.igem.org/File:Blancas_NhaS_experimen_1.jpg" | ||
+ | align=center hspace=12 alt="IMG_0317"></p> | ||
+ | <p><center><b>Image 2. </b>All the 18 Petri dishes inoculated with NhaS White in pSB1C3 of all the 9 used concentrations.</p></center> | ||
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+ | <center><p><img width=380 height=260 src="https://2014hs.igem.org/File:Control_Expriment_1_NhaS_.jpg" | ||
+ | align=center hspace=12 alt="IMG_0317"></p> | ||
+ | <p><center><b>Image 3. </b>All the 18 Petri dishes inoculated with the Control bacteria of all the 9 used concentrations.</p></center> | ||
+ | <p>All the bacteria containing the NhaS in pSB1C3 (Red and White) survived to a 10% concentration of salt.</p> | ||
+ | <p>None of the control group lived in any concentration of salt.</p> | ||
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+ | <center><p><img width=262 height=403 src="https://2014hs.igem.org/File:Experiment_1_Maximum_concentration_of_salt..jpg" | ||
+ | align=center hspace=12 alt="IMG_0317"></p> | ||
+ | <p><center><b>Image 4. </b>Nine Petri dishes with the maximum concentration of salt (10%) used in this experiment. From up to bottom: NhaS Red in pSB1C3, NhaS White in pSB1C3 and the Control bacteria. </p></center> | ||
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<div style="text-align: right;"><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/labwork_methods#"><font color="blue">Return to the Top</font></a></p></div> | <div style="text-align: right;"><a href="https://2014hs.igem.org/Team:CIDEB-UANL_Mexico/labwork_methods#"><font color="blue">Return to the Top</font></a></p></div> |
Revision as of 01:41, 15 June 2014
Methods
Capture Module
UV Experimentation
This experiment was designed in order to know if the UV promoter is working properly.
Procedure:
1. Inoculate by streak 2 Petri dishes with NhaS DNA in pSB1C3 Red bacteria
2. Repeat step 1 but with NhaS DNA in pSB1C3 White bacteria
3. Let them grow during one day in the incubator at 37°C
4. Expose the four Petri dishes to UV irradiation (302nm) during 2 hours
5. Take photos each 10 minutes and wait for results. (You can also take video during the 2 hours instead of the photos)
Go to results
Viability in salt (experiment 1)
Bacteria transformed with the capture plasmid were inoculated in Petri dishes with different concentrations of salt
All the bacteria containing the NhaS in pSB1C3 (Red and White) survived to a 10% concentration of salt.
None of the control group lived in any concentration of salt.