Team:AUC TURKEY/Notebook/Safety

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'>
 
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<h2 style='margin-top:0cm;margin-right:0cm;margin-bottom:7.2pt;margin-left:
 
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0cm; ;border:none;padding:0cm'><span class=mw-headline><span
 
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style='font-size:16.0pt;font-family:"Calibri","sans-serif";font-weight:normal'>Before
 
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we do</span></span></h2>
 
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</div>
 
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<p style='margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:
 
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0cm;line-height:14.3pt; '><span style='font-family:"Calibri","sans-serif"'>Personel
 
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safety is one of the top priorities in the lab. Before starting lab work, we
 
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attended a lab safety training program provided by our teacher Mr.Bestepe. We
 
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were familiarized with the operation of lab equipment and nature of chemicals
 
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we would be using in the lab.</span></p>
 
-
 
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<p style='margin:0cm;margin-bottom:.0001pt;text-align:justify;line-height:15.75pt;
 
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'><span style='font-family:"Calibri","sans-serif";0'>We used our team funds to buy disinfectant white medical uniforms.
 
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Before we enter the lab, everyone must put on the uniform and go through the disinfectant
 
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door to make sure that they are clean. Some chemicals may be harmful to human
 
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skin, so everyone is required to wear safety gloves, to isolate the reagents
 
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from our skin. When important and strict steps are required, we have to spray
 
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the alcohol on our gloves to be sure that our gloves are cleaned. These are
 
-
universal precautionary measures.</span></p>
 
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<p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>&nbsp;</span></p>
 
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<div style='border:none;border-bottom:solid #AAAAAA 1.0pt;padding:0cm 0cm 2.0pt 0cm'>
 
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<p class=MsoNormal style='margin-bottom:7.2pt;border:none;padding:0cm'><span
 
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style='font-size:16.0pt; '>1. Would any of your project ideas
 
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raise safety issues in terms of researcher safety, public safety, environmental
 
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or ethical safety?</span></p>
 
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</div>
 
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<p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>a.<span
 
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class=apple-converted-space>&nbsp;</span><b>Reseacher safety</b>: <br>
 
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Everyone on the team obtained laboratory safety training through the Machinery
 
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of Life/Foundations of Molecular Medicine and Bioengineering CAPS Class and had
 
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experience with bacterial culture (in E.coli) and genetic transformation
 
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(inserting pGLO into E.coli) prior to beginning the project.</span></p>
 
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<p class=MsoNormal><b><span style='font-size:12.0pt;line-height:115%'>During
 
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the work, our team took the following safety precautions:</span></b></p>
 
-
 
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<p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>Lab coats,
 
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gloves and goggles were worn at all times.</span></p>
 
-
 
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<p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>All
 
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equipment (both disposable and autoclaved) was sterile.</span></p>
 
-
 
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<p class=MsoNormal style='margin-bottom:1.2pt;margin-left:38.4pt;text-indent:
 
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-18.0pt;line-height:14.65pt'><span style='font-size:12.0pt'>1.<span
 
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style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp; </span></span><span
 
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style='font-size:12.0pt'>The bench and other work surfaces were sterilized with
 
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water and bleach before and after lab work.</span></p>
 
-
 
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<p class=MsoNormal style='margin-bottom:1.2pt;margin-left:38.4pt;text-indent:
 
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-18.0pt;line-height:14.65pt'><span style='font-size:12.0pt'>2.<span
 
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style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp; </span></span><span
 
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style='font-size:12.0pt'>Because cyanobacteria can pose a risk if ingested,
 
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there was no food or drink in the lab.</span></p>
 
-
 
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<p class=MsoNormal style='margin-bottom:1.2pt;margin-left:18.0pt;line-height:
 
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14.3pt'><span style='font-size:12.0pt'>There were no lit burners or other fire
 
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during lab work.</span></p>
 
-
 
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<p style='margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:
 
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0cm;line-height:14.3pt'><span style='font-family:"Calibri","sans-serif"'>b.<span
 
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class=apple-converted-space>&nbsp;</span><b>Public Safety</b>: When released by
 
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accident, our parts and materials actually cause no damage to the general
 
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public. Due to the unability of E.coli strains, TOP10 and BL21 to survive out
 
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of the lab, they cannot pose any risk to the safety and health of the general
 
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public.</span></p>
 
-
 
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<p style='margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:
 
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0cm;line-height:14.3pt'><span style='font-family:"Calibri","sans-serif"'>c.<span
 
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class=apple-converted-space>&nbsp;</span><b>Environment</b>: E. coli strains TOP10
 
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and BL21 have very limited ability to survive outside the laboratory; so that,
 
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it would be unable to survive or disseminate. Therefore, there is no specific
 
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environmental risk associated with the E. coli strains. All bacterial wastes
 
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are kept in 10% bleaching solution for one day, and then, are autoclaved to be
 
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sterilized. Yet, undesired GMOs may achieve ecologically harmful features.</span></p>
 
-
 
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<p style='margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:
 
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0cm;line-height:14.3pt'><b><span style='font-family:"Calibri","sans-serif"'>d.Ethical
 
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Safety</span></b></p>
 
-
 
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<p style='margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:
 
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0cm;line-height:14.3pt'><span style='font-family:"Calibri","sans-serif"'>We
 
-
recognize that our Lignin Peroksydase gene was isolated by Mr.Bestepe and that
 
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we only get use it per his permission. Accordingly, we have kept him informed
 
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on our work with it and the possibility of this gene becoming readily avaliable
 
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in the Standard Registry of Biological Parts.</span></p>
 
-
 
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<div style='border:none;border-bottom:solid #AAAAAA 1.0pt;padding:0cm 0cm 2.0pt 0cm'>
 
-
 
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<p class=MsoNormal style='margin-bottom:7.2pt;border:none;padding:0cm'><span
 
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style='font-size:16.0pt; '>2. Do any of the new BioBrick parts
 
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(or devices) that you made this year raise safety issues?</span></p>
 
-
 
-
</div>
 
-
 
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<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
 
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14.3pt'><span style='font-size:12.0pt; '>No, none of the
 
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BioBricks that were made by us this year raise any safety issues. They are a
 
-
100% safe for the environment, general public, and our team members handling
 
-
them.&nbsp;</span></p>
 
-
 
-
<p style='margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:
 
-
0cm;line-height:14.3pt'><span style='font-family:"Calibri","sans-serif"'>Lignin
 
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Peroksydase</span><span class=apple-converted-space><span style='font-family:
 
-
"Calibri","sans-serif"'>&nbsp;</span></span><span style='font-family:"Calibri","sans-serif"'>is
 
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a comletely safe and common enzyme. Being in many organisms and commonly used
 
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in many applications, Lignin Peroksydase is still considered one of the safest
 
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enzymes. The DegradEcolor is nothing but an mRNA sequence which works as a
 
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simple DegradEcolors in organisms. The DegradEcolor won't even have the
 
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slightest effect on any external being.</span></p>
 
-
 
-
<div style='border:none;border-bottom:solid #AAAAAA 1.0pt;padding:0cm 0cm 2.0pt 0cm'>
 
-
 
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<p class=MsoNormal style='margin-bottom:7.2pt;border:none;padding:0cm'><span
 
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style='font-size:16.0pt; '>3. Is there a local biosafety group,
 
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committee, or review board at your institution?</span></p>
 
-
 
-
</div>
 
-
 
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<p style='margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:
 
-
0cm;line-height:14.3pt'><span style='font-family:"Calibri","sans-serif"'>Our
 
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institution has its own biosafety rules. Rules for laboratory use, general principles,
 
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prevention from hazardous materials and application of emergency intervention
 
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in case of accident are included. In Turgut Ozal University Medical School,
 
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Laboratory and Patient-Employee Safety Committee is responsible for control as
 
-
well as biosafety of laboratories and safety of patients and employees. This
 
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committee works under one of the vice medical director of Turgut Ozal
 
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University Hospital, Prof. Dr. Mehmet Gunduz. Safety and security issues are
 
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found sufficient enough that no change is considered as necessary.<span
 
-
class=apple-converted-space>&nbsp;</span><i>(Further questions can be directed
 
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to Prof. Gunduz, tel: +90-312-203 5103, mgunduz@fatih.edu.tr)</i><span
 
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class=apple-converted-space>&nbsp;</span>Our advisor provided us with biosafety
 
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and lab training before starting our project. In the training, general safety
 
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rules of laboratory use, prevention from hazardous chemicals and solutions as
 
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well as emergency intervention in case of accident were included such as:</span></p>
 
-
 
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<p class=MsoNormal style='margin-bottom:1.2pt;margin-left:18.0pt;text-indent:
 
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-18.0pt;line-height:14.3pt'><span style='font-size:10.0pt;font-family:Wingdings'>§<span
 
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style='font:7.0pt "Times New Roman"'>&nbsp; </span></span><span
 
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style='font-size:12.0pt'>Eating, drinking, storing food and smoking are
 
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absolutely not allowed.</span></p>
 
-
 
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<p class=MsoNormal style='margin-bottom:1.2pt;margin-left:18.0pt;text-indent:
 
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-18.0pt;line-height:14.3pt'><span style='font-size:10.0pt;font-family:Wingdings'>§<span
 
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style='font:7.0pt "Times New Roman"'>&nbsp; </span></span><span
 
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style='font-size:12.0pt'>Mouth pipetting is not allowed; modern pipettes are used
 
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in lab.</span></p>
 
-
 
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<p class=MsoNormal style='margin-bottom:1.2pt;margin-left:18.0pt;text-indent:
 
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-18.0pt;line-height:14.3pt'><span style='font-size:10.0pt;font-family:Wingdings'>§<span
 
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style='font:7.0pt "Times New Roman"'>&nbsp; </span></span><span
 
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style='font-size:12.0pt'>Hazardous wastes and ordinary wastes are separated and
 
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cautiously disposed.</span></p>
 
-
 
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<p class=MsoNormal style='margin-bottom:1.2pt;margin-left:18.0pt;text-indent:
 
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-18.0pt;line-height:14.3pt'><span style='font-size:10.0pt;font-family:Wingdings'>§<span
 
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style='font:7.0pt "Times New Roman"'>&nbsp; </span></span><span
 
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style='font-size:12.0pt'>Lab coats are obligatory to wear in the lab and during
 
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on-going experiments.</span></p>
 
-
 
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<p class=MsoNormal style='margin-bottom:1.2pt;margin-left:18.0pt;text-indent:
 
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-18.0pt;line-height:14.3pt'><span style='font-size:10.0pt;font-family:Wingdings'>§<span
 
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style='font:7.0pt "Times New Roman"'>&nbsp; </span></span><span
 
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style='font-size:12.0pt'>In electrophoresis room, lab coat, protective eyewear,
 
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lab masks and gloves have to be worn. In the case of use of<span
 
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class=apple-converted-space>&nbsp;</span><b>EtBr</b>; extra caution is
 
-
required.</span></p>
 
-
 
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<p class=MsoNormal style='margin-bottom:1.2pt;margin-left:18.0pt;text-indent:
 
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-18.0pt;line-height:14.3pt'><span style='font-size:10.0pt;font-family:Wingdings'>§<span
 
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style='font:7.0pt "Times New Roman"'>&nbsp; </span></span><span
 
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style='font-size:12.0pt'>Washing hands after any experiment and after touching
 
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anything related with viable material is obliged.</span></p>
 
-
 
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<p class=MsoNormal style='margin-bottom:1.2pt;margin-left:18.0pt;text-indent:
 
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-18.0pt;line-height:14.3pt'><span style='font-size:10.0pt;font-family:Wingdings'>§<span
 
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style='font:7.0pt "Times New Roman"'>&nbsp; </span></span><span
 
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style='font-size:12.0pt'>Air conditioning is kept closed during on-going
 
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experiments in order to avoid possible infections of spores and bacteria.</span></p>
 
-
 
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<p class=MsoNormal style='margin-bottom:1.2pt;line-height:14.3pt'><span
 
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style='font-size:12.0pt'>&nbsp;</span></p>
 
-
 
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<div style='border:none;border-bottom:solid #AAAAAA 1.0pt;padding:0cm 0cm 2.0pt 0cm'>
 
-
 
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<p class=MsoNormal style='margin-bottom:7.2pt;border:none;padding:0cm'><span
 
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style='font-size:16.0pt; '>4. Do you have any other ideas how to
 
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deal with safety issues that could be useful for future iGEM competitions? How
 
-
could parts, devices and systems be made even safer through biosafety
 
-
engineering?</span></p>
 
-
 
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</div>
 
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<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
 
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normal'><span style='font-size:12.0pt; '><br>
 
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To deal with safety issues, for future iGEM competition, all Biobrick part
 
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should contian a potential knockout device. Also, Biobrick parts can be
 
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screened for potentially dangerous pathogens through the use of a variety of
 
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software that can be found at universities like Turgut Ozal UNİVERİTY Medical
 
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School.</span></p>
 
-
 
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<p class=MsoNormal style='margin-bottom:0cm;margin-bottom:.0001pt;line-height:
 
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normal'><span style='font-size:12.0pt'>&nbsp;</span></p>
 
-
 
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<div style='border:none;border-bottom:solid #AAAAAA 1.0pt;padding:0cm 0cm 2.0pt 0cm;
 
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'>
 
-
 
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<h2 style='margin-top:0cm;margin-right:0cm;margin-bottom:7.2pt;margin-left:
 
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0cm; ;border:none;padding:0cm'><span class=mw-headline><span
 
-
style='font-size:16.0pt;font-family:"Calibri","sans-serif";font-weight:normal'>Something
 
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dangerous</span></span></h2>
 
-
 
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</div>
 
-
 
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<p style='margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:
 
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0cm;line-height:14.3pt; '><span style='font-family:"Calibri","sans-serif"'>When
 
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we approach the operating floor, especially doing the electrophoresis (to
 
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separate the different part of the plasmid) and usually the gel we used was
 
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made of carcinogen. Therefore, when students are doing this step, we mustn’t
 
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touch the floor, even can’t get very close to it. Another harmful thing is the
 
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ultraviolet rays. Because the rays which used by lab’s equipment have strong
 
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radiation, so we can not obverse it directly, we have to use an idiosyncratic
 
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board to see it, in order to reduce the harm for our eyes.</span></p>
 
-
 
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<p class=MsoNormal style='margin-bottom:1.2pt;margin-left:18.0pt;line-height:
 
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14.3pt'><span style='font-size:12.0pt'>&nbsp;</span></p>
 
-
 
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<p style='margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:
 
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0cm;line-height:14.3pt'><span style='font-family:"Calibri","sans-serif"'>Turkey
 
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has national biosafety regulations and the link is given as<i>(</i></span><span
 
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style='font-family:"Calibri","sans-serif"'><a
 
-
href="http://www.tbbdm.gov.tr/en/Home/BioSafetyCouncilHome/BioSafetyCouncilHomeChoose.aspx"><i><span
 
-
style='color:windowtext'>http://www.tbbdm.gov.tr/en/Home/BioSafetyCouncilHome/BioSafetyCouncilHomeChoose.aspx</span></i></a></span><i><span
 
-
style='font-family:"Calibri","sans-serif"'>)</span></i><span style='font-family:
 
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"Calibri","sans-serif"'>.</span></p>
 
-
 
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<p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>&nbsp;</span></p>
 
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<p class=MsoNormal><span style='font-size:12.0pt;line-height:115%'>&nbsp;</span></p>
 
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Revision as of 21:43, 18 February 2015