http://2014hs.igem.org/wiki/index.php?title=Special:Contributions&feed=atom&limit=20&target=Peiwen+cai&year=&month=2014hs.igem.org - User contributions [en]2024-03-29T11:18:21ZFrom 2014hs.igem.orgMediaWiki 1.16.5http://2014hs.igem.org/File:Fun1.jpgFile:Fun1.jpg2014-06-20T17:31:16Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T17:28:18Z<p>Peiwen cai: /* Fun */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br><br />
[[File:kanshu.png|500px]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br><br />
[[File:Shenzhen_mengtu.png|600px]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br><br />
[[File:jianmomengtu.png|500px]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br><br />
[[File:anquan.png|500px]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/File:2333.jpgFile:2333.jpg2014-06-20T17:27:24Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T17:22:52Z<p>Peiwen cai: /* Fun */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br><br />
[[File:kanshu.png|500px]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br><br />
[[File:Shenzhen_mengtu.png|600px]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br><br />
[[File:jianmomengtu.png|500px]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br><br />
[[File:anquan.png|500px]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br><br />
[[File:2333.jpg|500px]]<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/File:23333.jpgFile:23333.jpg2014-06-20T17:22:06Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T17:20:09Z<p>Peiwen cai: /* Fun */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br><br />
[[File:kanshu.png|500px]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br><br />
[[File:Shenzhen_mengtu.png|600px]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br><br />
[[File:jianmomengtu.png|500px]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br><br />
[[File:anquan.png|500px]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br><br />
[[File:23333.jpg|500px]]<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T17:19:39Z<p>Peiwen cai: /* Fun */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br><br />
[[File:kanshu.png|500px]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br><br />
[[File:Shenzhen_mengtu.png|600px]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br><br />
[[File:jianmomengtu.png|500px]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br><br />
[[File:anquan.png|500px]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br><br />
[[File:23333.png|500px]]<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T17:18:09Z<p>Peiwen cai: /* Modelling */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br><br />
[[File:kanshu.png|500px]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br><br />
[[File:Shenzhen_mengtu.png|600px]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br><br />
[[File:jianmomengtu.png|500px]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br><br />
[[File:anquan.png|500px]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/File:Anquan.pngFile:Anquan.png2014-06-20T17:17:44Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T17:16:28Z<p>Peiwen cai: /* Safety */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br><br />
[[File:kanshu.png|500px]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br><br />
[[File:Shenzhen_mengtu.png|600px]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br><br />
[[File:jianmomengtu.png|600px]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br><br />
[[File:anquan.png|500px]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/File:Jianmomengtu.pngFile:Jianmomengtu.png2014-06-20T17:14:32Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T17:12:56Z<p>Peiwen cai: /* Modelling */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br><br />
[[File:kanshu.png|500px]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br><br />
[[File:Shenzhen_mengtu.png|600px]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br><br />
[[File:jianmomengtu.png|600px]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/File:Kanshu.pngFile:Kanshu.png2014-06-20T17:08:32Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T17:06:39Z<p>Peiwen cai: /* Project */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br><br />
[[File:kanshu.png|500px]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br><br />
[[File:Shenzhen_mengtu.png|600px]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T16:59:25Z<p>Peiwen cai: /* Lab */</p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br><br />
[[File:Shenzhen_mengtu.png|600px]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/File:Shenzhen_mengtu.pngFile:Shenzhen mengtu.png2014-06-20T16:58:51Z<p>Peiwen cai: </p>
<hr />
<div></div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMSTeam:Shenzhen SZMS2014-06-20T16:55:31Z<p>Peiwen cai: </p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
{| align=right<br />
|-<br />
|[[File:IGEM basic Logo white stylized.png|100px]]<br />
|[[File:Shenzhen_SZMS_logo.png|100px]]<br />
|}<br />
[[File:Shenzhongigem2014.png|400px|right]]<br />
Welcome to homepage of '''Team:Shenzhen SZMS'''! Our project is called '''E. coli Plant-sitter'''. <br />
<br />
==Team==<br />
Shenzhen Middle School (深圳中学 Shēn Zhèn Zhōng Xué) is an high school in Luohu District, Shenzhen, Guangdong ever since 1947. In December 2013, a group of 17 SZMS students who have enthusiasm in biology were gathered together in the little yet omnipotent lab on campus; ever since then, this team of 17, Shenzhen SZMS has been established.<br />
<br />
===Attributions===<br />
Hey here! Check out our 17 members![[Team:Shenzhen SZMS/Team Members]].<br />
<br />
==Project==<br />
Our project is to create an “E. coli Plant-sitter” that achieves smart maintenance of healthy plant growth through the application of synthetic biology to temperature moderation, which can be applied to either hothouse cultivation on Earth or the Human Migration Program on MARS.<br />
<br />
Read more:[[Team:Shenzhen SZMS/Project]]<br />
<br />
==Lab==<br />
Wanna find more about how we bulid our plasmid? Check here:[[Team:Shenzhen SZMS/Lab]]<br><br />
[[File:Shenzhen_mengtu.png|100px]]<br />
<br />
==Modelling==<br />
We bulit a model to estimate how the enzyme works. Check here:[[Team:Shenzhen SZMS/Modelling]]<br />
<br />
==Safety==<br />
Check our answers to the Safety Questions here:[[Team:Shenzhen SZMS/iGem Safety Q&A]]<br />
<br />
==Human Practice==<br />
{| align=right<br />
|[[File:helloworld1.jpeg|200px]]<br />
|-<br />
|[[File:helloworld2.jpeg|200px]]<br />
|-<br />
|[[File:helloworld3.jpeg|200px]]<br />
|-<br />
|[[File:helloworld4.jpeg|200px]]<br />
|}<br />
<br />
Come and meet the friend of our E.coli babysitter,''' E.coli the Fluorescent'''!<br />
<br />
Sincere greeting from the iGem lab in Shenzhen Middle School!<br />
<br />
Since all of our ''' E.coli the Fluorescent''' are brilliant in both languages, the fourth group of them are advertising for their friend, E.coli Plant-sitter: "The soulmate of plant, E.coli babysitter hug you warm and give you heat all the time."<br />
<br />
Our human practice plan includes:<br />
#Take part in [http://www.dnaday.com/default.asp BIT's 5th World Annual World DNA and Genome Day] in Dalian from April 25 to 28, 2014. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]<br />
#Open our laboratory and invite students in our school to come and experience what we usually do, such as agarose gel electrophoresis. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]<br />
#Hold lectures at junior high schools in Shenzhen to encourage junior high school students to love biology. <br>'''Details''':[[Team:Shenzhen SZMS/Human Practice/Lectures]]<br />
<br />
==Fun==<br />
Theme song for our project called [[Team:Shenzhen SZMS/E.coli Plant-sitter heals the world|''E.coli Plant-sitter heals the world'']]! A song that always arises in our minds whenever we think of iGem :)<br />
<br />
Find more photos here:[[Team:Shenzhen SZMS/Gallery]]!<br />
<br />
==More Info==<br />
*[http://www.shenzhong.net/ school offical website (in Chinese)] <br />
*[http://www.shenzhong.net/news/2013314/n73836977.html school intro (in English)]<br />
*[http://en.wikipedia.org/wiki/Shenzhen_Middle_School school info on Wikipedia]</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMS/GalleryTeam:Shenzhen SZMS/Gallery2014-06-20T16:03:47Z<p>Peiwen cai: </p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
[[File:Shenzhen_SZMS_logo.png|right|150px|Shenzhen Middle School]]<br />
Here are some pictures of [[Team:Shenzhen SZMS]].<br />
<br />
== Photos ==<br />
{| align=center<br />
|align=center|[[File:9L7A3288.JPG|400px|Some of the team members are talking about the project. April 9, 2014]]<br />
|align=center|[[File:9L7A3647.JPG|400px|Zhang Yang is checking the cell culture.]]<br />
|-<br />
|align=center|[[File:SZMS 9L7A0141.JPG|400px|Some colorful bacteria]]<br />
|align=center|[[File:SZMS 9L7A0357.JPG|400px|Mr.Wang from Beijing Genomics Institute is teaching us. March 12, 2014]]<br />
|}<br />
{| align=center<br />
|align=center|[[File:helloworld1.jpeg|220px|E.coli the Fluorescent]]<br />
|align=center|[[File:helloworld2.jpeg|220px|E.coli the Fluorescent]]<br />
|align=center|[[File:helloworld3.jpeg|220px|E.coli the Fluorescent]]<br />
|align=center|[[File:helloworld4.jpeg|220px|E.coli the Fluorescent]]<br />
|}<br />
{| align=center<br />
|<br />
{|<br />
|align=center|[[File:SZMS (4).JPG|600px]]<br />
|-<br />
|align=center|[[File:SZMS (8).JPG|600px]]<br />
|}<br />
|<br />
{|<br />
|align=center|[[File:SZMS (1).JPG|200px]]<br />
|-<br />
|align=center|[[File:SZMS (2).JPG|200px]]<br />
|-<br />
|align=center|[[File:SZMS (3).JPG|200px]]<br />
|-<br />
|align=center|[[File:SZMS (9).JPG|200px]]<br />
|-<br />
|align=center|[[File:SZMS (6).JPG|200px]]<br />
|-<br />
|align=center|[[File:SZMS (7).JPG|200px]]<br />
|}<br />
|}<br />
=== Lectures in Secondary Schools ===<br />
''Learn more:[[Team:Shenzhen SZMS/Human Practice/Lectures]]''<br />
{| align=center<br />
|align=center|[[File:SZMS panel -1.png|300px]]<br />
|align=center|[[File:SZMS panel -2.png|300px]]<br />
|align=center|[[File:SZMS panel -3.png|300px]]<br />
|}<br />
{| align=right<br />
|align=center|[[File:Chuzhongbu1.jpg|400px]]<br />
|align=center|[[File:Chuzhongbu2.jpg|400px]]<br />
|}<br />
{| align=center<br />
|align=center|[[File:SZMS's lecture at junior high part of Shenzhen Middle School (1).jpg|200px]]<br />
|align=center rowspan=2|[[File:SZMS's lecture at junior high part of Shenzhen Middle School (8).jpg|400px]]<br />
|align=center|[[File:SZMS's lecture at junior high part of Shenzhen Middle School (2).jpg|200px]]<br />
|-<br />
|align=center|[[File:SZMS's lecture at junior high part of Shenzhen Middle School (3).jpg|200px]]<br />
|align=center|[[File:SZMS's lecture at junior high part of Shenzhen Middle School (4).jpg|200px]]<br />
|}<br />
{| align=left<br />
|align=center rowspan=2|[[File:SZMS's lecture at junior high part of Shenzhen Middle School (10).jpg|300px]]<br />
|align=center|[[File:SZMS's lecture at junior high part of Shenzhen Middle School (9).jpg|300px]]<br />
|align=center|[[File:SZMS's lecture at junior high part of Shenzhen Middle School (6).jpg|300px]]<br />
|-<br />
|align=center|[[File:SZMS's lecture at junior high part of Shenzhen Middle School (11).jpg|300px]]<br />
|align=center|[[File:SZMS's lecture at junior high part of Shenzhen Middle School (7).jpg|300px]]<br />
|}<br />
{| align=right<br />
|align=center|[[File:Lianhua1.jpg|300px]]<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (5).jpg|300px]]<br />
|align=center|[[File:Lianhua2.jpg|300px]]<br />
|}<br />
{| align=center<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (8).jpg|200px]]<br />
|align=center rowspan=2|[[File:SZMS's lecture at Lianhua Middle School (6).jpg|400px]]<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (7).jpg|200px]]<br />
|-<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (11).jpg|200px]]<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (10).jpg|200px]]<br />
|-<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (12).jpg|200px]]<br />
|align=center rowspan=2|[[File:SZMS's lecture at Lianhua Middle School (4).jpg|400px]]<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (14).jpg|200px]]<br />
|-<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (13).jpg|200px]]<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (21).jpg|200px]]<br />
|}<br />
{|<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (18).jpg|300px]]<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (19).jpg|300px]]<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (20).jpg|300px]]<br />
|}<br />
{| align=right<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (1).jpg|300px]]<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (2).jpg|300px]]<br />
|align=center|[[File:SZMS's lecture at Lianhua Middle School (3).jpg|300px]]<br />
|}<br />
<br style="clear:both;"><br />
<br />
=== Laboratory Open Day ===<br />
''Learn more:[[Team:Shenzhen SZMS/Human Practice/Laboratory Open Day]]''<br />
{| align=center<br />
|align=center|[[File:SZMS_Laboratory_Open_Day_poster_-1.png|400px]]<br />
|align=center|[[File:SZMS_Laboratory_Open_Day_poster_-2.png|400px]]<br />
|}<br />
{| align=center<br />
|align=center|[[File:Kaifangri1.jpg|300px|Laboratory Open Day]]<br />
|align=center|[[File:Kaifangri2.jpg|300px|Laboratory Open Day]]<br />
|align=center|[[File:Kaifangri3.jpg|300px|Laboratory Open Day]]<br />
|-<br />
|align=center|[[File:Kaifangri4.jpg|300px|Laboratory Open Day]]<br />
|align=center|[[File:Kaifangri5.jpg|300px|Laboratory Open Day]]<br />
|align=center|[[File:Kaifangri6.jpg|300px|Laboratory Open Day]]<br />
|}<br />
{| align=center<br />
|<br />
{|<br />
|align=center|[[File:SZMS (21).JPG|200px]]<br />
|-<br />
|align=center|[[File:SZMS (15).JPG|200px]]<br />
|-<br />
|align=center|[[File:SZMS (17).JPG|200px]]<br />
|-<br />
|align=center|[[File:SZMS (18).JPG|200px]]<br />
|-<br />
|align=center|[[File:SZMS (19).JPG|200px]]<br />
|-<br />
|align=center|[[File:SZMS (20).JPG|200px]]<br />
|}<br />
|<br />
{|<br />
|align=center|[[File:SZMS (14).JPG|600px]]<br />
|-<br />
|align=center|[[File:SZMS (16).JPG|600px]]<br />
|}<br />
|}<br />
<br style="clear:both;"><br />
=== World DNA and Genome Day ===<br />
''Learn more:[[Team:Shenzhen SZMS/Human Practice/BIT's 5th World DNA and Genome Day]]''<br />
{| align=center<br />
|align=center|[[File:SZMS poster for BIT's 5th World DNA and Genome Day (1).JPG|400px|An English poster for World DNA and Genome Day]]<br />
|align=center|[[File:SZMS poster for BIT's 5th World DNA and Genome Day (2).JPG|400px|An Chinese poster for World DNA and Genome Day]]<br />
|}<br />
{| align=center<br />
|align=center|[[File:Dalian1.jpg|300px|BIT's 5th World DNA and Genome Day]]<br />
|align=center|[[File:Dalian2.jpg|300px|BIT's 5th World DNA and Genome Day]]<br />
|align=center|[[File:Dalian3.jpg|300px|BIT's 5th World DNA and Genome Day]]<br />
|-<br />
|align=center|[[File:SZMS with Ada Yonath.JPG|300px|SZMS with Ada Yonath]]<br />
|align=center|[[File:SZMS with Akira Suzuki.JPG|300px|SZMS with Akira Suzuki]]<br />
|align=center|[[File:SZMS with Dan Shechtman.JPG|300px|SZMS with Dan Shechtman]]<br />
|}<br />
{|<br />
|align=center|[[File:SZMS with George F. Smoot.JPG|400px|SZMS with George F. Smoot]]<br />
|align=center|[[File:SZMS with Hartmut Michel.JPG|400px|SZMS with Hartmut Michel]]<br />
|}<br />
{| align=right<br />
|align=center|[[File:SZMS with Luc Montagnier.JPG|400px|SZMS with Luc Montagnier.JPG]]<br />
|align=center|[[File:SZMS with Vladimir Khavinson.JPG|400px|SZMS with Luc Montagnier.JPG]]<br />
|}<br />
<br style="clear:both;"><br />
<br />
== Our cuties ==<br />
{| align=right<br />
|align=center|[[File:SZMS brochure 3.jpg|150px]]<br />
|align=center|[[File:Shenzhongigem2014.jpg|400px]]<br />
|align=center|[[File:Albumface.jpg|400px]]<br />
|}<br />
{| align=center<br />
|align=center|[[File:Huoxing1.jpg|220px|E.coli on Mars]]<br />
|align=center|[[File:Huoxing3.jpg|220px|E.coli on Mars]]<br />
|align=center|[[File:Huoxing4.jpg|220px|E.coli on Mars]]<br />
|align=center|[[File:Huoxing2.jpg|220px|E.coli on Mars]]<br />
|}<br />
{| align=center<br />
|align=center|[[File:SZMS brochure 2.jpg|175px]]<br />
|align=center|[[File:SZMS brochure 4.jpg|175px]]<br />
|align=center|[[File:SZMS brochure 5.jpg|175px]]<br />
|align=center|[[File:SZMS brochure 6.jpg|175px]]<br />
|align=center|[[File:SZMS brochure 7.jpg|175px]]<br />
|}</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMS/iGem_Safety_Q%26ATeam:Shenzhen SZMS/iGem Safety Q&A2014-06-20T16:03:34Z<p>Peiwen cai: </p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
Here are our answers for [https://2014hs.igem.org/Safety iGEM Safety Questions].<br />
<br />
==Q&A==<br />
===Question 1===<br />
1.Would any of your project ideas raise safety issues in terms of:<br />
*researcher safety,<br />
*public safety, or<br />
*environmental safety'''<br />
----<br />
Organisms:<br />
<br />
:E.coli<br />
:#Risk Group 1 (no or low individual and community risk) - WHO Laboratory Biosafety Manual<br />
:#BSL 1 - The list of human to human transmission of pathogenic microorganisms<br />
<br />
:Scindapsus aureus<br />
:#Risk Group 1 (no or low individual and community risk) - WHO Laboratory Biosafety Manual<br />
<br />
Risks:<br />
#Potential pathogenicity to team members since e.coli is infectant and would cause disease when accumulating.<br />
#Potential risk of the release of modified gene out of the lab and possible pollution of other organisms.<br />
<br />
Addressing issues:<br />
#Suicide system<br />
#Lab Safety Rules: Good microbiological techniques(GMT)<br>General requirements for laboratory biosafety GB-19489 2008<br />
<br />
Safety training:<br />
#WHO Laboratory Biosafety Manual<br />
#General requirements for laboratory biosafety GB-19489 2008<br />
#Biosafety in the Microbiological and Biomedical Laboratories Manual,3th edition CDC/NIH <br />
<br />
Accident out of plan:<br />
#Release of modified gene and possible genetic pollution if the containment procedures go wrong.<br />
#Release of bacteria and possible health problem of team members.<br />
<br />
Addressing issues:<br />
#Suicide system to prevent genetic pollution.<br />
#Protection suits for researchers.<br />
#Medical examination in hospital.<br />
#Complete sterilization in the lab.<br />
<br />
=== Question 2 ===<br />
2.Do any of the new BioBrick parts (or devices) that you made this year raise safety issues? If yes,<br />
*Did you document these issues in the Registry?<br />
*How did you manage to handle the safety issue?<br />
*How could other teams learn from your experience?<br />
----<br />
All the biosafety levels of the parts in our project are BSL 1.<br />
<br />
Suicide system<br />
<br />
=== Question 3 ===<br />
3.Is there a local biosafety group, committee, or review board at your institution?<br />
*If yes, what does your local biosafety group think about your project?<br />
*If no, which specific biosafety rules or guidelines do you have to consider in your country?<br />
----<br />
No.<br />
<br />
WHO Laboratory Biosafety Manual<br />
<br />
General requirements for laboratory biosafety GB-19489 2008<br />
<br />
Biosafety in the Microbiological and Biomedical Laboratories Manual,3th edition CDC/NIH<br />
<br />
<br />
=== Question 4 ===<br />
4.Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?<br />
----<br />
#Safety level examination of parts and devices in lab.<br />
#Clear biosafety hierarchy system of BioBrick parts(or devices) and publish the classification on the website of iGem for participants to consult.<br />
#Suicide system to prevent genetic pollution when release of organism happens.<br />
<br />
==Reference==<br />
#''The list of human to human transmission of pathogenic microorganisms'';<br />
#''General requirements for laboratory biosafety GB-19489 2008''.</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMS/LabTeam:Shenzhen SZMS/Lab2014-06-20T16:03:22Z<p>Peiwen cai: </p>
<hr />
<div>{| class="wikitable" align=center<br />
|align=center width=15%|[[Team:Shenzhen SZMS|Home]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Project|Project]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Modelling|Modelling]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/iGem Safety Q&A|Safety]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
Here you can find out how we build our plasmid. The plasmid map and information of parts used is given in [[Team:Shenzhen SZMS/Project]].<br />
<br />
==Schedule==<br />
{| class="wikitable"<br />
|-<br />
!width=50px|No. !!width=300px| Procedure !!width=100px|Backbone !!width=250px| Status of the Plasmid<br />
|-<br />
|1<br />
|[K115016] Spe1,Pst1 Double Digestion, Electrophoresis detection,DNA refinement;[C0051] X ba1,Pst1Double Digestion, Gel extraction;Combination<br />
|1C3(K115016)<br />
|[K115016+C0051]<br />
|-<br />
|2<br />
|[J23106] Spe1,Pst1 Double Digestion, Electrophoresis detection,DNA refinement;[K115016+C0051] X ba1,Pst1Double Digestion, Gel extraction;Combination<br />
|1A2(J23106)<br />
|J23106+[K115016+C0051]<br />
|-<br />
|3<br />
|[J2s3106+ K115016+C0051] EcoR1,Spe1Double Digestion, Electrophoresis detection,DNA refinement;[B0051] EcoR1,Xba1 Electrophoresis detection,DNA refinement;Combination<br />
|1C3(B0015)<br />
|[J23106+K115016+C0051]+B0015<br />
|-<br />
|4<br />
|[B0030] Spe1,Pst1 Double Digestion, Electrophoresis detection, DNA refinement;[EC:1.7.7.2] Xba1, Pst1Double Digestion, Gel extraction;Combination<br />
|1A2(B0030)<br />
|B0030+EC:1.7.7.2<br />
|-<br />
|5<br />
|[R0051] Spe1, Pst1Double Digestion, Electrophoresis detection,DNA refinement;[B0030+ EC:1.7.7.2] Xba1,Pst1 Double Digestion, Gel extraction;Combination<br />
|1A2(R0051)<br />
|R0051+[B0030+ EC:1.7.7.2]<br />
|-<br />
|6<br />
|[B0030] Spe1, Pst1Double Digestion, Electrophoresis detection,DNA refinement;[K145015] Xba1,Pst1Double Digestion, Gel extraction; Combination<br />
|1A2(B0030)<br />
|B0030+K145015<br />
|-<br />
|7<br />
|[B0030+ K145015] EcoR1, Spe1Double Digestion, Electrophoresis detection,DNA refinement;[B0015] EcoR1, Xba1Double Digestion,Electrophoresis detection,DNA refinement;Combination<br />
|1C3(B0015)<br />
|[B0030+ K145015]+B0015<br />
|-<br />
|8<br />
|[R0051+B0030+ EC:1.7.7.2] Spe1, Pst1Double Digestion,Gel extraction;[B0030+ K145015+B0015]Xba1, Pst1 Gel extraction;Combination<br />
|1C3(B0030+K145015+B0015)<br />
|[R0051+B0030+ EC:1.7.7.2]+[B0030+K145015+B0015]<br />
|-<br />
|9<br />
|[K115017] Spe1, Pst1Double Digestion, Electrophoresis detection,DNA refinement;[C0051] Xba1, Pst1Double Digestion,Gel extraction; Combination<br />
|1C3(K115016)<br />
|[K115017+C0051]<br />
|-<br />
|10<br />
|[J23106] Spe1,Pst1Double Digestion, Electrophoresis detection,DNA refinement;[K115017+C0051] Xba1, Pst1Double Digestion, Gel extraction;Combination<br />
|1A2(J23106)<br />
|J23106+[K115017+C0051]<br />
|-<br />
|11<br />
|[J23106+ K115017+C0051] EcoR1, Spe1Double Digestion,Gel extraction;[B0015]EcoR, Xba1Double Digestion, Electrophoresis detection,DNA refinement,Combination<br />
|1C3(B0015)<br />
|[J23106+ K115017+C0051]+B0015<br />
|-<br />
|12<br />
|[The first part] Spe1,Pst1 Double Digestion;[The second part] Xba1,PstL Double Digestion;Gel extraction; Combination<br />
|1C3(The first part)<br />
|1st+2nd<br />
|-<br />
|13<br />
|[The first and second parts] Spe1、Pst1Double Digestion; [The third part] Xba1、Pst1Double Digestion;Gel extraction; Combination<br />
|1C3(The third part)<br />
|1st +2nd+3rd<br>Done<br />
|}<br />
<br />
==Log Book==<br />
{| class="wikitable"<br />
|-<br />
!width=50px| Date !!width=300px| Event !!width=300px| Results !!width=200px| Problem summary<br />
|-<br />
|Apr.3<br />
|B0015(chl) B0030(chl) Plasmid Transformation<br />
|Done <span style="color:grey">(yet this B0030 is single error)</span><br />
|<br />
|-<br />
|Apr.4<br />
|Single clone selected from the work on Apr.3 <span style="color:red">(In order to be pithy, this process is omitted from the table in all the following daily work)</span><br />
|Done<br />
|<br />
|-<br />
|Apr.5<br />
|Plasmid preped and culture restored from the bacterial suspension on Apr.4 <span style="color:red">(omitted later for pithiness and convenience as well)</span><br />
|Done<br />
|<br />
|-<br />
|Apr.6<br />
|K115016(CHL) K115017(CHL) C0051(CHL) transformation<br />
|Done<br />
|<br />
|-<br />
|May.2<br />
|J23106(AMP) K082003(CHL,as GFP+LVA) transformation (help offered by Mentor Wang)<br />
|Done<br />
|<br />
|-<br />
|May.5<br />
|Digestion: K115016(S/P) C0051(X/P) C0051(E/S) B0015(E/X)<br>Electrophoresis<br>Gel extraction<br>16℃ overnight<br />
|Done<br />
|<br />
|-<br />
|May.6<br />
|Product from 5.5(CHL)transformed<br />
|Failed<br />
|Might be the problem during the plasmid prep<br />
|-<br />
|May.7<br />
|Plasmid mentioned above repreped<br />
|Done<br />
|<br />
|-<br />
|May.13<br />
|Digestion: C0051(E/S) B0015(S/P) K082003(E/S)<br>Electrophoresis<br>Gel extraction<br>16℃ overnight<br />
|Done, C0051-B0015<br>K082003-B0015 gained<br />
|<br />
|-<br />
|May.14<br />
|Products from 5.13(CHL)all transformed<br />
|Done, C0051-B0015<br>K082003-B0015 gained<br />
|<br />
|-<br />
|May.15<br />
|Gel confirmation (products from 5.14), comparison with gel of B0015<br />
|Done, product longer than the original plasmid<br />
|<br />
|-<br />
|May.27<br />
|Digestion: C0051-B0015(X/P) K115016(S/P) K115017(S/P)<br>Electrophoresis<br>Gel extraction<br>Combination<br>Transformation(CHL)<br />
|Done,<br>K115016-C0051-B0015<br>K115017-C0051-B0015<br>gained.<br />
|<br />
|-<br />
|May.28<br />
|B0030(AMP) transformation<br />
|Done; informed that R0051 requires PCR in order to get the primer of EC 1.7.7.2<br />
|<br />
|-<br />
|May.29<br />
|Digestion: J23106(S/P)<br>K115016-C0051-B0015(X/P)<br>K115017-C0051-B0015(X/P)<br>Electrophoresis<br>Gel extraction<br>Combination<br>Transformation(AMP)<br />
|Done,<br>J23106-K115016-C0051-B0015(27℃CI-protein)<br>J23106-K115017-C0051-B0015(32℃CI-protein)<br>gained.<br />
|<br />
|-<br />
|May.30<br />
|The result of sequencing of C0051-B0015<br>K082003-B0015 received<br />
|Correctly combined<br />
|<br />
|-<br />
|Jun.2<br />
|Digestion: B0030(S/P) K082003-B0015(X/P)<br>Electrophoresis<br>Gel extraction<br>Combination<br>Transformation(AMP)<br />
|Done<br />
|<br />
|-<br />
|Jun.8<br />
|The results of sequencing of J23106-K115016-C0051-B0015(27℃CI) and J23106-K115017-C0051-B0015(32℃ CI)received.<br />
|Correctly combined<br />
|<br />
|-<br />
|Jun.9<br />
|Digestion:B0030-K082003-B0015(E/S)<br>J23106-K115017-C0051-B0015(E/X)<br>Electrophoresis<br>Gel extraction<br>Combination<br>Transformation(AMP)<br />
|Done with transformation;<br>Yet failed in the electrophoretic analysis.<br />
|Disconnected<br />
|-<br />
|Jun.10<br />
|<br />
|ciF1 ciF2 ciF3: primers to synthesize EC 1.7.7.2 and PEP (additional PEP R0051-B0030-) gained.<br />
|<br />
|-<br />
|Jun.11<br />
|With the original blue 2X Master Mix PCR round 1,2,3 implemented<br />
|Failed; only primer founded in the electrophoretic analysis<br />
|<br />
|-<br />
|Jun.12<br />
|Round 1 from 6.11 repeated in the morning;<br>Round 3 PCR with 2X hi-fi Master Mix at noon<br />
|Failed in the morning but done in the afternoon;<br>R0051-B0030-1.7.7.2 recycled product gained.<br />
|might be the problem of MIX<br />
|-<br />
|Jun.13<br />
|Digestion: 27℃ CI(S/P)R0051-B0030-1.7.7.2(X/P)<br>32℃ CI(E/X) <br>B0030-K082003-B0015(E/S)<br>Electrophoresis<br>Gel<br>extraction<br>Combination<br>Transformation.<br />
|Failed, no culture founded on the dish.<br />
|Unknown error; might be the problem of extracted gel.<br />
|-<br />
|Jun.15 <br />
|Digestion: 27℃ CI(S/P)R0051-B0030-1.7.7.2(X/P)<br>32℃ CI(E/X) <br>B0030-K082003-B0015(E/S)<br>Electrophoresis<br>Gel<br>extraction<br>Combination<br>Transformation.<br />
|According to the result of electrophoresis, the length of the digested and combined part<br> is the same as that of the primitive plasmid;<br> yet we cannot exclude the problem of plasmid prep; might have failed. <br />
|A tube was missed during the digestion yet was made up later; do not know about its possible effect yet.<br />
|}</div>Peiwen caihttp://2014hs.igem.org/Team:Shenzhen_SZMS/ModellingTeam:Shenzhen SZMS/Modelling2014-06-20T16:03:08Z<p>Peiwen cai: </p>
<hr />
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|align=center width=15%|[[Team:Shenzhen SZMS/Team Members|Team Members]]<br />
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|align=center width=15%|[[Team:Shenzhen SZMS/Lab|Lab]]<br />
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|align=center width=15%|[[Team:Shenzhen SZMS/Gallery|Gallery]]<br />
|align=center width=15%|[[Team:Shenzhen SZMS#Human Practice|Human Practice]]<br />
|}<br />
<br><br />
[[File:Cute_Plasmid1.png|100px]]<br />
[[File:Cute_Plasmid2_.png|100px]]<br />
[[File:Cute_Plasmid3.png|100px]]<br />
[[File:Cute_Plasmid4.png|150px]]<br />
[[File:Cute_Plasmid5.png|100px]]<br />
[[File:Cute_Plasmid6.png|100px]]<br />
[[File:Cute_Plasmid7.png|150px]]<br />
[[File:Cute_Plasmid8.png|100px]]<br />
[https://2014hs.igem.org/Main_Page<br> <<Back to wiki main page]<br />
----<br />
In order to assess the efficiency of our E.coli babysitter, we have made math models for the core system in it, EC 1.7.7.2, which functions as to heat up the environment to provide a more suitable temperature for the plant.<br />
<br />
EC 1.7.7.2 is basically a ferredoxin-nitrate reductase; with the required substrates nitrate and reduced ferredoxin, EC 1.7.7.2 is able to catalyze the reactions below:<br />
<br />
nitrite + H<sub>2</sub>O + oxidized ferredoxin ⇄ nitrate + reduced ferredoxin + 2H<sup>+</sup><br />
<br />
HNO<sub>2</sub> + NH<sub>3</sub> = N<sub>2</sub>↑+ 2H<sub>2</sub>O<br />
<br />
obviously as suggested above,the first reaction is a reversible one; the heat we need is actually from the second reaction since the first one is basically to convert nitrate provided by our special LB into nitrite.On the other hand, the warmth released by the first reaction is so little that it has nearly no effect on the result. Consequently,we make only the math models for the second reaction, whose substrate is nitrate. As calculated, the general warmth that would be released is 334.55 KJ/mol, which is a theoretical datum.<br />
<br />
However,considering the fact that we do not have adequate data to carry out our math models, we finally decide to use proportionality instead of accurate data. Both of the following charts are established by Excel.<br />
<br />
The first one is a model under the condition that purely only EC 1.7.7.2 is functioning, which means, the concentration of it remains unchanged: (grey line → temperature inside the systerm)<br />
<br />
[[File:nongdumeiyoubianlatest.png|800px]]<br />
<br />
The second one is a model under the condition that RNA Thermometer in the first part of our entire plasmid is functioning to start the heating-up system which is mostly EC 1.7.7.2 in certain extent of external temperature (<27℃). Since it is being manipulated by the other system, the concentration of EC 1.7.7.2 can no long remain unchanged, but changed over time: (black line → temperature inside the systerm)<br />
<br />
[[File:nongdubianleahahaha.png|800px]]<br />
<br />
General information about EC 1.7.7.2 can be found in the following link: http://www.genome.jp/dbget-bin/www_bget?ec:1.7.7.2.</div>Peiwen cai