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Team:Acton-BoxboroughRHS/Research
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E.coli has a higher pH range and may be easier to assemble, but may be less productive. Yeast will probably be more productive in the long run but is otherwise inferior
we need an open reading frame (orf) a ribosome binding site (rbs), promoter, and terminator at least 3-5 enzymes: pepsin, salivary amylase, trypsin, chymotripsin, pancreatic amylase
This image shows the template for a typical plasmid requirement
The Terminator is the 'last' region of the plasmid, where RNA polymerase stops transcription. There are two main types of terminators in prokaryotes, rho-dependent terminators, and rho-independent terminators. Rho-dependent termination bases its function on the Rho protein, which causes the RNA polymerase to fall disassociate form the plasmid. Rho-independent termination does not use Rho to disassociate the RNA polymerase, but instead stops by the DNA sequence, here's how it works. The DNA sequence contains a two regions that are rich in cytosine and guanine. When this is transcribed, those regions on the RNA form hydrogen bonds to itself: effectively forming a 'loop' which disassociates the DNA polymerase from the DNA.
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