Team:Beijing HDFLS High/Project

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Beijing HDFLS iGEM 2013

 



Contents

TITLE

The UV-responsing tandem generators for a wintergreen odor and protective melittin

ABSTRACT

The protective melittin generator is composed of two transcriptional devices: RecA (SOS) promoter (BBa_J22106) and synthesized melittin gene with enterokinase digested sequence. The protective generator takes as input extensive UV radiation and produces as output fusion melittin. Melittin can improve immunity and scavenging free radicals to alleviate the harm from electromagnetic radiation, such as blue light and UV.

The protective melittin generator.png

We further construct a biological system that produces a wintergreen odor and protective melittin under extensive UV radiation. The biological system includes two generators: the UV-responsing wintergreen odor generator (BBa_K994000) and the protective melittin generator. It not only can warn people of intense UV irradiation by a wintergreen odor, but also produce protective melittin.

Mellitin generator.png

BACKGROUND

The electromagnetic spectrum, in order of increasing frequency and decreasing wavelength, can be divided, for practical engineering purposes, into radio waves, microwaves, infrared radiation, visible light, ultraviolet radiation, X-rays and gamma rays. The eyes of various organisms sense a relatively small range of frequencies of EMR called the visible spectrum or light. Higher frequencies (shorter wavelengths) correspond to proportionately more energy carried by each photon. Blue light is the most energetic portion of the visible light spectrum, it's less energetic than UV radiation but it also has the ability to penetrate into tissue and cause cellular damage.

2000px-EM spectrum.png

Blue light is the hardest for the eye to focus on. We actually see a halo around bright blue light. Blue light scatters more inside the eyeball than other colors, producing more glare, more eyestrain and more fatigue. Intense blue light can cause damage to the retina, because blue is the hardest color for the retina to handle.

The old paint and song implies that the early ancient civilization was conscious of the possible virtues from bee stings.

Bee3.png

Melittin is the main component (50~55%) of bee venom, which has multiple functions.

Melittin3.png


Melittin function.png

PART AND METHOD

RESULTS AND CONCLUSIONS

Step

Step1

We have to got Part A and Part B's DNA before we digestion. Part A will be used after transformation and miniprep. We got Part B by artificial synthesis.

Step2

Digestion

Step3

Electrophoresis

Step4

Ligation

Step5

Sequencing