http://2014hs.igem.org/wiki/index.php?title=Special:Contributions&feed=atom&limit=500&target=Peiwen+cai&year=&month=2014hs.igem.org - User contributions [en]2024-03-28T23:41:56ZFrom 2014hs.igem.orgMediaWiki 1.16.5http://2014hs.igem.org/File:Fun1.jpgFile:Fun1.jpg2014-06-20T17:31:16Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T17:28:18Z<p>Peiwen cai: /* Fun */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br><br />
[[File:kanshu.png|500px]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br><br />
[[File:Shenzhen_mengtu.png|600px]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br><br />
[[File:jianmomengtu.png|500px]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br><br />
[[File:anquan.png|500px]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/File:2333.jpgFile:2333.jpg2014-06-20T17:27:24Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T17:22:52Z<p>Peiwen cai: /* Fun */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br><br />
[[File:kanshu.png|500px]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br><br />
[[File:Shenzhen_mengtu.png|600px]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br><br />
[[File:jianmomengtu.png|500px]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br><br />
[[File:anquan.png|500px]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br><br />
[[File:2333.jpg|500px]]<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/File:23333.jpgFile:23333.jpg2014-06-20T17:22:06Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T17:20:09Z<p>Peiwen cai: /* Fun */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br><br />
[[File:kanshu.png|500px]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br><br />
[[File:Shenzhen_mengtu.png|600px]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br><br />
[[File:jianmomengtu.png|500px]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br><br />
[[File:anquan.png|500px]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br><br />
[[File:23333.jpg|500px]]<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T17:19:39Z<p>Peiwen cai: /* Fun */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br><br />
[[File:kanshu.png|500px]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br><br />
[[File:Shenzhen_mengtu.png|600px]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br><br />
[[File:jianmomengtu.png|500px]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br><br />
[[File:anquan.png|500px]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br><br />
[[File:23333.png|500px]]<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T17:18:09Z<p>Peiwen cai: /* Modelling */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br><br />
[[File:kanshu.png|500px]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br><br />
[[File:Shenzhen_mengtu.png|600px]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br><br />
[[File:jianmomengtu.png|500px]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br><br />
[[File:anquan.png|500px]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/File:Anquan.pngFile:Anquan.png2014-06-20T17:17:44Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T17:16:28Z<p>Peiwen cai: /* Safety */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br><br />
[[File:kanshu.png|500px]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br><br />
[[File:Shenzhen_mengtu.png|600px]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br><br />
[[File:jianmomengtu.png|600px]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br><br />
[[File:anquan.png|500px]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/File:Jianmomengtu.pngFile:Jianmomengtu.png2014-06-20T17:14:32Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T17:12:56Z<p>Peiwen cai: /* Modelling */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br><br />
[[File:kanshu.png|500px]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br><br />
[[File:Shenzhen_mengtu.png|600px]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br><br />
[[File:jianmomengtu.png|600px]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/File:Kanshu.pngFile:Kanshu.png2014-06-20T17:08:32Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T17:06:39Z<p>Peiwen cai: /* Project */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br><br />
[[File:kanshu.png|500px]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br><br />
[[File:Shenzhen_mengtu.png|600px]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T16:59:25Z<p>Peiwen cai: /* Lab */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br><br />
[[File:Shenzhen_mengtu.png|600px]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/File:Shenzhen_mengtu.pngFile:Shenzhen mengtu.png2014-06-20T16:58:51Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T16:55:31Z<p>Peiwen cai: </p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br><br />
[[File:Shenzhen_mengtu.png|100px]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMS/GalleryTeam:Shenzhen SZMS/Gallery2014-06-20T16:03:47Z<p>Peiwen cai: </p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
[[File:Shenzhen_SZMS_logo.png|right|150px|Shenzhen Middle School]]<br />
Here are some pictures of [[Team:Shenzhen SZMS]].<br />
<br />
== Photos ==<br />
{| align=center<br />
|align=center|[[File:9L7A3288.JPG|400px|Some of the team members are talking about the project. April 9, 2014]]<br />
|align=center|[[File:9L7A3647.JPG|400px|Zhang Yang is checking the cell culture.]]<br />
|-<br />
|align=center|[[File:SZMS 9L7A0141.JPG|400px|Some colorful bacteria]]<br />
|align=center|[[File:SZMS 9L7A0357.JPG|400px|Mr.Wang from Beijing Genomics Institute is teaching us. March 12, 2014]]<br />
|}<br />
{| align=center<br />
|align=center|[[File:helloworld1.jpeg|220px|E.coli the Fluorescent]]<br />
|align=center|[[File:helloworld2.jpeg|220px|E.coli the Fluorescent]]<br />
|align=center|[[File:helloworld3.jpeg|220px|E.coli the Fluorescent]]<br />
|align=center|[[File:helloworld4.jpeg|220px|E.coli the Fluorescent]]<br />
|}<br />
{| align=center<br />
|<br />
{|<br />
|align=center|[[File:SZMS (4).JPG|600px]]<br />
|-<br />
|align=center|[[File:SZMS (8).JPG|600px]]<br />
|}<br />
|<br />
{|<br />
|align=center|[[File:SZMS (1).JPG|200px]]<br />
|-<br />
|align=center|[[File:SZMS (2).JPG|200px]]<br />
|-<br />
|align=center|[[File:SZMS (3).JPG|200px]]<br />
|-<br />
|align=center|[[File:SZMS (9).JPG|200px]]<br />
|-<br />
|align=center|[[File:SZMS (6).JPG|200px]]<br />
|-<br />
|align=center|[[File:SZMS (7).JPG|200px]]<br />
|}<br />
|}<br />
=== Lectures in Secondary Schools ===<br />
''Learn more:[[Team:Shenzhen SZMS/Human Practice/Lectures]]''<br />
{| align=center<br />
|align=center|[[File:SZMS panel -1.png|300px]]<br />
|align=center|[[File:SZMS panel -2.png|300px]]<br />
|align=center|[[File:SZMS panel -3.png|300px]]<br />
|}<br />
{| align=right<br />
|align=center|[[File:Chuzhongbu1.jpg|400px]]<br />
|align=center|[[File:Chuzhongbu2.jpg|400px]]<br />
|}<br />
{| align=center<br />
|align=center|[[File:SZMS's lecture at junior high part of Shenzhen Middle School (1).jpg|200px]]<br />
|align=center rowspan=2|[[File:SZMS's lecture at junior high part of Shenzhen Middle School (8).jpg|400px]]<br />
|align=center|[[File:SZMS's lecture at junior high part of Shenzhen Middle School (2).jpg|200px]]<br />
|-<br />
|align=center|[[File:SZMS's lecture at junior high part of Shenzhen Middle School (3).jpg|200px]]<br />
|align=center|[[File:SZMS's lecture at junior high part of Shenzhen Middle School (4).jpg|200px]]<br />
|}<br />
{| align=left<br />
|align=center rowspan=2|[[File:SZMS's lecture at junior high part of Shenzhen Middle School (10).jpg|300px]]<br />
|align=center|[[File:SZMS's lecture at junior high part of Shenzhen Middle School (9).jpg|300px]]<br />
|align=center|[[File:SZMS's lecture at junior high part of Shenzhen Middle School (6).jpg|300px]]<br />
|-<br />
|align=center|[[File:SZMS's lecture at junior high part of Shenzhen Middle School (11).jpg|300px]]<br />
|align=center|[[File:SZMS's lecture at junior high part of Shenzhen Middle School (7).jpg|300px]]<br />
|}<br />
{| align=right<br />
|align=center|[[File:Lianhua1.jpg|300px]]<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (5).jpg|300px]]<br />
|align=center|[[File:Lianhua2.jpg|300px]]<br />
|}<br />
{| align=center<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (8).jpg|200px]]<br />
|align=center rowspan=2|[[File:SZMS's lecture at Lianhua Middle School (6).jpg|400px]]<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (7).jpg|200px]]<br />
|-<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (11).jpg|200px]]<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (10).jpg|200px]]<br />
|-<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (12).jpg|200px]]<br />
|align=center rowspan=2|[[File:SZMS's lecture at Lianhua Middle School (4).jpg|400px]]<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (14).jpg|200px]]<br />
|-<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (13).jpg|200px]]<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (21).jpg|200px]]<br />
|}<br />
{|<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (18).jpg|300px]]<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (19).jpg|300px]]<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (20).jpg|300px]]<br />
|}<br />
{| align=right<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (1).jpg|300px]]<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (2).jpg|300px]]<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (3).jpg|300px]]<br />
|}<br />
<br style="clear:both;"><br />
<br />
=== Laboratory Open Day ===<br />
''Learn more:[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]''<br />
{| align=center<br />
|align=center|[[File:SZMS_Laboratory_Open_Day_poster_-1.png|400px]]<br />
|align=center|[[File:SZMS_Laboratory_Open_Day_poster_-2.png|400px]]<br />
|}<br />
{| align=center<br />
|align=center|[[File:Kaifangri1.jpg|300px|Laboratory Open Day]]<br />
|align=center|[[File:Kaifangri2.jpg|300px|Laboratory Open Day]]<br />
|align=center|[[File:Kaifangri3.jpg|300px|Laboratory Open Day]]<br />
|-<br />
|align=center|[[File:Kaifangri4.jpg|300px|Laboratory Open Day]]<br />
|align=center|[[File:Kaifangri5.jpg|300px|Laboratory Open Day]]<br />
|align=center|[[File:Kaifangri6.jpg|300px|Laboratory Open Day]]<br />
|}<br />
{| align=center<br />
|<br />
{|<br />
|align=center|[[File:SZMS (21).JPG|200px]]<br />
|-<br />
|align=center|[[File:SZMS (15).JPG|200px]]<br />
|-<br />
|align=center|[[File:SZMS (17).JPG|200px]]<br />
|-<br />
|align=center|[[File:SZMS (18).JPG|200px]]<br />
|-<br />
|align=center|[[File:SZMS (19).JPG|200px]]<br />
|-<br />
|align=center|[[File:SZMS (20).JPG|200px]]<br />
|}<br />
|<br />
{|<br />
|align=center|[[File:SZMS (14).JPG|600px]]<br />
|-<br />
|align=center|[[File:SZMS (16).JPG|600px]]<br />
|}<br />
|}<br />
<br style="clear:both;"><br />
=== World DNA and Genome Day ===<br />
''Learn more:[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]''<br />
{| align=center<br />
|align=center|[[File:SZMS poster for BIT's 5th World DNA and Genome Day (1).JPG|400px|An English poster for World DNA and Genome Day]]<br />
|align=center|[[File:SZMS poster for BIT's 5th World DNA and Genome Day (2).JPG|400px|An Chinese poster for World DNA and Genome Day]]<br />
|}<br />
{| align=center<br />
|align=center|[[File:Dalian1.jpg|300px|BIT's 5th World DNA and Genome Day]]<br />
|align=center|[[File:Dalian2.jpg|300px|BIT's 5th World DNA and Genome Day]]<br />
|align=center|[[File:Dalian3.jpg|300px|BIT's 5th World DNA and Genome Day]]<br />
|-<br />
|align=center|[[File:SZMS with Ada Yonath.JPG|300px|SZMS with Ada Yonath]]<br />
|align=center|[[File:SZMS with Akira Suzuki.JPG|300px|SZMS with Akira Suzuki]]<br />
|align=center|[[File:SZMS with Dan Shechtman.JPG|300px|SZMS with Dan Shechtman]]<br />
|}<br />
{|<br />
|align=center|[[File:SZMS with George F. Smoot.JPG|400px|SZMS with George F. Smoot]]<br />
|align=center|[[File:SZMS with Hartmut Michel.JPG|400px|SZMS with Hartmut Michel]]<br />
|}<br />
{| align=right<br />
|align=center|[[File:SZMS with Luc Montagnier.JPG|400px|SZMS with Luc Montagnier.JPG]]<br />
|align=center|[[File:SZMS with Vladimir Khavinson.JPG|400px|SZMS with Luc Montagnier.JPG]]<br />
|}<br />
<br style="clear:both;"><br />
<br />
== Our cuties ==<br />
{| align=right<br />
|align=center|[[File:SZMS brochure 3.jpg|150px]]<br />
|align=center|[[File:Shenzhongigem2014.jpg|400px]]<br />
|align=center|[[File:Albumface.jpg|400px]]<br />
|}<br />
{| align=center<br />
|align=center|[[File:Huoxing1.jpg|220px|E.coli on Mars]]<br />
|align=center|[[File:Huoxing3.jpg|220px|E.coli on Mars]]<br />
|align=center|[[File:Huoxing4.jpg|220px|E.coli on Mars]]<br />
|align=center|[[File:Huoxing2.jpg|220px|E.coli on Mars]]<br />
|}<br />
{| align=center<br />
|align=center|[[File:SZMS brochure 2.jpg|175px]]<br />
|align=center|[[File:SZMS brochure 4.jpg|175px]]<br />
|align=center|[[File:SZMS brochure 5.jpg|175px]]<br />
|align=center|[[File:SZMS brochure 6.jpg|175px]]<br />
|align=center|[[File:SZMS brochure 7.jpg|175px]]<br />
|}</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMS/iGem_Safety_Q%26ATeam:Shenzhen SZMS/iGem Safety Q&A2014-06-20T16:03:34Z<p>Peiwen cai: </p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
Here are our answers for [https://2014hs.igem.org/Safety iGEM Safety Questions].<br />
<br />
==Q&A==<br />
===Question 1===<br />
1.Would any of your project ideas raise safety issues in terms of:<br />
*researcher safety,<br />
*public safety, or<br />
*environmental safety'''<br />
----<br />
Organisms:<br />
<br />
:E.coli<br />
:#Risk Group 1 (no or low individual and community risk) - WHO Laboratory Biosafety Manual<br />
:#BSL 1 - The list of human to human transmission of pathogenic microorganisms<br />
<br />
:Scindapsus aureus<br />
:#Risk Group 1 (no or low individual and community risk) - WHO Laboratory Biosafety Manual<br />
<br />
Risks:<br />
#Potential pathogenicity to team members since e.coli is infectant and would cause disease when accumulating.<br />
#Potential risk of the release of modified gene out of the lab and possible pollution of other organisms.<br />
<br />
Addressing issues:<br />
#Suicide system<br />
#Lab Safety Rules: Good microbiological techniques(GMT)<br>General requirements for laboratory biosafety GB-19489 2008<br />
<br />
Safety training:<br />
#WHO Laboratory Biosafety Manual<br />
#General requirements for laboratory biosafety GB-19489 2008<br />
#Biosafety in the Microbiological and Biomedical Laboratories Manual,3th edition CDC/NIH <br />
<br />
Accident out of plan:<br />
#Release of modified gene and possible genetic pollution if the containment procedures go wrong.<br />
#Release of bacteria and possible health problem of team members.<br />
<br />
Addressing issues:<br />
#Suicide system to prevent genetic pollution.<br />
#Protection suits for researchers.<br />
#Medical examination in hospital.<br />
#Complete sterilization in the lab.<br />
<br />
=== Question 2 ===<br />
2.Do any of the new BioBrick parts (or devices) that you made this year raise safety issues? If yes,<br />
*Did you document these issues in the Registry?<br />
*How did you manage to handle the safety issue?<br />
*How could other teams learn from your experience?<br />
----<br />
All the biosafety levels of the parts in our project are BSL 1.<br />
<br />
Suicide system<br />
<br />
=== Question 3 ===<br />
3.Is there a local biosafety group, committee, or review board at your institution?<br />
*If yes, what does your local biosafety group think about your project?<br />
*If no, which specific biosafety rules or guidelines do you have to consider in your country?<br />
----<br />
No.<br />
<br />
WHO Laboratory Biosafety Manual<br />
<br />
General requirements for laboratory biosafety GB-19489 2008<br />
<br />
Biosafety in the Microbiological and Biomedical Laboratories Manual,3th edition CDC/NIH<br />
<br />
<br />
=== Question 4 ===<br />
4.Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?<br />
----<br />
#Safety level examination of parts and devices in lab.<br />
#Clear biosafety hierarchy system of BioBrick parts(or devices) and publish the classification on the website of iGem for participants to consult.<br />
#Suicide system to prevent genetic pollution when release of organism happens.<br />
<br />
==Reference==<br />
#''The list of human to human transmission of pathogenic microorganisms'';<br />
#''General requirements for laboratory biosafety GB-19489 2008''.</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMS/LabTeam:Shenzhen SZMS/Lab2014-06-20T16:03:22Z<p>Peiwen cai: </p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
Here you can find out how we build our plasmid. The plasmid map and information of parts used is given in [[Team:Shenzhen SZMS/Project]].<br />
<br />
==Schedule==<br />
{| class="wikitable"<br />
|-<br />
!width=50px|No. !!width=300px| Procedure !!width=100px|Backbone !!width=250px| Status of the Plasmid<br />
|-<br />
|1<br />
|[K115016] Spe1,Pst1 Double Digestion, Electrophoresis detection,DNA refinement;[C0051] X ba1,Pst1Double Digestion, Gel extraction;Combination<br />
|1C3(K115016)<br />
|[K115016+C0051]<br />
|-<br />
|2<br />
|[J23106] Spe1,Pst1 Double Digestion, Electrophoresis detection,DNA refinement;[K115016+C0051] X ba1,Pst1Double Digestion, Gel extraction;Combination<br />
|1A2(J23106)<br />
|J23106+[K115016+C0051]<br />
|-<br />
|3<br />
|[J2s3106+ K115016+C0051] EcoR1,Spe1Double Digestion, Electrophoresis detection,DNA refinement;[B0051] EcoR1,Xba1 Electrophoresis detection,DNA refinement;Combination<br />
|1C3(B0015)<br />
|[J23106+K115016+C0051]+B0015<br />
|-<br />
|4<br />
|[B0030] Spe1,Pst1 Double Digestion, Electrophoresis detection, DNA refinement;[EC:1.7.7.2] Xba1, Pst1Double Digestion, Gel extraction;Combination<br />
|1A2(B0030)<br />
|B0030+EC:1.7.7.2<br />
|-<br />
|5<br />
|[R0051] Spe1, Pst1Double Digestion, Electrophoresis detection,DNA refinement;[B0030+ EC:1.7.7.2] Xba1,Pst1 Double Digestion, Gel extraction;Combination<br />
|1A2(R0051)<br />
|R0051+[B0030+ EC:1.7.7.2]<br />
|-<br />
|6<br />
|[B0030] Spe1, Pst1Double Digestion, Electrophoresis detection,DNA refinement;[K145015] Xba1,Pst1Double Digestion, Gel extraction; Combination<br />
|1A2(B0030)<br />
|B0030+K145015<br />
|-<br />
|7<br />
|[B0030+ K145015] EcoR1, Spe1Double Digestion, Electrophoresis detection,DNA refinement;[B0015] EcoR1, Xba1Double Digestion,Electrophoresis detection,DNA refinement;Combination<br />
|1C3(B0015)<br />
|[B0030+ K145015]+B0015<br />
|-<br />
|8<br />
|[R0051+B0030+ EC:1.7.7.2] Spe1, Pst1Double Digestion,Gel extraction;[B0030+ K145015+B0015]Xba1, Pst1 Gel extraction;Combination<br />
|1C3(B0030+K145015+B0015)<br />
|[R0051+B0030+ EC:1.7.7.2]+[B0030+K145015+B0015]<br />
|-<br />
|9<br />
|[K115017] Spe1, Pst1Double Digestion, Electrophoresis detection,DNA refinement;[C0051] Xba1, Pst1Double Digestion,Gel extraction; Combination<br />
|1C3(K115016)<br />
|[K115017+C0051]<br />
|-<br />
|10<br />
|[J23106] Spe1,Pst1Double Digestion, Electrophoresis detection,DNA refinement;[K115017+C0051] Xba1, Pst1Double Digestion, Gel extraction;Combination<br />
|1A2(J23106)<br />
|J23106+[K115017+C0051]<br />
|-<br />
|11<br />
|[J23106+ K115017+C0051] EcoR1, Spe1Double Digestion,Gel extraction;[B0015]EcoR, Xba1Double Digestion, Electrophoresis detection,DNA refinement,Combination<br />
|1C3(B0015)<br />
|[J23106+ K115017+C0051]+B0015<br />
|-<br />
|12<br />
|[The first part] Spe1,Pst1 Double Digestion;[The second part] Xba1,PstL Double Digestion;Gel extraction; Combination<br />
|1C3(The first part)<br />
|1st+2nd<br />
|-<br />
|13<br />
|[The first and second parts] Spe1、Pst1Double Digestion; [The third part] Xba1、Pst1Double Digestion;Gel extraction; Combination<br />
|1C3(The third part)<br />
|1st +2nd+3rd<br>Done<br />
|}<br />
<br />
==Log Book==<br />
{| class="wikitable"<br />
|-<br />
!width=50px| Date !!width=300px| Event !!width=300px| Results !!width=200px| Problem summary<br />
|-<br />
|Apr.3<br />
|B0015(chl) B0030(chl) Plasmid Transformation<br />
|Done <span style="color:grey">(yet this B0030 is single error)</span><br />
|<br />
|-<br />
|Apr.4<br />
|Single clone selected from the work on Apr.3 <span style="color:red">(In order to be pithy, this process is omitted from the table in all the following daily work)</span><br />
|Done<br />
|<br />
|-<br />
|Apr.5<br />
|Plasmid preped and culture restored from the bacterial suspension on Apr.4 <span style="color:red">(omitted later for pithiness and convenience as well)</span><br />
|Done<br />
|<br />
|-<br />
|Apr.6<br />
|K115016(CHL) K115017(CHL) C0051(CHL) transformation<br />
|Done<br />
|<br />
|-<br />
|May.2<br />
|J23106(AMP) K082003(CHL,as GFP+LVA) transformation (help offered by Mentor Wang)<br />
|Done<br />
|<br />
|-<br />
|May.5<br />
|Digestion: K115016(S/P) C0051(X/P) C0051(E/S) B0015(E/X)<br>Electrophoresis<br>Gel extraction<br>16℃ overnight<br />
|Done<br />
|<br />
|-<br />
|May.6<br />
|Product from 5.5(CHL)transformed<br />
|Failed<br />
|Might be the problem during the plasmid prep<br />
|-<br />
|May.7<br />
|Plasmid mentioned above repreped<br />
|Done<br />
|<br />
|-<br />
|May.13<br />
|Digestion: C0051(E/S) B0015(S/P) K082003(E/S)<br>Electrophoresis<br>Gel extraction<br>16℃ overnight<br />
|Done, C0051-B0015<br>K082003-B0015 gained<br />
|<br />
|-<br />
|May.14<br />
|Products from 5.13(CHL)all transformed<br />
|Done, C0051-B0015<br>K082003-B0015 gained<br />
|<br />
|-<br />
|May.15<br />
|Gel confirmation (products from 5.14), comparison with gel of B0015<br />
|Done, product longer than the original plasmid<br />
|<br />
|-<br />
|May.27<br />
|Digestion: C0051-B0015(X/P) K115016(S/P) K115017(S/P)<br>Electrophoresis<br>Gel extraction<br>Combination<br>Transformation(CHL)<br />
|Done,<br>K115016-C0051-B0015<br>K115017-C0051-B0015<br>gained.<br />
|<br />
|-<br />
|May.28<br />
|B0030(AMP) transformation<br />
|Done; informed that R0051 requires PCR in order to get the primer of EC 1.7.7.2<br />
|<br />
|-<br />
|May.29<br />
|Digestion: J23106(S/P)<br>K115016-C0051-B0015(X/P)<br>K115017-C0051-B0015(X/P)<br>Electrophoresis<br>Gel extraction<br>Combination<br>Transformation(AMP)<br />
|Done,<br>J23106-K115016-C0051-B0015(27℃CI-protein)<br>J23106-K115017-C0051-B0015(32℃CI-protein)<br>gained.<br />
|<br />
|-<br />
|May.30<br />
|The result of sequencing of C0051-B0015<br>K082003-B0015 received<br />
|Correctly combined<br />
|<br />
|-<br />
|Jun.2<br />
|Digestion: B0030(S/P) K082003-B0015(X/P)<br>Electrophoresis<br>Gel extraction<br>Combination<br>Transformation(AMP)<br />
|Done<br />
|<br />
|-<br />
|Jun.8<br />
|The results of sequencing of J23106-K115016-C0051-B0015(27℃CI) and J23106-K115017-C0051-B0015(32℃ CI)received.<br />
|Correctly combined<br />
|<br />
|-<br />
|Jun.9<br />
|Digestion:B0030-K082003-B0015(E/S)<br>J23106-K115017-C0051-B0015(E/X)<br>Electrophoresis<br>Gel extraction<br>Combination<br>Transformation(AMP)<br />
|Done with transformation;<br>Yet failed in the electrophoretic analysis.<br />
|Disconnected<br />
|-<br />
|Jun.10<br />
|<br />
|ciF1 ciF2 ciF3: primers to synthesize EC 1.7.7.2 and PEP (additional PEP R0051-B0030-) gained.<br />
|<br />
|-<br />
|Jun.11<br />
|With the original blue 2X Master Mix PCR round 1,2,3 implemented<br />
|Failed; only primer founded in the electrophoretic analysis<br />
|<br />
|-<br />
|Jun.12<br />
|Round 1 from 6.11 repeated in the morning;<br>Round 3 PCR with 2X hi-fi Master Mix at noon<br />
|Failed in the morning but done in the afternoon;<br>R0051-B0030-1.7.7.2 recycled product gained.<br />
|might be the problem of MIX<br />
|-<br />
|Jun.13<br />
|Digestion: 27℃ CI(S/P)R0051-B0030-1.7.7.2(X/P)<br>32℃ CI(E/X) <br>B0030-K082003-B0015(E/S)<br>Electrophoresis<br>Gel<br>extraction<br>Combination<br>Transformation.<br />
|Failed, no culture founded on the dish.<br />
|Unknown error; might be the problem of extracted gel.<br />
|-<br />
|Jun.15 <br />
|Digestion: 27℃ CI(S/P)R0051-B0030-1.7.7.2(X/P)<br>32℃ CI(E/X) <br>B0030-K082003-B0015(E/S)<br>Electrophoresis<br>Gel<br>extraction<br>Combination<br>Transformation.<br />
|According to the result of electrophoresis, the length of the digested and combined part<br> is the same as that of the primitive plasmid;<br> yet we cannot exclude the problem of plasmid prep; might have failed. <br />
|A tube was missed during the digestion yet was made up later; do not know about its possible effect yet.<br />
|}</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMS/ModellingTeam:Shenzhen SZMS/Modelling2014-06-20T16:03:08Z<p>Peiwen cai: </p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
In order to assess the efficiency of our E.coli babysitter, we have made math models for the core system in it, EC 1.7.7.2, which functions as to heat up the environment to provide a more suitable temperature for the plant.<br />
<br />
EC 1.7.7.2 is basically a ferredoxin-nitrate reductase; with the required substrates nitrate and reduced ferredoxin, EC 1.7.7.2 is able to catalyze the reactions below:<br />
<br />
nitrite + H<sub>2</sub>O + oxidized ferredoxin ⇄ nitrate + reduced ferredoxin + 2H<sup>+</sup><br />
<br />
HNO<sub>2</sub> + NH<sub>3</sub> = N<sub>2</sub>↑+ 2H<sub>2</sub>O<br />
<br />
obviously as suggested above,the first reaction is a reversible one; the heat we need is actually from the second reaction since the first one is basically to convert nitrate provided by our special LB into nitrite.On the other hand, the warmth released by the first reaction is so little that it has nearly no effect on the result. Consequently,we make only the math models for the second reaction, whose substrate is nitrate. As calculated, the general warmth that would be released is 334.55 KJ/mol, which is a theoretical datum.<br />
<br />
However,considering the fact that we do not have adequate data to carry out our math models, we finally decide to use proportionality instead of accurate data. Both of the following charts are established by Excel.<br />
<br />
The first one is a model under the condition that purely only EC 1.7.7.2 is functioning, which means, the concentration of it remains unchanged: (grey line → temperature inside the systerm)<br />
<br />
[[File:nongdumeiyoubianlatest.png|800px]]<br />
<br />
The second one is a model under the condition that RNA Thermometer in the first part of our entire plasmid is functioning to start the heating-up system which is mostly EC 1.7.7.2 in certain extent of external temperature (<27℃). Since it is being manipulated by the other system, the concentration of EC 1.7.7.2 can no long remain unchanged, but changed over time: (black line → temperature inside the systerm)<br />
<br />
[[File:nongdubianleahahaha.png|800px]]<br />
<br />
General information about EC 1.7.7.2 can be found in the following link: http://www.genome.jp/dbget-bin/www_bget?ec:1.7.7.2.</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMS/ProjectTeam:Shenzhen SZMS/Project2014-06-20T16:02:28Z<p>Peiwen cai: </p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
Find more about our project below!<br />
<br />
== Background ==<br />
'''E. coli Plant-sitter'''<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation. We hope that our toolkit will aid the process of plant cultivation, enlarge the public understanding of synthetic biology, and make the methods and technology of synthetic biology accessible to people’s daily life.<br />
Daily life – yes, on earth our Plant-sitter can be well applied to the growth of plants – but what about…<br />
Wait for it –<br><br />
'''MARS!'''<br><br />
[http://en.wikipedia.org/wiki/Mars Mars], lying four hundred million kilometers from earth, has the most earth-like seasons due to Mars and Earth’s similar tilts of rotational axes. As a result, in order for men to make use of Martian resources and to develop its potential as an alternative human residence in the universe, a Mars Migration Program was issued SpaceX, indicating that founding a human community on Mars requires 1) releasing carbon dioxide to make the atmosphere denser and to create raw materials for photosynthesis; '''2) cultivating plants beside human residence to generate oxygen.'''<br><br />
'''– which is where our E.coli Plant-sitter helps.'''<br />
Although there will be basic heat supply in the houses, the healthy growth of the plants is still under threat as Mars’ surface temperature varies from -143℃ to 35℃. Therefore, we came up with a system that protects the plants from overly cold environment through smart temperature control, keeping the temperature at around 27℃, aiding the plants who are burdened with the glorious purpose of generating oxygen to defend against the Jotunheim weather on Mars.<br />
<br />
<br />
Plants whose preferential temperature ranges from 25~30℃ are exposed to the threat of low productivity and unhealthy growth in overly cold environments. Therefore, we came up with a system that aids the healthy growth of these plants by protecting them from overly low temperatures through smart temperature control. Our system of temperature moderation includes two parts, temperature sensing and temperature regulating.<br />
<br />
[[File:huoxing1.jpg|200px]]<br />
[[File:huoxing3.jpg|200px]]<br />
[[File:huoxing4.jpg|200px]]<br />
[[File:huoxing2.jpg|200px]]<br />
<br />
== Plasmid and Working Details ==<br />
{| class="wikitable" align=right width=400px |+ Parts we used<br />
|-<br />
|colspan=3 align="center"|[[File:Plant-sitter temperature control Map latest version.jpg|400px|The plasmid map]]<br />
|-<br />
!width=10%| Compile !!width=30%| Function !!width=60%| Introduction <br />
|-<br />
| J23106 || Promoter || It promotes the transcription of the following parts. [http://parts.igem.org/Part:BBa_J23106 More]<br />
|-<br />
| R0051 || CI regulated Promoter || It promotes the transcription without CI. When CI is binding to it,it is repressed. [http://parts.igem.org/Part:BBa_R0051 More]<br />
|-<br />
| K115016 || 27℃ RBS || It only promotes the translation when the temperature is over 27℃ [http://parts.igem.org/Part:BBa_K115016 More]<br />
|-<br />
| K115017 || 32℃ RBS || It only promotes the translation when the temperature is over 32℃ [http://parts.igem.org/Part:BBa_K115017 More]<br />
|-<br />
| B0030 || strong RBS || It can promotes the translation strongly. [http://parts.igem.org/Part:BBa_B0030 More]<br />
|-<br />
| C0051 || Coding sequence || It is the coding sequence of CI Protein. [http://parts.igem.org/Part:BBa_C0051 More]<br />
|-<br />
| 1.7.7.2 (KEGG) || Coding sequence || We got this part from bacillus subtilis best7613 strain.It catalyzes the reaction which converts Nitrate to Nitrite. [http://www.genome.jp/dbget-bin/www_bget?ec:1.7.7.2 More]<br />
|-<br />
| K145015 || Coding scquence || It is the coding sequence of GFP. [http://parts.igem.org/Part:BBa_K145015 More]<br />
|-<br />
| B0015 || Terminator || It can stop the transcription. [http://parts.igem.org/Part:BBa_B0015 More]<br />
|}<br />
<br />
The process of temperature sensing involves a promoter, a 27℃ RNA thermometer, and cI. The process of temperature regulating involves a cI regulated promoter, an enzyme which catalyzes an exothermic chemical reaction, a 32℃ RNA thermometer, and a GFP LVA device which serves to indicate the occurrence of a change in temperature. The promoter in temperature sensing part is consistently on and thus so is the process of cI’s transcription from DNA to RNA. When the temperature is below 27℃, the 27℃RNA thermometer takes a curved, locked structure which does not enable translation from RNA to protein cI, which in turn allows the activation of the cI regulated promoter, the production of the enzyme, the occurrence of an exothermic reaction which heats up the environment, and the expression of GFP LVA tag which gives out green light to indicate that the temperature is below 27℃ and that our device of temperature control is doing its work. When the temperature rises and reaches 27 ℃ as a result of the exothermic reaction, the 27℃ RNA thermometer enables the translation of cI protein, which in turn represses the cI regulated promoter, ending the release of heat through the exothermic reaction by stopping the production of the enzyme which catalyzes that reaction. As a result of an inactive promoter (the cI regulated), the GFP also stops its expression and is decomposes, indicating that the temperature has risen from below 27 ℃ to 27 ℃and that our system of temperature control has done its work.<br />
If the 27℃ RNA thermometer fails to stop the heating process at around 27℃ and the temperature keeps rising up to 32℃, the 32℃ RNA thermometer is activated, leading to the presence of protein cI, which places a double-check on the inhibition of the heating process.<br />
<br />
=== Temperature Sensing ===<br />
This process involves BBa_J23106 (promoter), BBa_K115016 (27℃ RNA thermometer), and cI (Repressor protein cI).<br />
BBa_J23106 is consistently on and thus so is the process of cI’s transcription from DNA to RNA. When the temperature is below 27℃, <br />
the 27℃ sensitive RNA thermometer does not enable translation from RNA to protein cI, which in turn allows the activation of BBa_R0051, the production of the enzyme, the occurrence of an exothermic reaction which heats up the environment, <br />
and the expression of GFP LVA tag which gives out green light to indicate that the temperature is below 27℃ and that our device of temperature control is doing its work.<br />
<br />
When the temperature rises and reaches 27 ℃ as a result of the exothermic reaction, BBa_K115016 (27℃ RNA thermometer) enables the translation of cI protein, which in turn represses BBa_R0051, <br />
ending the release of heat through the exothermic reaction by stopping the production of the enzyme which catalyzes that reaction.<br />
<br />
As a result of an inactive promoter (BBa_R0051), the GFP also stops its expression and is decomposes,<br />
indicating that the temperature has risen from below 27 ℃ to 27 ℃and that our system of temperature control has done its work.<br />
<br />
If BBa_K115016 (27℃ RNA thermometer) fails to stop the heating process at around 27℃ and the temperature keeps rising up to 32℃, BBa_K115017 (32℃ RNA thermometer) is activated, leading to the presence of protein cI, <br />
which places a double-check on the inhibition of the heating process.<br />
<br />
The process by which our temperature-control system operates may be better illustrated in the following flow chart:<br />
<br />
=== Temperature Regulating ===<br />
The process of temperature regulating involves BBa_J45503 (cI regulated promoter), an enzyme which catalyzes an exothermic chemical reaction, BBa_K115017 (32℃ RNA thermometer) and a GFP LVA device which serves to indicate the occurrence of a change in temperature.<br />
<br style="clear:both;"><br />
----<br />
The following is a flowchart explaining how our E. coli Plant-sitter works.<br />
<br />
[[File:IGem_-_New_Page_Correcto.png|center|900px]]</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMS/Team_MembersTeam:Shenzhen SZMS/Team Members2014-06-20T16:02:12Z<p>Peiwen cai: </p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
There are 17 students and 3 instructors in our team. Their personal information is given here:<br />
<br />
{| class="wikitable"<br />
|-<br />
!width=100px| Name !!width=300px| Photo !!width=300px| Self-intro !!width=200px| Work <br />
|-<br />
| Qian Yuhan<br>钱雨涵 || [[File:Qian Yuhan.JPG|300px]] || Yuhan Qian, a senior 2 student, is a member of the Experiment Group who has learnt about Molecular Biology and Bioinformatics herself. According to what she said, although staying in the laboratory with the equipment was delightful, learning the theories was also essential for speculating the possible results, analyzing the phenomena and finding the factors that can lead to failures. || team leader, experiment group member<br />
|-<br />
| Wang You<br>王悠 || [[File: Wang You meizhaopian.JPG|300px]] || You Wang,now a senior 2 student, has learned the expansive possibility of synthetic biology to faciliate human life since senior 1.Amazed and enchanted by the magic of it, she attends iGem with great passion, exploring more surprise and potential in this field.In SZMS iGem Team, she exercises herself by contacting head quaters, searching for parts, designing plasmid and doing experiments.To build communication with other iGem Teams, she also attend the iGem meeting in Hongkong. "iGem is not only a competition", smilingly comments she,"It's a impressively memorable lesson." || experiment, human practice, theory<br />
|-<br />
| Lin Jingfei<br>林静菲 || [[File:Lin Jingfei at SZMS.jpg|300px]] || Lin Jingfei, a senior one student in Shenzhen Middle School, finds her great interest in synthetic biology. After participating the Human Practice Group, she helped make the advertisement pamphlet and posters. She also helped the group leader, Jing, instruct the visitors on the Open Day of the Laboratory. Believing that the art and science can be combined together, she is trying to contribute more and more for the 2014 SZMS Team. || human practice<br />
|-<br />
| Pan Sixuan<br>潘思璇 || [[File:Pan Sixuan at SZMS.jpg|300px]] || Sixuan is a sophomore student and a member of the Theory group for SZMS iGEM Team. Motivated by an interest of chemistry and molecular biology, she helped plan and visualize the plasmid by using Snapgene. Almost drawn herself in published papers and DNA sequences, she fortunately did not get blind but managed to build the model that fits our objective. With good skills for communication, she went to Dalian for the BIT’s 5th Genome Day and promoted our project there by presenting our posters. “By gradually accessing to the field of genetic research, I perceived the possibility of making miracles through genome studies,” She said. || Theory group member, responsible for researching the theoretical construction of our project, plasmid planning and visualization, promoting our project on BIT’s 5th Genome Day and project presentation<br />
|-<br />
| Cai Peiwen<br>蔡佩汶 ||[[File:PeiwenCaiProfilePic.JPG|300px]] || Peiwen Cai, a disguised senior 2, has delayed her high school life for one more year because of her exchange year in Germany, where she had totally set her mind free and lived wildly. Under such an circumstance, she discovered that her enthusiasm was never for advertising or political campaign but for biomed. This lifechanging discovery has motivated her to join SZMS iGem team and led her ever since then into the track of biomed. Challenge never tears her down but builds up her sense of humor.To crack down the human neuro-dilemmas with medicine is one of her two lifelong goals, the other one of which is to be a lobbyist.|| Former theory group member, mainly in math model group and wiki group.Tech girl working for the team. <br />
|-<br />
| Zheng Bingmiao<br>郑冰苗 || [[File:Zheng Bingmiao at SZMS.JPG|300px]] || Bingmiao Zheng is a senior one student from Shenzhen Middle School. Bingmiao is interested in biology and design, so she chooses to be a member of Human Practice of our project, Plant-Sitter, for the 2014th iGEM competition. || She helped Jing make the hand-made poster for BIT's Genome Day and do some publicity, like the Open Day of the laboratory.<br />
|-<br />
| Zhang Yang<br>张杨 || [[File:Zhang Yang at SZMS.JPG|300px]] || This man, who is a senior two student, attended the BIT’s 5th Genome Day to introduce our team to those people who attended this international meeting. Some professors from America and China show high interest in our project. Zhang Yang likes experiments a lot. It just like that he is always in the laboratory. He does learn a lot through this competition. || He takes part in almost all parts of the team except the building of the model. <br />
|-<br />
| Jing Yufei<br>景羽飞 || [[File:Jing Yufei.jpg|300px]] || Yufei Jing is a senior two student from Experimental Curriculum, Shenzhen Middle School. She love biology because it is a subject with many unknowns, that makes her full of curiosity. || She is the leader of the Human Practice Group, arrange the activities of this group and also draw many illustrations for our project. <br />
|-<br />
| Zhou Yineng<br>周益能 || [[File:Zhou Yineng at SZMS.JPG|300px]] || Zhou Yineng is a senior one student from Standard Curriculum, Shenzhen Middle School. As a biology lover, he spent a lot of time in the laboratory, which helps him gain a lot of experience of experiment, and as a result, he has become one of team members who have done a complete cycle including digestion, electrophoresis, gel extraction, combination and transformation, although he even didn't know what transcription means at first. || human practice, experiment, wiki.<br />
|-<br />
| Feng Yanming<br>冯彦铭 || [[File:Feng Yanming.jpg|300px]] || Feng Yanming(Danny Feng) , a senior-two student from Shenzhen Hgih School of Science(HSS). He is the Vice-President of Students’ Union and the President of IT Club in the School. He is keen on computer science, web designing, programming and viedo making(Included Viedo clipping&making special effect by using AE.) “A lof of my classmates asked me why I’m good at computer, I just told them, the curiosity is the best teacher of learning.” He said, "It's a whole new chance&chanllenge for me to attend the iGem competition. I love biology, but I don't know much about the experiment of molecular biology before. " When Danny came to the lab for the first time, he even didn't know how to use the pipettor. But he worked hard, and made a big progress."It's really a valuable experience for me. I've studied a lot during the experiment, and I really enjoyed the time I stayed with my team members, they are very friendly to me." || Web designing,video making,human practice<br />
|-<br />
| Yan Ming<br>闫铭 || [[File:Yan Ming at SZMS.jpg|300px]] || Ming Yan, one of the senior one students in SMS IGEM team, finds her interest in biology after going through the whole process of the IGEM competition. She has met many excellent people (including her teachers, teammates, and many students who are also interested in synthetic biology) because of participating the competition. She enjoys the competition every much. || experiment<br />
|-<br />
| Yang Hongkang<br>杨泓康 || [[File:Yang Hongkang.png|300px]] || Yang Hongkang is a senior one students and a member of SZMS iGEM Team’s theory group. Having an interest in biochemistry and mathematics, and a career expectation in mathematical and theoretical biology, he participated in iGEM competition as to take a step toward his goal. With the passion for his work, he designed the key reactions of the exothermic system, established the early program of the enzyme engineering, and participated in the math-modelling of the project. || Theory group member, responsible for the constructing of the chemical reactions and mathematical model.<br />
|-<br />
| Chen Qingyi<br>陈清怡 ||[[File:Chen Qingyi.png|300px]]|| Qingyi Chen, a senior 3 student in Shenzhen middle school, has strong propensity toward biology and statistics which are the fundamental consists of the society. She is very ambitious and motivate to the tasks. Having virtue of tenacity and inquisitive, She is always curious about the truth and dedicates in solving problem. For her, biology is very amazing world which construct creatures. The complex structure of protein and the beauty of plants always draw her passion. Hoping to discovery further about biology, she always stays hungry to learn more about biology.|| Theoretical member. She helps the team to find out innovative ideas and research the feasibility of specific ideas.<br />
|-<br />
| Wang Zhaobo<br>王照博 || [[File:Wang Zhaobo.JPG|300px]] || Wang Zhaobo is a senior 1 student in Shenzhen Middle School. He is an important member of Human Practice who is interested in Biology. Also he is a member of Shenzhen Photographs Alliance and he becomes an awesome cameraman in our iGEM. “Delighting you always” he always || photograph<br />
|-<br />
| Yang Yuwei<br>杨雨薇 || [[File:Yang Yuwei meizhaopian.JPG|300px]] || Yuwei is a senior two student who is dedicated into bio-medical engineering and psychology from the International Curriculum from Shenzhen Middle School. As a member of human practice group for the 2014th SZMS iGem Team, she believes that the creativity and imagination which are mostly demonstrated in art work can also be applied into the fantastic micro cellular world. For improving human's living condition and deepening the research in diseases or creatures, she steadfastly intends to go far in bio-med and psychology fields. " People are DNA's way of making more DNA", she believes that one day the secrets of nature will be exposed and hopefully she will become one of those "precursors". || Human practice group member, responsible for propagandizing the project and publicity such as opening lectures with teammates in other schools. She also takes charge of organizing the team trip plan during the competition days and contacting with the committee for the invitation letters, etc. <br />
|-<br />
| Xiao Linfan<br>肖琳凡 || [[File:Xiao Linfan at SZMS.JPG|300px]] || Linfan XIAO has been nurturing her interest in life science since kindergarten age (actually it was derived from music and wildlife conservation, but that's another story). She has placed her focus on biomed, chemistry, zoology, and is now on her hitchhiking journey to the amazing galaxy of synthetic biology. In both her argumentative essays and her life, she firmly holds that the art and science of this vigorous field will prosper and thrive, contributing to the future advancement of humanity. || Theory group leader, responsible for organizing the project's theoretical construction, plasmid design, and experiment plan.<br />
|-<br />
| Ye Ziyun<br>叶子运 || [[File:Ye Ziyun at SZMS.jpg|300px]] || Ziyun Ye, a senior two student from Shenzhen Middle School, is interested in biology especially the application of biological technology. In the “Plant Sitter” project for 2014 IGEM, he takes part in plasmid construction. Although he had no experience of experiment of molecular biologybefore 2014, he tried a lot and soon got skills of simple experiment. “Just try more,” he said. || experiment<br />
|-<br />
| Liu Chenxi<br>刘宸希 || [[File:Liu Chenxi at SZMS.jpg|300px]] || Chenxi Liu, a senior two student in Shenzhen Middle School, has participated in biological study for years. Being enthusiastic on biology in younger age, she actively practices biological engineering with her teammates. In the theory group and experiment group, she works hard cooperates with others well. She believes that biological engineering is an interesting subject worth life time of hard work and research. || Theory group, experiment group <br />
|}</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T16:01:33Z<p>Peiwen cai: </p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is a high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/File:Bg.pngFile:Bg.png2014-06-20T16:00:09Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T15:55:52Z<p>Peiwen cai: </p>
<hr />
<div><body background="[[File:bg.png]]"> <br />
{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is a high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T15:55:01Z<p>Peiwen cai: </p>
<hr />
<div>{<body background="bg"> }<br />
{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is a high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T15:54:26Z<p>Peiwen cai: </p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is a high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T15:54:16Z<p>Peiwen cai: </p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is a high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T15:52:15Z<p>Peiwen cai: </p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is a high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/File:Cute_Plasmid2_.pngFile:Cute Plasmid2 .png2014-06-20T15:51:19Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/File:Cute_Plasmid3.pngFile:Cute Plasmid3.png2014-06-20T15:49:24Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/File:Cute_Plasmid5.pngFile:Cute Plasmid5.png2014-06-20T15:47:53Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/File:Cute_Plasmid8.pngFile:Cute Plasmid8.png2014-06-20T15:45:50Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/File:Cute_Plasmid6.pngFile:Cute Plasmid6.png2014-06-20T15:44:05Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/File:Cute_Plasmid7.pngFile:Cute Plasmid7.png2014-06-20T15:42:50Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/File:Cute_Plasmid4.pngFile:Cute Plasmid4.png2014-06-20T15:38:19Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T15:35:28Z<p>Peiwen cai: </p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|100px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|100px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is a high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/File:Cute_Plasmid2.pngFile:Cute Plasmid2.png2014-06-20T15:34:27Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/File:Cute_Plasmid1.pngFile:Cute Plasmid1.png2014-06-20T15:33:53Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T15:33:24Z<p>Peiwen cai: </p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|100px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|100px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is a high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMS/ProjectTeam:Shenzhen SZMS/Project2014-06-20T15:27:23Z<p>Peiwen cai: /* Background */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
----<br />
Find more about our project below!<br />
<br />
== Background ==<br />
'''E. coli Plant-sitter'''<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation. We hope that our toolkit will aid the process of plant cultivation, enlarge the public understanding of synthetic biology, and make the methods and technology of synthetic biology accessible to people’s daily life.<br />
Daily life – yes, on earth our Plant-sitter can be well applied to the growth of plants – but what about…<br />
Wait for it –<br><br />
'''MARS!'''<br><br />
[http://en.wikipedia.org/wiki/Mars Mars], lying four hundred million kilometers from earth, has the most earth-like seasons due to Mars and Earth’s similar tilts of rotational axes. As a result, in order for men to make use of Martian resources and to develop its potential as an alternative human residence in the universe, a Mars Migration Program was issued SpaceX, indicating that founding a human community on Mars requires 1) releasing carbon dioxide to make the atmosphere denser and to create raw materials for photosynthesis; '''2) cultivating plants beside human residence to generate oxygen.'''<br><br />
'''– which is where our E.coli Plant-sitter helps.'''<br />
Although there will be basic heat supply in the houses, the healthy growth of the plants is still under threat as Mars’ surface temperature varies from -143℃ to 35℃. Therefore, we came up with a system that protects the plants from overly cold environment through smart temperature control, keeping the temperature at around 27℃, aiding the plants who are burdened with the glorious purpose of generating oxygen to defend against the Jotunheim weather on Mars.<br />
<br />
<br />
Plants whose preferential temperature ranges from 25~30℃ are exposed to the threat of low productivity and unhealthy growth in overly cold environments. Therefore, we came up with a system that aids the healthy growth of these plants by protecting them from overly low temperatures through smart temperature control. Our system of temperature moderation includes two parts, temperature sensing and temperature regulating.<br />
<br />
[[File:huoxing1.jpg|200px]]<br />
[[File:huoxing3.jpg|200px]]<br />
[[File:huoxing4.jpg|200px]]<br />
[[File:huoxing2.jpg|200px]]<br />
<br />
== Plasmid and Working Details ==<br />
{| class="wikitable" align=right width=400px |+ Parts we used<br />
|-<br />
|colspan=3 align="center"|[[File:Plant-sitter temperature control Map latest version.jpg|400px|The plasmid map]]<br />
|-<br />
!width=10%| Compile !!width=30%| Function !!width=60%| Introduction <br />
|-<br />
| J23106 || Promoter || It promotes the transcription of the following parts. [http://parts.igem.org/Part:BBa_J23106 More]<br />
|-<br />
| R0051 || CI regulated Promoter || It promotes the transcription without CI. When CI is binding to it,it is repressed. [http://parts.igem.org/Part:BBa_R0051 More]<br />
|-<br />
| K115016 || 27℃ RBS || It only promotes the translation when the temperature is over 27℃ [http://parts.igem.org/Part:BBa_K115016 More]<br />
|-<br />
| K115017 || 32℃ RBS || It only promotes the translation when the temperature is over 32℃ [http://parts.igem.org/Part:BBa_K115017 More]<br />
|-<br />
| B0030 || strong RBS || It can promotes the translation strongly. [http://parts.igem.org/Part:BBa_B0030 More]<br />
|-<br />
| C0051 || Coding sequence || It is the coding sequence of CI Protein. [http://parts.igem.org/Part:BBa_C0051 More]<br />
|-<br />
| 1.7.7.2 (KEGG) || Coding sequence || We got this part from bacillus subtilis best7613 strain.It catalyzes the reaction which converts Nitrate to Nitrite. [http://www.genome.jp/dbget-bin/www_bget?ec:1.7.7.2 More]<br />
|-<br />
| K145015 || Coding scquence || It is the coding sequence of GFP. [http://parts.igem.org/Part:BBa_K145015 More]<br />
|-<br />
| B0015 || Terminator || It can stop the transcription. [http://parts.igem.org/Part:BBa_B0015 More]<br />
|}<br />
<br />
The process of temperature sensing involves a promoter, a 27℃ RNA thermometer, and cI. The process of temperature regulating involves a cI regulated promoter, an enzyme which catalyzes an exothermic chemical reaction, a 32℃ RNA thermometer, and a GFP LVA device which serves to indicate the occurrence of a change in temperature. The promoter in temperature sensing part is consistently on and thus so is the process of cI’s transcription from DNA to RNA. When the temperature is below 27℃, the 27℃RNA thermometer takes a curved, locked structure which does not enable translation from RNA to protein cI, which in turn allows the activation of the cI regulated promoter, the production of the enzyme, the occurrence of an exothermic reaction which heats up the environment, and the expression of GFP LVA tag which gives out green light to indicate that the temperature is below 27℃ and that our device of temperature control is doing its work. When the temperature rises and reaches 27 ℃ as a result of the exothermic reaction, the 27℃ RNA thermometer enables the translation of cI protein, which in turn represses the cI regulated promoter, ending the release of heat through the exothermic reaction by stopping the production of the enzyme which catalyzes that reaction. As a result of an inactive promoter (the cI regulated), the GFP also stops its expression and is decomposes, indicating that the temperature has risen from below 27 ℃ to 27 ℃and that our system of temperature control has done its work.<br />
If the 27℃ RNA thermometer fails to stop the heating process at around 27℃ and the temperature keeps rising up to 32℃, the 32℃ RNA thermometer is activated, leading to the presence of protein cI, which places a double-check on the inhibition of the heating process.<br />
<br />
=== Temperature Sensing ===<br />
This process involves BBa_J23106 (promoter), BBa_K115016 (27℃ RNA thermometer), and cI (Repressor protein cI).<br />
BBa_J23106 is consistently on and thus so is the process of cI’s transcription from DNA to RNA. When the temperature is below 27℃, <br />
the 27℃ sensitive RNA thermometer does not enable translation from RNA to protein cI, which in turn allows the activation of BBa_R0051, the production of the enzyme, the occurrence of an exothermic reaction which heats up the environment, <br />
and the expression of GFP LVA tag which gives out green light to indicate that the temperature is below 27℃ and that our device of temperature control is doing its work.<br />
<br />
When the temperature rises and reaches 27 ℃ as a result of the exothermic reaction, BBa_K115016 (27℃ RNA thermometer) enables the translation of cI protein, which in turn represses BBa_R0051, <br />
ending the release of heat through the exothermic reaction by stopping the production of the enzyme which catalyzes that reaction.<br />
<br />
As a result of an inactive promoter (BBa_R0051), the GFP also stops its expression and is decomposes,<br />
indicating that the temperature has risen from below 27 ℃ to 27 ℃and that our system of temperature control has done its work.<br />
<br />
If BBa_K115016 (27℃ RNA thermometer) fails to stop the heating process at around 27℃ and the temperature keeps rising up to 32℃, BBa_K115017 (32℃ RNA thermometer) is activated, leading to the presence of protein cI, <br />
which places a double-check on the inhibition of the heating process.<br />
<br />
The process by which our temperature-control system operates may be better illustrated in the following flow chart:<br />
<br />
=== Temperature Regulating ===<br />
The process of temperature regulating involves BBa_J45503 (cI regulated promoter), an enzyme which catalyzes an exothermic chemical reaction, BBa_K115017 (32℃ RNA thermometer) and a GFP LVA device which serves to indicate the occurrence of a change in temperature.<br />
<br style="clear:both;"><br />
----<br />
The following is a flowchart explaining how our E. coli Plant-sitter works.<br />
<br />
[[File:IGem_-_New_Page_Correcto.png|center|900px]]</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMS/ProjectTeam:Shenzhen SZMS/Project2014-06-20T15:26:10Z<p>Peiwen cai: </p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
----<br />
Find more about our project below!<br />
<br />
== Background ==<br />
'''E. coli Plant-sitter'''<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation. We hope that our toolkit will aid the process of plant cultivation, enlarge the public understanding of synthetic biology, and make the methods and technology of synthetic biology accessible to people’s daily life.<br />
Daily life – yes, on earth our Plant-sitter can be well applied to the growth of plants – but what about…<br />
Wait for it –<br />
'''MARS!'''<br />
[http://en.wikipedia.org/wiki/Mars Mars], lying four hundred million kilometers from earth, has the most earth-like seasons due to Mars and Earth’s similar tilts of rotational axes. As a result, in order for men to make use of Martian resources and to develop its potential as an alternative human residence in the universe, a Mars Migration Program was issued SpaceX, indicating that founding a human community on Mars requires 1) releasing carbon dioxide to make the atmosphere denser and to create raw materials for photosynthesis; '''2) cultivating plants beside human residence to generate oxygen.'''<br><br />
'''– which is where our E.coli Plant-sitter helps.'''<br />
Although there will be basic heat supply in the houses, the healthy growth of the plants is still under threat as Mars’ surface temperature varies from -143℃ to 35℃. Therefore, we came up with a system that protects the plants from overly cold environment through smart temperature control, keeping the temperature at around 27℃, aiding the plants who are burdened with the glorious purpose of generating oxygen to defend against the Jotunheim weather on Mars.<br />
<br />
<br />
Plants whose preferential temperature ranges from 25~30℃ are exposed to the threat of low productivity and unhealthy growth in overly cold environments. Therefore, we came up with a system that aids the healthy growth of these plants by protecting them from overly low temperatures through smart temperature control. Our system of temperature moderation includes two parts, temperature sensing and temperature regulating.<br />
<br />
[[File:huoxing1.jpg|200px]]<br />
[[File:huoxing3.jpg|200px]]<br />
[[File:huoxing4.jpg|200px]]<br />
[[File:huoxing2.jpg|200px]]<br />
<br />
== Plasmid and Working Details ==<br />
{| class="wikitable" align=right width=400px |+ Parts we used<br />
|-<br />
|colspan=3 align="center"|[[File:Plant-sitter temperature control Map latest version.jpg|400px|The plasmid map]]<br />
|-<br />
!width=10%| Compile !!width=30%| Function !!width=60%| Introduction <br />
|-<br />
| J23106 || Promoter || It promotes the transcription of the following parts. [http://parts.igem.org/Part:BBa_J23106 More]<br />
|-<br />
| R0051 || CI regulated Promoter || It promotes the transcription without CI. When CI is binding to it,it is repressed. [http://parts.igem.org/Part:BBa_R0051 More]<br />
|-<br />
| K115016 || 27℃ RBS || It only promotes the translation when the temperature is over 27℃ [http://parts.igem.org/Part:BBa_K115016 More]<br />
|-<br />
| K115017 || 32℃ RBS || It only promotes the translation when the temperature is over 32℃ [http://parts.igem.org/Part:BBa_K115017 More]<br />
|-<br />
| B0030 || strong RBS || It can promotes the translation strongly. [http://parts.igem.org/Part:BBa_B0030 More]<br />
|-<br />
| C0051 || Coding sequence || It is the coding sequence of CI Protein. [http://parts.igem.org/Part:BBa_C0051 More]<br />
|-<br />
| 1.7.7.2 (KEGG) || Coding sequence || We got this part from bacillus subtilis best7613 strain.It catalyzes the reaction which converts Nitrate to Nitrite. [http://www.genome.jp/dbget-bin/www_bget?ec:1.7.7.2 More]<br />
|-<br />
| K145015 || Coding scquence || It is the coding sequence of GFP. [http://parts.igem.org/Part:BBa_K145015 More]<br />
|-<br />
| B0015 || Terminator || It can stop the transcription. [http://parts.igem.org/Part:BBa_B0015 More]<br />
|}<br />
<br />
The process of temperature sensing involves a promoter, a 27℃ RNA thermometer, and cI. The process of temperature regulating involves a cI regulated promoter, an enzyme which catalyzes an exothermic chemical reaction, a 32℃ RNA thermometer, and a GFP LVA device which serves to indicate the occurrence of a change in temperature. The promoter in temperature sensing part is consistently on and thus so is the process of cI’s transcription from DNA to RNA. When the temperature is below 27℃, the 27℃RNA thermometer takes a curved, locked structure which does not enable translation from RNA to protein cI, which in turn allows the activation of the cI regulated promoter, the production of the enzyme, the occurrence of an exothermic reaction which heats up the environment, and the expression of GFP LVA tag which gives out green light to indicate that the temperature is below 27℃ and that our device of temperature control is doing its work. When the temperature rises and reaches 27 ℃ as a result of the exothermic reaction, the 27℃ RNA thermometer enables the translation of cI protein, which in turn represses the cI regulated promoter, ending the release of heat through the exothermic reaction by stopping the production of the enzyme which catalyzes that reaction. As a result of an inactive promoter (the cI regulated), the GFP also stops its expression and is decomposes, indicating that the temperature has risen from below 27 ℃ to 27 ℃and that our system of temperature control has done its work.<br />
If the 27℃ RNA thermometer fails to stop the heating process at around 27℃ and the temperature keeps rising up to 32℃, the 32℃ RNA thermometer is activated, leading to the presence of protein cI, which places a double-check on the inhibition of the heating process.<br />
<br />
=== Temperature Sensing ===<br />
This process involves BBa_J23106 (promoter), BBa_K115016 (27℃ RNA thermometer), and cI (Repressor protein cI).<br />
BBa_J23106 is consistently on and thus so is the process of cI’s transcription from DNA to RNA. When the temperature is below 27℃, <br />
the 27℃ sensitive RNA thermometer does not enable translation from RNA to protein cI, which in turn allows the activation of BBa_R0051, the production of the enzyme, the occurrence of an exothermic reaction which heats up the environment, <br />
and the expression of GFP LVA tag which gives out green light to indicate that the temperature is below 27℃ and that our device of temperature control is doing its work.<br />
<br />
When the temperature rises and reaches 27 ℃ as a result of the exothermic reaction, BBa_K115016 (27℃ RNA thermometer) enables the translation of cI protein, which in turn represses BBa_R0051, <br />
ending the release of heat through the exothermic reaction by stopping the production of the enzyme which catalyzes that reaction.<br />
<br />
As a result of an inactive promoter (BBa_R0051), the GFP also stops its expression and is decomposes,<br />
indicating that the temperature has risen from below 27 ℃ to 27 ℃and that our system of temperature control has done its work.<br />
<br />
If BBa_K115016 (27℃ RNA thermometer) fails to stop the heating process at around 27℃ and the temperature keeps rising up to 32℃, BBa_K115017 (32℃ RNA thermometer) is activated, leading to the presence of protein cI, <br />
which places a double-check on the inhibition of the heating process.<br />
<br />
The process by which our temperature-control system operates may be better illustrated in the following flow chart:<br />
<br />
=== Temperature Regulating ===<br />
The process of temperature regulating involves BBa_J45503 (cI regulated promoter), an enzyme which catalyzes an exothermic chemical reaction, BBa_K115017 (32℃ RNA thermometer) and a GFP LVA device which serves to indicate the occurrence of a change in temperature.<br />
<br style="clear:both;"><br />
----<br />
The following is a flowchart explaining how our E. coli Plant-sitter works.<br />
<br />
[[File:IGem_-_New_Page_Correcto.png|center|900px]]</div>Peiwen caihttp://2014hs.igem.org/File:IGem_-_New_Page_Correcto.pngFile:IGem - New Page Correcto.png2014-06-20T15:25:06Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMS/ProjectTeam:Shenzhen SZMS/Project2014-06-20T15:24:19Z<p>Peiwen cai: /* Temperature Regulating */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
----<br />
Find more of our project below!<br />
<br />
== Background ==<br />
'''E. coli Plant-sitter'''<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation. We hope that our toolkit will aid the process of plant cultivation, enlarge the public understanding of synthetic biology, and make the methods and technology of synthetic biology accessible to people’s daily life.<br />
Daily life – yes, on earth our Plant-sitter can be well applied to the growth of plants – but what about…<br />
Wait for it –<br />
'''MARS!'''<br />
[http://en.wikipedia.org/wiki/Mars Mars], lying four hundred million kilometers from earth, has the most earth-like seasons due to Mars and Earth’s similar tilts of rotational axes. As a result, in order for men to make use of Martian resources and to develop its potential as an alternative human residence in the universe, a Mars Migration Program was issued SpaceX, indicating that founding a human community on Mars requires 1) releasing carbon dioxide to make the atmosphere denser and to create raw materials for photosynthesis; '''2) cultivating plants beside human residence to generate oxygen.'''<br><br />
'''– which is where our E.coli Plant-sitter helps.'''<br />
Although there will be basic heat supply in the houses, the healthy growth of the plants is still under threat as Mars’ surface temperature varies from -143℃ to 35℃. Therefore, we came up with a system that protects the plants from overly cold environment through smart temperature control, keeping the temperature at around 27℃, aiding the plants who are burdened with the glorious purpose of generating oxygen to defend against the Jotunheim weather on Mars.<br />
<br />
<br />
Plants whose preferential temperature ranges from 25~30℃ are exposed to the threat of low productivity and unhealthy growth in overly cold environments. Therefore, we came up with a system that aids the healthy growth of these plants by protecting them from overly low temperatures through smart temperature control. Our system of temperature moderation includes two parts, temperature sensing and temperature regulating.<br />
<br />
[[File:huoxing1.jpg|200px]]<br />
[[File:huoxing3.jpg|200px]]<br />
[[File:huoxing4.jpg|200px]]<br />
[[File:huoxing2.jpg|200px]]<br />
<br />
== Plasmid and Working Details ==<br />
{| class="wikitable" align=right width=400px |+ Parts we used<br />
|-<br />
|colspan=3 align="center"|[[File:Plant-sitter temperature control Map latest version.jpg|400px|The plasmid map]]<br />
|-<br />
!width=10%| Compile !!width=30%| Function !!width=60%| Introduction <br />
|-<br />
| J23106 || Promoter || It promotes the transcription of the following parts. [http://parts.igem.org/Part:BBa_J23106 More]<br />
|-<br />
| R0051 || CI regulated Promoter || It promotes the transcription without CI. When CI is binding to it,it is repressed. [http://parts.igem.org/Part:BBa_R0051 More]<br />
|-<br />
| K115016 || 27℃ RBS || It only promotes the translation when the temperature is over 27℃ [http://parts.igem.org/Part:BBa_K115016 More]<br />
|-<br />
| K115017 || 32℃ RBS || It only promotes the translation when the temperature is over 32℃ [http://parts.igem.org/Part:BBa_K115017 More]<br />
|-<br />
| B0030 || strong RBS || It can promotes the translation strongly. [http://parts.igem.org/Part:BBa_B0030 More]<br />
|-<br />
| C0051 || Coding sequence || It is the coding sequence of CI Protein. [http://parts.igem.org/Part:BBa_C0051 More]<br />
|-<br />
| 1.7.7.2 (KEGG) || Coding sequence || We got this part from bacillus subtilis best7613 strain.It catalyzes the reaction which converts Nitrate to Nitrite. [http://www.genome.jp/dbget-bin/www_bget?ec:1.7.7.2 More]<br />
|-<br />
| K145015 || Coding scquence || It is the coding sequence of GFP. [http://parts.igem.org/Part:BBa_K145015 More]<br />
|-<br />
| B0015 || Terminator || It can stop the transcription. [http://parts.igem.org/Part:BBa_B0015 More]<br />
|}<br />
<br />
The process of temperature sensing involves a promoter, a 27℃ RNA thermometer, and cI. The process of temperature regulating involves a cI regulated promoter, an enzyme which catalyzes an exothermic chemical reaction, a 32℃ RNA thermometer, and a GFP LVA device which serves to indicate the occurrence of a change in temperature. The promoter in temperature sensing part is consistently on and thus so is the process of cI’s transcription from DNA to RNA. When the temperature is below 27℃, the 27℃RNA thermometer takes a curved, locked structure which does not enable translation from RNA to protein cI, which in turn allows the activation of the cI regulated promoter, the production of the enzyme, the occurrence of an exothermic reaction which heats up the environment, and the expression of GFP LVA tag which gives out green light to indicate that the temperature is below 27℃ and that our device of temperature control is doing its work. When the temperature rises and reaches 27 ℃ as a result of the exothermic reaction, the 27℃ RNA thermometer enables the translation of cI protein, which in turn represses the cI regulated promoter, ending the release of heat through the exothermic reaction by stopping the production of the enzyme which catalyzes that reaction. As a result of an inactive promoter (the cI regulated), the GFP also stops its expression and is decomposes, indicating that the temperature has risen from below 27 ℃ to 27 ℃and that our system of temperature control has done its work.<br />
If the 27℃ RNA thermometer fails to stop the heating process at around 27℃ and the temperature keeps rising up to 32℃, the 32℃ RNA thermometer is activated, leading to the presence of protein cI, which places a double-check on the inhibition of the heating process.<br />
<br />
=== Temperature Sensing ===<br />
This process involves BBa_J23106 (promoter), BBa_K115016 (27℃ RNA thermometer), and cI (Repressor protein cI).<br />
BBa_J23106 is consistently on and thus so is the process of cI’s transcription from DNA to RNA. When the temperature is below 27℃, <br />
the 27℃ sensitive RNA thermometer does not enable translation from RNA to protein cI, which in turn allows the activation of BBa_R0051, the production of the enzyme, the occurrence of an exothermic reaction which heats up the environment, <br />
and the expression of GFP LVA tag which gives out green light to indicate that the temperature is below 27℃ and that our device of temperature control is doing its work.<br />
<br />
When the temperature rises and reaches 27 ℃ as a result of the exothermic reaction, BBa_K115016 (27℃ RNA thermometer) enables the translation of cI protein, which in turn represses BBa_R0051, <br />
ending the release of heat through the exothermic reaction by stopping the production of the enzyme which catalyzes that reaction.<br />
<br />
As a result of an inactive promoter (BBa_R0051), the GFP also stops its expression and is decomposes,<br />
indicating that the temperature has risen from below 27 ℃ to 27 ℃and that our system of temperature control has done its work.<br />
<br />
If BBa_K115016 (27℃ RNA thermometer) fails to stop the heating process at around 27℃ and the temperature keeps rising up to 32℃, BBa_K115017 (32℃ RNA thermometer) is activated, leading to the presence of protein cI, <br />
which places a double-check on the inhibition of the heating process.<br />
<br />
The process by which our temperature-control system operates may be better illustrated in the following flow chart:<br />
<br />
=== Temperature Regulating ===<br />
The process of temperature regulating involves BBa_J45503 (cI regulated promoter), an enzyme which catalyzes an exothermic chemical reaction, BBa_K115017 (32℃ RNA thermometer) and a GFP LVA device which serves to indicate the occurrence of a change in temperature.<br />
<br style="clear:both;"><br />
----<br />
The following is a flowchart explaining how our E. coli Plant-sitter works.<br />
<br />
[[File:IGem_-_New_Page_Correcto.png|center|900px]]</div>Peiwen caihttp://2014hs.igem.org/File:Plant-sitter_temperature_control_Map_latest_version.jpgFile:Plant-sitter temperature control Map latest version.jpg2014-06-20T15:21:05Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMS/ProjectTeam:Shenzhen SZMS/Project2014-06-20T15:20:40Z<p>Peiwen cai: /* Plasmid and Working Details */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
----<br />
Find more of our project below!<br />
<br />
== Background ==<br />
'''E. coli Plant-sitter'''<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation. We hope that our toolkit will aid the process of plant cultivation, enlarge the public understanding of synthetic biology, and make the methods and technology of synthetic biology accessible to people’s daily life.<br />
Daily life – yes, on earth our Plant-sitter can be well applied to the growth of plants – but what about…<br />
Wait for it –<br />
'''MARS!'''<br />
[http://en.wikipedia.org/wiki/Mars Mars], lying four hundred million kilometers from earth, has the most earth-like seasons due to Mars and Earth’s similar tilts of rotational axes. As a result, in order for men to make use of Martian resources and to develop its potential as an alternative human residence in the universe, a Mars Migration Program was issued SpaceX, indicating that founding a human community on Mars requires 1) releasing carbon dioxide to make the atmosphere denser and to create raw materials for photosynthesis; '''2) cultivating plants beside human residence to generate oxygen.'''<br><br />
'''– which is where our E.coli Plant-sitter helps.'''<br />
Although there will be basic heat supply in the houses, the healthy growth of the plants is still under threat as Mars’ surface temperature varies from -143℃ to 35℃. Therefore, we came up with a system that protects the plants from overly cold environment through smart temperature control, keeping the temperature at around 27℃, aiding the plants who are burdened with the glorious purpose of generating oxygen to defend against the Jotunheim weather on Mars.<br />
<br />
<br />
Plants whose preferential temperature ranges from 25~30℃ are exposed to the threat of low productivity and unhealthy growth in overly cold environments. Therefore, we came up with a system that aids the healthy growth of these plants by protecting them from overly low temperatures through smart temperature control. Our system of temperature moderation includes two parts, temperature sensing and temperature regulating.<br />
<br />
[[File:huoxing1.jpg|200px]]<br />
[[File:huoxing3.jpg|200px]]<br />
[[File:huoxing4.jpg|200px]]<br />
[[File:huoxing2.jpg|200px]]<br />
<br />
== Plasmid and Working Details ==<br />
{| class="wikitable" align=right width=400px |+ Parts we used<br />
|-<br />
|colspan=3 align="center"|[[File:Plant-sitter temperature control Map latest version.jpg|400px|The plasmid map]]<br />
|-<br />
!width=10%| Compile !!width=30%| Function !!width=60%| Introduction <br />
|-<br />
| J23106 || Promoter || It promotes the transcription of the following parts. [http://parts.igem.org/Part:BBa_J23106 More]<br />
|-<br />
| R0051 || CI regulated Promoter || It promotes the transcription without CI. When CI is binding to it,it is repressed. [http://parts.igem.org/Part:BBa_R0051 More]<br />
|-<br />
| K115016 || 27℃ RBS || It only promotes the translation when the temperature is over 27℃ [http://parts.igem.org/Part:BBa_K115016 More]<br />
|-<br />
| K115017 || 32℃ RBS || It only promotes the translation when the temperature is over 32℃ [http://parts.igem.org/Part:BBa_K115017 More]<br />
|-<br />
| B0030 || strong RBS || It can promotes the translation strongly. [http://parts.igem.org/Part:BBa_B0030 More]<br />
|-<br />
| C0051 || Coding sequence || It is the coding sequence of CI Protein. [http://parts.igem.org/Part:BBa_C0051 More]<br />
|-<br />
| 1.7.7.2 (KEGG) || Coding sequence || We got this part from bacillus subtilis best7613 strain.It catalyzes the reaction which converts Nitrate to Nitrite. [http://www.genome.jp/dbget-bin/www_bget?ec:1.7.7.2 More]<br />
|-<br />
| K145015 || Coding scquence || It is the coding sequence of GFP. [http://parts.igem.org/Part:BBa_K145015 More]<br />
|-<br />
| B0015 || Terminator || It can stop the transcription. [http://parts.igem.org/Part:BBa_B0015 More]<br />
|}<br />
<br />
The process of temperature sensing involves a promoter, a 27℃ RNA thermometer, and cI. The process of temperature regulating involves a cI regulated promoter, an enzyme which catalyzes an exothermic chemical reaction, a 32℃ RNA thermometer, and a GFP LVA device which serves to indicate the occurrence of a change in temperature. The promoter in temperature sensing part is consistently on and thus so is the process of cI’s transcription from DNA to RNA. When the temperature is below 27℃, the 27℃RNA thermometer takes a curved, locked structure which does not enable translation from RNA to protein cI, which in turn allows the activation of the cI regulated promoter, the production of the enzyme, the occurrence of an exothermic reaction which heats up the environment, and the expression of GFP LVA tag which gives out green light to indicate that the temperature is below 27℃ and that our device of temperature control is doing its work. When the temperature rises and reaches 27 ℃ as a result of the exothermic reaction, the 27℃ RNA thermometer enables the translation of cI protein, which in turn represses the cI regulated promoter, ending the release of heat through the exothermic reaction by stopping the production of the enzyme which catalyzes that reaction. As a result of an inactive promoter (the cI regulated), the GFP also stops its expression and is decomposes, indicating that the temperature has risen from below 27 ℃ to 27 ℃and that our system of temperature control has done its work.<br />
If the 27℃ RNA thermometer fails to stop the heating process at around 27℃ and the temperature keeps rising up to 32℃, the 32℃ RNA thermometer is activated, leading to the presence of protein cI, which places a double-check on the inhibition of the heating process.<br />
<br />
=== Temperature Sensing ===<br />
This process involves BBa_J23106 (promoter), BBa_K115016 (27℃ RNA thermometer), and cI (Repressor protein cI).<br />
BBa_J23106 is consistently on and thus so is the process of cI’s transcription from DNA to RNA. When the temperature is below 27℃, <br />
the 27℃ sensitive RNA thermometer does not enable translation from RNA to protein cI, which in turn allows the activation of BBa_R0051, the production of the enzyme, the occurrence of an exothermic reaction which heats up the environment, <br />
and the expression of GFP LVA tag which gives out green light to indicate that the temperature is below 27℃ and that our device of temperature control is doing its work.<br />
<br />
When the temperature rises and reaches 27 ℃ as a result of the exothermic reaction, BBa_K115016 (27℃ RNA thermometer) enables the translation of cI protein, which in turn represses BBa_R0051, <br />
ending the release of heat through the exothermic reaction by stopping the production of the enzyme which catalyzes that reaction.<br />
<br />
As a result of an inactive promoter (BBa_R0051), the GFP also stops its expression and is decomposes,<br />
indicating that the temperature has risen from below 27 ℃ to 27 ℃and that our system of temperature control has done its work.<br />
<br />
If BBa_K115016 (27℃ RNA thermometer) fails to stop the heating process at around 27℃ and the temperature keeps rising up to 32℃, BBa_K115017 (32℃ RNA thermometer) is activated, leading to the presence of protein cI, <br />
which places a double-check on the inhibition of the heating process.<br />
<br />
The process by which our temperature-control system operates may be better illustrated in the following flow chart:<br />
<br />
=== Temperature Regulating ===<br />
The process of temperature regulating involves BBa_J45503 (cI regulated promoter), an enzyme which catalyzes an exothermic chemical reaction, BBa_K115017 (32℃ RNA thermometer) and a GFP LVA device which serves to indicate the occurrence of a change in temperature.<br />
<br style="clear:both;"><br />
----<br />
The following is a flowchart explaining how our E. coli Plant-sitter works.<br />
<br />
[[File:IGem_-_New_Page.png|center|900px]]</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMS/ProjectTeam:Shenzhen SZMS/Project2014-06-20T15:19:46Z<p>Peiwen cai: /* Background */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
----<br />
Find more of our project below!<br />
<br />
== Background ==<br />
'''E. coli Plant-sitter'''<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation. We hope that our toolkit will aid the process of plant cultivation, enlarge the public understanding of synthetic biology, and make the methods and technology of synthetic biology accessible to people’s daily life.<br />
Daily life – yes, on earth our Plant-sitter can be well applied to the growth of plants – but what about…<br />
Wait for it –<br />
'''MARS!'''<br />
[http://en.wikipedia.org/wiki/Mars Mars], lying four hundred million kilometers from earth, has the most earth-like seasons due to Mars and Earth’s similar tilts of rotational axes. As a result, in order for men to make use of Martian resources and to develop its potential as an alternative human residence in the universe, a Mars Migration Program was issued SpaceX, indicating that founding a human community on Mars requires 1) releasing carbon dioxide to make the atmosphere denser and to create raw materials for photosynthesis; '''2) cultivating plants beside human residence to generate oxygen.'''<br><br />
'''– which is where our E.coli Plant-sitter helps.'''<br />
Although there will be basic heat supply in the houses, the healthy growth of the plants is still under threat as Mars’ surface temperature varies from -143℃ to 35℃. Therefore, we came up with a system that protects the plants from overly cold environment through smart temperature control, keeping the temperature at around 27℃, aiding the plants who are burdened with the glorious purpose of generating oxygen to defend against the Jotunheim weather on Mars.<br />
<br />
<br />
Plants whose preferential temperature ranges from 25~30℃ are exposed to the threat of low productivity and unhealthy growth in overly cold environments. Therefore, we came up with a system that aids the healthy growth of these plants by protecting them from overly low temperatures through smart temperature control. Our system of temperature moderation includes two parts, temperature sensing and temperature regulating.<br />
<br />
[[File:huoxing1.jpg|200px]]<br />
[[File:huoxing3.jpg|200px]]<br />
[[File:huoxing4.jpg|200px]]<br />
[[File:huoxing2.jpg|200px]]<br />
<br />
== Plasmid and Working Details ==<br />
{| class="wikitable" align=right width=400px |+ Parts we used<br />
|-<br />
|colspan=3 align="center"|[[File:Plant-sitter temperature control Map.jpg|400px|The plasmid map]]<br />
|-<br />
!width=10%| Compile !!width=30%| Function !!width=60%| Introduction <br />
|-<br />
| J23106 || Promoter || It promotes the transcription of the following parts. [http://parts.igem.org/Part:BBa_J23106 More]<br />
|-<br />
| R0051 || CI regulated Promoter || It promotes the transcription without CI. When CI is binding to it,it is repressed. [http://parts.igem.org/Part:BBa_R0051 More]<br />
|-<br />
| K115016 || 27℃ RBS || It only promotes the translation when the temperature is over 27℃ [http://parts.igem.org/Part:BBa_K115016 More]<br />
|-<br />
| K115017 || 32℃ RBS || It only promotes the translation when the temperature is over 32℃ [http://parts.igem.org/Part:BBa_K115017 More]<br />
|-<br />
| B0030 || strong RBS || It can promotes the translation strongly. [http://parts.igem.org/Part:BBa_B0030 More]<br />
|-<br />
| C0051 || Coding sequence || It is the coding sequence of CI Protein. [http://parts.igem.org/Part:BBa_C0051 More]<br />
|-<br />
| 1.7.7.2 (KEGG) || Coding sequence || We got this part from bacillus subtilis best7613 strain.It catalyzes the reaction which converts Nitrate to Nitrite. [http://www.genome.jp/dbget-bin/www_bget?ec:1.7.7.2 More]<br />
|-<br />
| K145015 || Coding scquence || It is the coding sequence of GFP. [http://parts.igem.org/Part:BBa_K145015 More]<br />
|-<br />
| B0015 || Terminator || It can stop the transcription. [http://parts.igem.org/Part:BBa_B0015 More]<br />
|}<br />
<br />
The process of temperature sensing involves a promoter, a 27℃ RNA thermometer, and cI. The process of temperature regulating involves a cI regulated promoter, an enzyme which catalyzes an exothermic chemical reaction, a 32℃ RNA thermometer, and a GFP LVA device which serves to indicate the occurrence of a change in temperature. The promoter in temperature sensing part is consistently on and thus so is the process of cI’s transcription from DNA to RNA. When the temperature is below 27℃, the 27℃RNA thermometer takes a curved, locked structure which does not enable translation from RNA to protein cI, which in turn allows the activation of the cI regulated promoter, the production of the enzyme, the occurrence of an exothermic reaction which heats up the environment, and the expression of GFP LVA tag which gives out green light to indicate that the temperature is below 27℃ and that our device of temperature control is doing its work. When the temperature rises and reaches 27 ℃ as a result of the exothermic reaction, the 27℃ RNA thermometer enables the translation of cI protein, which in turn represses the cI regulated promoter, ending the release of heat through the exothermic reaction by stopping the production of the enzyme which catalyzes that reaction. As a result of an inactive promoter (the cI regulated), the GFP also stops its expression and is decomposes, indicating that the temperature has risen from below 27 ℃ to 27 ℃and that our system of temperature control has done its work.<br />
If the 27℃ RNA thermometer fails to stop the heating process at around 27℃ and the temperature keeps rising up to 32℃, the 32℃ RNA thermometer is activated, leading to the presence of protein cI, which places a double-check on the inhibition of the heating process.<br />
<br />
=== Temperature Sensing ===<br />
This process involves BBa_J23106 (promoter), BBa_K115016 (27℃ RNA thermometer), and cI (Repressor protein cI).<br />
BBa_J23106 is consistently on and thus so is the process of cI’s transcription from DNA to RNA. When the temperature is below 27℃, <br />
the 27℃ sensitive RNA thermometer does not enable translation from RNA to protein cI, which in turn allows the activation of BBa_R0051, the production of the enzyme, the occurrence of an exothermic reaction which heats up the environment, <br />
and the expression of GFP LVA tag which gives out green light to indicate that the temperature is below 27℃ and that our device of temperature control is doing its work.<br />
<br />
When the temperature rises and reaches 27 ℃ as a result of the exothermic reaction, BBa_K115016 (27℃ RNA thermometer) enables the translation of cI protein, which in turn represses BBa_R0051, <br />
ending the release of heat through the exothermic reaction by stopping the production of the enzyme which catalyzes that reaction.<br />
<br />
As a result of an inactive promoter (BBa_R0051), the GFP also stops its expression and is decomposes,<br />
indicating that the temperature has risen from below 27 ℃ to 27 ℃and that our system of temperature control has done its work.<br />
<br />
If BBa_K115016 (27℃ RNA thermometer) fails to stop the heating process at around 27℃ and the temperature keeps rising up to 32℃, BBa_K115017 (32℃ RNA thermometer) is activated, leading to the presence of protein cI, <br />
which places a double-check on the inhibition of the heating process.<br />
<br />
The process by which our temperature-control system operates may be better illustrated in the following flow chart:<br />
<br />
=== Temperature Regulating ===<br />
The process of temperature regulating involves BBa_J45503 (cI regulated promoter), an enzyme which catalyzes an exothermic chemical reaction, BBa_K115017 (32℃ RNA thermometer) and a GFP LVA device which serves to indicate the occurrence of a change in temperature.<br />
<br style="clear:both;"><br />
----<br />
The following is a flowchart explaining how our E. coli Plant-sitter works.<br />
<br />
[[File:IGem_-_New_Page.png|center|900px]]</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T15:17:20Z<p>Peiwen cai: /* Project */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=12.5%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
[https://2014hs.igem.org/Main_Page <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is a high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/File:Yang_Yuwei_meizhaopian.JPGFile:Yang Yuwei meizhaopian.JPG2014-06-20T13:31:44Z<p>Peiwen cai: uploaded a new version of &quot;File:Yang Yuwei meizhaopian.JPG&quot;</p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/File:Yang_Yuwei_meizhaopian.JPGFile:Yang Yuwei meizhaopian.JPG2014-06-20T13:28:25Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen cai