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    <h1 class="title">              Protocols //Notebook </h1>
 
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            <h1>Protocol 1 </h1>
 
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            <p>Preparation of culture media (1L).<br />
 
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              1.- 5 g of yeast<br />
 
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              2.- 10 g of sodium chloride.<br />
 
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              3.- 10 g of triptone.<br />
 
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              4.- Add water till 1 uL volume.</p>
 
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          </div>
 
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          <div>
 
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            <h1>Protocol 2</h1>
 
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            <p>Competent cell´s preparation<br />
 
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              <br />
 
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              1.- Centrifug your media culture tube (25 mL) at 4,000 rpm during
 
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              15 min.<br />
 
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              2.- Pour the supernatant and add 10 mL of cold MgCl2 solution.
 
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              Centrifuge them at 4,000 rpm at 4 °C during 15 min.<br />
 
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              3.- Pour the supernatant and resuspend the pellet in 40 mL of cold
 
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              CaCl2 solution. Keep it in ice during 20 min and place them in
 
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              eppendorf tubes. Centrifuge them at 4,000 rpm at 4°C during 15
 
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              min.<br />
 
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              4.- Pour the supernatant and resuspend the pellet in 40 mL of cold
 
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              85mM CaCl2/15% glycerlol v/v solution. Centrifuge again at 2,100
 
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              rpm at 4°C during 15 min.<br />
 
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              5.- Pour the supernatant and resuspend in 2 mL of cold 85bmM
 
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              CaCl2/15% glycerol v/v solution.<br />
 
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              6.- Aliquote in tubes of 100 uL previously frozen and store at
 
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              -80°C.<br />
 
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            </p>
 
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          </div>
 
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          <div>
 
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            <h1>Protocol 3</h1>
 
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            <p>DNA extraction from Buffy coat:<br />
 
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              <br />
 
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              <font size="1"> 1.- Centrifuge 5 mL of culture medium at 1,500 rpm
 
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                for 10 min at 4°C.</font><br />
 
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              <font size="1">2.- Carefully transfer 600 uL of buffy coat to a
 
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                microtube.</font><br />
 
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              <font size="1">3.- Add 1 mL of RBC buffer and mix throughly by
 
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                vortex.</font><br />
 
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              <font size="1">4.- Centrifuge at 4,500 rpm during 30 s and pour
 
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                carefully so your pellet is not wasted.</font><br />
 
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              <font size="1">5.- Repeat steps 2 and 3 adding just 500 uL from
 
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                RBC buffer.</font><br />
 
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              <font size="1">6.- Once separated, add 300 uL of lysis buffer to
 
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                the pellet and mix by vortex.</font><br />
 
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              <font size="1">7.- Add 150 uL of protein precipitation solution.</font><br />
 
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              <font size="1">8.- Centrifuge at 11,000 rpm during 5 min; then,
 
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                transfer the supernatant to a new microtube.</font><br />
 
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              <font size="1">9.- Add 700 uL of cold isopropanol and mix by
 
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                inverting until you see a white formation within the tube.</font><br />
 
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              <font size="1">10.- Centrifuge at 11,000 rpm during 4 min and pour
 
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                carefully taking care of the pellet at the bottom.</font><br />
 
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              <font size="1">11.- Add 500 uL of cold ethanol 70% to wash and
 
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                then centrifuge at 11,000 rpm during 4 min.</font><br />
 
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              <font size="1">12.- Pour and let the ethanol evaporate at room
 
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                temperature.</font><br />
 
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              <font size="1"> 13.- Resuspend the DNA pellet with 12 uL of TE
 
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                buffer; incubate all night at 4 °C.</font><br />
 
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            </p>
 
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          </div>
 
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          <div>
 
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            <h1>Protocol 4</h1>
 
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            <p>Electrophoresis:<br />
 
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              <br />
 
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              <font size="1">1.- Prepare 1L of TAE 10X (48.4g of Tris base[ tris
 
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                (hidroxymethyl) amminomethane], 11.4 mL of glacial acetic acid
 
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                (17.4 M) and 3.7 g of EDTA then fill with deionized water to 1
 
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                L.</font><br />
 
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              <font size="1">2.- Prepare a 50 mL solution of 3% agarose using
 
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                TAE buffer (without using water).</font><br />
 
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              <font size="1">3.- Heat the gel in the microwave in intervals of
 
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                30 s, 20 s and 10 s until the agarose is completely dissolved,
 
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                taking care that it does not boil so much.</font><br />
 
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              <font size="1">4.- Let cool down the gel (55-60°C) and pour in a
 
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                base with the comb already on its place to form the charging
 
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                wells.</font><br />
 
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              <font size="1">5.- Wait until the gel solidifies.</font><br />
 
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              <font size="1">6.- Remove the comb and place the base with the gel
 
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                inside the electrophoretic chamber.</font><br />
 
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              <font size="1">7.- Add TAE buffer until the gel is covered.</font><br />
 
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              <font size="1">8.- Take 5 uL of each sample from the PCR and 1 uL
 
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                of charging gel in a new PCR tube, mix well.</font><br />
 
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              <font size="1">9.- Charge the samples inside the wells of the gel,
 
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                adding a weight marcker.</font><br />
 
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              <font size="1">10.- Close the electrophoretic chamber and run at
 
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                120 V during 30 min. Follow the displacement ofthe samples.</font><br />
 
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              <font size="1">11.- Take out the gel from the chamber very
 
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                carefully and place in the UV light to see the DNA strands.</font><br />
 
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            </p>
 
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          </div>
 
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          <div>
 
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            <h1>Protocol 5</h1>
 
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            <p>Competent cell transformation:<br />
 
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              <br />
 
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              1.- Place 1 uL of the plasmid in a microtube, gently add 100 uL of
 
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              your competent cells. Take another tube and place only 100 uL of
 
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              competent cells, so you have a control group.<br />
 
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              2.- Mix gently with a pipette and incubate during 30 min in ice.<br />
 
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              3.- Ab ruptly place your cells in 42°C dry bath during 2 min and
 
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              replace in ice whwn finished.<br />
 
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              4.- Add 900 uL of LB medium and incubate at 37 °C for 30 min.<br />
 
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              5.- Inoculate with 200 uL of your transformed cells in LB-Amp.<br />
 
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              6.- Incubate overnight and store at 4 °C of freeze in 15-25%
 
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              glycerol.<br />
 
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            </p>
 
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          </div>
 
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          <div> <img alt="" src='https://static.igem.org/mediawiki/2014hs/e/e4/LabArchives.jpg' width='400' height='300'  />
 
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            <h1>Lab archives// Mar 12, 2014 </h1>
 
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            <p>In order to grow our bacteria, we inoculated with 100 uL of E.
 
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              Coli DH5-alpha in 50 mL of LB medium, and 100 uL of E. Coli TOP in
 
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              50 mL of LB medium. We left both samples incubating overnight </p>
 
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            <h1>Lab archives// Mar 20, 2014 </h1>
 
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            <p>We inoculated E.Coli NEB10-beta in 50 ml of LB medium. </p>
 
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          </div>
 
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          <div>
 
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            <h1>Lab archives// Mar 27, 2014 </h1>
 
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            <p>We prepared the medium in which our bacteria will grow, for that
 
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              we dissolved solid LB with agarose, then we add it into 10 plates,
 
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              we waited a few minutes for it to solidify and finally we added
 
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              500 uL of our antibiotic that is chloranphenicol to each of those
 
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              plates. </p>
 
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            <h1>Lab archives// Apr 01, 2014 </h1>
 
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            <p>we transformed luciferaze from DNA to cells by incerting them
 
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              with change of temperature </p>
 
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            <h1>Lab archives// Apr 02, 2014 </h1>
 
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            <p>We made a litter of agar and 500 L of growth medium, and we
 
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              sterilized them along with crystal media disperssion balls </p>
 
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            <h1>Lab archives// Apr 03, 2014 </h1>
 
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            <p>Today we prepared buffers for home made mini preps and did a cell
 
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              re culture. </p>
 
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          </div>
 
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          <div>
 
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            <h1><font size="1">Lab archives// Apr 07, 2014 </font> </h1>
 
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            <p> <font size="1">We added 700 mL of water in a beaker and then we
 
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                prepared 23 grams of powdered agar that we added slowly while we
 
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                moved the beaker. This in order to help the dissolve the powder.
 
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                After that, we added other 300 mL to the solution and finally we
 
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                passed it to an Erlenmeyer matrass. Also we prepared 500 mL of
 
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                growth medium. First, we measured and added 5 grams of tryptone,
 
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                5 grams of yeast and 10 grams of sodium chloride. Those
 
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                ingredients were added slowly to a graduated cylinder with 350
 
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                mL of wáter while the flask was moved. After finishing the
 
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                addition we added other 150 mL of water. The solution was then
 
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                transfered to another Erlenmeyer flask. Both Erlenmeyer flasks
 
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                and a glass bottle with crystal media diperssion balls were
 
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                sterilized with temperature and pressure.</font> </p>
 
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            <h1><font size="1">Lab archives// Apr 02, 2014 </font> </h1>
 
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            <p><font size="1">300 ul of ampicillin at 100mg/ml chloramphenicol </font><br />
 
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              <font size="1"> 3 Plates with agar </font><br />
 
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              <font size="1">Promoters: BBa_K258005 (pO2), BBa_176500 (pFe)
 
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                BBa_J52008 (luc-3) </font> <br />
 
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              <font size="1">The plates were impregnated with ampicillin
 
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                promoting bacteria in the following manner </font><br />
 
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              <font size="1"> |pO2 | Plate | 150 ul Ampiciline | K258005 | </font><br />
 
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              <font size="1">
 
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                ------------------------------------------------------ </font><br />
 
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              <font size="1"> |pFe | Plate | 150 ul Ampiciline | 1765000 | </font><br />
 
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              <font size="1">
 
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                ------------------------------------------------------ </font><br />
 
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              <font size="1"> |Luc-3 | Plate | Chloranphenicol | J52008 | </font><br />
 
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            </p>
 
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            <p></p>
 
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          </div>
 
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          <div>
 
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            <h1><font size="1">Apr 08, 2014 </font></h1>
 
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            <p> <font size="1"> **/Today we realized the mini prep for the
 
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                oxygen promoter. /** </font><br />
 
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              <font size="1"> --500 ul of Ampiciline //this is for the oxygen
 
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                promoter </font><br />
 
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              <font size="1"> --50 ul of oxygen promoter </font><br />
 
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              <font size="1"> --45 ml of LB medium </font><br />
 
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              <font size="1"> --at room temperature 15 minutes at 1500 rpm in
 
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                the centrifuge. </font><br />
 
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              <font size="1"> --500 ul of chloramphenicol // this is for the red
 
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                liciferase </font><br />
 
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              <font size="1"> --50 ul of red luciferase </font><br />
 
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              <font size="1"> --45 ml of LB medium </font><br />
 
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              <font size="1"> --The new LB medium was prepared with //LB medium
 
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              </font><br />
 
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              <font size="1"> --10 g yeast </font><br />
 
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              <font size="1"> --5 g salt </font><br />
 
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              <font size="1"> --15 g Tryptone </font> <font size="1"> MINI
 
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                PREP First 400 ul of Genomic Lysis Buffer to the 40 ml overnight
 
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                [bacteriaand oxygen promoter] Then the mixture is transferred to
 
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                a Zyne-Spine Collumn in a Collection Tube. Centrifuge 10,000 rpm
 
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                /1 min Transfer the Zyno Spin Column to a new Collection Tube.
 
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                Add 200 micro liters of Pre Wash Buffer. Centrifuge 10,000 rpm
 
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                /1 min Add 500 uL of of g-DNA Wash buffer to the spin column.
 
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                Centrifuge 10,000 rpm /1 min Transfer the spin column to a clear
 
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                micro centrifuge tube. Add 50 uL of DNA Elution Buffer .
 
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                Incubate 2-5 minutes to at room temperature. Centrifuge at top
 
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                speed per 30 seconds. Then store at -80 °C </font></p>
 
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          </div>
 
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          <div>
 
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            <h1><font size="1"> Apr 22, 2014</font> </h1>
 
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            <p><font size="1"> We prepared 2 overnight cultures (45ml of LB
 
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                medium): GFP E0040 pSB1A2 with 100 uL of ampicillin. M-cherry
 
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                (RFP) BBa_J04450 pSB1C3 with 500 uL of chloramphenicol.</font></p>
 
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            <h1><font size="1"> Apr 24, 2014 </font></h1>
 
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            <p><font size="1"> 2 Transformations: Bacteria NEB 10 Beta, DH5alpha
 
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                2 Mini Preps: GFP, RFP //Transformation and preps:--
 
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                Centrifugation at 2500 rpm for 15 min.--1ul DNA in 100 uL of
 
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                bacterial culture (luciferase). --Filtering GFP, RFP bacteria.
 
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                --Added 400 ml of Lysis Buffer Genomyc each crop by columns.
 
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                --Centrifugation at 10,000 * g for 1 min. --Last column to
 
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                another tube and DNA Prewash + 200 uL Buffer. --Centrifuged at
 
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                10,000 * g for 1 min. --Add 500ul of DNA Wash Buffer,
 
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                centrifuged at 10,000 * g for 1 min. --Transfer to clean tube,
 
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                add 50ul of DNA Elution Buffer. --Incubate for 2-5 min at room
 
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                temperature, centrifuged at maximum speed for 30 sec.</font></p>
 
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            <h1><font size="1"> Apr 28, 2014 </font></h1>
 
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            <p><font size="1"> Today was prepared LB media. It also was done the
 
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                sterilization of 1 mL and 200 uL tips, water and ependorfs.
 
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                After the sterilization of the LB media was done, we cultivated
 
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                in it the bacteria carrying the oxygen promoter, RFP, and GFP.
 
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                Then we left the bacteria growing in the incubator at 37°C
 
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                overnight.</font></p>
 
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          </div>
 
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          <div>
 
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            <h1><font size="1"> May 05, 2014 </font></h1>
 
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            <p><font size="1"> Plating bacteria with GFP, RFP, promoter Oxygen
 
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                and iron promoter plasmids. Everyone in their respective
 
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                environments and specific antibiotics (ampicillin and
 
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                chloramphenicol). Tubes with bacteria was added and placed them
 
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                in LB medium incubator to grow more product if necessary.</font></p>
 
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            <h1><font size="1"> May 08, 2014 </font></h1>
 
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            <p><font size="1"> We did a centrifuge at 4800 rpm for 20 min for
 
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                the plasmids had been in overnight which had oxygen promoter,
 
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                GFP and RFP to rush after we separate the aqueous phase.</font></p>
 
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            <h1><font size="1"> May 19, 2014 </font></h1>
 
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            <p><font size="1"> We did inoculation of GFP, RFP and Oxigen Prmoter</font></p>
 
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            <h1><font size="1"> May 20, 2014 </font></h1>
 
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            <p><font size="1"> We started minipreps preparation of GFP, RFP and
 
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                Oxigen Promoter. We added 1.5 ml of culture to each Eppendorff
 
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                (4 for each promoter) and we centrifugated it at 13.2 rpm for 10
 
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                minutes, we did the same procedure 2 times. Also, we sterilized
 
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                two Erlenmeyer flasks and 40 Eppendorffs. </font></p>
 
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          </div>
 
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          <div>
 
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            <h1><font size="1"> May 20, 2014 //Mini prep preparation </font></h1>
 
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            <p><font size="1"> 1. We added 1.5 mL of the cultures to their
 
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                corresponding Eppendorffs and centrifugated them at 3400 rpm
 
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                during 10 minutes other three times.</font></p>
 
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            <p><font size="1">2. Once we had a pellet we discarded the
 
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                supernatant and we added 300 uL of TEG buffer (made of 100 mM of
 
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                Tris-HCl pH8, 2 mM EDTA and 20% glucose)</font></p>
 
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            <p><font size="1">3. We added 700 μL of NS solution (0.2 M NaOH, 1%
 
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                w/v SDs) and we mixed by inversion.</font></p>
 
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            <p><font size="1">4. We added 400 μL of 3 M sodium acetate pH 5.3,
 
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                and we mixed by inversion.</font></p>
 
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            <p><font size="1">5. We centrifugated the Eppendorffs at 3400 rpm
 
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                during 15 minutes.</font></p>
 
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            <p><font size="1">6. We transfered the supernatant to other
 
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                Eppendorffs and we added 50 uL of cold isopropanol and mixed by
 
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                inversion.</font></p>
 
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            <p><font size="1">7. We centrifugated the Eppendorffs at 3400 rpm
 
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                during 15 minutes.</font></p>
 
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            <p><font size="1">8. We discarded the supernatant and washed each
 
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                pellet with 70% ethanol and we let it dry for 15 minutes.</font></p>
 
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            <p><font size="1">9. We resuspended the pellet in 500 uL of TE
 
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                buffer and then we added 10 mg/mL RNAseA.</font></p>
 
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            <p><font size="1">11. We precipitated the DNA with 100 uL of ethanol
 
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                in each Eppendorff.</font></p>
 
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            <p><font size="1">12. We centrifugated for 1 minute at 3400 rpm.</font></p>
 
-
            <p><font size="1">13. We got rid of the supernatant.</font></p>
 
-
            <p><font size="1">14. Finally, we resuspended the pellet in 50 uL of
 
-
                TE buffer</font></p>
 
-
          </div>
 
-
          <div> <img alt="" src='https://static.igem.org/mediawiki/2014hs/f/f4/1%281%29.jpg' width='400' height='300' />
 
-
            <h1>UNAM procedures// Digestion</h1>
 
-
            <p>|BSA | 1 uL | </p>
 
-
            <p>|DNA | 2 uL | </p>
 
-
            <p>|Buffer (2) | 1 uL |</p>
 
-
            <p>|Buffer Xba I+Pstl | 3.3 uL |</p>
 
-
            <p>|fe Xba I+Spef | 3.3 uL | </p>
 
-
            <p>|H<sub>2</sub>0 | 5.4 uL | </p>
 
-
          </div>
 
-
          <div>
 
-
            <h1><font size="1">Gel agarose</font></h1>
 
-
            <p><font size="1"> 1 kb NEB Ampyciline -&gt; Pentneryl </font></p>
 
-
            <p><font size="1">| ---------------Bristol - Myers Squibb</font> </p>
 
-
            <p><font size="1">| ---------------1 gram/ 3 mL</font> </p>
 
-
            <p><font size="1">| ---------------inyectable</font></p>
 
-
            <p><font size="1">|<sub>p</sub>O <sub>2</sub> | 3.3 uL</font> </p>
 
-
            <p><font size="1">|1 kb marker 5.4 uL </font></p>
 
-
            <h1><font size="1">Homemade Mini prep Preparation </font></h1>
 
-
            <p><font size="1"> 1.- Overnight bacterial culture of 5 mL in
 
-
                falkons of 50 mL </font></p>
 
-
            <p><font size="1"> 2.- Centrifugate in an eppendor of 1.5 mL and
 
-
                throw supernadant</font></p>
 
-
            <p><font size="1"> 3.- Add 152 uL of Buffer 1 + RNAse (11
 
-
                micro-liters /1 buffer micro-liter ) **Buffer 20% Glucouse </font></p>
 
-
            <p><font size="1"> 4.- Mix via Buffer and add 150 uL of Buffer 2
 
-
                *Wait 4-5 minutes*</font> </p>
 
-
            <p><font size="1"> (1.5mL)Buffer 2 -&gt; 880 uL H<sub>2</sub>O,20uL
 
-
                NaOH 10 N </font></p>
 
-
            <p><font size="1"> ---------------------------------------100 uL SDS
 
-
                10%</font> </p>
 
-
            <p><font size="1"> 5.- Mix via inversion and add150 uL Buffer 3 </font>
 
-
            </p>
 
-
            <p><font size="1"> ------------------------------Sodium Acetate 3M
 
-
                pH 5</font> </p>
 
-
          </div>
 
-
          <div>
 
-
            <p><font size="1"> 6.-Centrifuge 5 minutes.</font> </p>
 
-
            <p><font size="1"> 7.-Take the supernadant to antoher tube. Add 1 mL
 
-
                ETOH Absolute </font></p>
 
-
            <p><font size="1"> 8.-Centrifuge by 5 minutes and decant </font> </p>
 
-
            <p><font size="1"> 9.-Wash with 500uL ETOH 70 %</font> </p>
 
-
            <p><font size="1"> 10.-Centrifugate 1 minute </font></p>
 
-
            <p><font size="1"> 11.-Decant and dry at 37 celsius centigrades (30
 
-
                minutes)</font> </p>
 
-
            <p><font size="1"> 12.-Resuspend in 15 uL of H<sub>2</sub>O </font></p>
 
-
            <h1><font size="1">Ligation</font></h1>
 
-
            <p><font size="1"> Inserto 7mL</font></p>
 
-
            <p><font size="1"> Plasmid 3mL</font> </p>
 
-
            <p><font size="1"> Buffer 10x 1.5mL </font> </p>
 
-
            <p><font size="1"> Ligase 0.5 mL </font></p>
 
-
            <p><font size="1"> H<sub>2</sub>O 3 mL </font> </p>
 
-
            <p><font size="1"> Total = 15 ml </font></p>
 
-
          </div>
 
-
          <div>
 
-
            <h1>E.Coli cells transformation. </h1>
 
-
            <p>To transform E.Coli cells with an exogen DNA, 200 uL of competent
 
-
              cells were taken they were mixed with 1-20 ug(Micrograms) of DNA
 
-
              that was incubated on ice for 30 minutes. This makes posible the
 
-
              absorbtion of the exogen DNA to the cells surface, after that, the
 
-
              cells are incubated at 42Celsius Degrees during 90 seconds, for
 
-
              then incubate them during 1 minute again in ice. THis termal shock
 
-
              makes posible the entrance of the plasmid to the inside of the
 
-
              cell. 600 uL of LB medium were added and then incubated during
 
-
              60-90 minutes at 37 Celisus Degres, to do posible the segregation
 
-
              of the exogenic plasmid during the celular division (3 to 5
 
-
              generations). Finally, Alicuote of 100-200 uL were cultivated by
 
-
              dimention in the LB agar medium, which ere incubated at 37 Celsius
 
-
              Degrees. After 15-20 hours of incuvation colonies could be
 
-
              observed</p>
 
-
          </div>
 
-
          <div>
 
-
            <h1>Ligations</h1>
 
-
            <p>1# Grow h mL in overnight</p>
 
-
            <p>2# Do minipreps</p>
 
-
            <p>3# EcoRI and PstI restrictions.</p>
 
-
            <p> --for Fe EcoRIand SpeI</p>
 
-
            <p>O<sub>2</sub>+ GFP ---1,2,3 - 931</p>
 
-
            <p>Fe+GTP ---4,5,6 -1,828</p>
 
-
            <p>O<sub>2</sub>+ RFP ---7,8,9,10,11,12,13 -890</p>
 
-
            <p>Fe+RFP ---14,15,16,17,18,19 -1,787</p>
 
-
            <br />
 
-
            <p>GFP -&gt; 761 bp EcoRI Po+I // 728 bp XbaI SpeI</p>
 
-
            <p>RFP -&gt; 714 bp Xba - SpeI // 747 bp EcoR - Ps+I</p>
 
-
            <br />
 
-
            <p>pFe -&gt; 1,067 bp EcoRI - SpeI </p>
 
-
            <p><sub>p</sub>O<sub>2</sub> -&gt; 178 bp EcoRi SpeI // 145 bp Xba -
 
-
              Ps+I </p>
 
-
          </div>
 
-
          <div>
 
-
            <p>O<sub>2</sub> + GFP 1/1 - 1/2 - 3/3</p>
 
-
            <p>FO <sub>2</sub> + GFP 4/1 - 5/2 - 6/3</p>
 
-
            <p>O<sub>2</sub> + RFP 7/1 - 8/2 - 9/3 - 10/4 - 11/5 - 12/6 - 12/13
 
-
            </p>
 
-
            <p>Fe + RFP 13/1 - 14/2 - 15*16/3 - 17/4 - 18/5 -19/6</p>
 
-
            <h1>Digestion</h1>
 
-
            <p>2 --- BSA --- 1uL</p>
 
-
            <p>4 --- DNA --- 2uL</p>
 
-
            <p>3 --- (M) Buffer // Enz (0.3 uL each one)--- 1uL</p>
 
-
            <p>5 --- EcoRI --- 0.3 uL</p>
 
-
            <p> --- PstI --- 0.3 uL</p>
 
-
            <p>1 --- H<sub>2</sub>O --- 5.4 uL</p>
 
-
            <p> Total == 10 uL rxn</p>
 
-
            <br />
 
-
            <p> GFP 761 bp</p>
 
-
            <p> RFP 742 bp</p>
 
-
          </div>
 
-
          <div>
 
-
            <p> pFe 1,067 bp </p>
 
-
            <p> pO<sub>2</sub> -15.5 bp</p>
 
-
            <br />
 
-
            <p> pO<sub>2</sub> + GFP 931 bp</p>
 
-
            <p> pFe + GFP 1,828 bp</p>
 
-
            <p> pO<sub>2</sub>+ RFP 890 bp</p>
 
-
            <p> pFe + RFP 1,787 bp</p>
 
-
            <br />
 
-
            <p> Gel 1 = 8/9/10/Lodder 1kb/11/12/13</p>
 
-
            <p> Gel 2 = 15/16/17/Lodder 116/18/19</p>
 
-
            <p> Gel 3 = 1/2/3/4/Lodder 1kb/5/6/7</p>
 
-
          </div>
 
-
        </div>
 
-
      </div>
 
-
    </div>
 
-
    <div>  <span class="reference"> </span> </div>
 
-
    <!-- The JavaScript -->
 
-
    <script type="text/javascript">
 
-
$(function() {
 
-
var $mybook = $('#mybook');
 
-
var $bttn_next = $('#next_page_button');
 
-
var $bttn_prev = $('#prev_page_button');
 
-
var $loading = $('#loading');
 
-
var $mybook_images = $mybook.find('img');
 
-
var cnt_images = $mybook_images.length;
 
-
var loaded = 0;
 
-
//preload all the images in the book,
 
-
//and then call the booklet plugin
 
-
 
-
$mybook_images.each(function(){
 
-
var $img = $(this);
 
-
var source = $img.attr('src');
 
-
$('<img/>').load(function(){
 
-
++loaded;
 
-
if(loaded == cnt_images){
 
-
$loading.hide();
 
-
$bttn_next.show();
 
-
$bttn_prev.show();
 
-
$mybook.show().booklet({
 
-
name:              null,                            // name of the booklet to display in the document title bar
 
-
width:              800,                            // container width
 
-
height:            500,                            // container height
 
-
speed:              600,                            // speed of the transition between pages
 
-
direction:          'LTR',                          // direction of the overall content organization, default LTR, left to right, can be RTL for languages which read right to left
 
-
startingPage:      0,                              // index of the first page to be displayed
 
-
easing:            'easeInOutQuad',                // easing method for complete transition
 
-
easeIn:            'easeInQuad',                    // easing method for first half of transition
 
-
easeOut:            'easeOutQuad',                  // easing method for second half of transition
 
-
 
-
closed:            true,                          // start with the book "closed", will add empty pages to beginning and end of book
 
-
closedFrontTitle:  null,                            // used with "closed", "menu" and "pageSelector", determines title of blank starting page
 
-
closedFrontChapter: null,                            // used with "closed", "menu" and "chapterSelector", determines chapter name of blank starting page
 
-
closedBackTitle:    null,                            // used with "closed", "menu" and "pageSelector", determines chapter name of blank ending page
 
-
closedBackChapter:  null,                            // used with "closed", "menu" and "chapterSelector", determines chapter name of blank ending page
 
-
covers:            false,                          // used with  "closed", makes first and last pages into covers, without page numbers (if enabled)
 
-
 
-
pagePadding:        10,                              // padding for each page wrapper
 
-
pageNumbers:        true,                            // display page numbers on each page
 
-
 
-
hovers:            false,                            // enables preview pageturn hover animation, shows a small preview of previous or next page on hover
 
-
overlays:          false,                            // enables navigation using a page sized overlay, when enabled links inside the content will not be clickable
 
-
tabs:              false,                          // adds tabs along the top of the pages
 
-
tabWidth:          60,                              // set the width of the tabs
 
-
tabHeight:          20,                              // set the height of the tabs
 
-
arrows:            false,                          // adds arrows overlayed over the book edges
 
-
cursor:            'pointer',                      // cursor css setting for side bar areas
 
-
 
-
hash:              false,                          // enables navigation using a hash string, ex: #/page/1 for page 1, will affect all booklets with 'hash' enabled
 
-
keyboard:          true,                            // enables navigation with arrow keys (left: previous, right: next)
 
-
next:              $bttn_next,          // selector for element to use as click trigger for next page
 
-
prev:              $bttn_prev,          // selector for element to use as click trigger for previous page
 
-
 
-
menu:              null,                            // selector for element to use as the menu area, required for 'pageSelector'
 
-
pageSelector:      false,                          // enables navigation with a dropdown menu of pages, requires 'menu'
 
-
chapterSelector:    false,                          // enables navigation with a dropdown menu of chapters, determined by the "rel" attribute, requires 'menu'
 
-
 
-
shadows:            true,                            // display shadows on page animations
 
-
shadowTopFwdWidth:  166,                            // shadow width for top forward anim
 
-
shadowTopBackWidth: 166,                            // shadow width for top back anim
 
-
shadowBtmWidth:    50,                              // shadow width for bottom shadow
 
-
 
-
before:            function(){},                    // callback invoked before each page turn animation
 
-
after:              function(){}                    // callback invoked after each page turn animation
 
-
});
 
-
Cufon.refresh();
 
-
}
 
-
}).attr('src',source);
 
-
});
 
-
 
-
});
 
-
        </script>
 
-
  </body>
 
-
</html>
 

Latest revision as of 03:20, 21 June 2014