Team:Beijing HDFLS High/Project


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Beijing HDFLS iGEM 2013




The UV-responsing tandem generators for a wintergreen odor and protective melittin


The protective melittin generator is composed of two transcriptional devices: RecA (SOS) promoter (BBa_J22106) and synthesized melittin gene with enterokinase digested sequence. The protective generator takes as input extensive UV radiation and produces as output fusion melittin. Melittin can improve immunity and scavenging free radicals to alleviate the harm from electromagnetic radiation, such as blue light and UV.

The protective melittin generator.png

We further construct a biological system that produces a wintergreen odor and protective melittin under extensive UV radiation. The biological system includes two generators: the UV-responsing wintergreen odor generator (BBa_K994000) and the protective melittin generator. It not only can warn people of intense UV irradiation by a wintergreen odor, but also produce protective melittin.

Tandem Generators.png


Blue light from computer induces eye retina producing free radical

The electromagnetic spectrum, in order of increasing frequency and decreasing wavelength, can be divided, for practical engineering purposes, into radio waves, microwaves, infrared radiation, visible light, ultraviolet radiation, X-rays and gamma rays. The eyes of various organisms sense a relatively small range of frequencies of EMR called the visible spectrum or light. Higher frequencies (shorter wavelengths) correspond to proportionately more energy carried by each photon. Blue light is the most energetic portion of the visible light spectrum, it's less energetic than UV radiation but it also has the ability to penetrate into tissue and cause cellular damage.

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Blue light is the hardest for the eye to focus on. We actually see a halo around bright blue light. Blue light scatters more inside the eyeball than other colors, producing more glare, more eyestrain and more fatigue. Intense blue light can cause damage to the retina, because blue is the hardest color for the retina to handle. The blue light induce that retinal produce free radicals. These radicals can lead to retinal pigment epithelial cell death. Then some light-sensitive cells lacks of nutrient, which results in visual impairment.

Blue light.png

Melittin has funcition of scavenging free radical

The old paint and song implies that the early ancient civilization was conscious of the possible virtues from bee stings.


Melittin is the main component (50~55%) of bee venom, which has multiple functions.


Melittin function.png


I: Parts of Protective Mleittin Generators.


Part A: Rec A (SOS) promoter

Extensive UV irradiation impings on heavily DNA damage and results in a single-strand DNA occuring, which activates RecA protein.Then,RecA protein leads to the expression of about 15 different repair proteins to rescue DNA damage.RecA protein is the central regulator in SOS repair system.Rec A (SOS) Promoter is available in 2004 registry part plate.


Part B: Fusion melittin

We synthesized fusion melittin gene with enterokinase digestion site. Melittin has the function of scavenging free radicals.


II: Parts of Tandem Generators.

Part A: Winter Green Odor Generator

The generator was constructed last year, and can produce wintergreen odor under extensive UV radiation

Part B: Protective melittin Genertor

This is new constructed this year, and can produce protective melittin under extensive UV radiation


METHOD I: The Protective Melittin Generator

Method I.jpg

METHOD II: The Tandem Generators

We construct tandem Generators, using similar method with protective melittin generator


Result I:The Protective Melittin Generator

Result Plate 2.jpg
Electrophoresis Map after Restriction Digest
Result Map4.jpg
DNA Sequencing and Submitting Part


Result II:Tandem Generators

DNA Sequencing and Submitting Part



Step1 Transformation and Miniprep

We get Part A plasmid from registry, and construct Part B plasmid.


Step2 Digestion


Step3 Ligation


Step4 Tansformation, Miniprep and Digestion again


Step5 Sequencing