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        Engineering<br />
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    <span style="font-family: 'Lato'; font-size: 18px;">E. Coli Capable of Extracelluar Secretion of Mycotoxin Detoxifying Enzymes</span>
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            <h2>Results</h2>
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            <p>Construction of the proposed biosensors was not completed
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              successfully due to what appear to be flawed ligations and/or low
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              efficiency competent cells. The necessary methodology and
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              procedures have been laid down for further work on construction,
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              development and improvement of the biosensor system in the future.
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              Further work include the completion of the biosensors, proof of
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              concept growing bacteria in absence and presence of stimuli and
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              application of biosensors to a range of samples from specific
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              points distributed all along Xochimilco lake. Results of these
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              tests could be then applied in the construction a map of the lake
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              specifying the most critical points that need to be treated.</p>
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            <p class="8u center">Figure 1. The agarose gel shows the unsuccesful
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    <img src="images/bannerphase4 transparent white text.png" width="1070" height="292"/>
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              ligations, represented on the right.</p>
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    <div id="third-text"> Microfungi that produce harmful mycotoxins flourish on improperly-stored nuts, grains, meat, and dairy. They especially thrive in developing countries, where the lack of advanced food storage and mycotoxin exposure causes 40% of the diseases. To lessen the problem, our team engineered E. coli strains using synthetic biology tools to produce chimeric mycotoxin-degrading fungal enzymes, Aflatoxin-Detoxifizyme (ADTZ) and Zearalenone Hydrolase (ZHD101), which are designed to be secreted to extra-cellular space by fusing with secretion signal peptides from alpha-amylase and beta-lactamase. In this study, we have successfully generated synthetic genetic materials to produce four chimeric mycotoxin-detoxifying enzymes. The levels of extracellular secretion is also characterized and analyzed. The project will allow a mass production of detoxification enzymes in cost effective way, preventing the squandering of harvested crops, and limiting mycotoxin-related diseases. Increased access to th ese proteins will have an immense commercial, industrial, agricultural, and health impact.</div> 
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                <p><em class="12u right">Winston Churchill</em></p>
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Revision as of 02:37, 21 June 2014

<!DOCTYPE html> GenetiX - Construction

Results

Construction of the proposed biosensors was not completed successfully due to what appear to be flawed ligations and/or low efficiency competent cells. The necessary methodology and procedures have been laid down for further work on construction, development and improvement of the biosensor system in the future. Further work include the completion of the biosensors, proof of concept growing bacteria in absence and presence of stimuli and application of biosensors to a range of samples from specific points distributed all along Xochimilco lake. Results of these tests could be then applied in the construction a map of the lake specifying the most critical points that need to be treated.

Figure 1. The agarose gel shows the unsuccesful ligations, represented on the right.