Team:AUC TURKEY/Results/Cloning

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  <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1"/>
 +
  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2"/>
 +
  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3"/>
 +
  <w:LsdException Locked="false" Priority="70" Name="Dark List"/>
 +
  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading"/>
 +
  <w:LsdException Locked="false" Priority="72" Name="Colorful List"/>
 +
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid"/>
 +
  <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="61" Name="Light List Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 1"/>
 +
  <w:LsdException Locked="false" SemiHidden="true" Name="Revision"/>
 +
  <w:LsdException Locked="false" Priority="34" QFormat="true"
 +
  Name="List Paragraph"/>
 +
  <w:LsdException Locked="false" Priority="29" QFormat="true" Name="Quote"/>
 +
  <w:LsdException Locked="false" Priority="30" QFormat="true"
 +
  Name="Intense Quote"/>
 +
  <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="61" Name="Light List Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="61" Name="Light List Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="61" Name="Light List Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="61" Name="Light List Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="61" Name="Light List Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="19" QFormat="true"
 +
  Name="Subtle Emphasis"/>
 +
  <w:LsdException Locked="false" Priority="21" QFormat="true"
 +
  Name="Intense Emphasis"/>
 +
  <w:LsdException Locked="false" Priority="31" QFormat="true"
 +
  Name="Subtle Reference"/>
 +
  <w:LsdException Locked="false" Priority="32" QFormat="true"
 +
  Name="Intense Reference"/>
 +
  <w:LsdException Locked="false" Priority="33" QFormat="true" Name="Book Title"/>
 +
  <w:LsdException Locked="false" Priority="37" SemiHidden="true"
 +
  UnhideWhenUsed="true" Name="Bibliography"/>
 +
  <w:LsdException Locked="false" Priority="39" SemiHidden="true"
 +
  UnhideWhenUsed="true" QFormat="true" Name="TOC Heading"/>
 +
  <w:LsdException Locked="false" Priority="41" Name="Plain Table 1"/>
 +
  <w:LsdException Locked="false" Priority="42" Name="Plain Table 2"/>
 +
  <w:LsdException Locked="false" Priority="43" Name="Plain Table 3"/>
 +
  <w:LsdException Locked="false" Priority="44" Name="Plain Table 4"/>
 +
  <w:LsdException Locked="false" Priority="45" Name="Plain Table 5"/>
 +
  <w:LsdException Locked="false" Priority="40" Name="Grid Table Light"/>
 +
  <w:LsdException Locked="false" Priority="46" Name="Grid Table 1 Light"/>
 +
  <w:LsdException Locked="false" Priority="47" Name="Grid Table 2"/>
 +
  <w:LsdException Locked="false" Priority="48" Name="Grid Table 3"/>
 +
  <w:LsdException Locked="false" Priority="49" Name="Grid Table 4"/>
 +
  <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark"/>
 +
  <w:LsdException Locked="false" Priority="51" Name="Grid Table 6 Colorful"/>
 +
  <w:LsdException Locked="false" Priority="52" Name="Grid Table 7 Colorful"/>
 +
  <w:LsdException Locked="false" Priority="46"
 +
  Name="Grid Table 1 Light Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="51"
 +
  Name="Grid Table 6 Colorful Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="Grid Table 7 Colorful Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="46"
 +
  Name="Grid Table 1 Light Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="51"
 +
  Name="Grid Table 6 Colorful Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="Grid Table 7 Colorful Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="46"
 +
  Name="Grid Table 1 Light Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="51"
 +
  Name="Grid Table 6 Colorful Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="Grid Table 7 Colorful Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="46"
 +
  Name="Grid Table 1 Light Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 4"/>
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  <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="51"
 +
  Name="Grid Table 6 Colorful Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="Grid Table 7 Colorful Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="46"
 +
  Name="Grid Table 1 Light Accent 5"/>
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  <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 5"/>
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  <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 5"/>
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  <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 5"/>
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  <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="51"
 +
  Name="Grid Table 6 Colorful Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="Grid Table 7 Colorful Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="46"
 +
  Name="Grid Table 1 Light Accent 6"/>
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  <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 6"/>
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  <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 6"/>
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  <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="51"
 +
  Name="Grid Table 6 Colorful Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="Grid Table 7 Colorful Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="46" Name="List Table 1 Light"/>
 +
  <w:LsdException Locked="false" Priority="47" Name="List Table 2"/>
 +
  <w:LsdException Locked="false" Priority="48" Name="List Table 3"/>
 +
  <w:LsdException Locked="false" Priority="49" Name="List Table 4"/>
 +
  <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark"/>
 +
  <w:LsdException Locked="false" Priority="51" Name="List Table 6 Colorful"/>
 +
  <w:LsdException Locked="false" Priority="52" Name="List Table 7 Colorful"/>
 +
  <w:LsdException Locked="false" Priority="46"
 +
  Name="List Table 1 Light Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 1"/>
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  <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 1"/>
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  <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="51"
 +
  Name="List Table 6 Colorful Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="List Table 7 Colorful Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="46"
 +
  Name="List Table 1 Light Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 2"/>
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  <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="51"
 +
  Name="List Table 6 Colorful Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="List Table 7 Colorful Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="46"
 +
  Name="List Table 1 Light Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 3"/>
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  <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="51"
 +
  Name="List Table 6 Colorful Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="List Table 7 Colorful Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="46"
 +
  Name="List Table 1 Light Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 4"/>
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  <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 4"/>
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  <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="51"
 +
  Name="List Table 6 Colorful Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="List Table 7 Colorful Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="46"
 +
  Name="List Table 1 Light Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="51"
 +
  Name="List Table 6 Colorful Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="List Table 7 Colorful Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="46"
 +
  Name="List Table 1 Light Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="51"
 +
  Name="List Table 6 Colorful Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="List Table 7 Colorful Accent 6"/>
 +
</w:LatentStyles>
 +
</xml><![endif]-->
 +
<style>
 +
<!--
 +
/* Font Definitions */
 +
@font-face
 +
{font-family:"Cambria Math";
 +
panose-1:2 4 5 3 5 4 6 3 2 4;
 +
mso-font-charset:1;
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mso-generic-font-family:roman;
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mso-font-format:other;
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mso-font-pitch:variable;
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mso-font-signature:0 0 0 0 0 0;}
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@font-face
 +
{font-family:Calibri;
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panose-1:2 15 5 2 2 2 4 3 2 4;
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mso-font-charset:162;
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mso-generic-font-family:swiss;
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mso-font-pitch:variable;
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mso-font-signature:-536870145 1073786111 1 0 415 0;}
 +
@font-face
 +
{font-family:"Trebuchet MS";
 +
panose-1:2 11 6 3 2 2 2 2 2 4;
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mso-font-charset:162;
 +
mso-generic-font-family:swiss;
 +
mso-font-pitch:variable;
 +
mso-font-signature:647 3 0 0 159 0;}
 +
/* Style Definitions */
 +
p.MsoNormal, li.MsoNormal, div.MsoNormal
 +
{mso-style-unhide:no;
 +
mso-style-qformat:yes;
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mso-style-parent:"";
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margin:0cm;
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margin-bottom:.0001pt;
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line-height:115%;
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mso-pagination:widow-orphan;
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font-size:11.0pt;
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font-family:"Arial","sans-serif";
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:minor-fareast;
 +
color:black;}
 +
h1
 +
{mso-style-priority:9;
 +
mso-style-unhide:no;
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mso-style-qformat:yes;
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mso-style-link:"Başlık 1 Char";
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margin-top:10.0pt;
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margin-right:0cm;
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margin-bottom:0cm;
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margin-left:0cm;
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margin-bottom:.0001pt;
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line-height:115%;
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mso-pagination:widow-orphan;
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page-break-after:avoid;
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font-family:"Trebuchet MS","sans-serif";
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mso-fareast-font-family:"Times New Roman";
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mso-fareast-theme-font:minor-fareast;
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mso-bidi-font-family:"Times New Roman";
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color:black;
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font-weight:normal;}
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h2
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{mso-style-priority:9;
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mso-style-unhide:no;
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mso-style-qformat:yes;
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mso-style-link:"Başlık 2 Char";
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margin-top:10.0pt;
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margin-right:0cm;
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margin-bottom:0cm;
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margin-left:0cm;
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margin-bottom:.0001pt;
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line-height:115%;
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mso-pagination:widow-orphan;
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page-break-after:avoid;
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mso-outline-level:2;
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font-family:"Trebuchet MS","sans-serif";
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mso-fareast-font-family:"Times New Roman";
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mso-fareast-theme-font:minor-fareast;
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mso-bidi-font-family:"Times New Roman";
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color:black;}
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h3
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{mso-style-priority:9;
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mso-style-unhide:no;
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mso-style-qformat:yes;
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mso-style-link:"Başlık 3 Char";
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margin-top:8.0pt;
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margin-right:0cm;
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margin-bottom:0cm;
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margin-bottom:.0001pt;
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font-size:12.0pt;
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font-family:"Trebuchet MS","sans-serif";
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mso-fareast-font-family:"Times New Roman";
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mso-fareast-theme-font:minor-fareast;
 +
mso-bidi-font-family:"Times New Roman";
 +
color:#666666;}
 +
h4
 +
{mso-style-priority:9;
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mso-style-unhide:no;
 +
mso-style-qformat:yes;
 +
mso-style-link:"Başlık 4 Char";
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margin-top:8.0pt;
 +
margin-right:0cm;
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margin-bottom:0cm;
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margin-left:0cm;
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margin-bottom:.0001pt;
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mso-pagination:widow-orphan;
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page-break-after:avoid;
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font-family:"Trebuchet MS","sans-serif";
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mso-fareast-font-family:"Times New Roman";
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mso-fareast-theme-font:minor-fareast;
 +
mso-bidi-font-family:"Times New Roman";
 +
color:#666666;
 +
font-weight:normal;
 +
text-decoration:underline;
 +
text-underline:single;}
 +
h5
 +
{mso-style-priority:9;
 +
mso-style-unhide:no;
 +
mso-style-qformat:yes;
 +
mso-style-link:"Başlık 5 Char";
 +
margin-top:8.0pt;
 +
margin-right:0cm;
 +
margin-bottom:0cm;
 +
margin-left:0cm;
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margin-bottom:.0001pt;
 +
line-height:115%;
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mso-pagination:widow-orphan;
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page-break-after:avoid;
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font-size:11.0pt;
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font-family:"Trebuchet MS","sans-serif";
 +
mso-fareast-font-family:"Times New Roman";
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mso-fareast-theme-font:minor-fareast;
 +
mso-bidi-font-family:"Times New Roman";
 +
color:#666666;
 +
font-weight:normal;}
 +
h6
 +
{mso-style-priority:9;
 +
mso-style-unhide:no;
 +
mso-style-qformat:yes;
 +
mso-style-link:"Başlık 6 Char";
 +
margin-top:8.0pt;
 +
margin-right:0cm;
 +
margin-bottom:0cm;
 +
margin-left:0cm;
 +
margin-bottom:.0001pt;
 +
line-height:115%;
 +
mso-pagination:widow-orphan;
 +
page-break-after:avoid;
 +
mso-outline-level:6;
 +
font-size:11.0pt;
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font-family:"Trebuchet MS","sans-serif";
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:minor-fareast;
 +
mso-bidi-font-family:"Times New Roman";
 +
color:#666666;
 +
font-weight:normal;
 +
font-style:italic;}
 +
p.MsoTitle, li.MsoTitle, div.MsoTitle
 +
{mso-style-priority:10;
 +
mso-style-unhide:no;
 +
mso-style-qformat:yes;
 +
mso-style-link:"Konu Başlığı Char";
 +
margin:0cm;
 +
margin-bottom:.0001pt;
 +
line-height:115%;
 +
mso-pagination:widow-orphan;
 +
page-break-after:avoid;
 +
font-size:21.0pt;
 +
font-family:"Trebuchet MS","sans-serif";
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:minor-fareast;
 +
mso-bidi-font-family:"Times New Roman";
 +
color:black;}
 +
p.MsoSubtitle, li.MsoSubtitle, div.MsoSubtitle
 +
{mso-style-priority:11;
 +
mso-style-unhide:no;
 +
mso-style-qformat:yes;
 +
mso-style-link:"Altyazı Char";
 +
margin-top:0cm;
 +
margin-right:0cm;
 +
margin-bottom:10.0pt;
 +
margin-left:0cm;
 +
line-height:115%;
 +
mso-pagination:widow-orphan;
 +
page-break-after:avoid;
 +
font-size:13.0pt;
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font-family:"Trebuchet MS","sans-serif";
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:minor-fareast;
 +
mso-bidi-font-family:"Times New Roman";
 +
color:#666666;
 +
font-style:italic;}
 +
span.Balk1Char
 +
{mso-style-name:"Başlık 1 Char";
 +
mso-style-priority:9;
 +
mso-style-unhide:no;
 +
mso-style-locked:yes;
 +
mso-style-link:"Başlık 1";
 +
mso-ansi-font-size:16.0pt;
 +
mso-bidi-font-size:16.0pt;
 +
font-family:"Calibri Light","sans-serif";
 +
mso-ascii-font-family:"Calibri Light";
 +
mso-ascii-theme-font:major-latin;
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:major-fareast;
 +
mso-hansi-font-family:"Calibri Light";
 +
mso-hansi-theme-font:major-latin;
 +
mso-bidi-font-family:"Times New Roman";
 +
mso-bidi-theme-font:major-bidi;
 +
color:#2E74B5;
 +
mso-themecolor:accent1;
 +
mso-themeshade:191;}
 +
span.Balk2Char
 +
{mso-style-name:"Başlık 2 Char";
 +
mso-style-noshow:yes;
 +
mso-style-priority:9;
 +
mso-style-unhide:no;
 +
mso-style-locked:yes;
 +
mso-style-link:"Başlık 2";
 +
mso-ansi-font-size:13.0pt;
 +
mso-bidi-font-size:13.0pt;
 +
font-family:"Calibri Light","sans-serif";
 +
mso-ascii-font-family:"Calibri Light";
 +
mso-ascii-theme-font:major-latin;
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:major-fareast;
 +
mso-hansi-font-family:"Calibri Light";
 +
mso-hansi-theme-font:major-latin;
 +
mso-bidi-font-family:"Times New Roman";
 +
mso-bidi-theme-font:major-bidi;
 +
color:#2E74B5;
 +
mso-themecolor:accent1;
 +
mso-themeshade:191;}
 +
span.Balk3Char
 +
{mso-style-name:"Başlık 3 Char";
 +
mso-style-noshow:yes;
 +
mso-style-priority:9;
 +
mso-style-unhide:no;
 +
mso-style-locked:yes;
 +
mso-style-link:"Başlık 3";
 +
mso-ansi-font-size:12.0pt;
 +
mso-bidi-font-size:12.0pt;
 +
font-family:"Calibri Light","sans-serif";
 +
mso-ascii-font-family:"Calibri Light";
 +
mso-ascii-theme-font:major-latin;
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:major-fareast;
 +
mso-hansi-font-family:"Calibri Light";
 +
mso-hansi-theme-font:major-latin;
 +
mso-bidi-font-family:"Times New Roman";
 +
mso-bidi-theme-font:major-bidi;
 +
color:#1F4D78;
 +
mso-themecolor:accent1;
 +
mso-themeshade:127;}
 +
span.Balk4Char
 +
{mso-style-name:"Başlık 4 Char";
 +
mso-style-noshow:yes;
 +
mso-style-priority:9;
 +
mso-style-unhide:no;
 +
mso-style-locked:yes;
 +
mso-style-link:"Başlık 4";
 +
mso-ansi-font-size:11.0pt;
 +
mso-bidi-font-size:11.0pt;
 +
font-family:"Calibri Light","sans-serif";
 +
mso-ascii-font-family:"Calibri Light";
 +
mso-ascii-theme-font:major-latin;
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:major-fareast;
 +
mso-hansi-font-family:"Calibri Light";
 +
mso-hansi-theme-font:major-latin;
 +
mso-bidi-font-family:"Times New Roman";
 +
mso-bidi-theme-font:major-bidi;
 +
color:#2E74B5;
 +
mso-themecolor:accent1;
 +
mso-themeshade:191;
 +
font-style:italic;}
 +
span.Balk5Char
 +
{mso-style-name:"Başlık 5 Char";
 +
mso-style-noshow:yes;
 +
mso-style-priority:9;
 +
mso-style-unhide:no;
 +
mso-style-locked:yes;
 +
mso-style-link:"Başlık 5";
 +
mso-ansi-font-size:11.0pt;
 +
mso-bidi-font-size:11.0pt;
 +
font-family:"Calibri Light","sans-serif";
 +
mso-ascii-font-family:"Calibri Light";
 +
mso-ascii-theme-font:major-latin;
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:major-fareast;
 +
mso-hansi-font-family:"Calibri Light";
 +
mso-hansi-theme-font:major-latin;
 +
mso-bidi-font-family:"Times New Roman";
 +
mso-bidi-theme-font:major-bidi;
 +
color:#2E74B5;
 +
mso-themecolor:accent1;
 +
mso-themeshade:191;}
 +
span.Balk6Char
 +
{mso-style-name:"Başlık 6 Char";
 +
mso-style-noshow:yes;
 +
mso-style-priority:9;
 +
mso-style-unhide:no;
 +
mso-style-locked:yes;
 +
mso-style-link:"Başlık 6";
 +
mso-ansi-font-size:11.0pt;
 +
mso-bidi-font-size:11.0pt;
 +
font-family:"Calibri Light","sans-serif";
 +
mso-ascii-font-family:"Calibri Light";
 +
mso-ascii-theme-font:major-latin;
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:major-fareast;
 +
mso-hansi-font-family:"Calibri Light";
 +
mso-hansi-theme-font:major-latin;
 +
mso-bidi-font-family:"Times New Roman";
 +
mso-bidi-theme-font:major-bidi;
 +
color:#1F4D78;
 +
mso-themecolor:accent1;
 +
mso-themeshade:127;}
 +
span.KonuBalChar
 +
{mso-style-name:"Konu Başlığı Char";
 +
mso-style-priority:10;
 +
mso-style-unhide:no;
 +
mso-style-locked:yes;
 +
mso-style-link:"Konu Başlığı";
 +
mso-ansi-font-size:28.0pt;
 +
mso-bidi-font-size:28.0pt;
 +
font-family:"Calibri Light","sans-serif";
 +
mso-ascii-font-family:"Calibri Light";
 +
mso-ascii-theme-font:major-latin;
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:major-fareast;
 +
mso-hansi-font-family:"Calibri Light";
 +
mso-hansi-theme-font:major-latin;
 +
mso-bidi-font-family:"Times New Roman";
 +
mso-bidi-theme-font:major-bidi;
 +
color:windowtext;
 +
letter-spacing:-.5pt;
 +
mso-font-kerning:14.0pt;}
 +
p.msotitlecxspfirst, li.msotitlecxspfirst, div.msotitlecxspfirst
 +
{mso-style-name:msotitlecxspfirst;
 +
mso-style-unhide:no;
 +
margin:0cm;
 +
margin-bottom:.0001pt;
 +
line-height:115%;
 +
mso-pagination:widow-orphan;
 +
page-break-after:avoid;
 +
font-size:21.0pt;
 +
font-family:"Trebuchet MS","sans-serif";
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:minor-fareast;
 +
mso-bidi-font-family:"Times New Roman";
 +
color:black;}
 +
p.msotitlecxspmiddle, li.msotitlecxspmiddle, div.msotitlecxspmiddle
 +
{mso-style-name:msotitlecxspmiddle;
 +
mso-style-unhide:no;
 +
margin:0cm;
 +
margin-bottom:.0001pt;
 +
line-height:115%;
 +
mso-pagination:widow-orphan;
 +
page-break-after:avoid;
 +
font-size:21.0pt;
 +
font-family:"Trebuchet MS","sans-serif";
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:minor-fareast;
 +
mso-bidi-font-family:"Times New Roman";
 +
color:black;}
 +
p.msotitlecxsplast, li.msotitlecxsplast, div.msotitlecxsplast
 +
{mso-style-name:msotitlecxsplast;
 +
mso-style-unhide:no;
 +
margin:0cm;
 +
margin-bottom:.0001pt;
 +
line-height:115%;
 +
mso-pagination:widow-orphan;
 +
page-break-after:avoid;
 +
font-size:21.0pt;
 +
font-family:"Trebuchet MS","sans-serif";
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:minor-fareast;
 +
mso-bidi-font-family:"Times New Roman";
 +
color:black;}
 +
span.AltyazChar
 +
{mso-style-name:"Altyazı Char";
 +
mso-style-priority:11;
 +
mso-style-unhide:no;
 +
mso-style-locked:yes;
 +
mso-style-link:Altyazı;
 +
mso-ansi-font-size:11.0pt;
 +
mso-bidi-font-size:11.0pt;
 +
font-family:"Calibri","sans-serif";
 +
mso-ascii-font-family:Calibri;
 +
mso-ascii-theme-font:minor-latin;
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:minor-fareast;
 +
mso-hansi-font-family:Calibri;
 +
mso-hansi-theme-font:minor-latin;
 +
mso-bidi-font-family:"Times New Roman";
 +
mso-bidi-theme-font:minor-bidi;
 +
color:#5A5A5A;
 +
mso-themecolor:text1;
 +
mso-themetint:165;
 +
letter-spacing:.75pt;}
 +
p.msosubtitlecxspfirst, li.msosubtitlecxspfirst, div.msosubtitlecxspfirst
 +
{mso-style-name:msosubtitlecxspfirst;
 +
mso-style-unhide:no;
 +
margin:0cm;
 +
margin-bottom:.0001pt;
 +
line-height:115%;
 +
mso-pagination:widow-orphan;
 +
page-break-after:avoid;
 +
font-size:13.0pt;
 +
font-family:"Trebuchet MS","sans-serif";
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:minor-fareast;
 +
mso-bidi-font-family:"Times New Roman";
 +
color:#666666;
 +
font-style:italic;}
 +
p.msosubtitlecxspmiddle, li.msosubtitlecxspmiddle, div.msosubtitlecxspmiddle
 +
{mso-style-name:msosubtitlecxspmiddle;
 +
mso-style-unhide:no;
 +
margin:0cm;
 +
margin-bottom:.0001pt;
 +
line-height:115%;
 +
mso-pagination:widow-orphan;
 +
page-break-after:avoid;
 +
font-size:13.0pt;
 +
font-family:"Trebuchet MS","sans-serif";
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:minor-fareast;
 +
mso-bidi-font-family:"Times New Roman";
 +
color:#666666;
 +
font-style:italic;}
 +
p.msosubtitlecxsplast, li.msosubtitlecxsplast, div.msosubtitlecxsplast
 +
{mso-style-name:msosubtitlecxsplast;
 +
mso-style-unhide:no;
 +
margin-top:0cm;
 +
margin-right:0cm;
 +
margin-bottom:10.0pt;
 +
margin-left:0cm;
 +
line-height:115%;
 +
mso-pagination:widow-orphan;
 +
page-break-after:avoid;
 +
font-size:13.0pt;
 +
font-family:"Trebuchet MS","sans-serif";
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:minor-fareast;
 +
mso-bidi-font-family:"Times New Roman";
 +
color:#666666;
 +
font-style:italic;}
 +
p.msochpdefault, li.msochpdefault, div.msochpdefault
 +
{mso-style-name:msochpdefault;
 +
mso-style-unhide:no;
 +
mso-margin-top-alt:auto;
 +
margin-right:0cm;
 +
mso-margin-bottom-alt:auto;
 +
margin-left:0cm;
 +
mso-pagination:widow-orphan;
 +
font-size:12.0pt;
 +
font-family:"Arial","sans-serif";
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:minor-fareast;
 +
color:black;}
 +
p.msopapdefault, li.msopapdefault, div.msopapdefault
 +
{mso-style-name:msopapdefault;
 +
mso-style-unhide:no;
 +
mso-margin-top-alt:auto;
 +
margin-right:0cm;
 +
mso-margin-bottom-alt:auto;
 +
margin-left:0cm;
 +
line-height:115%;
 +
mso-pagination:widow-orphan;
 +
font-size:12.0pt;
 +
font-family:"Times New Roman","serif";
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:minor-fareast;}
 +
span.SpellE
 +
{mso-style-name:"";
 +
mso-spl-e:yes;}
 +
span.GramE
 +
{mso-style-name:"";
 +
mso-gram-e:yes;}
 +
.MsoChpDefault
 +
{mso-style-type:export-only;
 +
mso-default-props:yes;
 +
font-size:10.0pt;
 +
mso-ansi-font-size:10.0pt;
 +
mso-bidi-font-size:10.0pt;
 +
font-family:"Arial","sans-serif";
 +
mso-ascii-font-family:Arial;
 +
mso-hansi-font-family:Arial;
 +
mso-bidi-font-family:Arial;
 +
color:black;}
 +
.MsoPapDefault
 +
{mso-style-type:export-only;
 +
line-height:115%;}
 +
@page WordSection1
 +
{size:612.0pt 792.0pt;
 +
margin:72.0pt 72.0pt 72.0pt 72.0pt;
 +
mso-header-margin:35.4pt;
 +
mso-footer-margin:35.4pt;
 +
mso-paper-source:0;}
 +
div.WordSection1
 +
{page:WordSection1;}
 +
-->
 +
</style>
 +
<!--[if gte mso 10]>
 +
<style>
 +
/* Style Definitions */
 +
table.MsoNormalTable
 +
{mso-style-name:"Normal Tablo";
 +
mso-tstyle-rowband-size:0;
 +
mso-tstyle-colband-size:0;
 +
mso-style-noshow:yes;
 +
mso-style-priority:99;
 +
mso-style-parent:"";
 +
mso-padding-alt:0cm 5.4pt 0cm 5.4pt;
 +
mso-para-margin:0cm;
 +
mso-para-margin-bottom:.0001pt;
 +
line-height:115%;
 +
mso-pagination:widow-orphan;
 +
font-size:10.0pt;
 +
font-family:"Arial","sans-serif";
 +
color:black;}
 +
</style>
 +
<![endif]--><!--[if gte mso 9]><xml>
 +
<o:shapedefaults v:ext="edit" spidmax="1034"/>
 +
</xml><![endif]--><!--[if gte mso 9]><xml>
 +
<o:shapelayout v:ext="edit">
 +
  <o:idmap v:ext="edit" data="1"/>
 +
</o:shapelayout></xml><![endif]-->
 +
</head>
 +
 +
<body bgcolor=white lang=TR style='tab-interval:35.4pt'>
 +
 +
<div class=WordSection1>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-size:16.0pt;
 +
line-height:115%;font-family:"Calibri","sans-serif"'>CLONING THE TOP10 STRAINS</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-size:16.0pt;
 +
line-height:115%;font-family:"Calibri","sans-serif"'>&nbsp;</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-size:16.0pt;
 +
line-height:115%;font-family:"Calibri","sans-serif"'>TRANSFORMATION</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'>&nbsp;</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span class=SpellE><span
 +
style='font-family:"Calibri","sans-serif"'>Plasmids</span></span><span
 +
style='font-family:"Calibri","sans-serif"'> <span class=SpellE>containing</span>
 +
<span class=SpellE>the</span> BBa_K1291071 (<span class=SpellE>Horseradish</span>
 +
<span class=SpellE>peroxidase</span> <span class=SpellE>composite</span> <span
 +
class=SpellE>part</span>) <span class=SpellE>and</span> BBa_K1291299 (Lignin <span
 +
class=SpellE>Peroxidase</span> <span class=SpellE>composite</span> <span
 +
class=SpellE>part</span>) <span class=SpellE>parts</span> <span class=SpellE>were</span>
 +
<span class=SpellE>transferred</span> <span class=SpellE>into</span> <span
 +
class=SpellE>our</span> T10 <span class=SpellE>strains</span>, <span
 +
class=SpellE>which</span> <span class=SpellE>were</span> <span class=SpellE>prepared</span>
 +
<span class=SpellE>through</span> <span class=SpellE>the</span> <span
 +
class=SpellE>competent</span> <span class=SpellE>cell</span> <span
 +
class=SpellE>preparation</span> <span class=SpellE>protocol</span>, <span
 +
class=SpellE>using</span> <span class=SpellE>transformation</span> <span
 +
class=SpellE>protocols</span>. <span class=SpellE>Colonies</span> <span
 +
class=SpellE>were</span> <span class=SpellE>seen</span> in <span class=SpellE>agar</span>
 +
<span class=SpellE>plates</span> <span class=SpellE>that</span> had <span
 +
class=SpellE>the</span> <span class=SpellE>appropriate</span> <span
 +
class=SpellE>antibiotics</span> <span class=SpellE>after</span> a 16 <span
 +
class=SpellE>hour</span> <span class=SpellE>long</span> <span class=SpellE>incubation</span>
 +
<span class=SpellE>process</span>. </span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'>&nbsp;</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'>&nbsp;</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-size:16.0pt;
 +
line-height:115%;font-family:"Calibri","sans-serif"'>PLASMID ISOLATION</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'>&nbsp;<span
 +
class=SpellE>The</span> <span class=SpellE>colonies</span> <span class=SpellE>taken</span>
 +
<span class=SpellE>from</span> <span class=SpellE>plates</span> <span
 +
class=SpellE>were</span> <span class=SpellE>incubated</span> in LB <span
 +
class=SpellE>Broth</span> <span class=SpellE>containing</span> AMP <span
 +
class=SpellE>for</span> 16 <span class=SpellE>hours</span>. DNA <span
 +
class=SpellE>isolation</span> <span class=SpellE>was</span> <span class=GramE>done</span>
 +
on <span class=SpellE>the</span> <span class=SpellE>bacteria</span> <span
 +
class=SpellE>culture</span> <span class=SpellE>to</span> <span class=SpellE>check</span>
 +
<span class=SpellE>the</span> <span class=SpellE>efficiency</span>. <span
 +
class=SpellE>The</span> <span class=SpellE>adequacy</span> of <span
 +
class=SpellE>the</span> <span class=SpellE>amount</span> of <span class=SpellE>the</span>
 +
<span class=SpellE>nucleic</span> <span class=SpellE>acid</span> <span
 +
class=SpellE>concentration</span> <span class=SpellE>for</span> <span
 +
class=SpellE>digestion</span> <span class=SpellE>was</span> met in <span
 +
class=SpellE>the</span> <span class=SpellE>acquired</span> <span class=SpellE>samples</span>
 +
<span class=SpellE>and</span> it <span class=SpellE>was</span> <span
 +
class=SpellE>seen</span> <span class=SpellE>that</span> <span class=SpellE>the</span>
 +
<span class=SpellE>samples</span> had <span class=SpellE>peaks</span> at OD
 +
260, <span class=SpellE>which</span> <span class=SpellE>was</span> <span
 +
class=SpellE>captured</span> <span class=SpellE>by</span> <span class=SpellE>the</span>
 +
<span class=SpellE>nanodrop</span>.</span><span style='left:-20px;position:
 +
relative;top:0px;z-index:251658240'> </p>
 +
 +
<table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0
 +
style='mso-cellspacing:0cm;mso-yfti-tbllook:1184;mso-padding-alt:0cm 0cm 0cm 0cm'>
 +
<tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
 +
  <td width=0 style='width:.3pt;padding:0cm 0cm 0cm 0cm'></td>
 +
  <td width=281 style='width:210.75pt;padding:0cm 0cm 0cm 0cm'></td>
 +
  <td width=15 style='width:11.25pt;padding:0cm 0cm 0cm 0cm'></td>
 +
  <td width=290 style='width:217.5pt;padding:0cm 0cm 0cm 0cm'></td>
 +
</tr>
 +
<tr style='mso-yfti-irow:1;mso-yfti-lastrow:yes;height:221.25pt'>
 +
  <td style='padding:0cm 0cm 0cm 0cm;height:221.25pt'></td>
 +
  <td valign=top style='padding:0cm 0cm 0cm 0cm;height:221.25pt'>
 +
  <p class=MsoNormal><!--[if gte vml 1]><v:shapetype id="_x0000_t75"
 +
  coordsize="21600,21600" o:spt="75" o:preferrelative="t" path="m@4@5l@4@11@9@11@9@5xe"
 +
  filled="f" stroked="f">
 +
  <v:stroke joinstyle="miter"/>
 +
  <v:formulas>
 +
    <v:f eqn="if lineDrawn pixelLineWidth 0"/>
 +
    <v:f eqn="sum @0 1 0"/>
 +
    <v:f eqn="sum 0 0 @1"/>
 +
    <v:f eqn="prod @2 1 2"/>
 +
    <v:f eqn="prod @3 21600 pixelWidth"/>
 +
    <v:f eqn="prod @3 21600 pixelHeight"/>
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    <v:f eqn="sum @0 0 1"/>
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    <v:f eqn="prod @6 1 2"/>
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    <v:f eqn="prod @7 21600 pixelWidth"/>
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    <v:f eqn="sum @8 21600 0"/>
 +
    <v:f eqn="prod @7 21600 pixelHeight"/>
 +
    <v:f eqn="sum @10 21600 0"/>
 +
  </v:formulas>
 +
  <v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect"/>
 +
  <o:lock v:ext="edit" aspectratio="t"/>
 +
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 +
  style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif";
 +
  mso-fareast-font-family:"Times New Roman";color:windowtext'><o:p></o:p></span></p>
 +
  </td>
 +
  <td style='padding:0cm 0cm 0cm 0cm;height:221.25pt'></td>
 +
  <td valign=top style='padding:0cm 0cm 0cm 0cm;height:221.25pt'>
 +
  <p class=MsoNormal><!--[if gte vml 1]><v:shape id="_x0000_s1032" type="#_x0000_t75"
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  mso-position-horizontal-relative:text;mso-position-vertical:absolute;
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  mso-position-vertical-relative:text'>
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  <v:imagedata src="https://static.igem.org/mediawiki/2014hs/e/e0/Cimage002.jpg"/>
 +
  <w:wrap type="tight"/>
 +
  </v:shape><![endif]--><![if !vml]><img width=290 height=295
 +
  src="https://static.igem.org/mediawiki/2014hs/e/e0/Cimage002.jpg" align=left
 +
  hspace=12 alt="https://static.igem.org/mediawiki/2014hs/e/e0/Cimage002.jpg" v:shapes="_x0000_s1032"><![endif]><span
 +
  style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif";
 +
  mso-fareast-font-family:"Times New Roman";color:windowtext'><o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
</table>
 +
 +
<p class=MsoNormal style='text-align:justify'><br clear=all style='mso-special-character:
 +
line-break'>
 +
</span><o:p></o:p></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='left:-89px;
 +
position:relative;top:0px;z-index:251660288'><!--[if gte vml 1]><v:shape id="_x0000_s1031"
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type="#_x0000_t75" alt="https://static.igem.org/mediawiki/2014hs/f/f4/Cimage003.jpg"
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mso-wrap-style:square;mso-wrap-distance-left:9pt;mso-wrap-distance-top:0;
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mso-wrap-distance-right:9pt;mso-wrap-distance-bottom:0;
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mso-position-horizontal:absolute;mso-position-horizontal-relative:text;
 +
mso-position-vertical:absolute;mso-position-vertical-relative:text'>
 +
<v:imagedata src="https://static.igem.org/mediawiki/2014hs/f/f4/Cimage003.jpg"/>
 +
<w:wrap type="tight"/>
 +
</v:shape><![endif]--><![if !vml]><img width=761 height=486
 +
src="https://static.igem.org/mediawiki/2014hs/f/f4/Cimage003.jpg" align=left
 +
hspace=12 alt="https://static.igem.org/mediawiki/2014hs/f/f4/Cimage003.jpg" v:shapes="_x0000_s1031"><![endif]><br
 +
clear=all style='mso-special-character:line-break'>
 +
</p>
 +
 +
<p class=MsoNormal style='text-align:justify'><!--[if gte vml 1]><v:shape id="Resim_x0020_2"
 +
o:spid="_x0000_s1030" type="#_x0000_t75" alt="https://static.igem.org/mediawiki/2014hs/9/98/Cimage004.jpg"
 +
style='position:absolute;left:0;text-align:left;margin-left:0;margin-top:0;
 +
width:225pt;height:279pt;z-index:-251658240;visibility:visible;
 +
mso-wrap-style:square;mso-width-percent:0;mso-height-percent:0;
 +
mso-wrap-distance-left:9pt;mso-wrap-distance-top:9pt;
 +
mso-wrap-distance-right:9pt;mso-wrap-distance-bottom:9pt;
 +
mso-position-horizontal:left;mso-position-horizontal-relative:text;
 +
mso-position-vertical:absolute;mso-position-vertical-relative:line;
 +
mso-width-percent:0;mso-height-percent:0;mso-width-relative:page;
 +
mso-height-relative:page' o:allowoverlap="f">
 +
<v:imagedata src="https://static.igem.org/mediawiki/2014hs/9/98/Cimage004.jpg"/>
 +
<w:wrap type="tight" anchory="line"/>
 +
</v:shape><![endif]--><![if !vml]><img width=300 height=372
 +
src="https://static.igem.org/mediawiki/2014hs/9/98/Cimage004.jpg" align=left
 +
hspace=12 vspace=12 alt="https://static.igem.org/mediawiki/2014hs/9/98/Cimage004.jpg"
 +
v:shapes="Resim_x0020_2"><![endif]><span style='font-family:"Calibri","sans-serif"'>&nbsp;</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'>&nbsp;</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span class=SpellE><span
 +
style='font-size:16.0pt;line-height:115%;font-family:"Calibri","sans-serif"'>Digestion</span></span><span
 +
style='font-size:16.0pt;line-height:115%;font-family:"Calibri","sans-serif"'> <span
 +
class=SpellE>and</span> DNA <span class=SpellE>Electrophoresis</span></span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'>&nbsp;</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'>Gel
 +
<span class=SpellE>electrophoresis</span> <span class=SpellE>was</span> <span
 +
class=SpellE>conducted</span> on <span class=SpellE>the</span> <span
 +
class=SpellE>samples</span> <span class=SpellE>so</span> <span class=SpellE>that</span>
 +
<span class=SpellE>the</span> <span class=SpellE>enzymes</span> <span
 +
class=SpellE>which</span> <span class=SpellE>were</span> <span class=SpellE>digested</span>
 +
<span class=SpellE>with</span> <span class=SpellE>the</span> <span
 +
class=SpellE>EcoRI</span> <span class=SpellE>and</span> <span class=SpellE>PstI</span>
 +
<span class=SpellE>could</span> be <span class=SpellE>verified</span> in <span
 +
class=SpellE>terms</span> of <span class=SpellE>their</span> <span
 +
class=SpellE>wavelength</span>. <span class=SpellE>The</span> <span
 +
class=SpellE>acquired</span> <span class=SpellE>results</span> <span
 +
class=SpellE>showed</span> <span class=SpellE>that</span> <span class=SpellE>the</span>
 +
<span class=SpellE>plasmid</span> transfer <span class=SpellE>process</span> <span
 +
class=SpellE>was</span> <span class=SpellE>successful</span> <span
 +
class=SpellE>for</span> <span class=SpellE>the</span> Top10 <span class=SpellE>strains</span>
 +
<span class=SpellE>while</span> <span class=SpellE>the</span> NEB10 <span
 +
class=SpellE>strains</span>, <span class=SpellE>which</span> <span
 +
class=SpellE>were</span> <span class=SpellE>taken</span> <span class=SpellE>from</span>
 +
<span class=SpellE>the</span> <span class=SpellE>stock</span>, <span
 +
class=SpellE>were</span> <span class=SpellE>disfunctional</span> <span
 +
class=SpellE>thus</span> <span class=SpellE>were</span> <span class=SpellE>unusable</span>.</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'>&nbsp;</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'>&nbsp;</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'>&nbsp;</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-size:16.0pt;
 +
line-height:115%;font-family:"Calibri","sans-serif"'>&nbsp;</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-size:16.0pt;
 +
line-height:115%;font-family:"Calibri","sans-serif"'>CLONING THE BL-21 STRAINS</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'>&nbsp;</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'>Since
 +
<span class=SpellE>we</span> <span class=SpellE>require</span> a <span
 +
class=SpellE>high</span> <span class=SpellE>concentration</span> of <span
 +
class=SpellE>proteins</span> in <span class=SpellE>our</span> <span
 +
class=SpellE>project</span>, <span class=SpellE>we</span> <span class=SpellE>decided</span>
 +
<span class=SpellE>to</span> <span class=SpellE>use</span> <span class=SpellE>the</span>
 +
BL-21 <span class=SpellE>strain</span> of <span class=SpellE>E.coli</span> <span
 +
class=SpellE>instead</span> of Top10 <span class=SpellE>strains</span> <span
 +
class=SpellE>due</span> <span class=SpellE>to</span> <span class=SpellE>its</span>
 +
<span class=SpellE>enhanced</span> protein <span class=SpellE>synthesis</span> <span
 +
class=SpellE>capacity</span>. <span class=SpellE>Plasmids</span> <span
 +
class=SpellE>containing</span> <span class=SpellE>the</span> BBa_K1291071 (<span
 +
class=SpellE>Horseradish</span> <span class=SpellE>peroxidase</span> <span
 +
class=SpellE>composite</span> <span class=SpellE>part</span>) <span
 +
class=SpellE>and</span> BBa_K1291299 (Lignin <span class=SpellE>Peroxidase</span>
 +
<span class=SpellE>composite</span> <span class=SpellE>part</span>) <span
 +
class=SpellE>parts</span> <span class=SpellE>were</span> <span class=SpellE>transferred</span>
 +
<span class=SpellE>into</span> <span class=SpellE>our</span> BL-21 <span
 +
class=SpellE>strains</span>, <span class=SpellE>which</span> <span
 +
class=SpellE>were</span> <span class=SpellE>prepared</span> <span class=SpellE>through</span>
 +
<span class=SpellE>the</span> <span class=SpellE>competent</span> <span
 +
class=SpellE>cell</span> <span class=SpellE>preparation</span> <span
 +
class=SpellE>protocol</span>, <span class=SpellE>using</span> <span
 +
class=SpellE>the</span> <span class=SpellE>transformation</span> <span
 +
class=SpellE>protocol</span>. <span class=SpellE>The</span> <span class=SpellE>colonies</span>
 +
<span class=SpellE>successfully</span> <span class=SpellE>acquired</span> <span
 +
class=SpellE>after</span> a 16-hour <span class=SpellE>long</span> <span
 +
class=SpellE>incubation</span> <span class=SpellE>process</span>.</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'>&nbsp;</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'>&nbsp;</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'>&nbsp;</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-size:16.0pt;
 +
mso-bidi-font-size:11.0pt;line-height:115%;font-family:"Calibri","sans-serif"'>PLASMID
 +
ISOLATION</span><span style='left:-20px;position:relative;top:0px;z-index:251662336'><span
 +
style='font-size:16.0pt;mso-bidi-font-size:11.0pt;line-height:115%'> <o:p></o:p></span></p>
 +
 +
<table class=MsoNormalTable border=0 cellspacing=0 cellpadding=0
 +
style='mso-cellspacing:0cm;mso-yfti-tbllook:1184;mso-padding-alt:0cm 0cm 0cm 0cm'>
 +
<tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes'>
 +
  <td width=0 style='width:.3pt;padding:0cm 0cm 0cm 0cm'></td>
 +
  <td width=309 style='width:231.75pt;padding:0cm 0cm 0cm 0cm'></td>
 +
  <td width=34 style='width:25.5pt;padding:0cm 0cm 0cm 0cm'></td>
 +
  <td width=281 style='width:210.75pt;padding:0cm 0cm 0cm 0cm'></td>
 +
</tr>
 +
<tr style='mso-yfti-irow:1;height:230.25pt'>
 +
  <td style='padding:0cm 0cm 0cm 0cm;height:230.25pt'></td>
 +
  <td rowspan=2 valign=top style='padding:0cm 0cm 0cm 0cm;height:230.25pt'>
 +
  <p class=MsoNormal><!--[if gte vml 1]><v:shape id="Resim_x0020_4" o:spid="_x0000_s1029"
 +
  type="#_x0000_t75" alt="https://static.igem.org/mediawiki/2014hs/1/1b/Cimage005.jpg"
 +
  style='position:absolute;margin-left:0;margin-top:0;width:231.75pt;height:231.75pt;
 +
  z-index:-251653120;visibility:visible;mso-wrap-style:square;
 +
  mso-wrap-distance-left:9pt;mso-wrap-distance-top:0;
 +
  mso-wrap-distance-right:9pt;mso-wrap-distance-bottom:0;
 +
  mso-position-horizontal:absolute;mso-position-horizontal-relative:text;
 +
  mso-position-vertical:absolute;mso-position-vertical-relative:text'>
 +
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  </v:shape><![endif]--><![if !vml]><img width=309 height=309
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  <w:wrap type="tight"/>
 +
  </v:shape><![endif]--><![if !vml]><img width=281 height=307
 +
  src="https://static.igem.org/mediawiki/2014hs/1/1f/Cimage006.jpg" align=left
 +
  hspace=12 alt="https://static.igem.org/mediawiki/2014hs/1/1f/Cimage006.jpg" v:shapes="Resim_x0020_5"><![endif]><span
 +
  style='font-size:12.0pt;line-height:115%;font-family:"Times New Roman","serif";
 +
  mso-fareast-font-family:"Times New Roman";color:windowtext'><o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:2;mso-yfti-lastrow:yes;height:1.5pt'>
 +
  <td style='padding:0cm 0cm 0cm 0cm;height:1.5pt'></td>
 +
  <td style='padding:0cm 0cm 0cm 0cm;height:1.5pt'></td>
 +
  <td style='padding:0cm 0cm 0cm 0cm;height:1.5pt'></td>
 +
</tr>
 +
</table>
 +
 +
<p class=MsoNormal style='text-align:justify'><br clear=all style='mso-special-character:
 +
line-break'>
 +
</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><!--[if gte vml 1]><v:shape id="Resim_x0020_6"
 +
o:spid="_x0000_s1027" type="#_x0000_t75" alt="https://static.igem.org/mediawiki/2014hs/5/55/Cimage007.gif"
 +
style='position:absolute;left:0;text-align:left;margin-left:0;margin-top:1442.45pt;
 +
width:567.75pt;height:362.25pt;z-index:-251651072;visibility:visible;
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mso-position-vertical:absolute;mso-position-vertical-relative:text'>
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<v:imagedata src="https://static.igem.org/mediawiki/2014hs/5/55/Cimage007.gif"/>
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<w:wrap type="tight"/>
 +
</v:shape><![endif]--><![if !vml]><img width=757 height=483
 +
src="https://static.igem.org/mediawiki/2014hs/5/55/Cimage007.gif" align=left
 +
hspace=12 alt="https://static.igem.org/mediawiki/2014hs/5/55/Cimage007.gif" v:shapes="Resim_x0020_6"><![endif]><span
 +
style='font-family:"Calibri","sans-serif"'><span
 +
style='mso-spacerun:yes'>                                                                                                                                                                                                          
 +
</span><span style='mso-spacerun:yes'>                     </span><span
 +
style='mso-spacerun:yes'>          </span><span class=SpellE>The</span> <span
 +
class=SpellE>chosen</span> <span class=SpellE>colonies</span> <span
 +
class=SpellE>were</span> <span class=SpellE>incubated</span> <span
 +
class=SpellE>for</span> 16-hours in AMP <span class=SpellE>containing</span> LB
 +
<span class=SpellE>Broth</span>. DNA <span class=SpellE>Isolation</span> <span
 +
class=SpellE>was</span> <span class=GramE>done</span> <span class=SpellE>to</span>
 +
test <span class=SpellE>the</span> <span class=SpellE>efficiency</span> of <span
 +
class=SpellE>the</span> <span class=SpellE>transformation</span>. A <span
 +
class=SpellE>peek</span> at OD 260 <span class=SpellE>was</span> <span
 +
class=SpellE>observed</span> <span class=SpellE>and</span> <span class=SpellE>sufficient</span>
 +
<span class=SpellE>levels</span> of DNA <span class=SpellE>concentration</span>
 +
<span class=SpellE>was</span> <span class=SpellE>acquired</span> <span
 +
class=SpellE>to</span> <span class=SpellE>continue</span> <span class=SpellE>to</span>
 +
<span class=SpellE>digestion</span>.</span><span style='left:-78px;position:
 +
relative;top:0px;z-index:251664384'> <br clear=all style='mso-special-character:
 +
line-break'>
 +
<span style='font-family:"Calibri","sans-serif"'><o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'>&nbsp;</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><!--[if gte vml 1]><v:shape id="Resim_x0020_3"
 +
o:spid="_x0000_s1026" type="#_x0000_t75" alt="https://static.igem.org/mediawiki/2014hs/c/cf/Cimage008.jpg"
 +
style='position:absolute;left:0;text-align:left;margin-left:0;margin-top:0;
 +
width:255pt;height:267.75pt;z-index:-251657216;visibility:visible;
 +
mso-wrap-style:square;mso-width-percent:0;mso-height-percent:0;
 +
mso-wrap-distance-left:9pt;mso-wrap-distance-top:9pt;
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mso-width-percent:0;mso-height-percent:0;mso-width-relative:page;
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mso-height-relative:page' o:allowoverlap="f">
 +
<v:imagedata src="https://static.igem.org/mediawiki/2014hs/c/cf/Cimage008.jpg"/>
 +
<w:wrap type="tight" anchory="line"/>
 +
</v:shape><![endif]--><![if !vml]><img width=340 height=357
 +
src="https://static.igem.org/mediawiki/2014hs/c/cf/Cimage008.jpg" align=left
 +
hspace=12 vspace=12 alt="https://static.igem.org/mediawiki/2014hs/c/cf/Cimage008.jpg"
 +
v:shapes="Resim_x0020_3"><![endif]><span class=SpellE><span style='font-size:
 +
16.0pt;line-height:115%;font-family:"Calibri","sans-serif"'>Digestion</span></span><span
 +
style='font-size:16.0pt;line-height:115%;font-family:"Calibri","sans-serif"'> <span
 +
class=SpellE>and</span> DNA <span class=SpellE>Electrophoresis</span></span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'>&nbsp;</span></p>
 +
 +
<p class=MsoNormal style='text-align:justify'><span style='font-family:"Calibri","sans-serif"'>Gel
 +
<span class=SpellE>electrophoresis</span> <span class=SpellE>was</span> <span
 +
class=SpellE>conducted</span> on <span class=SpellE>the</span> <span
 +
class=SpellE>samples</span> <span class=SpellE>so</span> <span class=SpellE>that</span>
 +
<span class=SpellE>the</span> <span class=SpellE>enzymes</span> <span
 +
class=SpellE>which</span> <span class=SpellE>were</span> <span class=SpellE>digested</span>
 +
<span class=SpellE>with</span> <span class=SpellE>the</span> <span
 +
class=SpellE>EcoRI</span> <span class=SpellE>and</span> <span class=SpellE>PstI</span>
 +
<span class=SpellE>could</span> be <span class=SpellE>verified</span> in <span
 +
class=SpellE>terms</span> of <span class=SpellE>their</span> <span
 +
class=SpellE>wavelength</span>. <span class=SpellE>The</span> <span
 +
class=SpellE>acquired</span> <span class=SpellE>results</span> <span
 +
class=SpellE>showed</span> <span class=SpellE>that</span> <span class=SpellE>the</span>
 +
<span class=SpellE>plasmid</span> transfer <span class=SpellE>process</span> <span
 +
class=SpellE>was</span> <span class=SpellE>successful</span> <span
 +
class=SpellE>for</span> BL-21 <span class=SpellE>Strains</span> <span
 +
class=SpellE>and</span> it <span class=SpellE>was</span> <span class=SpellE>possible</span>
 +
<span class=SpellE>to</span> <span class=SpellE>continue</span> <span
 +
class=SpellE>to</span> <span class=SpellE>the</span> <span class=SpellE>next</span>
 +
step.</span></p>
 +
 +
</div>
 +
 +
</body>
 +
 +
</head>
 +
</html>

Latest revision as of 17:35, 26 April 2015

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CLONING THE TOP10 STRAINS

 

TRANSFORMATION

 

Plasmids containing the BBa_K1291071 (Horseradish peroxidase composite part) and BBa_K1291299 (Lignin Peroxidase composite part) parts were transferred into our T10 strains, which were prepared through the competent cell preparation protocol, using transformation protocols. Colonies were seen in agar plates that had the appropriate antibiotics after a 16 hour long incubation process.

 

 

PLASMID ISOLATION

 The colonies taken from plates were incubated in LB Broth containing AMP for 16 hours. DNA isolation was done on the bacteria culture to check the efficiency. The adequacy of the amount of the nucleic acid concentration for digestion was met in the acquired samples and it was seen that the samples had peaks at OD 260, which was captured by the nanodrop.

https://static.igem.org/mediawiki/2014hs/a/a8/Cimage001.jpg

https://static.igem.org/mediawiki/2014hs/e/e0/Cimage002.jpg


https://static.igem.org/mediawiki/2014hs/f/f4/Cimage003.jpg

https://static.igem.org/mediawiki/2014hs/9/98/Cimage004.jpg 

 

Digestion and DNA Electrophoresis

 

Gel electrophoresis was conducted on the samples so that the enzymes which were digested with the EcoRI and PstI could be verified in terms of their wavelength. The acquired results showed that the plasmid transfer process was successful for the Top10 strains while the NEB10 strains, which were taken from the stock, were disfunctional thus were unusable.

 

 

 

 

CLONING THE BL-21 STRAINS

 

Since we require a high concentration of proteins in our project, we decided to use the BL-21 strain of E.coli instead of Top10 strains due to its enhanced protein synthesis capacity. Plasmids containing the BBa_K1291071 (Horseradish peroxidase composite part) and BBa_K1291299 (Lignin Peroxidase composite part) parts were transferred into our BL-21 strains, which were prepared through the competent cell preparation protocol, using the transformation protocol. The colonies successfully acquired after a 16-hour long incubation process.

 

 

 

PLASMID ISOLATION

https://static.igem.org/mediawiki/2014hs/1/1b/Cimage005.jpg

https://static.igem.org/mediawiki/2014hs/1/1f/Cimage006.jpg


https://static.igem.org/mediawiki/2014hs/5/55/Cimage007.gif                                                                                                                                                                                                                                          The chosen colonies were incubated for 16-hours in AMP containing LB Broth. DNA Isolation was done to test the efficiency of the transformation. A peek at OD 260 was observed and sufficient levels of DNA concentration was acquired to continue to digestion.

 

https://static.igem.org/mediawiki/2014hs/c/cf/Cimage008.jpgDigestion and DNA Electrophoresis

 

Gel electrophoresis was conducted on the samples so that the enzymes which were digested with the EcoRI and PstI could be verified in terms of their wavelength. The acquired results showed that the plasmid transfer process was successful for BL-21 Strains and it was possible to continue to the next step.

Retrieved from "http://2014hs.igem.org/Team:AUC_TURKEY/Results/Cloning"