Team:AUC TURKEY

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Revision as of 19:07, 12 March 2014

You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.	File:AUC TURKEY logo.png 200px
Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)	File:AUC TURKEY team.png Your team picture
	Team AUC_TURKEY

Official Team Profile

Our project this year will be the continuation of our last year's project. In 'BactoCooler 2.0' we shall make major improvements to the project itself to allow it to reach the state in which it can be effectively applied in real life as a product. After the analysis of the functions of the project last year, we discovered three main problems. The problems with their solutions are as follows: 1)There are safety issues with BactoCooler being released into the environment and specifically into the air. Through installing Kill-Switch mechanisms designed to activate on air contact, we can remove concerns of contamination. 2)BactoCooler synthesizes the urease enzyme which catalyzes the breakdown of the urea in the environment eventually leading to the accumulation of ammonia. Ammonia, being a basic compound, raises the pH of the environment after accumulating and causes alkalization. The highly basic environment results in the death of BactoCooler. To counteract the issue, a pH sensitive system which will detect the alkaline conditions will allow the secretion of an acidic buffer protein will be integrated in 'BactoCooler 2.0'. 3)The time required for over-night incubation is much too long making immediate effect impossible to reach. Aside from the long term effect, due to the nature of our project, we the switch system regulated by the RNA Thermometer cannot be actively used if a short term response is not achieved. We have conducted research to find possible solutions on the matter and found some possible solutions that suit our project from the work of previous iGEM projects. Different protein expression experiments such as the Western-Blot, SDS-Page and ELISA can serve our purpose. Alongside the application of the possible solutions to 'BactoCooler 2.0', we are planning to do modelling to inspect the real-life application of our project. As AUC_TURKEY we would like to state our wishes of an enjoyable and enthusiastic year of iGEM to every team and iGEM HQ.

Notebook

Show us how you spent your days.

Results/Conclusions

What did you achieve over the course of your semester?

Safety

What safety precautions did your team take? Did you take a safety training course? Were you supervised at all times in the lab?

Attributions

Who worked on what?

Human Practices

What impact does/will your project have on the public?

Fun!

What was your favorite team snack?? Have a picture of your team mascot?

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 After the analysis of the functions of the project last year, we discovered three main problems. The problems with their solutions are as follows:
 )There are safety issues with BactoCooler being released into the environment and specifically into the air.
- Through installing Kill-Switch mechanisms designed to activate on air contact, we can remove concerns of contamination.
+Through installing Kill-Switch mechanisms designed to activate on air contact, we can remove concerns of contamination.
 )BactoCooler synthesizes the urease enzyme which catalyzes the breakdown of the urea in the environment eventually leading to the accumulation of ammonia. Ammonia, being a basic compound, raises the pH of the environment after accumulating and causes alkalization. The highly basic environment results in the death of BactoCooler.
 To counteract the issue, a pH sensitive system which will detect the alkaline conditions will allow the secretion of an acidic buffer protein will be integrated in 'BactoCooler 2.0'.