http://2014hs.igem.org/wiki/index.php?title=Special:Contributions&feed=atom&limit=50&target=Kahanpparekh2014hs.igem.org - User contributions [en]2024-03-29T15:03:32ZFrom 2014hs.igem.orgMediaWiki 1.16.5http://2014hs.igem.org/Team:Lambert_GA/NoteBookTeam:Lambert GA/NoteBook2014-06-15T20:28:57Z<p>Kahanpparekh: </p>
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Lambert iGEM NoteBook<br />
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<h1>Lambert iGEM NoteBook</h1><br />
<p><br />
Jared Cook still has to upload his Notebook.<br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/FunTeam:Lambert GA/Fun2014-06-15T20:28:44Z<p>Kahanpparekh: </p>
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<h1>Lambert iGEM Fun</h1><br />
<h2>Video</h2><br />
<p><br />
Mrs. Standeven still has to complete the video. I will embed it as soon as she finishes it.<br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/HumanPracticesTeam:Lambert GA/HumanPractices2014-06-15T20:28:32Z<p>Kahanpparekh: </p>
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Lambert iGEM Human Practices (Outreach)<br />
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<h1>Lambert iGEM Human Practices (Outreach)</h1><br />
<h2>NGF Tutoring:</h2><br />
<p><br />
...<br />
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<h2>Whitlow Elemtary:</h2><br />
<p><br />
As part of iGEM’s goal to expand the ideas of biotechnology in the community, a group of iGEM students participated at the annual STEM Night at Whitlow Elementary. Here a diverse collection of subjects were discussed and iGEM was able to demonstrate common biotechnology applications to young children. The children were amazed and demonstrated curiosity in the synthetic biotechnology field. We demonstrated how a micropipette is used and the miniscule volume that is measured by them. Furthermore, a gel electrophoresis was set up to show some of the unique apparatus we work with. The parents were able to gain an understanding of the objectives of iGEM. In addition, iGEM garnered recognition in the community for a well-developed club that was able to accomplish admirable feats in just a year of its inauguration.<br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/SafetyTeam:Lambert GA/Safety2014-06-15T20:28:19Z<p>Kahanpparekh: </p>
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<h1>Lambert iGEM Safety</h1><br />
<ul> How We Lab Safely<br />
<li> <b>ALL</b> team members were trained in appropriate techniques for dealing with cell culture, recombinant DNA, and general lab safety.</li><br />
<li> <b>ALL</b> lab procedures were done under the supervision of the instructors.</li><br />
<li> <b>ALL</b> experiments with recombinant DNA were approved by Dr. Mark Styczynski from the Georgia Institute of Technology.</li><br />
<li> <b>ALL</b> disposal of lab waste was treated in accordance with the MSDS or appropriate sterilization of biohazards.</li><br />
</ul><br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/TeamTeam:Lambert GA/Team2014-06-15T20:28:06Z<p>Kahanpparekh: </p>
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Lambert iGEM Team<br />
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<h1>Lambert iGEM Team</h1><br />
<p><br />
This Page Requires Image Uploads. Will Finish Later.<br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/SponsorsTeam:Lambert GA/Sponsors2014-06-15T20:27:49Z<p>Kahanpparekh: </p>
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Lambert iGEM Sponsors<br />
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<h1>Lambert iGEM Sponsors</h1><br />
<h2>Ge Family</h2><br />
<h2><a href="http://www.chick-fil-a.com/johnscreek" target="_blank">John's Creek Chikfila</a></h2><br />
<h2><a href="https://www.neb.com/?gclid=CNzq17vG_L4CFRJk7Aodi3gAuA" target="_blank">New England BioLabs</a></h2><br />
<h2><a href="http://www.nsf.gov/awardsearch/showAward?AWD_ID=1254382" target="_blank">National Science Foundation Grant #1254382</a></h2><br />
<h2><a href="http://chbe.gatech.edu/faculty/styczynski" target="_blank">The Styczynski Lab at Georgia Institue of Technology</a></h2><br />
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</div><br />
</body><br />
</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/ProjectTeam:Lambert GA/Project2014-06-15T20:27:26Z<p>Kahanpparekh: </p>
<hr />
<div><html><br />
<head><br />
<title><br />
Lambert iGEM Project<br />
</title><br />
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<h1>Lambert iGEM Project</h1><br />
<h2>Biobricks:</h2><br />
<p><br />
Biobricks are defined as the “…standard for interchangeable parts, developed with a view to building biological systems in living cells” by the iGEM Parts website. Our contribution to CDA would be essential to biobrick because it will allow others to use the part in the future. The biobrick that is created will be mapped out and put into a database which is basically a free dictionary of biobricks. This compilation of biobricks allow others to use them in their experiments, expand on, or restructure. Without a database of biobricks, we would have a hard time expanding on our knowledge and would be continuously having to try to invent them for ourselves.<br />
</p><br />
<br />
<h2>Jaemor Farms:</h2><br />
<p><br />
On November 25th, the Lambert iGEM team went to Jaemor Farms to interview the local farmers and producers of fresh fruits and vegetables. The main objective was to find out how spoiled produce really affected the agricultural industry, such as in amount of profit lost per year due to spoiled peaches. The team toured the farm, interviewed the farm’s owner, and had a great time eating fresh homemade desserts. We came back with some impressive statistics- in 2013, Jaemor Farms produced 700,350 pounds of peaches, 124,949 pounds of strawberries, 115,204 pounds of tomatoes, and 48,000 pounds of apples, among the over 300 different products produced. However, according to global stats, over 50% of fresh produce is lost to spoilage every year across the world. That translates to only half of all the peaches harvested that will make it to the grocery store. With this percentage in mind, the previous numbers cited now seem very small in retrospect. Hunger is a huge problem in less developed countries, and still plagues 1 out of every 6 person in the US. If the LHS iGEM team’s 2014 project is to succeed, it can potentially save world hunger in the long run by preventing food spoilage and increasing the “shelf-life” of fresh produce, which would then go to mean more food available for food aid or distribution to those that really need it.<br />
</p><br />
<br />
<h2>Use of Cells for Chitin</h2><br />
<p><br />
Chitin, which is mostly commonly found in the cell walls of fungi and the shells of crustaceans, is the second most abundant natural long-chain polymer. Due to the many industrial and medical applications of chitin’s deacetylated form, chitosan, the extraction of chitin from chemical and biological sources remains very important, especially to our experiment. Chitin and chitosan are mainly extracted through the demineralization, deproteinization, and other decomposition methods, of crustacean shells and fungi cell wall using acids and bases (mainly hydrochloric acid). The principal problem of this method is that dense acid concentrations cause hydrolysis (the cleavage of chemical bonds due the addition of water) of the polymer, which can potentially change the physical and chemical properties of the outcome, lowering the quality of the final product. This problem leads to more interest in more organic extractions through designer cells. Changing the plasmids of designer cells to express chitosan in the presence of chitin and chitin deacetylase allows for higher quality products, which allows more efficient applications and less acid-base dependency.<br />
</p><br />
<br />
<br />
<h2>How to get Chitosan Out of Cells</h2><br />
<p><br />
Chitosan is a vital part of this project. Chitosan is produced from raw materials that naturally contain chitin. Chitosan’s biological sources are often crustacean shells, specifically shrimp shells, and its assay is greater than or equal to 75 percent (deacetylated). The manufacturing process of shrimp leaves behind a waste shell. This shell is then crushed to remove about 90 percent of the protein present. The crushed shells are washed with salt water and decalcified with a diluted form of hydrochloric acid. The shells are washed once again with recycled salt water and deproteinized with diluted sodium hydroxide. The treated shell produces chitin at this point. The chitin is put through deacetylation and treated with concentrated sodium hydroxide to produce chitosan. <br />
</p><br />
<div><br />
<img id="ImageMap"<br />
src="https://static.igem.org/mediawiki/2014hs/b/b2/2014LambertGAWikiMap.png" <br />
alt="Image Mapping (link to all other pages)"<br />
title="Image Map Test" <br />
border="0" <br />
usemap="#myMap"/><br />
<br />
<map name="myMap"><br />
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<br />
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</div><br />
</body><br />
</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GATeam:Lambert GA2014-06-15T20:27:08Z<p>Kahanpparekh: </p>
<hr />
<div><html><br />
<head><br />
<title><br />
Lambert iGEM HomePage<br />
</title><br />
<style type="text/css"><br />
*{<br />
font-family: Arial;<br />
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background-color: #000000;<br />
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<script type="text/javascript" src="http://code.jquery.com/jquery-2.1.1.js"></script><br />
</head><br />
<body><br />
<h1><br />
Lambert_GA iGEM HomePage<br />
</h1><br />
<p><br />
Lambert high school’s iGem team is working with a chitosan based anti-fungal that stops fungi from feeding off the saccharides on the surface, and inside the fruit, which causes rotting. The chitosan, has proven harder to biologically produce. It is currently produced with chemical intensive process that doesn't always create the correct type of chitosan. Chitosan is a deacetylated form of chitin, a very common organic compound found in cell walls. Chitosan is naturally produced in nature as a food source for many organisms. This is due to the protein chitin deacetylase (CDA) which removes an acetyl group from chitin. Our goals is to extract the CDA gene from the organism Saccharomyces cerevisiae, a very well-known strain of yeast, and express it in E. coli to mass produce the enzyme and create chitosan. Lambert iGem is also planning on creating a BioBrick of the CDA gene for future implications. <br />
</p><br />
<div><br />
<img id="ImageMap"<br />
src="https://static.igem.org/mediawiki/2014hs/b/b2/2014LambertGAWikiMap.png" <br />
alt="Image Mapping (link to all other pages)"<br />
title="Image Map Test" <br />
border="0" <br />
usemap="#myMap"/><br />
<br />
<map name="myMap"><br />
<area shape="rect" <br />
alt="Home" <br />
title="HomePage" <br />
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target="_self" <br />
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alt="Fun" <br />
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target="_self" <br />
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target="_self" <br />
/><br />
<br />
</map><br />
</div><br />
</body><br />
</html></div>Kahanpparekhhttp://2014hs.igem.org/Template:Lambert_GATemplateTemplate:Lambert GATemplate2014-06-15T20:20:54Z<p>Kahanpparekh: Created page with "what is this"</p>
<hr />
<div>what is this</div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GATeam:Lambert GA2014-06-15T20:10:02Z<p>Kahanpparekh: </p>
<hr />
<div><html><br />
The WIKI I created is located at the following link: <br />
<a href="https://2014hs.igem.org/Team:Lambert_GA/WIPHomePage"> https://2014hs.igem.org/Team:Lambert_GA/WIPHomePage </a><br />
<br />
</html><br />
[[Image:Lambert_GA_logo.png|200px|center|frame]]<br />
<br />
[[Image:Lambert_GA_team.png|right|frame|Your team picture]]<br />
<br />
<!--- Team Information Link ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[https://igem.org/Team.cgi?year=2013&division=high_school&team_name=Lambert_GA Official Team Profile]<br />
|}<br />
<br />
=Intro=<br />
Tell us about your team, your school!<br />
<br />
The Lambert High School iGem Team was first started in 2013 by Mrs.Standeven... (Insert rest of information)<br />
<br />
==Science! Link ==<br />
[[https://2014hs.igem.org/Science!]]<br />
<br />
==Methods==<br />
Show us how you spent your days.<br />
<br />
===Methods 1===<br />
<br />
===Methods 2===<br />
<br />
=Results=<br />
What did you achieve over the course of your semester?<br />
<br />
=Future Work=<br />
<br />
=Safety=<br />
- '''All''' team members were trained in appropriate techniques for dealing with cell culture, recombinant DNA, and general lab safety.<br />
<br />
- '''All''' lab procedures were done under the supervision of the instructors.<br />
<br />
- '''All''' experiments with recombinant DNA were approved by Dr. Mark Styczynski from the Georgia Institute of Technology.<br />
<br />
- '''All''' disposal of lab waste was treated in accordance with the MSDS or appropriate sterilization of biohazards.<br />
<br />
=Human Practices=<br />
What impact does/will your project have on the public? <br />
<br />
=Acknowledgements=<br />
<br />
'''Our thanks goes out to:'''<br />
<br />
'''-''' ''Dr. Mark Styczynski''<br />
<br />
'''-''' ''Georgia Institute of Technology and his Team''<br />
<br />
'''-''' ''The NSF grant #1254382''<br />
<br />
'''-''' ''New England Biolabs and Chris Cook''<br />
<br />
'''-''' ''Chik fila John’s Creek''<br />
<br />
'''-''' ''Lambert High School and Principal Dr. Gary Davison''<br />
<br />
'''-''' ''The Ge Family''<br />
<br />
'''-''' ''Jaemor Farms''<br />
<br />
'''-''' ''Northside Hospital Forsyth''<br />
<br />
'''-''' and ''III Below Films''.<br />
<br />
'''The Lambert High School iGem team would've never have gotten this far without all of their help and support.'''<br />
<br />
=Fun!=<br />
What was your favorite team snack?? Have a picture of your team mascot? <br />
<br />
(Insert Video?)<br />
<br />
<forum_subtle /></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GATeam:Lambert GA2014-06-15T20:09:39Z<p>Kahanpparekh: </p>
<hr />
<div><html><br />
Our wiki is located at the following link: <br />
<a href="https://2014hs.igem.org/Team:Lambert_GA/WIPHomePage"> https://2014hs.igem.org/Team:Lambert_GA/WIPHomePage </a><br />
<br />
</html><br />
[[Image:Lambert_GA_logo.png|200px|center|frame]]<br />
<br />
[[Image:Lambert_GA_team.png|right|frame|Your team picture]]<br />
<br />
<!--- Team Information Link ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[https://igem.org/Team.cgi?year=2013&division=high_school&team_name=Lambert_GA Official Team Profile]<br />
|}<br />
<br />
=Intro=<br />
Tell us about your team, your school!<br />
<br />
The Lambert High School iGem Team was first started in 2013 by Mrs.Standeven... (Insert rest of information)<br />
<br />
==Science! Link ==<br />
[[https://2014hs.igem.org/Science!]]<br />
<br />
==Methods==<br />
Show us how you spent your days.<br />
<br />
===Methods 1===<br />
<br />
===Methods 2===<br />
<br />
=Results=<br />
What did you achieve over the course of your semester?<br />
<br />
=Future Work=<br />
<br />
=Safety=<br />
- '''All''' team members were trained in appropriate techniques for dealing with cell culture, recombinant DNA, and general lab safety.<br />
<br />
- '''All''' lab procedures were done under the supervision of the instructors.<br />
<br />
- '''All''' experiments with recombinant DNA were approved by Dr. Mark Styczynski from the Georgia Institute of Technology.<br />
<br />
- '''All''' disposal of lab waste was treated in accordance with the MSDS or appropriate sterilization of biohazards.<br />
<br />
=Human Practices=<br />
What impact does/will your project have on the public? <br />
<br />
=Acknowledgements=<br />
<br />
'''Our thanks goes out to:'''<br />
<br />
'''-''' ''Dr. Mark Styczynski''<br />
<br />
'''-''' ''Georgia Institute of Technology and his Team''<br />
<br />
'''-''' ''The NSF grant #1254382''<br />
<br />
'''-''' ''New England Biolabs and Chris Cook''<br />
<br />
'''-''' ''Chik fila John’s Creek''<br />
<br />
'''-''' ''Lambert High School and Principal Dr. Gary Davison''<br />
<br />
'''-''' ''The Ge Family''<br />
<br />
'''-''' ''Jaemor Farms''<br />
<br />
'''-''' ''Northside Hospital Forsyth''<br />
<br />
'''-''' and ''III Below Films''.<br />
<br />
'''The Lambert High School iGem team would've never have gotten this far without all of their help and support.'''<br />
<br />
=Fun!=<br />
What was your favorite team snack?? Have a picture of your team mascot? <br />
<br />
(Insert Video?)<br />
<br />
<forum_subtle /></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GATeam:Lambert GA2014-06-15T20:08:35Z<p>Kahanpparekh: </p>
<hr />
<div><html><br />
Our wiki is located at the following link: [[https://2014hs.igem.org/Team:Lambert_GA/WIPHomePage]]<br />
<br />
</html><br />
[[Image:Lambert_GA_logo.png|200px|center|frame]]<br />
<br />
[[Image:Lambert_GA_team.png|right|frame|Your team picture]]<br />
<br />
<!--- Team Information Link ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[https://igem.org/Team.cgi?year=2013&division=high_school&team_name=Lambert_GA Official Team Profile]<br />
|}<br />
<br />
=Intro=<br />
Tell us about your team, your school!<br />
<br />
The Lambert High School iGem Team was first started in 2013 by Mrs.Standeven... (Insert rest of information)<br />
<br />
==Science! Link ==<br />
[[https://2014hs.igem.org/Science!]]<br />
<br />
==Methods==<br />
Show us how you spent your days.<br />
<br />
===Methods 1===<br />
<br />
===Methods 2===<br />
<br />
=Results=<br />
What did you achieve over the course of your semester?<br />
<br />
=Future Work=<br />
<br />
=Safety=<br />
- '''All''' team members were trained in appropriate techniques for dealing with cell culture, recombinant DNA, and general lab safety.<br />
<br />
- '''All''' lab procedures were done under the supervision of the instructors.<br />
<br />
- '''All''' experiments with recombinant DNA were approved by Dr. Mark Styczynski from the Georgia Institute of Technology.<br />
<br />
- '''All''' disposal of lab waste was treated in accordance with the MSDS or appropriate sterilization of biohazards.<br />
<br />
=Human Practices=<br />
What impact does/will your project have on the public? <br />
<br />
=Acknowledgements=<br />
<br />
'''Our thanks goes out to:'''<br />
<br />
'''-''' ''Dr. Mark Styczynski''<br />
<br />
'''-''' ''Georgia Institute of Technology and his Team''<br />
<br />
'''-''' ''The NSF grant #1254382''<br />
<br />
'''-''' ''New England Biolabs and Chris Cook''<br />
<br />
'''-''' ''Chik fila John’s Creek''<br />
<br />
'''-''' ''Lambert High School and Principal Dr. Gary Davison''<br />
<br />
'''-''' ''The Ge Family''<br />
<br />
'''-''' ''Jaemor Farms''<br />
<br />
'''-''' ''Northside Hospital Forsyth''<br />
<br />
'''-''' and ''III Below Films''.<br />
<br />
'''The Lambert High School iGem team would've never have gotten this far without all of their help and support.'''<br />
<br />
=Fun!=<br />
What was your favorite team snack?? Have a picture of your team mascot? <br />
<br />
(Insert Video?)<br />
<br />
<forum_subtle /></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/FunTeam:Lambert GA/Fun2014-06-15T20:05:52Z<p>Kahanpparekh: </p>
<hr />
<div><html><br />
<head><br />
<title><br />
Lambert iGEM Fun<br />
</title><br />
<style type="text/css"><br />
*{<br />
font-family: Arial;<br />
background-color: #5BC236;<br />
}<br />
h1{<br />
font-family: "Times New Roman";<br />
color: #000000;<br />
text-align: center;<br />
}<br />
h2{<br />
font-family: Arial;<br />
color: #12260a;<br />
text-align: left;<br />
}<br />
p{<br />
font-family: Verdana;<br />
color: #1B3A10;<br />
padding: 5px 5px 5px 5px;<br />
text-align: justify;<br />
text-indent: 15px;<br />
}<br />
#ImageMap{<br />
display: block;<br />
margin-left: auto;<br />
margin-right: auto;<br />
}<br />
<br />
</style><br />
<script type="text/javascript" src="http://code.jquery.com/jquery-2.1.1.js"></script><br />
</head><br />
<body><br />
<h1>Lambert iGEM Fun</h1><br />
<h2>Video</h2><br />
<p><br />
Mrs. Standeven still has to complete the video. I will embed it as soon as she finishes it.<br />
</p><br />
<br />
<div><br />
<img id="ImageMap"<br />
src="https://static.igem.org/mediawiki/2014hs/b/b2/2014LambertGAWikiMap.png" <br />
alt="Image Mapping (link to all other pages)"<br />
title="Image Map Test" <br />
border="0" <br />
usemap="#myMap"/><br />
<br />
<map name="myMap"><br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/NoteBookTeam:Lambert GA/NoteBook2014-06-15T20:04:12Z<p>Kahanpparekh: </p>
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<h1>Lambert iGEM NoteBook</h1><br />
<p><br />
Jared Cook still has to upload his Notebook.<br />
</p><br />
<br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/FunTeam:Lambert GA/Fun2014-06-15T20:03:58Z<p>Kahanpparekh: Created page with "<html> <head> <title> Lambert iGEM Fun </title> <style type="text/css"> *{ font-family: Arial; background-color: #5BC236; } h1{ ..."</p>
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<h1>Lambert iGEM Fun</h1><br />
<h2>Video</h2><br />
<p><br />
Mrs. Standeven still has to complete the video. We can embed it as soon as they finish it.<br />
</p><br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/HumanPracticesTeam:Lambert GA/HumanPractices2014-06-15T20:03:44Z<p>Kahanpparekh: Created page with "<html> <head> <title> Lambert iGEM Human Practices (Outreach) </title> <style type="text/css"> *{ font-family: Arial; background-color: #421C52; }..."</p>
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<h1>Lambert iGEM Human Practices (Outreach)</h1><br />
<h2>NGF Tutoring:</h2><br />
<p><br />
...<br />
</p><br />
<br />
<h2>Whitlow Elemtary:</h2><br />
<p><br />
As part of iGEM’s goal to expand the ideas of biotechnology in the community, a group of iGEM students participated at the annual STEM Night at Whitlow Elementary. Here a diverse collection of subjects were discussed and iGEM was able to demonstrate common biotechnology applications to young children. The children were amazed and demonstrated curiosity in the synthetic biotechnology field. We demonstrated how a micropipette is used and the miniscule volume that is measured by them. Furthermore, a gel electrophoresis was set up to show some of the unique apparatus we work with. The parents were able to gain an understanding of the objectives of iGEM. In addition, iGEM garnered recognition in the community for a well-developed club that was able to accomplish admirable feats in just a year of its inauguration.<br />
<br />
</p><br />
<div><br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/SafetyTeam:Lambert GA/Safety2014-06-15T20:03:29Z<p>Kahanpparekh: </p>
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<h1>Lambert iGEM Safety</h1><br />
<ul> How We Lab Safely<br />
<li> <b>ALL</b> team members were trained in appropriate techniques for dealing with cell culture, recombinant DNA, and general lab safety.</li><br />
<li> <b>ALL</b> lab procedures were done under the supervision of the instructors.</li><br />
<li> <b>ALL</b> experiments with recombinant DNA were approved by Dr. Mark Styczynski from the Georgia Institute of Technology.</li><br />
<li> <b>ALL</b> disposal of lab waste was treated in accordance with the MSDS or appropriate sterilization of biohazards.</li><br />
</ul><br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/TeamTeam:Lambert GA/Team2014-06-15T20:03:08Z<p>Kahanpparekh: </p>
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<h1>Lambert iGEM Team</h1><br />
<p><br />
This Page Requires Image Uploads. Will Finish Later.<br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/SponsorsTeam:Lambert GA/Sponsors2014-06-15T20:02:47Z<p>Kahanpparekh: </p>
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<h1>Lambert iGEM Sponsors</h1><br />
<h2>Ge Family</h2><br />
<h2><a href="http://www.chick-fil-a.com/johnscreek" target="_blank">John's Creek Chikfila</a></h2><br />
<h2><a href="https://www.neb.com/?gclid=CNzq17vG_L4CFRJk7Aodi3gAuA" target="_blank">New England BioLabs</a></h2><br />
<h2><a href="http://www.nsf.gov/awardsearch/showAward?AWD_ID=1254382" target="_blank">National Science Foundation Grant #1254382</a></h2><br />
<h2><a href="http://chbe.gatech.edu/faculty/styczynski" target="_blank">The Styczynski Lab at Georgia Institue of Technology</a></h2><br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/ProjectTeam:Lambert GA/Project2014-06-15T20:02:30Z<p>Kahanpparekh: </p>
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<h1>Lambert iGEM Project</h1><br />
<h2>Biobricks:</h2><br />
<p><br />
Biobricks are defined as the “…standard for interchangeable parts, developed with a view to building biological systems in living cells” by the iGEM Parts website. Our contribution to CDA would be essential to biobrick because it will allow others to use the part in the future. The biobrick that is created will be mapped out and put into a database which is basically a free dictionary of biobricks. This compilation of biobricks allow others to use them in their experiments, expand on, or restructure. Without a database of biobricks, we would have a hard time expanding on our knowledge and would be continuously having to try to invent them for ourselves.<br />
</p><br />
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<h2>Jaemor Farms:</h2><br />
<p><br />
On November 25th, the Lambert iGEM team went to Jaemor Farms to interview the local farmers and producers of fresh fruits and vegetables. The main objective was to find out how spoiled produce really affected the agricultural industry, such as in amount of profit lost per year due to spoiled peaches. The team toured the farm, interviewed the farm’s owner, and had a great time eating fresh homemade desserts. We came back with some impressive statistics- in 2013, Jaemor Farms produced 700,350 pounds of peaches, 124,949 pounds of strawberries, 115,204 pounds of tomatoes, and 48,000 pounds of apples, among the over 300 different products produced. However, according to global stats, over 50% of fresh produce is lost to spoilage every year across the world. That translates to only half of all the peaches harvested that will make it to the grocery store. With this percentage in mind, the previous numbers cited now seem very small in retrospect. Hunger is a huge problem in less developed countries, and still plagues 1 out of every 6 person in the US. If the LHS iGEM team’s 2014 project is to succeed, it can potentially save world hunger in the long run by preventing food spoilage and increasing the “shelf-life” of fresh produce, which would then go to mean more food available for food aid or distribution to those that really need it.<br />
</p><br />
<br />
<h2>Use of Cells for Chitin</h2><br />
<p><br />
Chitin, which is mostly commonly found in the cell walls of fungi and the shells of crustaceans, is the second most abundant natural long-chain polymer. Due to the many industrial and medical applications of chitin’s deacetylated form, chitosan, the extraction of chitin from chemical and biological sources remains very important, especially to our experiment. Chitin and chitosan are mainly extracted through the demineralization, deproteinization, and other decomposition methods, of crustacean shells and fungi cell wall using acids and bases (mainly hydrochloric acid). The principal problem of this method is that dense acid concentrations cause hydrolysis (the cleavage of chemical bonds due the addition of water) of the polymer, which can potentially change the physical and chemical properties of the outcome, lowering the quality of the final product. This problem leads to more interest in more organic extractions through designer cells. Changing the plasmids of designer cells to express chitosan in the presence of chitin and chitin deacetylase allows for higher quality products, which allows more efficient applications and less acid-base dependency.<br />
</p><br />
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<br />
<h2>How to get Chitosan Out of Cells</h2><br />
<p><br />
Chitosan is a vital part of this project. Chitosan is produced from raw materials that naturally contain chitin. Chitosan’s biological sources are often crustacean shells, specifically shrimp shells, and its assay is greater than or equal to 75 percent (deacetylated). The manufacturing process of shrimp leaves behind a waste shell. This shell is then crushed to remove about 90 percent of the protein present. The crushed shells are washed with salt water and decalcified with a diluted form of hydrochloric acid. The shells are washed once again with recycled salt water and deproteinized with diluted sodium hydroxide. The treated shell produces chitin at this point. The chitin is put through deacetylation and treated with concentrated sodium hydroxide to produce chitosan. <br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/WIPHomePageTeam:Lambert GA/WIPHomePage2014-06-15T20:02:12Z<p>Kahanpparekh: </p>
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Lambert_GA iGEM HomePage<br />
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<p><br />
Lambert high school’s iGem team is working with a chitosan based anti-fungal that stops fungi from feeding off the saccharides on the surface, and inside the fruit, which causes rotting. The chitosan, has proven harder to biologically produce. It is currently produced with chemical intensive process that doesn't always create the correct type of chitosan. Chitosan is a deacetylated form of chitin, a very common organic compound found in cell walls. Chitosan is naturally produced in nature as a food source for many organisms. This is due to the protein chitin deacetylase (CDA) which removes an acetyl group from chitin. Our goals is to extract the CDA gene from the organism Saccharomyces cerevisiae, a very well-known strain of yeast, and express it in E. coli to mass produce the enzyme and create chitosan. Lambert iGem is also planning on creating a BioBrick of the CDA gene for future implications. <br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/SafetyTeam:Lambert GA/Safety2014-06-15T19:04:38Z<p>Kahanpparekh: Created page with "abcd"</p>
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<div>abcd</div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/TeamTeam:Lambert GA/Team2014-06-15T19:04:20Z<p>Kahanpparekh: </p>
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<div>This Page Requires Image Uploads. Will Finish Later.</div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/SponsorsTeam:Lambert GA/Sponsors2014-06-15T19:00:12Z<p>Kahanpparekh: </p>
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<h1>Ge Family</h1><br />
<h1><a href="http://www.chick-fil-a.com/johnscreek" target="_blank">John's Creek Chikfila</a></h1><br />
<h1><a href="https://www.neb.com/?gclid=CNzq17vG_L4CFRJk7Aodi3gAuA" target="_blank">New England BioLabs</a></h1><br />
<h1><a href="http://www.nsf.gov/awardsearch/showAward?AWD_ID=1254382" target="_blank">National Science Foundation Grant #1254382</a></h1><br />
<h1><a href="http://chbe.gatech.edu/faculty/styczynski" target="_blank">The Styczynski Lab at Georgia Institue of Technology</a></h1><br />
<div><br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/ProjectTeam:Lambert GA/Project2014-06-15T18:45:36Z<p>Kahanpparekh: </p>
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Lambert iGEM Project<br />
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<h1>Biobricks:</h1><br />
<p><br />
Biobricks are defined as the “…standard for interchangeable parts, developed with a view to building biological systems in living cells” by the iGEM Parts website. Our contribution to CDA would be essential to biobrick because it will allow others to use the part in the future. The biobrick that is created will be mapped out and put into a database which is basically a free dictionary of biobricks. This compilation of biobricks allow others to use them in their experiments, expand on, or restructure. Without a database of biobricks, we would have a hard time expanding on our knowledge and would be continuously having to try to invent them for ourselves.<br />
</p><br />
<br />
<h1>Jaemor Farms:</h1><br />
<p><br />
On November 25th, the Lambert iGEM team went to Jaemor Farms to interview the local farmers and producers of fresh fruits and vegetables. The main objective was to find out how spoiled produce really affected the agricultural industry, such as in amount of profit lost per year due to spoiled peaches. The team toured the farm, interviewed the farm’s owner, and had a great time eating fresh homemade desserts. We came back with some impressive statistics- in 2013, Jaemor Farms produced 700,350 pounds of peaches, 124,949 pounds of strawberries, 115,204 pounds of tomatoes, and 48,000 pounds of apples, among the over 300 different products produced. However, according to global stats, over 50% of fresh produce is lost to spoilage every year across the world. That translates to only half of all the peaches harvested that will make it to the grocery store. With this percentage in mind, the previous numbers cited now seem very small in retrospect. Hunger is a huge problem in less developed countries, and still plagues 1 out of every 6 person in the US. If the LHS iGEM team’s 2014 project is to succeed, it can potentially save world hunger in the long run by preventing food spoilage and increasing the “shelf-life” of fresh produce, which would then go to mean more food available for food aid or distribution to those that really need it.<br />
</p><br />
<br />
<h1>Use of Cells for Chitin</h1><br />
<p><br />
Chitin, which is mostly commonly found in the cell walls of fungi and the shells of crustaceans, is the second most abundant natural long-chain polymer. Due to the many industrial and medical applications of chitin’s deacetylated form, chitosan, the extraction of chitin from chemical and biological sources remains very important, especially to our experiment. Chitin and chitosan are mainly extracted through the demineralization, deproteinization, and other decomposition methods, of crustacean shells and fungi cell wall using acids and bases (mainly hydrochloric acid). The principal problem of this method is that dense acid concentrations cause hydrolysis (the cleavage of chemical bonds due the addition of water) of the polymer, which can potentially change the physical and chemical properties of the outcome, lowering the quality of the final product. This problem leads to more interest in more organic extractions through designer cells. Changing the plasmids of designer cells to express chitosan in the presence of chitin and chitin deacetylase allows for higher quality products, which allows more efficient applications and less acid-base dependency.<br />
</p><br />
<br />
<br />
<h1>How to get Chitosan Out of Cells</h1><br />
<p><br />
Chitosan is a vital part of this project. Chitosan is produced from raw materials that naturally contain chitin. Chitosan’s biological sources are often crustacean shells, specifically shrimp shells, and its assay is greater than or equal to 75 percent (deacetylated). The manufacturing process of shrimp leaves behind a waste shell. This shell is then crushed to remove about 90 percent of the protein present. The crushed shells are washed with salt water and decalcified with a diluted form of hydrochloric acid. The shells are washed once again with recycled salt water and deproteinized with diluted sodium hydroxide. The treated shell produces chitin at this point. The chitin is put through deacetylation and treated with concentrated sodium hydroxide to produce chitosan. <br />
</p><br />
<div><br />
<img id="ImageMap"<br />
src="https://static.igem.org/mediawiki/2014hs/b/b2/2014LambertGAWikiMap.png" <br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/ProjectTeam:Lambert GA/Project2014-06-15T18:43:07Z<p>Kahanpparekh: </p>
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Lambert iGEM Project<br />
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<h1>Biobricks:</h1><br />
<p><br />
Biobricks are defined as the “…standard for interchangeable parts, developed with a view to building biological systems in living cells” by the iGEM Parts website. Our contribution to CDA would be essential to biobrick because it will allow others to use the part in the future. The biobrick that is created will be mapped out and put into a database which is basically a free dictionary of biobricks. This compilation of biobricks allow others to use them in their experiments, expand on, or restructure. Without a database of biobricks, we would have a hard time expanding on our knowledge and would be continuously having to try to invent them for ourselves.<br />
</p><br />
<br />
<h1>Jaemor Farms:</h1><br />
<p><br />
On November 25th, the Lambert iGEM team went to Jaemor Farms to interview the local farmers and producers of fresh fruits and vegetables. The main objective was to find out how spoiled produce really affected the agricultural industry, such as in amount of profit lost per year due to spoiled peaches. The team toured the farm, interviewed the farm’s owner, and had a great time eating fresh homemade desserts. We came back with some impressive statistics- in 2013, Jaemor Farms produced 700,350 pounds of peaches, 124,949 pounds of strawberries, 115,204 pounds of tomatoes, and 48,000 pounds of apples, among the over 300 different products produced. However, according to global stats, over 50% of fresh produce is lost to spoilage every year across the world. That translates to only half of all the peaches harvested that will make it to the grocery store. With this percentage in mind, the previous numbers cited now seem very small in retrospect. Hunger is a huge problem in less developed countries, and still plagues 1 out of every 6 person in the US. If the LHS iGEM team’s 2014 project is to succeed, it can potentially save world hunger in the long run by preventing food spoilage and increasing the “shelf-life” of fresh produce, which would then go to mean more food available for food aid or distribution to those that really need it.<br />
</p><br />
<br />
<h1>Use of Cells for Chitin</h1><br />
<p><br />
Chitin, which is mostly commonly found in the cell walls of fungi and the shells of crustaceans, is the second most abundant natural long-chain polymer. Due to the many industrial and medical applications of chitin’s deacetylated form, chitosan, the extraction of chitin from chemical and biological sources remains very important, especially to our experiment. Chitin and chitosan are mainly extracted through the demineralization, deproteinization, and other decomposition methods, of crustacean shells and fungi cell wall using acids and bases (mainly hydrochloric acid). The principal problem of this method is that dense acid concentrations cause hydrolysis (the cleavage of chemical bonds due the addition of water) of the polymer, which can potentially change the physical and chemical properties of the outcome, lowering the quality of the final product. This problem leads to more interest in more organic extractions through designer cells. Changing the plasmids of designer cells to express chitosan in the presence of chitin and chitin deacetylase allows for higher quality products, which allows more efficient applications and less acid-base dependency.<br />
</p><br />
<br />
<br />
<h1>How to get Chitosan Out of Cells</h1><br />
<p><br />
Chitosan is a vital part of this project. Chitosan is produced from raw materials that naturally contain chitin. Chitosan’s biological sources are often crustacean shells, specifically shrimp shells, and its assay is greater than or equal to 75 percent (deacetylated). The manufacturing process of shrimp leaves behind a waste shell. This shell is then crushed to remove about 90 percent of the protein present. The crushed shells are washed with salt water and decalcified with a diluted form of hydrochloric acid. The shells are washed once again with recycled salt water and deproteinized with diluted sodium hydroxide. The treated shell produces chitin at this point. The chitin is put through deacetylation and treated with concentrated sodium hydroxide to produce chitosan. <br />
</p><br />
<div><br />
<img id="ImageMap"<br />
src="https://static.igem.org/mediawiki/2014hs/b/b2/2014LambertGAWikiMap.png" <br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/ProjectTeam:Lambert GA/Project2014-06-15T18:38:51Z<p>Kahanpparekh: </p>
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Lambert iGEM Project<br />
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<h2>Biobricks:</h2><br />
<p><br />
Biobricks are defined as the “…standard for interchangeable parts, developed with a view to building biological systems in living cells” by the iGEM Parts website. Our contribution to CDA would be essential to biobrick because it will allow others to use the part in the future. The biobrick that is created will be mapped out and put into a database which is basically a free dictionary of biobricks. This compilation of biobricks allow others to use them in their experiments, expand on, or restructure. Without a database of biobricks, we would have a hard time expanding on our knowledge and would be continuously having to try to invent them for ourselves.<br />
</p><br />
<br />
<h2>Jaemor Farms:</h2><br />
<p><br />
On November 25th, the Lambert iGEM team went to Jaemor Farms to interview the local farmers and producers of fresh fruits and vegetables. The main objective was to find out how spoiled produce really affected the agricultural industry, such as in amount of profit lost per year due to spoiled peaches. The team toured the farm, interviewed the farm’s owner, and had a great time eating fresh homemade desserts. We came back with some impressive statistics- in 2013, Jaemor Farms produced 700,350 pounds of peaches, 124,949 pounds of strawberries, 115,204 pounds of tomatoes, and 48,000 pounds of apples, among the over 300 different products produced. However, according to global stats, over 50% of fresh produce is lost to spoilage every year across the world. That translates to only half of all the peaches harvested that will make it to the grocery store. With this percentage in mind, the previous numbers cited now seem very small in retrospect. Hunger is a huge problem in less developed countries, and still plagues 1 out of every 6 person in the US. If the LHS iGEM team’s 2014 project is to succeed, it can potentially save world hunger in the long run by preventing food spoilage and increasing the “shelf-life” of fresh produce, which would then go to mean more food available for food aid or distribution to those that really need it.<br />
</p><br />
<br />
<h2>Use of Cells for Chitin</h2><br />
<p><br />
Chitin, which is mostly commonly found in the cell walls of fungi and the shells of crustaceans, is the second most abundant natural long-chain polymer. Due to the many industrial and medical applications of chitin’s deacetylated form, chitosan, the extraction of chitin from chemical and biological sources remains very important, especially to our experiment. Chitin and chitosan are mainly extracted through the demineralization, deproteinization, and other decomposition methods, of crustacean shells and fungi cell wall using acids and bases (mainly hydrochloric acid). The principal problem of this method is that dense acid concentrations cause hydrolysis (the cleavage of chemical bonds due the addition of water) of the polymer, which can potentially change the physical and chemical properties of the outcome, lowering the quality of the final product. This problem leads to more interest in more organic extractions through designer cells. Changing the plasmids of designer cells to express chitosan in the presence of chitin and chitin deacetylase allows for higher quality products, which allows more efficient applications and less acid-base dependency.<br />
</p><br />
<br />
<br />
<h2>How to get Chitosan Out of Cells</h2><br />
<p><br />
Chitosan is a vital part of this project. Chitosan is produced from raw materials that naturally contain chitin. Chitosan’s biological sources are often crustacean shells, specifically shrimp shells, and its assay is greater than or equal to 75 percent (deacetylated). The manufacturing process of shrimp leaves behind a waste shell. This shell is then crushed to remove about 90 percent of the protein present. The crushed shells are washed with salt water and decalcified with a diluted form of hydrochloric acid. The shells are washed once again with recycled salt water and deproteinized with diluted sodium hydroxide. The treated shell produces chitin at this point. The chitin is put through deacetylation and treated with concentrated sodium hydroxide to produce chitosan. <br />
</p><br />
<div><br />
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src="https://static.igem.org/mediawiki/2014hs/b/b2/2014LambertGAWikiMap.png" <br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/File:2014LambertGAWikiMap.pngFile:2014LambertGAWikiMap.png2014-06-15T18:21:11Z<p>Kahanpparekh: uploaded a new version of &quot;File:2014LambertGAWikiMap.png&quot;</p>
<hr />
<div></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/WIPHomePageTeam:Lambert GA/WIPHomePage2014-06-15T18:14:43Z<p>Kahanpparekh: </p>
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Lambert_GA iGEM HomePage<br />
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<p><br />
Lambert high school’s iGem team is working with a chitosan based anti-fungal that stops fungi from feeding off the saccharides on the surface, and inside the fruit, which causes rotting. The chitosan, has proven harder to biologically produce. It is currently produced with chemical intensive process that doesn't always create the correct type of chitosan. Chitosan is a deacetylated form of chitin, a very common organic compound found in cell walls. Chitosan is naturally produced in nature as a food source for many organisms. This is due to the protein chitin deacetylase (CDA) which removes an acetyl group from chitin. Our goals is to extract the CDA gene from the organism Saccharomyces cerevisiae, a very well-known strain of yeast, and express it in E. coli to mass produce the enzyme and create chitosan. Lambert iGem is also planning on creating a BioBrick of the CDA gene for future implications. <br />
</p><br />
<p><br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/File:2014LambertGAWikiMap.pngFile:2014LambertGAWikiMap.png2014-06-15T18:13:16Z<p>Kahanpparekh: </p>
<hr />
<div></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/WIPHomePageTeam:Lambert GA/WIPHomePage2014-06-15T17:39:10Z<p>Kahanpparekh: </p>
<hr />
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<title><br />
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</head><br />
<body><br />
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Lambert_GA iGEM HomePage<br />
</h1><br />
<p><br />
Lambert high school’s iGem team is working with a chitosan based anti-fungal that stops fungi from feeding off the saccharides on the surface, and inside the fruit, which causes rotting. The chitosan, has proven harder to biologically produce. It is currently produced with chemical intensive process that doesn't always create the correct type of chitosan. Chitosan is a deacetylated form of chitin, a very common organic compound found in cell walls. Chitosan is naturally produced in nature as a food source for many organisms. This is due to the protein chitin deacetylase (CDA) which removes an acetyl group from chitin. Our goals is to extract the CDA gene from the organism Saccharomyces cerevisiae, a very well-known strain of yeast, and express it in E. coli to mass produce the enzyme and create chitosan. Lambert iGem is also planning on creating a BioBrick of the CDA gene for future implications. <br />
</p><br />
<p><br />
<img id="ImageMap"<br />
src="https://static.igem.org/mediawiki/2014hs/a/a4/Imagemaptest.png" <br />
alt="Image Mapping (link to all other pages)"<br />
title="Image Map Test" <br />
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usemap="#myMap"/><br />
<br />
<map name="myMap"><br />
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<br />
</map><br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/WIPHomePageTeam:Lambert GA/WIPHomePage2014-06-15T17:37:51Z<p>Kahanpparekh: </p>
<hr />
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</head><br />
<body><br />
<h1><br />
Lambert_GA iGEM HomePage<br />
</h1><br />
<p><br />
Lambert high school’s iGem team is working with a chitosan based anti-fungal that stops fungi from feeding off the saccharides on the surface, and inside the fruit, which causes rotting. The chitosan, has proven harder to biologically produce. It is currently produced with chemical intensive process that doesn't always create the correct type of chitosan. Chitosan is a deacetylated form of chitin, a very common organic compound found in cell walls. Chitosan is naturally produced in nature as a food source for many organisms. This is due to the protein chitin deacetylase (CDA) which removes an acetyl group from chitin. Our goals is to extract the CDA gene from the organism Saccharomyces cerevisiae, a very well-known strain of yeast, and express it in E. coli to mass produce the enzyme and create chitosan. Lambert iGem is also planning on creating a BioBrick of the CDA gene for future implications. <br />
</p><br />
<p><br />
<img id="ImageMap"<br />
src="https://static.igem.org/mediawiki/2014hs/a/a4/Imagemaptest.png" <br />
alt="Image Mapping (link to all other pages)"<br />
title="Image Map Test" <br />
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<br />
</map><br />
</p><br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/WIPHomePageTeam:Lambert GA/WIPHomePage2014-06-15T17:31:06Z<p>Kahanpparekh: </p>
<hr />
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Lambert iGEM HomePage<br />
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<body><br />
<h1><br />
Lambert_GA iGEM HomePage<br />
</h1><br />
<p><br />
Lambert high school’s iGem team is working with a chitosan based anti-fungal that stops fungi from feeding off the saccharides on the surface, and inside the fruit, which causes rotting. The chitosan, has proven harder to biologically produce. It is currently produced with chemical intensive process that doesn't always create the correct type of chitosan. Chitosan is a deacetylated form of chitin, a very common organic compound found in cell walls. Chitosan is naturally produced in nature as a food source for many organisms. This is due to the protein chitin deacetylase (CDA) which removes an acetyl group from chitin. Our goals is to extract the CDA gene from the organism Saccharomyces cerevisiae, a very well-known strain of yeast, and express it in E. coli to mass produce the enzyme and create chitosan. Lambert iGem is also planning on creating a BioBrick of the CDA gene for future implications. <br />
</p><br />
<p><br />
<img src="https://static.igem.org/mediawiki/2014hs/a/a4/Imagemaptest.png" title="Image Map Test" <br />
border="0" usemap="#myMap"/><br />
<br />
<map name="myMap"><br />
<area shape="rect" <br />
coords="66,52,335,88"<br />
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<br />
</map><br />
</p><br />
</body><br />
</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/WIPHomePage2Team:Lambert GA/WIPHomePage22014-06-15T17:26:44Z<p>Kahanpparekh: </p>
<hr />
<div><html><br />
<head><br />
<title><br />
Lambert iGEM HomePage<br />
</title><br />
<style type="text/css"><br />
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<body><br />
<h1><br />
Lambert_GA iGEM HomePage<br />
</h1><br />
<p><br />
Lambert high school’s iGem team is working with a chitosan based anti-fungal that stops fungi from feeding off the saccharides on the surface, and inside the fruit, which causes rotting. The chitosan, has proven harder to biologically produce. It is currently produced with chemical intensive process that doesn't always create the correct type of chitosan. Chitosan is a deacetylated form of chitin, a very common organic compound found in cell walls. Chitosan is naturally produced in nature as a food source for many organisms. This is due to the protein chitin deacetylase (CDA) which removes an acetyl group from chitin. Our goals is to extract the CDA gene from the organism Saccharomyces cerevisiae, a very well-known strain of yeast, and express it in E. coli to mass produce the enzyme and create chitosan. Lambert iGem is also planning on creating a BioBrick of the CDA gene for future implications. <br />
</p><br />
</body><br />
</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/WIPHomePage2Team:Lambert GA/WIPHomePage22014-06-15T17:26:28Z<p>Kahanpparekh: </p>
<hr />
<div><html><br />
<head><br />
<title><br />
Lambert iGEM HomePage<br />
</title><br />
<style type="text/css"><br />
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<body><br />
<h1><br />
Lambert_GA iGEM HomePage<br />
</h1><br />
<p><br />
Lambert high school’s iGem team is working with a chitosan based anti-fungal that stops fungi from feeding off the saccharides on the surface, and inside the fruit, which causes rotting. The chitosan, has proven harder to biologically produce. It is currently produced with chemical intensive process that doesn't always create the correct type of chitosan. Chitosan is a deacetylated form of chitin, a very common organic compound found in cell walls. Chitosan is naturally produced in nature as a food source for many organisms. This is due to the protein chitin deacetylase (CDA) which removes an acetyl group from chitin. Our goals is to extract the CDA gene from the organism Saccharomyces cerevisiae, a very well-known strain of yeast, and express it in E. coli to mass produce the enzyme and create chitosan. Lambert iGem is also planning on creating a BioBrick of the CDA gene for future implications. <br />
</p><br />
</body><br />
</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/WIPHomePage2Team:Lambert GA/WIPHomePage22014-06-15T17:25:10Z<p>Kahanpparekh: </p>
<hr />
<div><html><br />
<head><br />
<title><br />
Lambert iGEM HomePage<br />
</title><br />
<style type="text/css"><br />
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<body><br />
<h1><br />
Lambert_GA iGEM HomePage<br />
</h1><br />
<p><br />
Lambert high school’s iGem team is working with a chitosan based anti-fungal that stops fungi from feeding off the saccharides on the surface, and inside the fruit, which causes rotting. The chitosan, has proven harder to biologically produce. It is currently produced with chemical intensive process that doesn't always create the correct type of chitosan. Chitosan is a deacetylated form of chitin, a very common organic compound found in cell walls. Chitosan is naturally produced in nature as a food source for many organisms. This is due to the protein chitin deacetylase (CDA) which removes an acetyl group from chitin. Our goals is to extract the CDA gene from the organism Saccharomyces cerevisiae, a very well-known strain of yeast, and express it in E. coli to mass produce the enzyme and create chitosan. Lambert iGem is also planning on creating a BioBrick of the CDA gene for future implications. <br />
</p><br />
</body><br />
</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/WIPHomePage2Team:Lambert GA/WIPHomePage22014-06-15T17:19:13Z<p>Kahanpparekh: </p>
<hr />
<div><html><br />
<head><br />
<title><br />
Lambert iGEM HomePage<br />
</title><br />
<style type="text/css"><br />
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<body><br />
<p><br />
Lambert high school’s iGem team is working with a chitosan based anti-fungal that stops fungi from feeding off the saccharides on the surface, and inside the fruit, which causes rotting. The chitosan, has proven harder to biologically produce. It is currently produced with chemical intensive process that doesn't always create the correct type of chitosan. Chitosan is a deacetylated form of chitin, a very common organic compound found in cell walls. Chitosan is naturally produced in nature as a food source for many organisms. This is due to the protein chitin deacetylase (CDA) which removes an acetyl group from chitin. Our goals is to extract the CDA gene from the organism Saccharomyces cerevisiae, a very well-known strain of yeast, and express it in E. coli to mass produce the enzyme and create chitosan. Lambert iGem is also planning on creating a BioBrick of the CDA gene for future implications. <br />
</p><br />
</body><br />
</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/WIPHomePage2Team:Lambert GA/WIPHomePage22014-06-15T17:17:24Z<p>Kahanpparekh: </p>
<hr />
<div><html><br />
<head><br />
<title><br />
Lambert iGEM HomePage<br />
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<p><br />
Lambert high school’s iGem team is working with a chitosan based anti-fungal that stops fungi from feeding off the saccharides on the surface, and inside the fruit, which causes rotting. The chitosan, has proven harder to biologically produce. It is currently produced with chemical intensive process that doesn't always create the correct type of chitosan. Chitosan is a deacetylated form of chitin, a very common organic compound found in cell walls. Chitosan is naturally produced in nature as a food source for many organisms. This is due to the protein chitin deacetylase (CDA) which removes an acetyl group from chitin. Our goals is to extract the CDA gene from the organism Saccharomyces cerevisiae, a very well-known strain of yeast, and express it in E. coli to mass produce the enzyme and create chitosan. Lambert iGem is also planning on creating a BioBrick of the CDA gene for future implications. <br />
</p><br />
</body><br />
</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/WIPHomePage2Team:Lambert GA/WIPHomePage22014-06-15T17:17:08Z<p>Kahanpparekh: </p>
<hr />
<div><html><br />
<head><br />
<title><br />
Lambert iGEM HomePage<br />
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<style type="text/css"><br />
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<p><br />
Lambert high school’s iGem team is working with a chitosan based anti-fungal that stops fungi from feeding off the saccharides on the surface, and inside the fruit, which causes rotting. The chitosan, has proven harder to biologically produce. It is currently produced with chemical intensive process that doesn't always create the correct type of chitosan. Chitosan is a deacetylated form of chitin, a very common organic compound found in cell walls. Chitosan is naturally produced in nature as a food source for many organisms. This is due to the protein chitin deacetylase (CDA) which removes an acetyl group from chitin. Our goals is to extract the CDA gene from the organism Saccharomyces cerevisiae, a very well-known strain of yeast, and express it in E. coli to mass produce the enzyme and create chitosan. Lambert iGem is also planning on creating a BioBrick of the CDA gene for future implications. <br />
</p><br />
</body><br />
</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/WIPHomePage2Team:Lambert GA/WIPHomePage22014-06-15T17:16:36Z<p>Kahanpparekh: Created page with "<html> <head> <title> Lambert iGEM HomePage </title> <style type="text/css"> *{ font-family: Arial; background-color: #666666; } p{ font-family: Ve..."</p>
<hr />
<div><html><br />
<head><br />
<title><br />
Lambert iGEM HomePage<br />
</title><br />
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*{<br />
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p{<br />
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}<br />
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</head><br />
<body><br />
<p><br />
Lambert high school’s iGem team is working with a chitosan based anti-fungal that stops fungi from feeding off the saccharides on the surface, and inside the fruit, which causes rotting. The chitosan, has proven harder to biologically produce. It is currently produced with chemical intensive process that doesn't always create the correct type of chitosan. Chitosan is a deacetylated form of chitin, a very common organic compound found in cell walls. Chitosan is naturally produced in nature as a food source for many organisms. This is due to the protein chitin deacetylase (CDA) which removes an acetyl group from chitin. Our goals is to extract the CDA gene from the organism Saccharomyces cerevisiae, a very well-known strain of yeast, and express it in E. coli to mass produce the enzyme and create chitosan. Lambert iGem is also planning on creating a BioBrick of the CDA gene for future implications. <br />
</p><br />
</body><br />
</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/ProjectTeam:Lambert GA/Project2014-06-14T15:07:55Z<p>Kahanpparekh: </p>
<hr />
<div>Biobricks:<br />
<br />
Biobricks are defined as the “…standard for interchangeable parts, developed with a view to building biological systems in living cells” by the iGEM Parts website. Our contribution to CDA would be essential to biobrick because it will allow others to use the part in the future. The biobrick that is created will be mapped out and put into a database which is basically a free dictionary of biobricks. This compilation of biobricks allow others to use them in their experiments, expand on, or restructure. Without a database of biobricks, we would have a hard time expanding on our knowledge and would be continuously having to try to invent them for ourselves.<br />
<br />
Jaemor Farms:<br />
<br />
On November 25th, the Lambert iGEM team went to Jaemor Farms to interview the local farmers and producers of fresh fruits and vegetables. The main objective was to find out how spoiled produce really affected the agricultural industry, such as in amount of profit lost per year due to spoiled peaches. The team toured the farm, interviewed the farm’s owner, and had a great time eating fresh homemade desserts. We came back with some impressive statistics- in 2013, Jaemor Farms produced 700,350 pounds of peaches, 124,949 pounds of strawberries, 115,204 pounds of tomatoes, and 48,000 pounds of apples, among the over 300 different products produced. However, according to global stats, over 50% of fresh produce is lost to spoilage every year across the world. That translates to only half of all the peaches harvested that will make it to the grocery store. With this percentage in mind, the previous numbers cited now seem very small in retrospect. Hunger is a huge problem in less developed countries, and still plagues 1 out of every 6 person in the US. If the LHS iGEM team’s 2014 project is to succeed, it can potentially save world hunger in the long run by preventing food spoilage and increasing the “shelf-life” of fresh produce, which would then go to mean more food available for food aid or distribution to those that really need it.<br />
<br />
Use of Cells for Chitin<br />
<br />
Chitin, which is mostly commonly found in the cell walls of fungi and the shells of crustaceans, is the second most abundant natural long-chain polymer. Due to the many industrial and medical applications of chitin’s deacetylated form, chitosan, the extraction of chitin from chemical and biological sources remains very important, especially to our experiment.<br />
Chitin and chitosan are mainly extracted through the demineralization, deproteinization, and other decomposition methods, of crustacean shells and fungi cell wall using acids and bases (mainly hydrochloric acid). The principal problem of this method is that dense acid concentrations cause hydrolysis (the cleavage of chemical bonds due the addition of water) of the polymer, which can potentially change the physical and chemical properties of the outcome, lowering the quality of the final product. This problem leads to more interest in more organic extractions through designer cells.<br />
Changing the plasmids of designer cells to express chitosan in the presence of chitin and chitin deacetylase allows for higher quality products, which allows more efficient applications and less acid-base dependency.<br />
<br />
<br />
<br />
How to get Chitosan Out of Cells<br />
<br />
Chitosan is a vital part of this project. Chitosan is produced from raw materials that naturally contain chitin. Chitosan’s biological sources are often crustacean shells, specifically shrimp shells, and its assay is greater than or equal to 75 percent (deacetylated). The manufacturing process of shrimp leaves behind a waste shell. This shell is then crushed to remove about 90 percent of the protein present. The crushed shells are washed with salt water and decalcified with a diluted form of hydrochloric acid. The shells are washed once again with recycled salt water and deproteinized with diluted sodium hydroxide. The treated shell produces chitin at this point. The chitin is put through deacetylation and treated with concentrated sodium hydroxide to produce chitosan.</div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GATeam:Lambert GA2014-06-14T15:05:34Z<p>Kahanpparekh: </p>
<hr />
<div><html><br />
<div id="menu"><br />
<ul><br />
<li class="first"><a href="https://2014hs.igem.org/Team:Lambert_GA" accesskey="1" title="">Intro</a></li><br />
<li><a href="https://2014hs.igem.org/Team:Lambert_GA/Results" accesskey="3" title="">Results</a></li><br />
<li><a href="https://2014hs.igem.org/Team:Lambert_GA/Future" accesskey="2" title="">Future Work</a></li><br />
<li><a href="https://2014hs.igem.org/Team:Lambert_GA/Safety" accesskey="4" title="">Safety</a></li><br />
<li><a href="https://2014hs.igem.org/Team:Lambert_GA/HumanPractices" accesskey="5" title="">Human Practices</a></li><br />
<li><a href="https://2014hs.igem.org/Team:Lambert_GA/Acknowledgements" accesskey="8" title="">Acknowledgements</a></li><br />
<li><a href="https://2014hs.igem.org/Team:Lambert_GA/Fun" accesskey="5" title="">Fun!</a></li><br />
<br />
</ul><br />
<br />
https://2014hs.igem.org/Team:Lambert_GA/WIPHomePage<br />
<br />
</html><br />
[[Image:Lambert_GA_logo.png|200px|center|frame]]<br />
<br />
[[Image:Lambert_GA_team.png|right|frame|Your team picture]]<br />
<br />
<!--- Team Information Link ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[https://igem.org/Team.cgi?year=2013&division=high_school&team_name=Lambert_GA Official Team Profile]<br />
|}<br />
<br />
=Intro=<br />
Tell us about your team, your school!<br />
<br />
The Lambert High School iGem Team was first started in 2013 by Mrs.Standeven... (Insert rest of information)<br />
<br />
==Science! Link ==<br />
[[https://2014hs.igem.org/Science!]]<br />
<br />
==Methods==<br />
Show us how you spent your days.<br />
<br />
===Methods 1===<br />
<br />
===Methods 2===<br />
<br />
=Results=<br />
What did you achieve over the course of your semester?<br />
<br />
=Future Work=<br />
<br />
=Safety=<br />
- '''All''' team members were trained in appropriate techniques for dealing with cell culture, recombinant DNA, and general lab safety.<br />
<br />
- '''All''' lab procedures were done under the supervision of the instructors.<br />
<br />
- '''All''' experiments with recombinant DNA were approved by Dr. Mark Styczynski from the Georgia Institute of Technology.<br />
<br />
- '''All''' disposal of lab waste was treated in accordance with the MSDS or appropriate sterilization of biohazards.<br />
<br />
=Human Practices=<br />
What impact does/will your project have on the public? <br />
<br />
=Acknowledgements=<br />
<br />
'''Our thanks goes out to:'''<br />
<br />
'''-''' ''Dr. Mark Styczynski''<br />
<br />
'''-''' ''Georgia Institute of Technology and his Team''<br />
<br />
'''-''' ''The NSF grant #1254382''<br />
<br />
'''-''' ''New England Biolabs and Chris Cook''<br />
<br />
'''-''' ''Chik fila John’s Creek''<br />
<br />
'''-''' ''Lambert High School and Principal Dr. Gary Davison''<br />
<br />
'''-''' ''The Ge Family''<br />
<br />
'''-''' ''Jaemor Farms''<br />
<br />
'''-''' ''Northside Hospital Forsyth''<br />
<br />
'''-''' and ''III Below Films''.<br />
<br />
'''The Lambert High School iGem team would've never have gotten this far without all of their help and support.'''<br />
<br />
=Fun!=<br />
What was your favorite team snack?? Have a picture of your team mascot? <br />
<br />
(Insert Video?)<br />
<br />
<forum_subtle /></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GATeam:Lambert GA2014-06-14T15:04:46Z<p>Kahanpparekh: </p>
<hr />
<div><html><br />
<div id="menu"><br />
<ul><br />
<li class="first"><a href="https://2014hs.igem.org/Team:Lambert_GA" accesskey="1" title="">Intro</a></li><br />
<li><a href="https://2014hs.igem.org/Team:Lambert_GA/Results" accesskey="3" title="">Results</a></li><br />
<li><a href="https://2014hs.igem.org/Team:Lambert_GA/Future" accesskey="2" title="">Future Work</a></li><br />
<li><a href="https://2014hs.igem.org/Team:Lambert_GA/Safety" accesskey="4" title="">Safety</a></li><br />
<li><a href="https://2014hs.igem.org/Team:Lambert_GA/HumanPractices" accesskey="5" title="">Human Practices</a></li><br />
<li><a href="https://2014hs.igem.org/Team:Lambert_GA/Acknowledgements" accesskey="8" title="">Acknowledgements</a></li><br />
<li><a href="https://2014hs.igem.org/Team:Lambert_GA/Fun" accesskey="5" title="">Fun!</a></li><br />
<br />
</ul><br />
<br />
</html><br />
[[Image:Lambert_GA_logo.png|200px|center|frame]]<br />
<br />
[[Image:Lambert_GA_team.png|right|frame|Your team picture]]<br />
<br />
<!--- Team Information Link ---><br />
<br />
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[https://igem.org/Team.cgi?year=2013&division=high_school&team_name=Lambert_GA Official Team Profile]<br />
|}<br />
<br />
=Intro=<br />
Tell us about your team, your school!<br />
<br />
The Lambert High School iGem Team was first started in 2013 by Mrs.Standeven... (Insert rest of information)<br />
<br />
==Science! Link ==<br />
[[https://2014hs.igem.org/Science!]]<br />
<br />
==Methods==<br />
Show us how you spent your days.<br />
<br />
===Methods 1===<br />
<br />
===Methods 2===<br />
<br />
=Results=<br />
What did you achieve over the course of your semester?<br />
<br />
=Future Work=<br />
<br />
=Safety=<br />
- '''All''' team members were trained in appropriate techniques for dealing with cell culture, recombinant DNA, and general lab safety.<br />
<br />
- '''All''' lab procedures were done under the supervision of the instructors.<br />
<br />
- '''All''' experiments with recombinant DNA were approved by Dr. Mark Styczynski from the Georgia Institute of Technology.<br />
<br />
- '''All''' disposal of lab waste was treated in accordance with the MSDS or appropriate sterilization of biohazards.<br />
<br />
=Human Practices=<br />
What impact does/will your project have on the public? <br />
<br />
=Acknowledgements=<br />
<br />
'''Our thanks goes out to:'''<br />
<br />
'''-''' ''Dr. Mark Styczynski''<br />
<br />
'''-''' ''Georgia Institute of Technology and his Team''<br />
<br />
'''-''' ''The NSF grant #1254382''<br />
<br />
'''-''' ''New England Biolabs and Chris Cook''<br />
<br />
'''-''' ''Chik fila John’s Creek''<br />
<br />
'''-''' ''Lambert High School and Principal Dr. Gary Davison''<br />
<br />
'''-''' ''The Ge Family''<br />
<br />
'''-''' ''Jaemor Farms''<br />
<br />
'''-''' ''Northside Hospital Forsyth''<br />
<br />
'''-''' and ''III Below Films''.<br />
<br />
'''The Lambert High School iGem team would've never have gotten this far without all of their help and support.'''<br />
<br />
=Fun!=<br />
What was your favorite team snack?? Have a picture of your team mascot? <br />
<br />
(Insert Video?)<br />
<br />
<forum_subtle /></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/ProjectTeam:Lambert GA/Project2014-06-14T15:00:25Z<p>Kahanpparekh: </p>
<hr />
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<div id="menu"><br />
<ul><br />
<li class="first"><a href="https://2013hs.igem.org/Team:Lambert_GA" accesskey="1" title="">Home</a></li><br />
<li><a href="https://2013hs.igem.org/Team:Lambert_GA/team" accesskey="3" title="">Team</a></li><br />
<li><a href="https://2013hs.igem.org/Team:Lambert_GA/project" accesskey="2" title="">Project</a></li><br />
<li><a href="https://2013hs.igem.org/Team:Lambert_GA/labnotebook" accesskey="4" title="">Lab Notebook</a></li><br />
<li><a href="https://2013hs.igem.org/Team:Lambert_GA/resultsconclusions" accesskey="5" title="">Conclusions</a></li><br />
<li><a href="https://2013hs.igem.org/Team:Lambert_GA/futureaspirations" accesskey="8" title="">Future</a></li><br />
<li><a href="https://2013hs.igem.org/Team:Lambert_GA/safety" accesskey="5" title="">Safety</a></li><br />
<li><a href="https://2013hs.igem.org/Team:Lambert_GA/attributions" accesskey="6" title="">Attributions</a></li><br />
<li><a href="https://2013hs.igem.org/Team:Lambert_GA/humanpractices" accesskey="7" title="">Human Practices</a></li> <br />
<li><a href="https://2013hs.igem.org/Team:Lambert_GA/activities" accesskey="6" title="">Activities</a></li> <br />
</ul><br />
<br />
</head><br />
<body><br />
<br />
<font size=4><center><b><u><h1>Project</h1></u></b></center></font><br />
<br />
<center><img src="https://fbcdn-sphotos-a-a.akamaihd.net/hphotos-ak-prn1/p526x296/1016042_213819975433663_2086842284_n.jpg" width="450"/></center><br />
<br />
<font size=3><br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;In 2010, Georgia Institute of Technology’s iGem team submitted the part K410000, a periplasmic heat generator made with HybB and OmpA and AOX. The following year several teams used HybB in their projects with mixed results. Our project is built off of these projects and it's purpose is to characterize and Biobrick HybB, a cold shock promoter.A Biobrick is a sequence of DNA that has standard prefixes and suffixes. Our goal is to test the reliability of HypB, the cold shock promoter, in order to use temperature to regulate protein expression. Our test will be to put RFP under HybB promotion and give it a cold shock treatment, which should enable HybB to act as a switch and turn on the red fluorescent protein in the <i> E. Coli</i> cells, if the HybB cells are reliable. We have decided to use HybB, even though it yeilded mix results in the past, because this cold shock promoter showed potential.<br><br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;HybB was synthesized for the first time by the 2005 UCSF iGEM team. The 2006 MIT iGEM team standardized HybB and submitted it to the parts registry. HybB has the part number J45503 and is not available from the Parts Registry. Georgia Tech’s 2010 iGEM team engineered a chassis to include HybB, OmpA, and AOX. The 2011 Leuven iGEM team reported, “We see that promoter activity is induced when cells are transferred to 25°C and even when they are put in an ice bath (4°C). Unfortunately, however, cells that are kept at 37°C also display an increase in promoter activity, indicating leakiness in the system.” The 2011 Groningen team reported that the HybB Promoter did not work. Through sequencing, they proved that HybB was found in the cells, and they ran the reactions at the same temperatures.However, they could not get the same results as the Leuven team. Due to these more recent findings, we are not sure whether HybB works or not.<br><br>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We obtained HybB from Georgia Tech's 2010 iGEM Team, but upon sequencing and experimentation, we learned that the stock was not reliable. We then designed primers to isolate the proposed HybB promoter sequence from the <i>E. Coli</i> DH10 genome. </font><br />
</html><br />
<br />
<center>http://ecocar.gatech.edu/wp-content/uploads/2010/11/buzz.jpg</div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/WIPHomePageTeam:Lambert GA/WIPHomePage2014-06-14T14:06:02Z<p>Kahanpparekh: </p>
<hr />
<div><font size="7">L</font> <font size="5">ambert</font> <font size="7">GA</font><br />
<br />
Lambert high school’s iGem team is working with a chitosan based anti-fungal that stops fungi from feeding off the saccharides on the surface, and inside the fruit, which causes rotting. The chitosan, has proven harder to biologically produce. It is currently produced with chemical intensive process that doesn't always create the correct type of chitosan. Chitosan is a deacetylated form of chitin, a very common organic compound found in cell walls. Chitosan is naturally produced in nature as a food source for many organisms. This is due to the protein chitin deacetylase (CDA) which removes an acetyl group from chitin. Our goals is to extract the CDA gene from the organism Saccharomyces cerevisiae, a very well-known strain of yeast, and express it in E. coli to mass produce the enzyme and create chitosan. Lambert iGem is also planning on creating a BioBrick of the CDA gene for future implications. <br />
<br><br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/WIPHomePageTeam:Lambert GA/WIPHomePage2014-06-11T00:38:58Z<p>Kahanpparekh: </p>
<hr />
<div><font size="7">L</font> <font size="5">ambert</font> <font size="7">GA</font><br />
<br />
<b><i>Work in Progress of HomePage Development.</i></b><br />
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</html></div>Kahanpparekhhttp://2014hs.igem.org/Team:Lambert_GA/WIPHomePageTeam:Lambert GA/WIPHomePage2014-05-29T21:35:34Z<p>Kahanpparekh: </p>
<hr />
<div><font size="7">L</font> <font size="5">ambert</font> <font size="7">GA</font><br />
<br />
<b><i>Work in Progress of HomePage Development.</i></b><br />
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<hr />
<div><font size="7">L</font> <font size="5">ambert</font> <font size="7">GA</font><br />
<br />
<b><i>Work in Progress of HomePage Development.</i></b><br />
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<hr />
<div><font size="7">L</font> <font size="5">ambert</font> <font size="7">GA</font><br />
<br />
<b><i>Work in Progress of HomePage Development.</i></b><br />
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<hr />
<div><font size="7">L</font> <font size="5">ambert</font> <font size="7">GA</font><br />
<br />
<b><i>Work in Progress of HomePage Development.</i></b><br />
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</html></div>Kahanpparekh