http://2014hs.igem.org/wiki/index.php?title=Team:Acton-BoxboroughRHS/Notebook&feed=atom&action=history
Team:Acton-BoxboroughRHS/Notebook - Revision history
2024-03-28T11:14:49Z
Revision history for this page on the wiki
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http://2014hs.igem.org/wiki/index.php?title=Team:Acton-BoxboroughRHS/Notebook&diff=28189&oldid=prev
17malim at 02:57, 14 September 2014
2014-09-14T02:57:20Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <img height=220px width=950px src="https://c9.io/ab_igem/igem2014<del class="diffchange diffchange-inline">/workspace</del>/copy.jpg" alt="ABRHS"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <img height=220px width=950px src="https://<ins class="diffchange diffchange-inline">preview.</ins>c9.io/ab_igem/igem2014/copy.jpg<ins class="diffchange diffchange-inline">?_c9_id=livepreview0&_c9_host=https://ide.c9.io</ins>" alt="ABRHS"></div></td></tr>
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17malim
http://2014hs.igem.org/wiki/index.php?title=Team:Acton-BoxboroughRHS/Notebook&diff=25806&oldid=prev
15kuffnerc at 00:45, 21 June 2014
2014-06-21T00:45:35Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> The iGEM Jamboree Fee</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> The iGEM Jamboree Fee</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> Our Project-lab Notebook</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> Our Project-lab Notebook</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li> More on the parts we will use</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li> More on the parts we will use<ins class="diffchange diffchange-inline">- very few of the original parts are in the catalog</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> More frequent meeting dates!</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> More frequent meeting dates!</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ol></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ol></div></td></tr>
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15kuffnerc
http://2014hs.igem.org/wiki/index.php?title=Team:Acton-BoxboroughRHS/Notebook&diff=25777&oldid=prev
15kuffnerc at 00:40, 21 June 2014
2014-06-21T00:40:31Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> Today, the <del class="diffchange diffchange-inline">amylase </del>lab was started and finished. We also plan to have a Google Hangout on thursday, since many people are busy. The following topics were discussed and worked on.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> Today, the <ins class="diffchange diffchange-inline">High Efficiency lab (see lab tab) </ins>lab was started and finished <ins class="diffchange diffchange-inline">using part BBa_K523006</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">for alpha amylase. This lab failed, leading to the conclusion that our competent cells had not been properly maintained and were no longer effective</ins>. We also plan to have a Google Hangout on thursday, since many people are busy. The following topics were discussed and worked on.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> The iGEM wiki (Research Tab update, Home page, Team page, etc.)</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li> The iGEM wiki (Research Tab update, Home page, Team page, etc.)</li></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Testing the bacteria with beans (or cherries), needs to take place as well.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Testing the bacteria with beans (or cherries), needs to take place as well.</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><h3></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> June 16 2014</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> </h3></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Due to the failure of the High Efficiency lab, we performed a new procedure: the Main Project Lab (see lab tab). This procedure does not rely on the competent cells, but instead uses CaCl2 to catalyze transformation.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">We did not see any growth the next day, but we had some leftover cells that we had not put on the plates. These leftovers were plated in the hope that they had transformed, but they had not.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p></ins></div></td></tr>
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15kuffnerc
http://2014hs.igem.org/wiki/index.php?title=Team:Acton-BoxboroughRHS/Notebook&diff=25674&oldid=prev
15kuffnerc at 00:20, 21 June 2014
2014-06-21T00:20:32Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> After this meeting, we carried out the 3A assembly protocol twice (see protocols 1-5 in lab tab). <del class="diffchange diffchange-inline">we failed to </del>achieve growth on the chloramphenicol plates <del class="diffchange diffchange-inline">both times</del>, possibly due to our failure to properly freeze the competent cells.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> After this meeting, we carried out the 3A assembly protocol twice (see protocols 1-5 in lab tab). <ins class="diffchange diffchange-inline">We did not </ins>achieve growth on the chloramphenicol plates <ins class="diffchange diffchange-inline">either time</ins>, possibly due to our failure to properly freeze the competent cells.</div></td></tr>
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15kuffnerc
http://2014hs.igem.org/wiki/index.php?title=Team:Acton-BoxboroughRHS/Notebook&diff=25666&oldid=prev
15kuffnerc at 00:19, 21 June 2014
2014-06-21T00:19:45Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> After this meeting, we carried out the 3A assembly protocol twice (see protocols 1-5 in lab tab). we failed to achieve growth both times, possibly due to our failure to properly freeze the competent cells.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> After this meeting, we carried out the 3A assembly protocol twice (see protocols 1-5 in lab tab). we failed to achieve growth <ins class="diffchange diffchange-inline">on the chloramphenicol plates </ins>both times, possibly due to our failure to properly freeze the competent cells.</div></td></tr>
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15kuffnerc
http://2014hs.igem.org/wiki/index.php?title=Team:Acton-BoxboroughRHS/Notebook&diff=25655&oldid=prev
15kuffnerc at 00:18, 21 June 2014
2014-06-21T00:18:10Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <del class="diffchange diffchange-inline">On a side note, after </del>this meeting, <del class="diffchange diffchange-inline">the growth for </del>the 3A assembly <del class="diffchange diffchange-inline">kit has had some trouble </del>(<del class="diffchange diffchange-inline">now fixed</del>) <del class="diffchange diffchange-inline">twice</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <ins class="diffchange diffchange-inline">After </ins>this meeting, <ins class="diffchange diffchange-inline">we carried out </ins>the 3A assembly <ins class="diffchange diffchange-inline">protocol twice </ins>(<ins class="diffchange diffchange-inline">see protocols 1-5 in lab tab</ins>)<ins class="diffchange diffchange-inline">. we failed to achieve growth both times, possibly due to our failure to properly freeze the competent cells</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </p></div></td></tr>
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15kuffnerc
http://2014hs.igem.org/wiki/index.php?title=Team:Acton-BoxboroughRHS/Notebook&diff=17114&oldid=prev
17malim at 18:10, 17 June 2014
2014-06-17T18:10:41Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014hs.igem.org/Team:Acton-BoxboroughRHS/Research">Research</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014hs.igem.org/Team:Acton-BoxboroughRHS/Research">Research</a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014hs.igem.org/Team:Acton-BoxboroughRHS/Labs">Labs</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014hs.igem.org/Team:Acton-BoxboroughRHS/Labs">Labs</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <li><a href="https://2014hs.igem.org/Team:Acton-BoxboroughRHS/Template">Template</a></li></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></div></td></tr>
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17malim
http://2014hs.igem.org/wiki/index.php?title=Team:Acton-BoxboroughRHS/Notebook&diff=12210&oldid=prev
17malim at 02:29, 13 June 2014
2014-06-13T02:29:22Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <div <del class="diffchange diffchange-inline">background="https://c9.io/emykion/new_work_space/workspace/paper.jpg" </del>id="contents" style="border: 1px solid black; font-family:Arial; font-size:12px; width:900px;background-color:white;position:relative;top:50px"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <div <ins class="diffchange diffchange-inline"> </ins>id="contents" style="border: 1px solid black; font-family:Arial; font-size:12px; width:900px;background-color:white;position:relative;top:50px"></div></td></tr>
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17malim
http://2014hs.igem.org/wiki/index.php?title=Team:Acton-BoxboroughRHS/Notebook&diff=12209&oldid=prev
17malim at 02:28, 13 June 2014
2014-06-13T02:28:43Z
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17malim
http://2014hs.igem.org/wiki/index.php?title=Team:Acton-BoxboroughRHS/Notebook&diff=12207&oldid=prev
17malim at 02:27, 13 June 2014
2014-06-13T02:27:58Z
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:27, 13 June 2014</td>
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17malim