http://2014hs.igem.org/wiki/index.php?title=Team:Beijing_HDFLS_High/Project&feed=atom&action=historyTeam:Beijing HDFLS High/Project - Revision history2024-03-28T10:56:43ZRevision history for this page on the wikiMediaWiki 1.16.5http://2014hs.igem.org/wiki/index.php?title=Team:Beijing_HDFLS_High/Project&diff=22501&oldid=prevHongmei: /* STEP */2014-06-20T12:45:16Z<p><span class="autocomment">STEP</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>=<del class="diffchange diffchange-inline">STEP</del>=</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>=<ins class="diffchange diffchange-inline">STEPS</ins>=</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Step1 Transformation and Miniprep===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Step1 Transformation and Miniprep===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We get Part A plasmid from registry, and construct Part B plasmid.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We get Part A plasmid from registry, and construct Part B plasmid.</div></td></tr>
</table>Hongmeihttp://2014hs.igem.org/wiki/index.php?title=Team:Beijing_HDFLS_High/Project&diff=22498&oldid=prevHongmei: /* Part A: Rec A (SOS) promoter */2014-06-20T12:44:14Z<p><span class="autocomment">Part A: Rec A (SOS) promoter</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Part A: Rec A (SOS) promoter==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Part A: Rec A (SOS) promoter==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Extensive UV irradiation impings on heavily DNA damage and results in a single-strand DNA occuring, which activates RecA protein.Then,RecA protein leads to the expression of about 15 different repair proteins to rescue DNA damage.RecA protein is the central regulator in SOS repair system.Rec A (SOS) Promoter is available in <del class="diffchange diffchange-inline">2004 </del>registry part plate.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Extensive UV irradiation impings on heavily DNA damage and results in a single-strand DNA occuring, which activates RecA protein.Then,RecA protein leads to the expression of about 15 different repair proteins to rescue DNA damage.RecA protein is the central regulator in SOS repair system.Rec A (SOS) Promoter is available in registry part plate.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image: Part A.jpg|center|400px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image: Part A.jpg|center|400px]]</div></td></tr>
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</table>Hongmeihttp://2014hs.igem.org/wiki/index.php?title=Team:Beijing_HDFLS_High/Project&diff=22464&oldid=prevHongmei: /* Part B: Protective Melittin Genertor */2014-06-20T12:10:36Z<p><span class="autocomment">Part B: Protective Melittin Genertor</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Part B: Protective Melittin Genertor==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Part B: Protective Melittin Genertor==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">This </del>is <del class="diffchange diffchange-inline">new </del>constructed this year, and can produce protective melittin under extensive UV radiation</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The generator </ins>is constructed this year, and can produce protective melittin under extensive UV radiation</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=METHODS=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=METHODS=</div></td></tr>
</table>Hongmeihttp://2014hs.igem.org/wiki/index.php?title=Team:Beijing_HDFLS_High/Project&diff=22463&oldid=prevHongmei: /* Part A: Rec A (SOS) promoter */2014-06-20T12:09:16Z<p><span class="autocomment">Part A: Rec A (SOS) promoter</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Part A: Rec A (SOS) promoter==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Part A: Rec A (SOS) promoter==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Extensive UV irradiation impings on heavily DNA damage and results in a single-strand DNA occuring, which activates RecA protein.Then,RecA protein leads to the expression of about 15 different repair proteins to rescue DNA damage.RecA protein is the central regulator in SOS repair system.Rec A (SOS) Promoter is available in 2004 registry part plate.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Extensive UV irradiation impings on heavily DNA damage and results in a single-strand DNA occuring, which activates RecA protein.Then,RecA protein leads to the expression of about 15 different repair proteins to rescue DNA damage.RecA protein is the central regulator in SOS repair system.Rec A (SOS) Promoter is available in 2004 registry part plate.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image: Part A.jpg|center|<del class="diffchange diffchange-inline">700px</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image: Part A.jpg|center|<ins class="diffchange diffchange-inline">400px</ins>]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Part B: Fusion melittin ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Part B: Fusion melittin ==</div></td></tr>
</table>Hongmeihttp://2014hs.igem.org/wiki/index.php?title=Team:Beijing_HDFLS_High/Project&diff=22462&oldid=prevHongmei: /* I: Parts of Protective Mleittin Generators. */2014-06-20T12:08:34Z<p><span class="autocomment">I: Parts of Protective Mleittin Generators.</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=PARTS=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=PARTS=</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>=I: Parts of Protective <del class="diffchange diffchange-inline">Mleittin </del>Generators.=</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>=I: Parts of Protective <ins class="diffchange diffchange-inline">Melittin </ins>Generators.=</div></td></tr>
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</table>Hongmeihttp://2014hs.igem.org/wiki/index.php?title=Team:Beijing_HDFLS_High/Project&diff=22461&oldid=prevHongmei: /* TITLE */2014-06-20T12:07:22Z<p><span class="autocomment">TITLE</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The protective melittin generator </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The protective melittin generator<ins class="diffchange diffchange-inline">; </ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The UV-responsing tandem generators for a wintergreen odor and protective melittin</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The UV-responsing tandem generators for a wintergreen odor and protective melittin<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=ABSTRACT=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=ABSTRACT=</div></td></tr>
</table>Hongmeihttp://2014hs.igem.org/wiki/index.php?title=Team:Beijing_HDFLS_High/Project&diff=22460&oldid=prevHongmei: /* TITLE */2014-06-20T12:06:58Z<p><span class="autocomment">TITLE</span></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The protective melittin generator </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The UV-responsing tandem generators for a wintergreen odor and protective melittin</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The UV-responsing tandem generators for a wintergreen odor and protective melittin</div></td></tr>
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</table>Hongmeihttp://2014hs.igem.org/wiki/index.php?title=Team:Beijing_HDFLS_High/Project&diff=22252&oldid=prevHongmei: /* ABSTRACT */2014-06-20T08:34:30Z<p><span class="autocomment">ABSTRACT</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=ABSTRACT=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=ABSTRACT=</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The protective melittin generator is composed of two transcriptional devices: RecA (SOS) promoter (BBa_J22106) and synthesized melittin gene with enterokinase digested sequence. The protective generator takes as input extensive UV radiation and produces as output fusion melittin. Melittin can improve immunity and scavenging free radicals to alleviate the harm from electromagnetic radiation, such as blue light and UV.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The protective melittin generator is composed of two transcriptional devices: RecA (SOS) promoter (BBa_J22106) and synthesized melittin gene with enterokinase digested sequence. The protective generator takes as input extensive UV radiation and produces as output fusion melittin. Melittin can improve immunity and scavenging free radicals to alleviate the harm from electromagnetic radiation, such as blue light and UV.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:The_protective_melittin_generator.png|center|<del class="diffchange diffchange-inline">600px</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:The_protective_melittin_generator.png|center|<ins class="diffchange diffchange-inline">400px</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We further construct a biological system that produces a wintergreen odor and protective melittin under extensive UV radiation. The biological system includes two generators: the UV-responsing wintergreen odor generator (BBa_K994000) and the protective melittin generator. It not only can warn people of intense UV irradiation by a wintergreen odor, but also produce protective melittin.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We further construct a biological system that produces a wintergreen odor and protective melittin under extensive UV radiation. The biological system includes two generators: the UV-responsing wintergreen odor generator (BBa_K994000) and the protective melittin generator. It not only can warn people of intense UV irradiation by a wintergreen odor, but also produce protective melittin.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
</table>Hongmeihttp://2014hs.igem.org/wiki/index.php?title=Team:Beijing_HDFLS_High/Project&diff=22247&oldid=prevHongmei: /* I: Parts of Protective Mleittin Generators. */2014-06-20T08:27:11Z<p><span class="autocomment">I: Parts of Protective Mleittin Generators.</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=I: Parts of Protective Mleittin Generators.=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=I: Parts of Protective Mleittin Generators.=</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Part A: Rec A (SOS) promoter==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Part A: Rec A (SOS) promoter==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We synthesized fusion melittin gene with enterokinase digestion site. Melittin has the function of scavenging free radicals.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We synthesized fusion melittin gene with enterokinase digestion site. Melittin has the function of scavenging free radicals.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:melittin.jpg|center|700px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:melittin.jpg|center|700px]]</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=II: Parts of Tandem Generators.=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=II: Parts of Tandem Generators.=</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Part A: Wintergreen Odor Generator==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Part A: Wintergreen Odor Generator==</div></td></tr>
</table>Hongmeihttp://2014hs.igem.org/wiki/index.php?title=Team:Beijing_HDFLS_High/Project&diff=22233&oldid=prevHongmei: /* Part B: Protective melittin Genertor */2014-06-20T08:22:18Z<p><span class="autocomment">Part B: Protective melittin Genertor</span></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 08:22, 20 June 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The generator was constructed last year, and can produce wintergreen odor under extensive UV radiation</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The generator was constructed last year, and can produce wintergreen odor under extensive UV radiation</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==Part B: Protective <del class="diffchange diffchange-inline">melittin </del>Genertor==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==Part B: Protective <ins class="diffchange diffchange-inline">Melittin </ins>Genertor==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This is new constructed this year, and can produce protective melittin under extensive UV radiation</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This is new constructed this year, and can produce protective melittin under extensive UV radiation</div></td></tr>
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</table>Hongmei