Team:AUC TURKEY/Project/Parts

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-
  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2"/>
 
-
  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3"/>
 
-
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-
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-
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-
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid"/>
 
-
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-
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-
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-
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-
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-
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-
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-
  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 3"/>
 
-
  <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 3"/>
 
-
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 3"/>
 
-
  <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 4"/>
 
-
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-
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-
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-
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-
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-
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-
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-
  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 4"/>
 
-
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-
  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 4"/>
 
-
  <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 4"/>
 
-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 6"/>
 
-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
  <w:LsdException Locked="false" Priority="46" Name="List Table 1 Light"/>
 
-
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-
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-
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-
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-
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-
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-
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-
  Name="List Table 1 Light Accent 1"/>
 
-
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-
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-
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-
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-
  Name="List Table 6 Colorful Accent 1"/>
 
-
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-
  Name="List Table 7 Colorful Accent 1"/>
 
-
  <w:LsdException Locked="false" Priority="46"
 
-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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-
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{mso-style-name:msochpdefault;
 
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mso-bidi-font-family:"Times New Roman";}
 
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{mso-style-name:msopapdefault;
 
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-
{page:WordSection1;}
 
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<style>
 
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/* Style Definitions */
 
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table.MsoNormalTable
 
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font-family:"Calibri","sans-serif";}
 
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-
</head>
 
-
 
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-
 
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<div class=WordSection1>
 
-
 
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<p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>&nbsp;</span></p>
 
-
 
-
<p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>&nbsp;</span></p>
 
-
 
-
<p class=MsoNormal><span lang=EN-US style='font-size:16.0pt;mso-bidi-font-size:
 
-
11.0pt;line-height:115%;mso-ansi-language:EN-US'>Part Design:</span><span
 
-
style='font-size:16.0pt;mso-bidi-font-size:11.0pt;line-height:115%'><o:p></o:p></span></p>
 
-
 
-
<p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>In our
 
-
project there are 4 different parts. Two of them composite parts that are
 
-
functional and other 2 parts are our enzymes that are proteins. We designed our
 
-
parts with Gene Designer 2.0 program. We found our enzyme sequences from NCBI
 
-
PubMed Database. In our project we need that our proteins to be produced and we
 
-
need our enzymes so much because they do the all work. For more production we
 
-
chose the strongest promoter there is which the strongest constitutive promoter
 
-
J23119 in Part Registry is. When we design our parts we received this
 
-
information that; scientists use inducible promoters for protein
 
-
over-production so in our design, right after the promoter and also in front of
 
-
our RBS, we added <span class=SpellE>BamHI</span> restriction enzyme site.
 
-
After the RBS we added our enzymes sequences HRP-C and Lip-1 and after the
 
-
enzymes sequences we added His-Tag <span class=GramE>( 6</span> Histidine
 
-
sequences). Because we planned to do Western-Blotting technique in order to
 
-
show that our proteins are produced successfully. &nbsp;</span></p>
 
-
 
-
<p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>&nbsp;</span></p>
 
-
 
-
<p class=MsoListParagraph style='text-indent:-18.0pt'><span lang=EN-US
 
-
style='font-size:16.0pt;line-height:115%;mso-ansi-language:EN-US'>1)</span><span
 
-
lang=EN-US style='font-size:7.0pt;line-height:115%;font-family:"Times New Roman","serif";
 
-
mso-ansi-language:EN-US'>&nbsp;&nbsp;&nbsp; </span><span lang=EN-US
 
-
style='font-size:16.0pt;line-height:115%;mso-ansi-language:EN-US'>Horseradish
 
-
Peroxidase-C Part Design:</span></p>
 
-
 
-
<p class=MsoListParagraph><span lang=EN-US style='mso-ansi-language:EN-US'>&nbsp;</span></p>
 
-
 
-
<p class=MsoListParagraph><span style='mso-no-proof:yes'><img width=653
 
-
height=290 id="_x0000_i1026"
 
-
src="https://static.igem.org/mediawiki/2014hs/e/e7/1image001.png"
 
-
alt="https://static.igem.org/mediawiki/2014hs/e/e7/1image001.png"></span></p>
 
-
 
-
<p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>&nbsp;</span></p>
 
-
 
-
<p class=MsoListParagraph style='text-indent:-18.0pt'><span lang=EN-US
 
-
style='font-size:16.0pt;line-height:115%;mso-ansi-language:EN-US'>2)</span><span
 
-
lang=EN-US style='font-size:7.0pt;line-height:115%;font-family:"Times New Roman","serif";
 
-
mso-ansi-language:EN-US'>&nbsp;&nbsp;&nbsp; </span><span lang=EN-US
 
-
style='font-size:16.0pt;line-height:115%;mso-ansi-language:EN-US'>Lignin Peroxidase-1<span
 
-
class=GramE>&nbsp; Part</span> Design : </span></p>
 
-
 
-
<p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
-
</span><span style='mso-no-proof:yes'><img width=665 height=277
 
-
id="_x0000_i1025" src="https://static.igem.org/mediawiki/2014hs/3/32/2image002.png"
 
-
alt="https://static.igem.org/mediawiki/2014hs/3/32/2image002.png"></span></p>
 
-
 
-
<p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>&nbsp;</span></p>
 
-
 
-
</div>
 
-
 
-
</body>
 
-
 
-
 
-
 
-
</head>
 
-
</html>
 

Revision as of 21:42, 18 February 2015