Team:CIDEB-UANL Mexico/labwork methods

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<p><b>Capture Module</b></p>
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<p><b>UV Experimentation</b></p>
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<p>This experiment was designed in order to know if the UV promoter is working properly.</p>
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<p><b>Procedure:</b></p>
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<p><b>1.</b>  Inoculate by streak 2 Petri dishes with NhaS DNA in pSB1C3 Red bacteria</p>
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<p><b>2.</b>  Repeat step 1 but with NhaS DNA in pSB1C3 White bacteria</p>
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<p><b>3.</b>  Let them grow during one day in the incubator at 37°C</p>
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<p><b>4.</b>  Expose the four Petri dishes to UV irradiation (302nm) during 2 hours</p>
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<p><b>5.</b>  Take photos each 10 minutes and wait for results. (You can also take video during the 2 hours instead of the photos)</p>
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<p>Go to results</p>
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<p><b>Viability in salt (experiment 1)</b></p>
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<p>Bacteria transformed with the capture plasmid were inoculated in Petri dishes with different concentrations of salt</p>
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<center><p><img width=379 height=258 src="https://2014hs.igem.org/File:Rojas_NhaS_experiment_1.jpg"
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align=center hspace=12 alt="IMG_0317"></p>
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<p><center><b>Image 1. </b>All the 18 Petri dishes inoculated with NhaS Red in pSB1C3 of all the 9 used concentrations.</p></center>
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Revision as of 01:15, 15 June 2014

Methods Notebook Results Interpretations Conclusions Future Plans
iGEM CIDEB 2014 - Project

Methods

Capture Module

UV Experimentation

This experiment was designed in order to know if the UV promoter is working properly.

Procedure:

1. Inoculate by streak 2 Petri dishes with NhaS DNA in pSB1C3 Red bacteria

2. Repeat step 1 but with NhaS DNA in pSB1C3 White bacteria

3. Let them grow during one day in the incubator at 37°C

4. Expose the four Petri dishes to UV irradiation (302nm) during 2 hours

5. Take photos each 10 minutes and wait for results. (You can also take video during the 2 hours instead of the photos)

Go to results

Viability in salt (experiment 1)

Bacteria transformed with the capture plasmid were inoculated in Petri dishes with different concentrations of salt

IMG_0317

Image 1. All the 18 Petri dishes inoculated with NhaS Red in pSB1C3 of all the 9 used concentrations.

iGEM CIDEB 2014 - Footer

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