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From 2014hs.igem.org

Contents 1 Project 2 Abstract 3 Background 4 Step 4.1 Step1 4.2 Step2 4.3 Step3 4.4 Step4 4.5 Step5 4.6 Results/Conclusions

Project

Abstract

During consumption of some electronic facilities, such as TV, computer and mobiles, different wave length and frequency of electronic magnetic radiation (EMR) are emitted. EMR has strong penetration, gradually invades human’s body, and embarks different kind of diseases. Melittin is the principal active component of apitoxin (bee venom), consisting of 26 amino acids with the sequence GIGAVLKVLTTGLPALISWIKRKRQQ. Some studies showed that mellitin was resistive to EMR. We are going to construct the bacteria that can produce mellitin.

Background

As the students in an international school, we always face many different kinds of assessment events and each of them need to finished by computer. After using Internet whole day, our eyes would feel uncomfortable with innumerable distinct kind radiations. How can we deal with it? The eye is a complex organ, composed of many different structures with varying sensitivity to the effects of radiation. An easy way to look at the eye is to divide it into zones that can be examined with respect to their susceptibility to radiation effects. It is also noteworthy that the effects of radiation on the eye not only depend on the sensitivity of the tissue itself but also on the mode of radiation delivery.

Step

Step1

We have to got Part A and Part B's DNA before we digestion. Part A will be used after transformation and miniprep. We got Part B by artificial synthesis.

Step2

Digestion

Step3

Electrophoresis

Step4

Ligation

Step5

Sequencing

Results/Conclusions

What did you achieve over the course of your semester?