Data notebook.html

From 2014hs.igem.org

(Difference between revisions)
(Created page with "<html> <head> <style> *{ margin:0; padding:0; } body{ background:#ccc url(../images/DNA.jpg) repeat top left; font-family: Arial, Helvetica, sans-serif; color:#444; font-si...")
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<html>
 
-
<head>
 
-
<style>
 
-
*{
 
-
margin:0;
 
-
padding:0;
 
-
}
 
-
body{
 
-
background:#ccc url(../images/DNA.jpg) repeat top left;
 
-
font-family: Arial, Helvetica, sans-serif;
 
-
color:#444;
 
-
font-size:12px;
 
-
color: #000;
 
-
}
 
-
h1{
 
-
color:#000000;
 
-
font-size:40px;
 
-
margin:20px 0px 0px 20px;
 
-
}
 
-
span.reference{
 
-
font-family:Arial;
 
-
display:block;
 
-
font-size:12px;
 
-
text-align:center;
 
-
margin-bottom:10px;
 
-
}
 
-
span.reference a{
 
-
color:#000;
 
-
text-transform:uppercase;
 
-
text-decoration:none;
 
-
margin:0px 20px;
 
-
}
 
-
span.reference a:hover{
 
-
color:#ddd;
 
-
}
 
-
/* Booklet jQuery Plugin Style*/
 
-
.booklet{
 
-
-moz-box-shadow:0px 0px 1px #fff;
 
-
-webkit-box-shadow:0px 0px 1px #fff;
 
-
box-shadow:0px 0px 1px #fff;
 
-
-moz-border-radius:10px;
 
-
-webkit-border-radius:10px;
 
-
border-radius:10px;
 
-
}
 
-
.booklet .b-wrap-left  {
 
-
background:#fff url(../images/left_bg.jpg) no-repeat top left;
 
-
-webkit-border-top-left-radius: 10px;
 
-
-webkit-border-bottom-left-radius: 10px;
 
-
-moz-border-radius-topleft:10px;
 
-
-moz-border-radius-bottomleft: 10px;
 
-
border-top-left-radius: 10px;
 
-
border-bottom-left-radius: 10px;
 
-
}
 
-
.booklet .b-wrap-right {
 
-
background:#efefef url(../images/right_bg.jpg) no-repeat top left;
 
-
-webkit-border-top-right-radius: 10px;
 
-
-webkit-border-bottom-right-radius: 10px;
 
-
-moz-border-radius-topright: 10px;
 
-
-moz-border-radius-bottomright: 10px;
 
-
border-top-right-radius: 10px;
 
-
border-bottom-right-radius: 10px;
 
-
}
 
-
.booklet .b-counter {
 
-
bottom:10px;
 
-
position:absolute;
 
-
display:block;
 
-
width:90%;
 
-
height:20px;
 
-
border-top:1px solid #ddd;
 
-
color:#222;
 
-
text-align:center;
 
-
font-size:12px;
 
-
padding:5px 0 0;
 
-
background:transparent;
 
-
-moz-box-shadow:0px -1px 1px #fff;
 
-
-webkit-box-shadow:0px -1px 1px #fff;
 
-
box-shadow:0px -1px 1px #fff;
 
-
opacity:0.8;
 
-
}
 
-
.book_wrapper{
 
-
margin:0 auto;
 
-
padding-top:50px;
 
-
width:905px;
 
-
height:540px;
 
-
position:relative;
 
-
background:transparent url(../images/bg.png) no-repeat 9px 27px;
 
-
}
 
-
.book_wrapper h1{
 
-
color:#13386a;
 
-
margin:5px 5px 5px 15px;
 
-
font-size:26px;
 
-
background:transparent url(../images/h1.png) no-repeat bottom left;
 
-
padding-bottom:7px;
 
-
}
 
-
.book_wrapper p{
 
-
font-size:16px;
 
-
margin:5px 5px 5px 15px;
 
-
}
 
-
.book_wrapper a.article,
 
-
.book_wrapper a.demo{
 
-
background:transparent url(../images/circle.png) no-repeat 50% 0px;
 
-
display:block;
 
-
width:95px;
 
-
height:41px;
 
-
text-decoration:none;
 
-
outline:none;
 
-
font-size:16px;
 
-
color:#555;
 
-
float:left;
 
-
line-height:41px;
 
-
padding-left:47px;
 
-
}
 
-
.book_wrapper a.demo{
 
-
margin-left:50px;
 
-
}
 
-
.book_wrapper a.article:hover,
 
-
.book_wrapper a.demo:hover{
 
-
background-position:50% -41px;
 
-
color:#13386a;
 
-
}
 
-
.book_wrapper img{
 
-
margin:10px 0px 5px 35px;
 
-
width:300px;
 
-
padding:4px;
 
-
border:1px solid #ddd;
 
-
-moz-box-shadow:1px 1px 1px #fff;
 
-
-webkit-box-shadow:1px 1px 1px #fff;
 
-
box-shadow:1px 1px 1px #fff;
 
-
}
 
-
.booklet .b-wrap-right img{
 
-
border:1px solid #E6E3C2;
 
-
}
 
-
a#next_page_button,
 
-
a#prev_page_button{
 
-
display:none;
 
-
position:absolute;
 
-
width:41px;
 
-
height:40px;
 
-
cursor:pointer;
 
-
margin-top:-20px;
 
-
top:50%;
 
-
background:transparent url(../images/buttons.png) no-repeat 0px -40px;
 
-
}
 
-
a#prev_page_button{
 
-
left:-30px;
 
-
}
 
-
a#next_page_button{
 
-
right:-30px;
 
-
background-position:-41px -40px;
 
-
}
 
-
a#next_page_button:hover{
 
-
background-position:-41px 0px;
 
-
}
 
-
a#prev_page_button:hover{
 
-
background-position:0px 0px;
 
-
}
 
-
.loading{
 
-
width:160px;
 
-
height:56px;
 
-
position: absolute;
 
-
top:50%;
 
-
margin-top:-28px;
 
-
right:135px;
 
-
line-height:56px;
 
-
color:#fff;
 
-
padding-left:60px;
 
-
font-size:15px;
 
-
background: #000 url(../images/ajax-loader.gif) no-repeat 10px 50%;
 
-
opacity: 0.7;
 
-
z-index:9999;
 
-
-moz-border-radius:20px;
 
-
-webkit-border-radius:20px;
 
-
border-radius:20px;
 
-
filter:progid:DXImageTransform.Microsoft.Alpha(opacity=70);
 
-
}
 
-
</style>
 
-
<script>
 
-
/*
 
-
* jQuery Booklet Plugin
 
-
* Copyright (c) 2010 W. Grauvogel (http://builtbywill.com/)
 
-
*
 
-
* Dual licensed under the MIT (http://www.opensource.org/licenses/mit-license.php)
 
-
* and GPL (http://www.opensource.org/licenses/gpl-license.php) licenses.
 
-
*
 
-
* Version : 1.1.0
 
-
*
 
-
* Originally based on the work of:
 
-
* 1) Charles Mangin (http://clickheredammit.com/pageflip/)
 
-
*/
 
-
;(function($) {
 
-
 
 
-
$.fn.booklet = function(options){
 
-
 
-
var o = $.extend({}, $.fn.booklet.defaults, options);
 
-
 
-
return $(this).each(function()
 
-
{
 
-
var command, config, obj, id, i, target;
 
-
 
-
//option type string - api call
 
-
if(typeof options == 'string')
 
-
{
 
-
//check if booklet has been initialized
 
-
if($(this).data('booklet')){
 
-
command = options.toLowerCase();
 
-
obj = $.fn.booklet.interfaces[$(this).data('id')];
 
-
 
-
if(command == 'next'){ obj.next() }
 
-
else if(command == 'prev'){ obj.prev() }
 
-
 
-
}
 
-
}
 
-
//option type number - api call
 
-
else if(typeof options == 'number')
 
-
{
 
-
//check if booklet has been initialized
 
-
if($(this).data('booklet')){
 
-
target = options;
 
-
obj = $.fn.booklet.interfaces[$(this).data('id')];
 
-
 
-
if(target % 2 != 0) {
 
-
target-= 1;
 
-
}
 
-
 
-
obj.gotoPage(target);
 
-
}
 
-
 
-
}
 
-
//else build new booklet
 
-
else
 
-
{
 
-
config = $.extend(true, {}, o);
 
-
 
-
// Determine ID (Reuse array slots if possible)
 
-
id = $.fn.booklet.interfaces.length;
 
-
for(i = 0; i < id; i++)
 
-
{
 
-
  if(typeof $.fn.booklet.interfaces[i] == 'undefined'){ id = i; break; }
 
-
}
 
-
 
-
// Instantiate the booklet
 
-
obj = new booklet($(this), config, id);
 
-
 
-
// Add API references
 
-
$.fn.booklet.interfaces[id] = obj;
 
-
}
 
-
});
 
-
}
 
-
 
-
function booklet(target, options, id){
 
-
 
-
///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
 
-
//VARS + STRUCTURE
 
-
///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
 
-
 
-
var self, opts, b, src,
 
-
hash, i, j, p, diff, busy, init, rhover, lhover,
 
-
titles = new Array(), chapters = new Array(),
 
-
pN, p0, p1, p2, p3, p4, pNwrap, p0wrap, p1wrap, p2wrap, p3wrap, p4wrap, wraps, sF, sB,
 
-
overlays, overlayN, overlayP, tabs, tabN, tabP, arrows, arrowN, arrowP, next, prev, ctrlsN, ctrlsP,
 
-
menu, chapter, dd, ddUL, ddH, ddLI, ddA, ddT, ddC, ddCUL, ddCH, ddCLI, ddCA, ddCT,
 
-
empty = '<div class="b-page-empty" title="" rel=""></div>', blank = '<div class="b-page-blank" title="" rel=""></div>'
 
-
;
 
-
 
-
busy        = false;
 
-
init        = false;
 
-
rhover = lhover = false;
 
-
self        = this;
 
-
self.options = options;
 
-
self.id      = id;
 
-
self.hash    = '';
 
-
opts        = self.options;
 
-
b            = target.addClass('booklet');
 
-
src          = b.children('.b-load');
 
-
 
-
//save page titles and chapter names, add page numbers
 
-
initPages();
 
-
 
-
//store data for api calls
 
-
b.data('booklet',true);
 
-
b.data('id', id);
 
-
b.data('total', src.children().length);
 
-
 
-
///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
 
-
// SETUP OPTIONS
 
-
///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
 
-
 
-
//set width + height
 
-
if(!opts.width){
 
-
opts.width = b.width();
 
-
}
 
-
if(!opts.height){
 
-
opts.height = b.height();
 
-
}
 
-
b.width(opts.width);
 
-
b.height(opts.height);
 
-
 
-
//save page sizes and other vars
 
-
opts.pWidth  = opts.width/2;
 
-
opts.pWidthN = '-'+(opts.width/2)+'px';
 
-
opts.pWidthH = opts.width/4;
 
-
opts.pHeight = opts.height;
 
-
opts.pTotal  = src.children().length;
 
-
opts.speedH  = opts.speed/2;
 
-
 
-
//set startingPage
 
-
if(opts.direction == 'LTR'){
 
-
opts.curr = 0;
 
-
}else if(opts.direction == 'RTL'){
 
-
opts.curr = opts.pTotal-2;
 
-
}
 
-
if(!isNaN(opts.startingPage) && opts.startingPage <= opts.pTotal && opts.startingPage > 0){
 
-
if((opts.startingPage % 2) != 0){opts.startingPage--};
 
-
opts.curr = opts.startingPage;
 
-
}
 
-
 
-
//set booklet opts.name
 
-
if(opts.name){
 
-
document.title = opts.name;
 
-
}else{
 
-
opts.name = document.title;
 
-
}
 
-
 
-
//save shadow widths for anim
 
-
if(opts.shadows){
 
-
opts.shadowTopFwdWidth  = '-'+opts.shadowTopFwdWidth+'px';
 
-
opts.shadowTopBackWidth = '-'+opts.shadowTopBackWidth+'px';
 
-
}
 
-
 
-
//setup menu
 
-
if(opts.menu){
 
-
menu = $(opts.menu).addClass('b-menu');
 
-
p = opts.curr;
 
-
//setup page selctor
 
-
if(opts.pageSelector){
 
-
//add selector
 
-
dd = $('<div class="b-selector b-selector-page"><span class="b-current">'+ (p+1) +' - '+ (p+2) +'</span></div>').appendTo(menu);
 
-
ddUL = $('<ul></ul>').appendTo(dd).empty().css('height','auto');
 
-
 
-
//loop through all pages
 
-
for(i=0; i < opts.pTotal; i+=2){
 
-
j = i;
 
-
//nums for normal view
 
-
nums = (j+1) +'-'+ (j+2);
 
-
if(opts.closed){
 
-
//nums for closed book
 
-
j--;
 
-
if(i==0){nums='1'}
 
-
else if(i==opts.pTotal-2){nums=opts.pTotal-2}
 
-
else {nums = (j+1) +'-'+ (j+2);}
 
-
//nums for closed book with covers
 
-
if(opts.covers){
 
-
j--;
 
-
if(i==0){nums=''}
 
-
else if(i==opts.pTotal-2){nums=''}
 
-
else {nums = (j+1) +'-'+ (j+2);}
 
-
}
 
-
}
 
-
//nums for RTL direction
 
-
if(opts.direction == 'RTL'){
 
-
nums = (Math.abs(j - opts.pTotal)-1) +' - '+ ((Math.abs(j - opts.pTotal)));
 
-
if(opts.closed){
 
-
if(i==opts.pTotal-2){nums='1'}
 
-
else if(i==0){nums=opts.pTotal-2}
 
-
else{nums = (Math.abs(j - opts.pTotal)-3) +' - '+ ((Math.abs(j - opts.pTotal)-2));}
 
-
 
-
if(opts.covers){
 
-
if(i==opts.pTotal-2){nums=''}
 
-
else if(i==0){nums=''}
 
-
else{nums = (Math.abs(j - opts.pTotal)-5) +' - '+ ((Math.abs(j - opts.pTotal)-4));}
 
-
}
 
-
}
 
-
dd.find('.b-current').text(nums);
 
-
ddLI = $('<li><a href="#/page/'+ (i+1) +'" id="selector-page-'+i+'"><span class="b-text">'+ titles[i+1] +'</span><span class="b-num">'+ nums +'</span></a></li>').prependTo(ddUL);
 
-
}else{
 
-
if(i==0){dd.find('.b-current').text(nums);}
 
-
ddLI = $('<li><a href="#/page/'+ (i+1) +'" id="selector-page-'+i+'"><span class="b-text">'+ titles[i] +'</span><span class="b-num">'+ nums +'</span></a></li>').appendTo(ddUL);
 
-
}
 
-
 
-
ddA = ddLI.find('a');
 
-
if(!opts.hash){
 
-
ddA.click(function(){
 
-
if(opts.direction == 'RTL'){dd.find('.b-current').text($(this).find('.b-num').text());}
 
-
ddT = parseInt($(this).attr('id').replace('selector-page-',''));
 
-
self.gotoPage(ddT);
 
-
return false;
 
-
});
 
-
}
 
-
}
 
-
 
-
//set height
 
-
ddH = ddUL.height();
 
-
ddUL.css({'height':0, 'padding-bottom':0});
 
-
 
-
//add hover effects
 
-
dd.unbind('hover').hover(function(){
 
-
ddUL.stop().animate({height:ddH, paddingBottom:10}, 500);
 
-
},function(){
 
-
ddUL.stop().animate({height:0, paddingBottom:0}, 500);
 
-
});
 
-
}
 
-
 
-
//setup chapter selctor
 
-
if(opts.chapterSelector){
 
-
 
-
chapter = chapters[opts.curr];
 
-
if(chapter == ""){ chapter = chapters[opts.curr+1]; }
 
-
 
-
ddC = $('<div class="b-selector b-selector-chapter"><span class="b-current">'+chapter+'</span></div>').appendTo(menu);
 
-
ddCUL = $('<ul></ul>').appendTo(ddC).empty().css('height','auto');
 
-
 
-
for(i=0; i < opts.pTotal; i+=1){
 
-
if(chapters[i] != "" && typeof chapters[i] != "undefined"){
 
-
if(opts.direction == 'RTL'){
 
-
j = i;
 
-
if(j % 2 != 0){j--;}
 
-
ddC.find('.b-current').text(chapters[i]);
 
-
ddCLI = $('<li><a href="#/page/'+ (j+1) +'" id="selector-page-'+(j)+'"><span class="b-text">'+ chapters[i] +'</span></a></li>').prependTo(ddCUL);
 
-
}else{
 
-
ddCLI = $('<li><a href="#/page/'+ (i+1) +'" id="selector-page-'+i+'"><span class="b-text">'+ chapters[i] +'</span></a></li>').appendTo(ddCUL);
 
-
}
 
-
ddCA = ddCLI.find('a');
 
-
if(!opts.hash){
 
-
ddCA.click(function(){
 
-
if(opts.direction == 'RTL'){ddC.find('.b-current').text($(this).find('.b-text').text());}
 
-
ddCT = parseInt($(this).attr('id').replace('selector-page-',''));
 
-
self.gotoPage(ddCT);
 
-
return false;
 
-
});
 
-
}
 
-
}
 
-
}
 
-
 
-
ddCH = ddCUL.height();
 
-
ddCUL.css({'height':0, 'padding-bottom':0});
 
-
 
-
ddC.unbind('hover').hover(function(){
 
-
ddCUL.stop().animate({height:ddCH, paddingBottom:10}, 500);
 
-
},function(){
 
-
ddCUL.stop().animate({height:0, paddingBottom:0}, 500);
 
-
});
 
-
}
 
-
}
 
-
 
-
///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
 
-
// API METHODS
 
-
///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
 
-
 
-
$.extend(self,
 
-
{
 
-
next : function(){
 
-
if(!busy){
 
-
self.gotoPage(opts.curr+2);
 
-
}
 
-
},
 
-
prev : function(){
 
-
if(!busy){
 
-
self.gotoPage(opts.curr-2);
 
-
}
 
-
},
 
-
gotoPage : function(num){
 
-
//moving forward (increasing number)
 
-
if(num > opts.curr && num < opts.pTotal && num >= 0 && !busy){
 
-
busy = true;
 
-
diff = num - opts.curr;
 
-
opts.curr = num;
 
-
opts.before.call(self, opts);
 
-
updatePager();
 
-
updateCtrls();
 
-
updateHash(opts.curr+1, opts);
 
-
initAnim(diff, true, sF);
 
-
 
-
//hide p2 as p3 moves across it
 
-
p2.stop().animate({width:0}, opts.speedH, opts.easeIn);
 
-
//animate p3 from right to left (left: movement, width: reveal slide, paddingLeft: shadow underneath)
 
-
//call setuppages at end of animation to reset pages
 
-
p3.stop().animate({left:opts.pWidthH, width:opts.pWidthH, paddingLeft: opts.shadowBtmWidth}, opts.speedH, opts.easeIn)
 
-
        .animate({left:0, width:opts.pWidth, paddingLeft:0}, opts.speedH);
 
-
p3wrap.animate({left:opts.shadowBtmWidth}, opts.speedH, opts.easeIn)
 
-
  .animate({left:0}, opts.speedH, opts.easeOut, function(){updateAfter()});
 
-
//moving backward (decreasing number)
 
-
}else if(num < opts.curr && num < opts.pTotal && num >= 0 && !busy){
 
-
busy = true;
 
-
diff = opts.curr - num;
 
-
opts.curr = num;
 
-
opts.before.call(self, opts);
 
-
updatePager();
 
-
updateCtrls();
 
-
updateHash(opts.curr+1, opts);
 
-
initAnim(diff, false, sB);
 
-
 
-
//hide p1 as p0 moves across it
 
-
p1.animate({left:opts.pWidth, width:0}, opts.speed, opts.easing);
 
-
p1wrap.animate({left:opts.pWidthN}, opts.speed, opts.easing);
 
-
//animate p0 from left to right (right: movement, width: reveal slide, paddingLeft: shadow underneath)
 
-
p0.animate({left:opts.pWidthH, width:opts.pWidthH}, opts.speedH, opts.easeIn)
 
-
  .animate({left:opts.pWidth, width:opts.pWidth}, opts.speedH, opts.easeOut);
 
-
//animate .wrapper content with p0 to keep content right aligned throughout
 
-
//call setuppages at end of animation to reset pages
 
-
p0wrap.animate({right:opts.shadowBtmWidth}, opts.speedH,opts. easeIn)
 
-
  .animate({right:0}, opts.speedH, opts.easeOut, function(){updateAfter()});
 
-
}
 
-
}
 
-
});
 
-
 
-
///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
 
-
// SETUP CONTROLS
 
-
///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
 
-
 
-
//add prev next user defined controls
 
-
if(opts.next){
 
-
next = $(opts.next);
 
-
next.click(function(e){e.preventDefault(); self.next();});
 
-
}
 
-
if(opts.prev){
 
-
prev = $(opts.prev);
 
-
prev.click(function(e){e.preventDefault(); self.prev();});
 
-
}
 
-
 
-
//add overlays
 
-
if(opts.overlays){
 
-
overlayP = $('<div class="b-overlay b-overlay-prev b-prev" title="Previous Page"></div>').appendTo(b);
 
-
overlayN = $('<div class="b-overlay b-overlay-next b-next" title="Next Page"></div>').appendTo(b);
 
-
overlays = b.find('.b-overlay');
 
-
 
-
if ($.browser.msie) {
 
-
overlays.css({'background':'#fff','filter':'progid:DXImageTransform.Microsoft.Alpha(opacity=0) !important'});
 
-
}
 
-
}
 
-
 
-
//add tabs
 
-
if(opts.tabs){
 
-
tabP = $('<div class="b-tab b-tab-prev b-prev" title="Previous Page">Previous</div>').appendTo(b);
 
-
tabN = $('<div class="b-tab b-tab-next b-next" title="Next Page">Next</div>').appendTo(b);
 
-
tabs = b.find('.b-tab');
 
-
 
-
if(opts.tabWidth){
 
-
tabs.width(opts.tabWidth);
 
-
}
 
-
if(opts.tabHeight){
 
-
tabs.height(opts.tabHeight);
 
-
}
 
-
 
-
tabs.css({'top': '-'+tabN.outerHeight()+'px'});
 
-
b.css({'marginTop': tabN.outerHeight()});
 
-
 
-
//update ctrls for RTL direction
 
-
if(opts.direction == 'RTL'){
 
-
tabN.html('Previous').attr('title','Previous Page');
 
-
tabP.html('Next').attr('title','Next Page');
 
-
}
 
-
}else{
 
-
b.css({'marginTop': 0});
 
-
}
 
-
 
-
//add arrows
 
-
if(opts.arrows){
 
-
arrowP = $('<div class="b-arrow b-arrow-prev b-prev" title="Previous Page"><div>Previous</div></div>').appendTo(b);
 
-
arrowN = $('<div class="b-arrow b-arrow-next b-next" title="Next Page"><div>Next</div></div>').appendTo(b);
 
-
arrows = b.find('.b-arrow');
 
-
 
-
//update ctrls for RTL direction
 
-
if(opts.direction == 'RTL'){
 
-
arrowN.html('<div>Previous</div>').attr('title','Previous Page');
 
-
arrowP.html('<div>Next</div>').attr('title','Next Page');
 
-
}
 
-
}
 
-
 
-
//save all "b-prev" and "b-next" controls
 
-
ctrlsN = b.find('.b-next');
 
-
ctrlsP = b.find('.b-prev');
 
-
 
-
//add click actions
 
-
ctrlsN.click(function(e){e.preventDefault(); self.next();});
 
-
ctrlsP.click(function(e){e.preventDefault(); self.prev();});
 
-
 
-
//add page hover animations
 
-
if(opts.hovers){
 
-
ctrlsN.hover(
 
-
function(){
 
-
if(!busy && opts.curr+2 <= opts.pTotal-2){
 
-
//animate
 
-
p2.stop().animate({'width':opts.pWidth-40}, 500, opts.easing);
 
-
p3.stop().animate({'left':opts.width-40, 'width':20, paddingLeft: 10}, 500, opts.easing);
 
-
rhover = true;
 
-
}
 
-
},
 
-
function(){
 
-
if(!busy && opts.curr+2 <= opts.pTotal-2){
 
-
p2.stop().animate({'width':opts.pWidth}, 500, opts.easing);
 
-
p3.stop().animate({'left':opts.width, 'width':0, paddingLeft: 0}, 500, opts.easing);
 
-
rhover = false;
 
-
}
 
-
}
 
-
);
 
-
ctrlsP.hover(
 
-
function(){
 
-
if(!busy && opts.curr-2 >= 0){
 
-
//animate
 
-
p1.stop().animate({left:10, width:opts.pWidth-10}, 400, opts.easing);
 
-
p1wrap.stop().animate({left:"-10px"}, 400, opts.easing);
 
-
p0.stop().animate({left:10, width:40}, 400, opts.easing);
 
-
p0wrap.stop().animate({right:10}, 400, opts.easing);
 
-
lhover = true;
 
-
}
 
-
},
 
-
function(){
 
-
if(!busy && opts.curr-2 >= 0){
 
-
p1.stop().animate({left:0, width:opts.pWidth}, 400, opts.easing);
 
-
p1wrap.stop().animate({left:0}, 400, opts.easing);
 
-
p0.stop().animate({left:0, width:0}, 400, opts.easing);
 
-
p0wrap.stop().animate({right:0}, 400, opts.easing);
 
-
lhover = false;
 
-
}
 
-
}
 
-
);
 
-
}
 
-
 
-
//arrow animations
 
-
if(opts.arrows){
 
-
if($.support.opacity){
 
-
ctrlsN.hover(
 
-
function(){arrowN.find('div').stop().fadeTo('fast', 1);},
 
-
function(){arrowN.find('div').stop().fadeTo('fast', 0);
 
-
});
 
-
ctrlsP.hover(
 
-
function(){arrowP.find('div').stop().fadeTo('fast', 1);},
 
-
function(){arrowP.find('div').stop().fadeTo('fast', 0);
 
-
});
 
-
}else{
 
-
ctrlsN.hover(
 
-
function(){arrowN.find('div').show();},
 
-
function(){arrowN.find('div').hide();
 
-
});
 
-
ctrlsP.hover(
 
-
function(){arrowP.find('div').show();},
 
-
function(){arrowP.find('div').hide();
 
-
});
 
-
}
 
-
}
 
-
 
-
//keyboard ctrls
 
-
if(opts.keyboard){
 
-
//keyboard ctrls
 
-
$(document).keyup(function(event){
 
-
if(event.keyCode == 37){self.prev();}
 
-
else if(event.keyCode == 39){self.next();}
 
-
});
 
-
}
 
-
 
-
//hash ctrls
 
-
if(opts.hash){
 
-
setupHash();
 
-
clearInterval();
 
-
setInterval(function(){pollHash()}, 250);
 
-
}
 
-
 
-
//first time setup
 
-
resetPages();
 
-
 
-
///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
 
-
//General Functions
 
-
///////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
 
-
 
-
function initPages(){
 
-
//fix for odd number of pages
 
-
if((src.children().length % 2) != 0){
 
-
//if book is closed and using covers, add page before back cover, else after last page
 
-
if(opts.closed && opts.covers){
 
-
src.children().last().before(blank);
 
-
}else{
 
-
src.children().last().after(blank);
 
-
}
 
-
}
 
-
 
-
//if closed book, add empty pages to start and end
 
-
if(opts.closed){
 
-
$(empty).attr({'title':opts.closedFrontTitle || "Beginning", 'rel':opts.closedFrontChapter || "Beginning of Book"}).prependTo(src);
 
-
src.children().last().attr({'title':opts.closedBackTitle || "End", 'rel':opts.closedBackChapter || "End of Book"});
 
-
src.append(empty);
 
-
}
 
-
 
-
if(opts.direction == 'LTR'){
 
-
j = 0;
 
-
}else{
 
-
j = src.children().length;
 
-
if(opts.closed){j-=2;}
 
-
if(opts.covers){j-=2;}
 
-
$(src.children().get().reverse()).each(function(){
 
-
$(this).appendTo(src);
 
-
});
 
-
}
 
-
 
-
//save titles and chapters
 
-
src.children().each(function(i){
 
-
//save chapter title
 
-
if($(this).attr('rel')){
 
-
chapters[i] = $(this).attr('rel');
 
-
}else{
 
-
chapters[i] = "";
 
-
}
 
-
//save page title
 
-
titles[i] = $(this).attr('title');
 
-
 
-
//give content the correct wrapper and page wrapper
 
-
if($(this).hasClass('b-page-empty')){
 
-
$(this).wrap('<div class="b-page"><div class="b-wrap"></div></div>');
 
-
}else if(opts.closed && opts.covers && (i == 1 || i == src.children().length-2)){
 
-
$(this).wrap('<div class="b-page"><div class="b-wrap b-page-cover"></div></div>');
 
-
}else if(i % 2 != 0){
 
-
$(this).wrap('<div class="b-page"><div class="b-wrap b-wrap-right"></div></div>');
 
-
}else{
 
-
$(this).wrap('<div class="b-page"><div class="b-wrap b-wrap-left"></div></div>');
 
-
}
 
-
 
-
$(this).parents('.b-page').addClass('b-page-'+i);
 
-
 
-
//add page numbers
 
-
if(opts.pageNumbers && !$(this).hasClass('b-page-empty') && (!opts.closed || (opts.closed && !opts.covers) || (opts.closed && opts.covers && i != 1 && i != src.children().length-2))){
 
-
if(opts.direction == 'LTR'){j++;}
 
-
$(this).parent().append('<div class="b-counter">'+(j)+'</div>');
 
-
if(opts.direction == 'RTL'){j--;}
 
-
}
 
-
});
 
-
 
-
}
 
-
 
-
function resetPages(){
 
-
//reset all content
 
-
b.find('.b-page').removeClass('b-pN b-p0 b-p1 b-p2 b-p3 b-p4').hide();
 
-
if(init){
 
-
j = opts.pTotal-1;
 
-
for(i=0;i<opts.pTotal;i++){
 
-
b.find('.b-page-'+i).detach().appendTo(src);
 
-
}
 
-
}
 
-
 
-
//add page classes
 
-
if(opts.curr-2 >= 0){
 
-
b.find('.b-page-'+(opts.curr-2)).addClass('b-pN').show();
 
-
b.find('.b-page-'+(opts.curr-1)).addClass('b-p0').show();
 
-
}
 
-
b.find('.b-page-'+(opts.curr)).addClass('b-p1').show();
 
-
b.find('.b-page-'+(opts.curr+1)).addClass('b-p2').show();
 
-
if(opts.curr+3 <= opts.pTotal){
 
-
b.find('.b-page-'+(opts.curr+2)).addClass('b-p3').show();
 
-
b.find('.b-page-'+(opts.curr+3)).addClass('b-p4').show();
 
-
}
 
-
 
-
//save structure elems to vars
 
-
pN    = b.find('.b-pN');
 
-
p0    = b.find('.b-p0');
 
-
p1    = b.find('.b-p1');
 
-
p2    = b.find('.b-p2');
 
-
p3    = b.find('.b-p3');
 
-
p4    = b.find('.b-p4');
 
-
pNwrap = b.find('.b-pN .b-wrap');
 
-
p0wrap = b.find('.b-p0 .b-wrap');
 
-
p1wrap = b.find('.b-p1 .b-wrap');
 
-
p2wrap = b.find('.b-p2 .b-wrap');
 
-
p3wrap = b.find('.b-p3 .b-wrap');
 
-
p4wrap = b.find('.b-p4 .b-wrap');
 
-
wraps  = b.find('.b-wrap');
 
-
 
-
//update css
 
-
wraps.attr('style','');
 
-
wraps.css({'width':opts.pWidth-(opts.pagePadding*2), 'height':opts.pHeight-(opts.pagePadding*2), 'padding': opts.pagePadding});
 
-
p1.css({'left':0,'width':opts.pWidth, 'height':opts.pHeight});
 
-
p2.css({'left':opts.pWidth, 'width':opts.pWidth, 'opacity':1, 'height':opts.pHeight});
 
-
pN.css({'left':0, 'width':opts.pWidth, 'height':opts.pHeight});
 
-
p0.css({'left':0, 'width':0, 'height':opts.pHeight});
 
-
p3.stop().css({'left':opts.pWidth*2, 'width':0, 'height':opts.pHeight, paddingLeft:0});
 
-
p3wrap.stop().css({'left':0});
 
-
p4.css({'left':opts.pWidth, 'width':opts.pWidth, 'height':opts.pHeight});
 
-
 
-
//update page order for animations
 
-
if(opts.curr+3 <= opts.pTotal){
 
-
p3.after(p0.detach());
 
-
p1.after(p4.detach());
 
-
}else{
 
-
p0.detach().appendTo(src);
 
-
}
 
-
init = true;
 
-
 
-
//add shadows
 
-
sF = sB = null;
 
-
b.find('.b-shadow-b, .b-shadow-f').remove();
 
-
if(opts.shadows){
 
-
sF = $('<div class="b-shadow-f"></div>').appendTo(p3).css({'right':0,'width':opts.pWidth, 'height':opts.pHeight});
 
-
sB = $('<div class="b-shadow-b"></div>').appendTo(p0).css({'left':0,'width':opts.pWidth, 'height':opts.pHeight});
 
-
}
 
-
}
 
-
 
-
function initAnim(diff, inc, shadow){
 
-
//setup content
 
-
if(inc && diff > 2){
 
-
b.find('.b-p3, .b-p4').removeClass('b-p3 b-p4').hide();
 
-
b.find('.b-page-'+opts.curr).addClass('b-p3').show().stop().css({'left':opts.pWidth*2, 'width':0, 'height':opts.pHeight, paddingLeft:0});
 
-
b.find('.b-page-'+(opts.curr+1)).addClass('b-p4').show().css({'left':opts.pWidth, 'width':opts.pWidth, 'height':opts.pHeight});
 
-
b.find('.b-page-'+opts.curr+' .b-wrap').show().css({'width':opts.pWidth-(opts.pagePadding*2), 'height':opts.pHeight-(opts.pagePadding*2), 'padding': opts.pagePadding});
 
-
b.find('.b-page-'+(opts.curr+1)+' .b-wrap').show().css({'width':opts.pWidth-(opts.pagePadding*2), 'height':opts.pHeight-(opts.pagePadding*2), 'padding': opts.pagePadding});
 
-
 
-
p3    = b.find('.b-p3');
 
-
p4    = b.find('.b-p4');
 
-
p3wrap = b.find('.b-p3 .b-wrap');
 
-
p4wrap = b.find('.b-p4 .b-wrap');
 
-
 
-
if(rhover){
 
-
p3.css({'left':opts.width-40, 'width':20, 'padding-left': 10});
 
-
}
 
-
 
-
shadow.appendTo(p3);
 
-
 
-
p1.after(p4.detach());
 
-
p2.after(p3.detach());
 
-
}else if(!inc && diff > 2){
 
-
b.find('.b-pN, .b-p0').removeClass('b-pN b-p0').hide();
 
-
b.find('.b-page-'+opts.curr).addClass('b-pN').show().css({'left':0, 'width':opts.pWidth, 'height':opts.pHeight});
 
-
b.find('.b-page-'+(opts.curr+1)).addClass('b-p0').show().css({'left':0, 'width':0, 'height':opts.pHeight});
 
-
b.find('.b-page-'+opts.curr+' .b-wrap').show().css({'width':opts.pWidth-(opts.pagePadding*2), 'height':opts.pHeight-(opts.pagePadding*2), 'padding': opts.pagePadding});
 
-
b.find('.b-page-'+(opts.curr+1)+' .b-wrap').show().css({'width':opts.pWidth-(opts.pagePadding*2), 'height':opts.pHeight-(opts.pagePadding*2), 'padding': opts.pagePadding});
 
-
 
-
pN    = b.find('.b-pN');
 
-
p0    = b.find('.b-p0');
 
-
pNwrap = b.find('.b-pN .b-wrap');
 
-
p0wrap = b.find('.b-p0 .b-wrap');
 
-
 
-
if(lhover){
 
-
p0.css({left:10, width:40});
 
-
p0wrap.css({right:10});
 
-
}
 
-
 
-
shadow.appendTo(p0);
 
-
 
-
p0.detach().appendTo(src);
 
-
}
 
-
 
-
//updates if moving to start and end of book
 
-
if(opts.closed){
 
-
if(!inc && opts.curr == 0){
 
-
pN.hide();
 
-
}else if(!inc){
 
-
pN.show();
 
-
}
 
-
if(inc && opts.curr >= opts.pTotal-2){
 
-
p4.hide();
 
-
}else if(inc){
 
-
p4.show();
 
-
}
 
-
}
 
-
//init shadows
 
-
if(opts.shadows){
 
-
//check for opacity support -> animate shadow overlay on moving slide
 
-
if($.support.opacity){
 
-
shadow.animate({opacity:1}, opts.speedH, opts.easeIn)
 
-
  .animate({opacity:0}, opts.speedH, opts.easeOut);
 
-
}else{
 
-
if(inc){
 
-
shadow.animate({right:opts.shadowTopFwdWidth}, opts.speed, opts.easeIn);
 
-
}else{
 
-
shadow.animate({left:opts.shadowTopBackWidth}, opts.speed, opts.easeIn);
 
-
}
 
-
}
 
-
}
 
-
}
 
-
 
-
function updateAfter(){
 
-
resetPages();
 
-
updatePager();
 
-
updateCtrls();
 
-
opts.after.call(self, opts);
 
-
busy = false;
 
-
}
 
-
 
-
function updateCtrls(){
 
-
//update ctrls, cursors and visibility
 
-
if(opts.overlays || opts.tabs || opts.arrows){
 
-
if(opts.curr < opts.pTotal-2){
 
-
ctrlsN.fadeIn('fast').css('cursor',opts.cursor);
 
-
}else{
 
-
ctrlsN.fadeOut('fast').css('cursor','default');
 
-
}
 
-
if(opts.curr >= 2 && opts.curr != 0){         
 
-
ctrlsP.fadeIn('fast').css('cursor',opts.cursor);
 
-
}else{
 
-
ctrlsP.fadeOut('fast').css('cursor','default');
 
-
}
 
-
}
 
-
}
 
-
 
-
function updatePager(){
 
-
if(opts.pageSelector){
 
-
if(opts.direction == 'RTL'){
 
-
nums = (Math.abs(opts.curr - opts.pTotal)-1) +' - '+ ((Math.abs(opts.curr - opts.pTotal)));
 
-
if(opts.closed){
 
-
if(opts.curr==opts.pTotal-2){nums='1'}
 
-
else if(opts.curr==0){nums=opts.pTotal-2}
 
-
else{nums = (Math.abs(opts.curr - opts.pTotal)-2) +' - '+ ((Math.abs(opts.curr - opts.pTotal)-1));}
 
-
 
-
if(opts.covers){
 
-
if(opts.curr==opts.pTotal-2){nums=''}
 
-
else if(opts.curr==0){nums=''}
 
-
else{nums = (Math.abs(opts.curr - opts.pTotal)-3) +' - '+ ((Math.abs(opts.curr - opts.pTotal)-2));}
 
-
}
 
-
}
 
-
$(opts.menu+' .b-selector-page .b-current').text(nums);
 
-
}else{
 
-
nums = (opts.curr+1) +' - '+ (opts.curr+2);
 
-
if(opts.closed){
 
-
if(opts.curr==0){nums='1'}
 
-
else if(opts.curr==opts.pTotal-2){nums=opts.pTotal-2}
 
-
else {nums = (opts.curr) +'-'+ (opts.curr+1);}
 
-
 
-
if(opts.covers){
 
-
if(opts.curr==0){nums=''}
 
-
else if(opts.curr==opts.pTotal-2){nums=''}
 
-
else {nums = (opts.curr-1) +'-'+ (opts.curr);}
 
-
}
 
-
}
 
-
$(opts.menu+' .b-selector-page .b-current').text(nums);
 
-
}
 
-
}
 
-
if(opts.chapterSelector){
 
-
if(chapters[opts.curr]!=""){
 
-
$(opts.menu+' .b-selector-chapter .b-current').text(chapters[opts.curr]);
 
-
}else if(chapters[opts.curr+1]!=""){
 
-
$(opts.menu+' .b-selector-chapter .b-current').text(chapters[opts.curr+1]);
 
-
}
 
-
 
-
if(opts.direction == 'RTL' && chapters[opts.curr+1]!=""){
 
-
$(opts.menu+' .b-selector-chapter .b-current').text(chapters[opts.curr+1]);
 
-
}else if(chapters[opts.curr]!=""){
 
-
$(opts.menu+' .b-selector-chapter .b-current').text(chapters[opts.curr]);
 
-
}
 
-
}
 
-
}
 
-
 
-
function setupHash(){
 
-
hash = getHashNum();
 
-
 
-
if(!isNaN(hash) && hash <= opts.pTotal-1 && hash >= 0 && hash != ''){
 
-
if((hash % 2) != 0){
 
-
hash--;
 
-
}
 
-
opts.curr = hash;
 
-
}else{
 
-
updateHash(opts.curr+1, opts);
 
-
}
 
-
 
-
self.hash = hash;
 
-
}
 
-
 
-
function pollHash(){
 
-
hash = getHashNum();
 
-
//check page num
 
-
if(!isNaN(hash) && hash <= opts.pTotal-1 && hash >= 0){
 
-
if(hash != opts.curr && hash.toString()!=self.hash){
 
-
if((hash % 2) != 0){hash--};
 
-
 
-
document.title = opts.name + " - Page "+ (hash+1);
 
-
 
-
if(!busy){
 
-
self.gotoPage(hash);
 
-
self.hash = hash;
 
-
}
 
-
}
 
-
}
 
-
}
 
-
 
-
//get page number from hash tag, last element
 
-
function getHashNum(){
 
-
var hash = window.location.hash.split('/');
 
-
if(hash.length > 1){
 
-
return parseInt(hash[2])-1;
 
-
}else{
 
-
return '';
 
-
}
 
-
}
 
-
 
-
//set the hash
 
-
function updateHash(hash, opts){
 
-
if(opts.hash){
 
-
window.location.hash = "/page/" + hash;
 
-
}
 
-
}
 
-
 
-
}
 
-
 
-
//define empty array to hold API references
 
-
$.fn.booklet.interfaces = [];
 
-
 
-
//define default options
 
-
$.fn.booklet.defaults = {
 
-
name:              null,                            // name of the booklet to display in the document title bar
 
-
width:              600,                            // container width
 
-
height:            400,                            // container height
 
-
speed:              1000,                            // speed of the transition between pages
 
-
direction:          'LTR',                          // direction of the overall content organization, default LTR, left to right, can be RTL for languages which read right to left
 
-
startingPage:      0,                              // index of the first page to be displayed
 
-
easing:            'easeInOutQuad',                // easing method for complete transition
 
-
easeIn:            'easeInQuad',                    // easing method for first half of transition
 
-
easeOut:            'easeOutQuad',                  // easing method for second half of transition
 
-
 
-
closed:            false,                          // start with the book "closed", will add empty pages to beginning and end of book
 
-
closedFrontTitle:  null,                            // used with "closed", "menu" and "pageSelector", determines title of blank starting page
 
-
closedFrontChapter: null,                            // used with "closed", "menu" and "chapterSelector", determines chapter name of blank starting page
 
-
closedBackTitle:    null,                            // used with "closed", "menu" and "pageSelector", determines chapter name of blank ending page
 
-
closedBackChapter:  null,                            // used with "closed", "menu" and "chapterSelector", determines chapter name of blank ending page
 
-
covers:            false,                          // used with  "closed", makes first and last pages into covers, without page numbers (if enabled)
 
-
 
-
pagePadding:        10,                              // padding for each page wrapper
 
-
pageNumbers:        true,                            // display page numbers on each page
 
-
 
-
hovers:            true,                            // enables preview pageturn hover animation, shows a small preview of previous or next page on hover
 
-
overlays:          true,                            // enables navigation using a page sized overlay, when enabled links inside the content will not be clickable
 
-
tabs:              false,                          // adds tabs along the top of the pages
 
-
tabWidth:          60,                              // set the width of the tabs
 
-
tabHeight:          20,                              // set the height of the tabs
 
-
arrows:            false,                          // adds arrows overlayed over the book edges
 
-
cursor:            'pointer',                      // cursor css setting for side bar areas
 
-
 
-
hash:              false,                          // enables navigation using a hash string, ex: #/page/1 for page 1, will affect all booklets with 'hash' enabled
 
-
keyboard:          true,                            // enables navigation with arrow keys (left: previous, right: next)
 
-
next:              null,                            // selector for element to use as click trigger for next page
 
-
prev:              null,                            // selector for element to use as click trigger for previous page
 
-
 
-
menu:              null,                            // selector for element to use as the menu area, required for 'pageSelector'
 
-
pageSelector:      false,                          // enables navigation with a dropdown menu of pages, requires 'menu'
 
-
chapterSelector:    false,                          // enables navigation with a dropdown menu of chapters, determined by the "rel" attribute, requires 'menu'
 
-
 
-
shadows:            true,                            // display shadows on page animations
 
-
shadowTopFwdWidth:  166,                            // shadow width for top forward anim
 
-
shadowTopBackWidth: 166,                            // shadow width for top back anim
 
-
shadowBtmWidth:    50,                              // shadow width for bottom shadow
 
-
 
-
before:            function(){},                    // callback invoked before each page turn animation
 
-
after:              function(){}                    // callback invoked after each page turn animation
 
-
}
 
-
 
-
})(jQuery);
 
-
/*
 
-
* jQuery Booklet Plugin
 
-
* Copyright (c) 2010 W. Grauvogel (http://builtbywill.com/)
 
-
*
 
-
* Dual licensed under the MIT (http://www.opensource.org/licenses/mit-license.php)
 
-
* and GPL (http://www.opensource.org/licenses/gpl-license.php) licenses.
 
-
*
 
-
* Version : 1.1.0
 
-
*
 
-
* Originally based on the work of:
 
-
* 1) Charles Mangin (http://clickheredammit.com/pageflip/)
 
-
*/
 
-
(function(b){b.fn.booklet=function(c){var d=b.extend({},b.fn.booklet.defaults,c);return b(this).each(function(){var j,e,h,k,f,g;if(typeof c=="string"){if(b(this).data("booklet")){j=c.toLowerCase();h=b.fn.booklet.interfaces[b(this).data("id")];if(j=="next"){h.next()}else{if(j=="prev"){h.prev()}}}}else{if(typeof c=="number"){if(b(this).data("booklet")){g=c;h=b.fn.booklet.interfaces[b(this).data("id")];if(g%2!=0){g-=1}h.gotoPage(g)}}else{e=b.extend(true,{},d);k=b.fn.booklet.interfaces.length;for(f=0;f<k;f++){if(typeof b.fn.booklet.interfaces[f]=="undefined"){k=f;break}}h=new a(b(this),e,k);b.fn.booklet.interfaces[k]=h}}})};function a(ac,ae,G){var ao,av,S,A,O,L,I,E,o,ad,ah,C,N,aw=new Array(),K=new Array(),F,ab,Z,Y,W,U,ay,ag,J,s,c,aq,am,ax,az,T,al,ak,R,aj,ai,m,D,B,af,y,e,d,l,X,q,v,r,an,w,k,u,P,n,au,t,g,ap='<div class="b-page-empty" title="" rel=""></div>',at='<div class="b-page-blank" title="" 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class="b-num">'+nums+"</span></a></li>").prependTo(v)}else{if(L==0){q.find(".b-current").text(nums)}an=b('<li><a href="#/page/'+(L+1)+'" id="selector-page-'+L+'"><span class="b-text">'+aw[L]+'</span><span class="b-num">'+nums+"</span></a></li>").appendTo(v)}w=an.find("a");if(!av.hash){w.click(function(){if(av.direction=="RTL"){q.find(".b-current").text(b(this).find(".b-num").text())}k=parseInt(b(this).attr("id").replace("selector-page-",""));ao.gotoPage(k);return false})}}r=v.height();v.css({height:0,"padding-bottom":0});q.unbind("hover").hover(function(){v.stop().animate({height:r,paddingBottom:10},500)},function(){v.stop().animate({height:0,paddingBottom:0},500)})}if(av.chapterSelector){X=K[av.curr];if(X==""){X=K[av.curr+1]}u=b('<div class="b-selector b-selector-chapter"><span class="b-current">'+X+"</span></div>").appendTo(l);P=b("<ul></ul>").appendTo(u).empty().css("height","auto");for(L=0;L<av.pTotal;L+=1){if(K[L]!=""&&typeof K[L]!="undefined"){if(av.direction=="RTL"){I=L;if(I%2!=0){I--}u.find(".b-current").text(K[L]);au=b('<li><a href="#/page/'+(I+1)+'" id="selector-page-'+(I)+'"><span class="b-text">'+K[L]+"</span></a></li>").prependTo(P)}else{au=b('<li><a href="#/page/'+(L+1)+'" id="selector-page-'+L+'"><span class="b-text">'+K[L]+"</span></a></li>").appendTo(P)}t=au.find("a");if(!av.hash){t.click(function(){if(av.direction=="RTL"){u.find(".b-current").text(b(this).find(".b-text").text())}g=parseInt(b(this).attr("id").replace("selector-page-",""));ao.gotoPage(g);return false})}}}n=P.height();P.css({height:0,"padding-bottom":0});u.unbind("hover").hover(function(){P.stop().animate({height:n,paddingBottom:10},500)},function(){P.stop().animate({height:0,paddingBottom:0},500)})}}b.extend(ao,{next:function(){if(!ad){ao.gotoPage(av.curr+2)}},prev:function(){if(!ad){ao.gotoPage(av.curr-2)}},gotoPage:function(i){if(i>av.curr&&i<av.pTotal&&i>=0&&!ad){ad=true;o=i-av.curr;av.curr=i;av.before.call(ao,av);ar();z();M(av.curr+1,av);Q(o,true,ax);Y.stop().animate({width:0},av.speedH,av.easeIn);W.stop().animate({left:av.pWidthH,width:av.pWidthH,paddingLeft:av.shadowBtmWidth},av.speedH,av.easeIn).animate({left:0,width:av.pWidth,paddingLeft:0},av.speedH);c.animate({left:av.shadowBtmWidth},av.speedH,av.easeIn).animate({left:0},av.speedH,av.easeOut,function(){H()})}else{if(i<av.curr&&i<av.pTotal&&i>=0&&!ad){ad=true;o=av.curr-i;av.curr=i;av.before.call(ao,av);ar();z();M(av.curr+1,av);Q(o,false,az);Z.animate({left:av.pWidth,width:0},av.speed,av.easing);J.animate({left:av.pWidthN},av.speed,av.easing);ab.animate({left:av.pWidthH,width:av.pWidthH},av.speedH,av.easeIn).animate({left:av.pWidth,width:av.pWidth},av.speedH,av.easeOut);ag.animate({right:av.shadowBtmWidth},av.speedH,av.easeIn).animate({right:0},av.speedH,av.easeOut,function(){H()})}}}});if(av.next){af=b(av.next);af.click(function(i){i.preventDefault();ao.next()})}if(av.prev){y=b(av.prev);y.click(function(i){i.preventDefault();ao.prev()})}if(av.overlays){ak=b('<div 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.b-wrap");am=S.find(".b-wrap");am.attr("style","");am.css({width:av.pWidth-(av.pagePadding*2),height:av.pHeight-(av.pagePadding*2),padding:av.pagePadding});Z.css({left:0,width:av.pWidth,height:av.pHeight});Y.css({left:av.pWidth,width:av.pWidth,opacity:1,height:av.pHeight});F.css({left:0,width:av.pWidth,height:av.pHeight});ab.css({left:0,width:0,height:av.pHeight});W.stop().css({left:av.pWidth*2,width:0,height:av.pHeight,paddingLeft:0});c.stop().css({left:0});U.css({left:av.pWidth,width:av.pWidth,height:av.pHeight});if(av.curr+3<=av.pTotal){W.after(ab.detach());Z.after(U.detach())}else{ab.detach().appendTo(A)}ah=true;ax=az=null;S.find(".b-shadow-b, .b-shadow-f").remove();if(av.shadows){ax=b('<div class="b-shadow-f"></div>').appendTo(W).css({right:0,width:av.pWidth,height:av.pHeight});az=b('<div class="b-shadow-b"></div>').appendTo(ab).css({left:0,width:av.pWidth,height:av.pHeight})}}function Q(j,i,p){if(i&&j>2){S.find(".b-p3, .b-p4").removeClass("b-p3 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"+((Math.abs(av.curr-av.pTotal)));if(av.closed){if(av.curr==av.pTotal-2){nums="1"}else{if(av.curr==0){nums=av.pTotal-2}else{nums=(Math.abs(av.curr-av.pTotal)-2)+" - "+((Math.abs(av.curr-av.pTotal)-1))}}if(av.covers){if(av.curr==av.pTotal-2){nums=""}else{if(av.curr==0){nums=""}else{nums=(Math.abs(av.curr-av.pTotal)-3)+" - "+((Math.abs(av.curr-av.pTotal)-2))}}}}b(av.menu+" .b-selector-page .b-current").text(nums)}else{nums=(av.curr+1)+" - "+(av.curr+2);if(av.closed){if(av.curr==0){nums="1"}else{if(av.curr==av.pTotal-2){nums=av.pTotal-2}else{nums=(av.curr)+"-"+(av.curr+1)}}if(av.covers){if(av.curr==0){nums=""}else{if(av.curr==av.pTotal-2){nums=""}else{nums=(av.curr-1)+"-"+(av.curr)}}}}b(av.menu+" .b-selector-page .b-current").text(nums)}}if(av.chapterSelector){if(K[av.curr]!=""){b(av.menu+" .b-selector-chapter .b-current").text(K[av.curr])}else{if(K[av.curr+1]!=""){b(av.menu+" .b-selector-chapter .b-current").text(K[av.curr+1])}}if(av.direction=="RTL"&&K[av.curr+1]!=""){b(av.menu+" .b-selector-chapter .b-current").text(K[av.curr+1])}else{if(K[av.curr]!=""){b(av.menu+" .b-selector-chapter .b-current").text(K[av.curr])}}}}function f(){O=x();if(!isNaN(O)&&O<=av.pTotal-1&&O>=0&&O!=""){if((O%2)!=0){O--}av.curr=O}else{M(av.curr+1,av)}ao.hash=O}function V(){O=x();if(!isNaN(O)&&O<=av.pTotal-1&&O>=0){if(O!=av.curr&&O.toString()!=ao.hash){if((O%2)!=0){O--}document.title=av.name+" - Page "+(O+1);if(!ad){ao.gotoPage(O);ao.hash=O}}}}function x(){var i=window.location.hash.split("/");if(i.length>1){return parseInt(i[2])-1}else{return""}}function M(j,i){if(i.hash){window.location.hash="/page/"+j}}}b.fn.booklet.interfaces=[];b.fn.booklet.defaults={name:null,width:600,height:400,speed:1000,direction:"LTR",startingPage:0,easing:"easeInOutQuad",easeIn:"easeInQuad",easeOut:"easeOutQuad",closed:false,closedFrontTitle:null,closedFrontChapter:null,closedBackTitle:null,closedBackChapter:null,covers:false,pagePadding:10,pageNumbers:true,hovers:true,overlays:true,tabs:false,tabWidth:60,tabHeight:20,arrows:false,cursor:"pointer",hash:false,keyboard:true,next:null,prev:null,menu:null,pageSelector:false,chapterSelector:false,shadows:true,shadowTopFwdWidth:166,shadowTopBackWidth:166,shadowBtmWidth:50,before:function(){},after:function(){}}})(jQuery);
 
-
/*
 
-
* jQuery Easing v1.3 - http://gsgd.co.uk/sandbox/jquery/easing/
 
-
*
 
-
* Uses the built in easing capabilities added In jQuery 1.1
 
-
* to offer multiple easing options
 
-
*
 
-
* TERMS OF USE - jQuery Easing
 
-
*
 
-
* Open source under the BSD License.
 
-
*
 
-
* Copyright © 2008 George McGinley Smith
 
-
* All rights reserved.
 
-
*
 
-
* Redistribution and use in source and binary forms, with or without modification,
 
-
* are permitted provided that the following conditions are met:
 
-
*
 
-
* Redistributions of source code must retain the above copyright notice, this list of
 
-
* conditions and the following disclaimer.
 
-
* Redistributions in binary form must reproduce the above copyright notice, this list
 
-
* of conditions and the following disclaimer in the documentation and/or other materials
 
-
* provided with the distribution.
 
-
*
 
-
* Neither the name of the author nor the names of contributors may be used to endorse
 
-
* or promote products derived from this software without specific prior written permission.
 
-
*
 
-
* THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY
 
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b.contentType);if(b.ifModified){c.lastModified[b.url]&&w.setRequestHeader("If-Modified-Since",c.lastModified[b.url]);c.etag[b.url]&&w.setRequestHeader("If-None-Match",c.etag[b.url])}o||w.setRequestHeader("X-Requested-With","XMLHttpRequest");w.setRequestHeader("Accept",b.dataType&&b.accepts[b.dataType]?b.accepts[b.dataType]+", */*; q=0.01":b.accepts._default)}catch(I){}if(b.beforeSend&&b.beforeSend.call(b.context,w,b)===false){b.global&&c.active--===1&&c.event.trigger("ajaxStop");w.abort();return false}b.global&&
 
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c.triggerGlobal(b,"ajaxSend",[w,b]);var L=w.onreadystatechange=function(m){if(!w||w.readyState===0||m==="abort"){J||c.handleComplete(b,w,e,f);J=true;if(w)w.onreadystatechange=c.noop}else if(!J&&w&&(w.readyState===4||m==="timeout")){J=true;w.onreadystatechange=c.noop;e=m==="timeout"?"timeout":!c.httpSuccess(w)?"error":b.ifModified&&c.httpNotModified(w,b.url)?"notmodified":"success";var p;if(e==="success")try{f=c.httpData(w,b.dataType,b)}catch(q){e="parsererror";p=q}if(e==="success"||e==="notmodified")d||
 
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encodeURIComponent(h)+"="+encodeURIComponent(l)};if(b===B)b=c.ajaxSettings.traditional;if(c.isArray(a)||a.jquery)c.each(a,function(){e(this.name,this.value)});else for(var f in a)da(f,a[f],b,e);return d.join("&").replace(tb,"+")}});c.extend({active:0,lastModified:{},etag:{},handleError:function(a,b,d,e){a.error&&a.error.call(a.context,b,d,e);a.global&&c.triggerGlobal(a,"ajaxError",[b,a,e])},handleSuccess:function(a,b,d,e){a.success&&a.success.call(a.context,e,d,b);a.global&&c.triggerGlobal(a,"ajaxSuccess",
 
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[b,a])},handleComplete:function(a,b,d){a.complete&&a.complete.call(a.context,b,d);a.global&&c.triggerGlobal(a,"ajaxComplete",[b,a]);a.global&&c.active--===1&&c.event.trigger("ajaxStop")},triggerGlobal:function(a,b,d){(a.context&&a.context.url==null?c(a.context):c.event).trigger(b,d)},httpSuccess:function(a){try{return!a.status&&location.protocol==="file:"||a.status>=200&&a.status<300||a.status===304||a.status===1223}catch(b){}return false},httpNotModified:function(a,b){var d=a.getResponseHeader("Last-Modified"),
 
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e=a.getResponseHeader("Etag");if(d)c.lastModified[b]=d;if(e)c.etag[b]=e;return a.status===304},httpData:function(a,b,d){var e=a.getResponseHeader("content-type")||"",f=b==="xml"||!b&&e.indexOf("xml")>=0;a=f?a.responseXML:a.responseText;f&&a.documentElement.nodeName==="parsererror"&&c.error("parsererror");if(d&&d.dataFilter)a=d.dataFilter(a,b);if(typeof a==="string")if(b==="json"||!b&&e.indexOf("json")>=0)a=c.parseJSON(a);else if(b==="script"||!b&&e.indexOf("javascript")>=0)c.globalEval(a);return a}});
 
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if(E.ActiveXObject)c.ajaxSettings.xhr=function(){if(E.location.protocol!=="file:")try{return new E.XMLHttpRequest}catch(a){}try{return new E.ActiveXObject("Microsoft.XMLHTTP")}catch(b){}};c.support.ajax=!!c.ajaxSettings.xhr();var ea={},vb=/^(?:toggle|show|hide)$/,wb=/^([+\-]=)?([\d+.\-]+)(.*)$/,ba,pa=[["height","marginTop","marginBottom","paddingTop","paddingBottom"],["width","marginLeft","marginRight","paddingLeft","paddingRight"],["opacity"]];c.fn.extend({show:function(a,b,d){if(a||a===0)return this.animate(S("show",
 
-
3),a,b,d);else{d=0;for(var e=this.length;d<e;d++){a=this[d];b=a.style.display;if(!c.data(a,"olddisplay")&&b==="none")b=a.style.display="";b===""&&c.css(a,"display")==="none"&&c.data(a,"olddisplay",qa(a.nodeName))}for(d=0;d<e;d++){a=this[d];b=a.style.display;if(b===""||b==="none")a.style.display=c.data(a,"olddisplay")||""}return this}},hide:function(a,b,d){if(a||a===0)return this.animate(S("hide",3),a,b,d);else{a=0;for(b=this.length;a<b;a++){d=c.css(this[a],"display");d!=="none"&&c.data(this[a],"olddisplay",
 
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d)}for(a=0;a<b;a++)this[a].style.display="none";return this}},_toggle:c.fn.toggle,toggle:function(a,b,d){var e=typeof a==="boolean";if(c.isFunction(a)&&c.isFunction(b))this._toggle.apply(this,arguments);else a==null||e?this.each(function(){var f=e?a:c(this).is(":hidden");c(this)[f?"show":"hide"]()}):this.animate(S("toggle",3),a,b,d);return this},fadeTo:function(a,b,d,e){return this.filter(":hidden").css("opacity",0).show().end().animate({opacity:b},a,d,e)},animate:function(a,b,d,e){var f=c.speed(b,
 
-
d,e);if(c.isEmptyObject(a))return this.each(f.complete);return this[f.queue===false?"each":"queue"](function(){var h=c.extend({},f),l,k=this.nodeType===1,o=k&&c(this).is(":hidden"),x=this;for(l in a){var r=c.camelCase(l);if(l!==r){a[r]=a[l];delete a[l];l=r}if(a[l]==="hide"&&o||a[l]==="show"&&!o)return h.complete.call(this);if(k&&(l==="height"||l==="width")){h.overflow=[this.style.overflow,this.style.overflowX,this.style.overflowY];if(c.css(this,"display")==="inline"&&c.css(this,"float")==="none")if(c.support.inlineBlockNeedsLayout)if(qa(this.nodeName)===
 
-
"inline")this.style.display="inline-block";else{this.style.display="inline";this.style.zoom=1}else this.style.display="inline-block"}if(c.isArray(a[l])){(h.specialEasing=h.specialEasing||{})[l]=a[l][1];a[l]=a[l][0]}}if(h.overflow!=null)this.style.overflow="hidden";h.curAnim=c.extend({},a);c.each(a,function(A,C){var J=new c.fx(x,h,A);if(vb.test(C))J[C==="toggle"?o?"show":"hide":C](a);else{var w=wb.exec(C),I=J.cur()||0;if(w){var L=parseFloat(w[2]),g=w[3]||"px";if(g!=="px"){c.style(x,A,(L||1)+g);I=(L||
 
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1)/J.cur()*I;c.style(x,A,I+g)}if(w[1])L=(w[1]==="-="?-1:1)*L+I;J.custom(I,L,g)}else J.custom(I,C,"")}});return true})},stop:function(a,b){var d=c.timers;a&&this.queue([]);this.each(function(){for(var e=d.length-1;e>=0;e--)if(d[e].elem===this){b&&d[e](true);d.splice(e,1)}});b||this.dequeue();return this}});c.each({slideDown:S("show",1),slideUp:S("hide",1),slideToggle:S("toggle",1),fadeIn:{opacity:"show"},fadeOut:{opacity:"hide"},fadeToggle:{opacity:"toggle"}},function(a,b){c.fn[a]=function(d,e,f){return this.animate(b,
 
-
d,e,f)}});c.extend({speed:function(a,b,d){var e=a&&typeof a==="object"?c.extend({},a):{complete:d||!d&&b||c.isFunction(a)&&a,duration:a,easing:d&&b||b&&!c.isFunction(b)&&b};e.duration=c.fx.off?0:typeof e.duration==="number"?e.duration:e.duration in c.fx.speeds?c.fx.speeds[e.duration]:c.fx.speeds._default;e.old=e.complete;e.complete=function(){e.queue!==false&&c(this).dequeue();c.isFunction(e.old)&&e.old.call(this)};return e},easing:{linear:function(a,b,d,e){return d+e*a},swing:function(a,b,d,e){return(-Math.cos(a*
 
-
Math.PI)/2+0.5)*e+d}},timers:[],fx:function(a,b,d){this.options=b;this.elem=a;this.prop=d;if(!b.orig)b.orig={}}});c.fx.prototype={update:function(){this.options.step&&this.options.step.call(this.elem,this.now,this);(c.fx.step[this.prop]||c.fx.step._default)(this)},cur:function(){if(this.elem[this.prop]!=null&&(!this.elem.style||this.elem.style[this.prop]==null))return this.elem[this.prop];var a=parseFloat(c.css(this.elem,this.prop));return a&&a>-1E4?a:0},custom:function(a,b,d){function e(l){return f.step(l)}
 
-
var f=this,h=c.fx;this.startTime=c.now();this.start=a;this.end=b;this.unit=d||this.unit||"px";this.now=this.start;this.pos=this.state=0;e.elem=this.elem;if(e()&&c.timers.push(e)&&!ba)ba=setInterval(h.tick,h.interval)},show:function(){this.options.orig[this.prop]=c.style(this.elem,this.prop);this.options.show=true;this.custom(this.prop==="width"||this.prop==="height"?1:0,this.cur());c(this.elem).show()},hide:function(){this.options.orig[this.prop]=c.style(this.elem,this.prop);this.options.hide=true;
 
-
this.custom(this.cur(),0)},step:function(a){var b=c.now(),d=true;if(a||b>=this.options.duration+this.startTime){this.now=this.end;this.pos=this.state=1;this.update();this.options.curAnim[this.prop]=true;for(var e in this.options.curAnim)if(this.options.curAnim[e]!==true)d=false;if(d){if(this.options.overflow!=null&&!c.support.shrinkWrapBlocks){var f=this.elem,h=this.options;c.each(["","X","Y"],function(k,o){f.style["overflow"+o]=h.overflow[k]})}this.options.hide&&c(this.elem).hide();if(this.options.hide||
 
-
this.options.show)for(var l in this.options.curAnim)c.style(this.elem,l,this.options.orig[l]);this.options.complete.call(this.elem)}return false}else{a=b-this.startTime;this.state=a/this.options.duration;b=this.options.easing||(c.easing.swing?"swing":"linear");this.pos=c.easing[this.options.specialEasing&&this.options.specialEasing[this.prop]||b](this.state,a,0,1,this.options.duration);this.now=this.start+(this.end-this.start)*this.pos;this.update()}return true}};c.extend(c.fx,{tick:function(){for(var a=
 
-
c.timers,b=0;b<a.length;b++)a[b]()||a.splice(b--,1);a.length||c.fx.stop()},interval:13,stop:function(){clearInterval(ba);ba=null},speeds:{slow:600,fast:200,_default:400},step:{opacity:function(a){c.style(a.elem,"opacity",a.now)},_default:function(a){if(a.elem.style&&a.elem.style[a.prop]!=null)a.elem.style[a.prop]=(a.prop==="width"||a.prop==="height"?Math.max(0,a.now):a.now)+a.unit;else a.elem[a.prop]=a.now}}});if(c.expr&&c.expr.filters)c.expr.filters.animated=function(a){return c.grep(c.timers,function(b){return a===
 
-
b.elem}).length};var xb=/^t(?:able|d|h)$/i,Ia=/^(?:body|html)$/i;c.fn.offset="getBoundingClientRect"in t.documentElement?function(a){var b=this[0],d;if(a)return this.each(function(l){c.offset.setOffset(this,a,l)});if(!b||!b.ownerDocument)return null;if(b===b.ownerDocument.body)return c.offset.bodyOffset(b);try{d=b.getBoundingClientRect()}catch(e){}var f=b.ownerDocument,h=f.documentElement;if(!d||!c.contains(h,b))return d||{top:0,left:0};b=f.body;f=fa(f);return{top:d.top+(f.pageYOffset||c.support.boxModel&&
 
-
h.scrollTop||b.scrollTop)-(h.clientTop||b.clientTop||0),left:d.left+(f.pageXOffset||c.support.boxModel&&h.scrollLeft||b.scrollLeft)-(h.clientLeft||b.clientLeft||0)}}:function(a){var b=this[0];if(a)return this.each(function(x){c.offset.setOffset(this,a,x)});if(!b||!b.ownerDocument)return null;if(b===b.ownerDocument.body)return c.offset.bodyOffset(b);c.offset.initialize();var d,e=b.offsetParent,f=b.ownerDocument,h=f.documentElement,l=f.body;d=(f=f.defaultView)?f.getComputedStyle(b,null):b.currentStyle;
 
-
for(var k=b.offsetTop,o=b.offsetLeft;(b=b.parentNode)&&b!==l&&b!==h;){if(c.offset.supportsFixedPosition&&d.position==="fixed")break;d=f?f.getComputedStyle(b,null):b.currentStyle;k-=b.scrollTop;o-=b.scrollLeft;if(b===e){k+=b.offsetTop;o+=b.offsetLeft;if(c.offset.doesNotAddBorder&&!(c.offset.doesAddBorderForTableAndCells&&xb.test(b.nodeName))){k+=parseFloat(d.borderTopWidth)||0;o+=parseFloat(d.borderLeftWidth)||0}e=b.offsetParent}if(c.offset.subtractsBorderForOverflowNotVisible&&d.overflow!=="visible"){k+=
 
-
parseFloat(d.borderTopWidth)||0;o+=parseFloat(d.borderLeftWidth)||0}d=d}if(d.position==="relative"||d.position==="static"){k+=l.offsetTop;o+=l.offsetLeft}if(c.offset.supportsFixedPosition&&d.position==="fixed"){k+=Math.max(h.scrollTop,l.scrollTop);o+=Math.max(h.scrollLeft,l.scrollLeft)}return{top:k,left:o}};c.offset={initialize:function(){var a=t.body,b=t.createElement("div"),d,e,f,h=parseFloat(c.css(a,"marginTop"))||0;c.extend(b.style,{position:"absolute",top:0,left:0,margin:0,border:0,width:"1px",
 
-
height:"1px",visibility:"hidden"});b.innerHTML="<div style='position:absolute;top:0;left:0;margin:0;border:5px solid #000;padding:0;width:1px;height:1px;'><div></div></div><table style='position:absolute;top:0;left:0;margin:0;border:5px solid #000;padding:0;width:1px;height:1px;' cellpadding='0' cellspacing='0'><tr><td></td></tr></table>";a.insertBefore(b,a.firstChild);d=b.firstChild;e=d.firstChild;f=d.nextSibling.firstChild.firstChild;this.doesNotAddBorder=e.offsetTop!==5;this.doesAddBorderForTableAndCells=
 
-
f.offsetTop===5;e.style.position="fixed";e.style.top="20px";this.supportsFixedPosition=e.offsetTop===20||e.offsetTop===15;e.style.position=e.style.top="";d.style.overflow="hidden";d.style.position="relative";this.subtractsBorderForOverflowNotVisible=e.offsetTop===-5;this.doesNotIncludeMarginInBodyOffset=a.offsetTop!==h;a.removeChild(b);c.offset.initialize=c.noop},bodyOffset:function(a){var b=a.offsetTop,d=a.offsetLeft;c.offset.initialize();if(c.offset.doesNotIncludeMarginInBodyOffset){b+=parseFloat(c.css(a,
 
-
"marginTop"))||0;d+=parseFloat(c.css(a,"marginLeft"))||0}return{top:b,left:d}},setOffset:function(a,b,d){var e=c.css(a,"position");if(e==="static")a.style.position="relative";var f=c(a),h=f.offset(),l=c.css(a,"top"),k=c.css(a,"left"),o=e==="absolute"&&c.inArray("auto",[l,k])>-1;e={};var x={};if(o)x=f.position();l=o?x.top:parseInt(l,10)||0;k=o?x.left:parseInt(k,10)||0;if(c.isFunction(b))b=b.call(a,d,h);if(b.top!=null)e.top=b.top-h.top+l;if(b.left!=null)e.left=b.left-h.left+k;"using"in b?b.using.call(a,
 
-
e):f.css(e)}};c.fn.extend({position:function(){if(!this[0])return null;var a=this[0],b=this.offsetParent(),d=this.offset(),e=Ia.test(b[0].nodeName)?{top:0,left:0}:b.offset();d.top-=parseFloat(c.css(a,"marginTop"))||0;d.left-=parseFloat(c.css(a,"marginLeft"))||0;e.top+=parseFloat(c.css(b[0],"borderTopWidth"))||0;e.left+=parseFloat(c.css(b[0],"borderLeftWidth"))||0;return{top:d.top-e.top,left:d.left-e.left}},offsetParent:function(){return this.map(function(){for(var a=this.offsetParent||t.body;a&&!Ia.test(a.nodeName)&&
 
-
c.css(a,"position")==="static";)a=a.offsetParent;return a})}});c.each(["Left","Top"],function(a,b){var d="scroll"+b;c.fn[d]=function(e){var f=this[0],h;if(!f)return null;if(e!==B)return this.each(function(){if(h=fa(this))h.scrollTo(!a?e:c(h).scrollLeft(),a?e:c(h).scrollTop());else this[d]=e});else return(h=fa(f))?"pageXOffset"in h?h[a?"pageYOffset":"pageXOffset"]:c.support.boxModel&&h.document.documentElement[d]||h.document.body[d]:f[d]}});c.each(["Height","Width"],function(a,b){var d=b.toLowerCase();
 
-
c.fn["inner"+b]=function(){return this[0]?parseFloat(c.css(this[0],d,"padding")):null};c.fn["outer"+b]=function(e){return this[0]?parseFloat(c.css(this[0],d,e?"margin":"border")):null};c.fn[d]=function(e){var f=this[0];if(!f)return e==null?null:this;if(c.isFunction(e))return this.each(function(l){var k=c(this);k[d](e.call(this,l,k[d]()))});if(c.isWindow(f))return f.document.compatMode==="CSS1Compat"&&f.document.documentElement["client"+b]||f.document.body["client"+b];else if(f.nodeType===9)return Math.max(f.documentElement["client"+
 
-
b],f.body["scroll"+b],f.documentElement["scroll"+b],f.body["offset"+b],f.documentElement["offset"+b]);else if(e===B){f=c.css(f,d);var h=parseFloat(f);return c.isNaN(h)?f:h}else return this.css(d,typeof e==="string"?e:e+"px")}})})(window);
 
-
/* @Booklet
 
-
----------------------------------------*/
 
-
.booklet          {width:800px; height:600px; position:relative; /*background:#fff;*/ margin:0 auto 10px;}
 
-
.booklet .b-page  {left:0; top:0; position:absolute; overflow:hidden; padding:0;}
 
-
 
-
.booklet .b-pN  {}
 
-
.booklet .b-p0  {}
 
-
.booklet .b-p1  {}
 
-
.booklet .b-p2  {}   
 
-
.booklet .b-p3  {}
 
-
.booklet .b-p4  {}
 
-
 
-
/*.booklet .b-load  {display:none;}*/
 
-
 
 
-
  /* Page Wrappers */
 
-
.booklet .b-wrap      {top:0; position:absolute;}
 
-
.booklet .b-wrap-left  {background:#fff;}
 
-
.booklet .b-wrap-right {background:#efefef;}
 
-
 
-
.booklet .b-pN .b-wrap,
 
-
.booklet .b-p1 .b-wrap,
 
-
.booklet .b-p2 .b-wrap,
 
-
.booklet .b-p3 .b-wrap,
 
-
.booklet .b-p4 .b-wrap  {left:0;}
 
-
.booklet .b-p0 .b-wrap  {right:0;}
 
-
 
-
  /* Custom Page Types */
 
-
.booklet .b-page-blank  {padding:0; width:100%; height:100%;}
 
-
.booklet .b-page-cover  {padding:0; width:100%; height:100%; background:#925C0E;}
 
-
.booklet .b-page-cover h3  {color:#fff; text-shadow:0px 1px 3px #222;}
 
-
 
 
-
  /* Page Numbers */
 
-
.booklet .b-counter {bottom:10px; position:absolute; display:block; width:25px; height:20px; background:#ccc; color:#444; text-align:center; font-family:Georgia, "Times New Roman", Times, serif; font-size:10px; padding:5px 0 0;}
 
-
.booklet .b-wrap-left  .b-counter  {left:10px;}
 
-
.booklet .b-wrap-right .b-counter {right:10px;}
 
-
 
 
-
  /* Page Shadows */
 
-
.booklet .b-shadow-f  {right:0; top:0; position:absolute; opacity:0; background-image:url("images/shadow-top-forward.png"); background-repeat:repeat-y; background-position:100% 0;}
 
-
.booklet .b-shadow-b  {left:0;  top:0; position:absolute; opacity:0; background-image:url("images/shadow-top-back.png");    background-repeat:repeat-y; background-position:0 0;}
 
-
 
-
.booklet .b-p0 {background-image:url("images/shadow.png"); background-repeat:repeat-y; background-position:100% 10px;}
 
-
.booklet .b-p3 {background-image:url("images/shadow.png"); background-repeat:repeat-y; background-position:0 10px;}
 
-
 
-
  /* Overlay Controls */
 
-
.booklet .b-overlay      {top:0; position:absolute; height:100%; width:50%;}
 
-
.booklet .b-overlay-prev {left:0;}
 
-
.booklet .b-overlay-next {right:0;}
 
-
 
-
  /* Tab Controls */
 
-
.booklet .b-tab        {background:#000; height:40px; width:100%; padding:10px 0 0; text-align:center; color:#ccc; position:absolute; font-size:11px;}
 
-
.booklet .b-tab-prev  {left:0;}
 
-
.booklet .b-tab-next  {right:0;}
 
-
.booklet .b-tab:hover  {background:#aaa; color:#000;}
 
-
 
-
  /* Arrow Controls */
 
-
.booklet .b-arrow          {display:block; position:absolute; text-indent:-9999px; top:0; height:100%; width:74px;}
 
-
.booklet .b-arrow div      {display:none; text-indent:-9999px; height:170px; width:74px; position:absolute; left:0; top:25%;}
 
-
.booklet .b-arrow-next    {right:-80px;}
 
-
.booklet .b-arrow-prev    {left:-80px;}
 
-
.booklet .b-arrow-next div {background:url("images/arrow-next.png") no-repeat left top;}
 
-
.booklet .b-arrow-prev div {background:url("images/arrow-prev.png") no-repeat left top;}
 
-
 
-
/* @z-index fix (needed for older IE browsers)
 
-
----------------------------------------*/
 
-
body              {z-index:0;}
 
-
    .b-menu          {z-index:100;}
 
-
    .b-selector      {z-index:100;}
 
-
.booklet          {z-index:10;}
 
-
    .b-pN            {z-index:10;}
 
-
    .b-p0            {z-index:10;}
 
-
    .b-p1            {z-index:10;}
 
-
    .b-p2            {z-index:10;}
 
-
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<body>
 
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<h1 class="title">              Protocols //Notebook </h1>
 
-
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          <div> <img alt="" src="https://static.igem.org/mediawiki/2014hs/8/88/FotoiGEM1.jpg" />
 
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            <h1>Protocol 1 </h1>
 
-
            <p>Preparation of culture media (1L).<br />
 
-
              1.- 5 g of yeast<br />
 
-
              2.- 10 g of sodium chloride.<br />
 
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              3.- 10 g of triptone.<br />
 
-
              4.- Add water till 1 uL volume.</p>
 
-
          </div>
 
-
          <div>
 
-
            <h1>Protocol 2</h1>
 
-
            <p>Competent cell´s preparation<br />
 
-
              <br />
 
-
              1.- Centrifug your media culture tube (25 mL) at 4,000 rpm during
 
-
              15 min.<br />
 
-
              2.- Pour the supernatant and add 10 mL of cold MgCl2 solution.
 
-
              Centrifuge them at 4,000 rpm at 4 °C during 15 min.<br />
 
-
              3.- Pour the supernatant and resuspend the pellet in 40 mL of cold
 
-
              CaCl2 solution. Keep it in ice during 20 min and place them in
 
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              eppendorf tubes. Centrifuge them at 4,000 rpm at 4°C during 15
 
-
              min.<br />
 
-
              4.- Pour the supernatant and resuspend the pellet in 40 mL of cold
 
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              85mM CaCl2/15% glycerlol v/v solution. Centrifuge again at 2,100
 
-
              rpm at 4°C during 15 min.<br />
 
-
              5.- Pour the supernatant and resuspend in 2 mL of cold 85bmM
 
-
              CaCl2/15% glycerol v/v solution.<br />
 
-
              6.- Aliquote in tubes of 100 uL previously frozen and store at
 
-
              -80°C.<br />
 
-
            </p>
 
-
          </div>
 
-
          <div>
 
-
            <h1>Protocol 3</h1>
 
-
            <p>DNA extraction from Buffy coat:<br />
 
-
              <br />
 
-
              <font size="1"> 1.- Centrifuge 5 mL of culture medium at 1,500 rpm
 
-
                for 10 min at 4°C.</font><br />
 
-
              <font size="1">2.- Carefully transfer 600 uL of buffy coat to a
 
-
                microtube.</font><br />
 
-
              <font size="1">3.- Add 1 mL of RBC buffer and mix throughly by
 
-
                vortex.</font><br />
 
-
              <font size="1">4.- Centrifuge at 4,500 rpm during 30 s and pour
 
-
                carefully so your pellet is not wasted.</font><br />
 
-
              <font size="1">5.- Repeat steps 2 and 3 adding just 500 uL from
 
-
                RBC buffer.</font><br />
 
-
              <font size="1">6.- Once separated, add 300 uL of lysis buffer to
 
-
                the pellet and mix by vortex.</font><br />
 
-
              <font size="1">7.- Add 150 uL of protein precipitation solution.</font><br />
 
-
              <font size="1">8.- Centrifuge at 11,000 rpm during 5 min; then,
 
-
                transfer the supernatant to a new microtube.</font><br />
 
-
              <font size="1">9.- Add 700 uL of cold isopropanol and mix by
 
-
                inverting until you see a white formation within the tube.</font><br />
 
-
              <font size="1">10.- Centrifuge at 11,000 rpm during 4 min and pour
 
-
                carefully taking care of the pellet at the bottom.</font><br />
 
-
              <font size="1">11.- Add 500 uL of cold ethanol 70% to wash and
 
-
                then centrifuge at 11,000 rpm during 4 min.</font><br />
 
-
              <font size="1">12.- Pour and let the ethanol evaporate at room
 
-
                temperature.</font><br />
 
-
              <font size="1"> 13.- Resuspend the DNA pellet with 12 uL of TE
 
-
                buffer; incubate all night at 4 °C.</font><br />
 
-
            </p>
 
-
          </div>
 
-
          <div>
 
-
            <h1>Protocol 4</h1>
 
-
            <p>Electrophoresis:<br />
 
-
              <br />
 
-
              <font size="1">1.- Prepare 1L of TAE 10X (48.4g of Tris base[ tris
 
-
                (hidroxymethyl) amminomethane], 11.4 mL of glacial acetic acid
 
-
                (17.4 M) and 3.7 g of EDTA then fill with deionized water to 1
 
-
                L.</font><br />
 
-
              <font size="1">2.- Prepare a 50 mL solution of 3% agarose using
 
-
                TAE buffer (without using water).</font><br />
 
-
              <font size="1">3.- Heat the gel in the microwave in intervals of
 
-
                30 s, 20 s and 10 s until the agarose is completely dissolved,
 
-
                taking care that it does not boil so much.</font><br />
 
-
              <font size="1">4.- Let cool down the gel (55-60°C) and pour in a
 
-
                base with the comb already on its place to form the charging
 
-
                wells.</font><br />
 
-
              <font size="1">5.- Wait until the gel solidifies.</font><br />
 
-
              <font size="1">6.- Remove the comb and place the base with the gel
 
-
                inside the electrophoretic chamber.</font><br />
 
-
              <font size="1">7.- Add TAE buffer until the gel is covered.</font><br />
 
-
              <font size="1">8.- Take 5 uL of each sample from the PCR and 1 uL
 
-
                of charging gel in a new PCR tube, mix well.</font><br />
 
-
              <font size="1">9.- Charge the samples inside the wells of the gel,
 
-
                adding a weight marcker.</font><br />
 
-
              <font size="1">10.- Close the electrophoretic chamber and run at
 
-
                120 V during 30 min. Follow the displacement ofthe samples.</font><br />
 
-
              <font size="1">11.- Take out the gel from the chamber very
 
-
                carefully and place in the UV light to see the DNA strands.</font><br />
 
-
            </p>
 
-
          </div>
 
-
          <div>
 
-
            <h1>Protocol 5</h1>
 
-
            <p>Competent cell transformation:<br />
 
-
              <br />
 
-
              1.- Place 1 uL of the plasmid in a microtube, gently add 100 uL of
 
-
              your competent cells. Take another tube and place only 100 uL of
 
-
              competent cells, so you have a control group.<br />
 
-
              2.- Mix gently with a pipette and incubate during 30 min in ice.<br />
 
-
              3.- Ab ruptly place your cells in 42°C dry bath during 2 min and
 
-
              replace in ice whwn finished.<br />
 
-
              4.- Add 900 uL of LB medium and incubate at 37 °C for 30 min.<br />
 
-
              5.- Inoculate with 200 uL of your transformed cells in LB-Amp.<br />
 
-
              6.- Incubate overnight and store at 4 °C of freeze in 15-25%
 
-
              glycerol.<br />
 
-
            </p>
 
-
          </div>
 
-
          <div> <img alt="" src="https://static.igem.org/mediawiki/2014hs/e/e4/LabArchives.jpg" />
 
-
            <h1>Lab archives// Mar 12, 2014 </h1>
 
-
            <p>In order to grow our bacteria, we inoculated with 100 uL of E.
 
-
              Coli DH5-alpha in 50 mL of LB medium, and 100 uL of E. Coli TOP in
 
-
              50 mL of LB medium. We left both samples incubating overnight </p>
 
-
            <h1>Lab archives// Mar 20, 2014 </h1>
 
-
            <p>We inoculated E.Coli NEB10-beta in 50 ml of LB medium. </p>
 
-
          </div>
 
-
          <div>
 
-
            <h1>Lab archives// Mar 27, 2014 </h1>
 
-
            <p>We prepared the medium in which our bacteria will grow, for that
 
-
              we dissolved solid LB with agarose, then we add it into 10 plates,
 
-
              we waited a few minutes for it to solidify and finally we added
 
-
              500 uL of our antibiotic that is chloranphenicol to each of those
 
-
              plates. </p>
 
-
            <h1>Lab archives// Apr 01, 2014 </h1>
 
-
            <p>we transformed luciferaze from DNA to cells by incerting them
 
-
              with change of temperature </p>
 
-
            <h1>Lab archives// Apr 02, 2014 </h1>
 
-
            <p>We made a litter of agar and 500 L of growth medium, and we
 
-
              sterilized them along with crystal media disperssion balls </p>
 
-
            <h1>Lab archives// Apr 03, 2014 </h1>
 
-
            <p>Today we prepared buffers for home made mini preps and did a cell
 
-
              re culture. </p>
 
-
          </div>
 
-
          <div>
 
-
            <h1><font size="1">Lab archives// Apr 07, 2014 </font> </h1>
 
-
            <p> <font size="1">We added 700 mL of water in a beaker and then we
 
-
                prepared 23 grams of powdered agar that we added slowly while we
 
-
                moved the beaker. This in order to help the dissolve the powder.
 
-
                After that, we added other 300 mL to the solution and finally we
 
-
                passed it to an Erlenmeyer matrass. Also we prepared 500 mL of
 
-
                growth medium. First, we measured and added 5 grams of tryptone,
 
-
                5 grams of yeast and 10 grams of sodium chloride. Those
 
-
                ingredients were added slowly to a graduated cylinder with 350
 
-
                mL of wáter while the flask was moved. After finishing the
 
-
                addition we added other 150 mL of water. The solution was then
 
-
                transfered to another Erlenmeyer flask. Both Erlenmeyer flasks
 
-
                and a glass bottle with crystal media diperssion balls were
 
-
                sterilized with temperature and pressure.</font> </p>
 
-
            <h1><font size="1">Lab archives// Apr 02, 2014 </font> </h1>
 
-
            <p><font size="1">300 ul of ampicillin at 100mg/ml chloramphenicol </font><br />
 
-
              <font size="1"> 3 Plates with agar </font><br />
 
-
              <font size="1">Promoters: BBa_K258005 (pO2), BBa_176500 (pFe)
 
-
                BBa_J52008 (luc-3) </font> <br />
 
-
              <font size="1">The plates were impregnated with ampicillin
 
-
                promoting bacteria in the following manner </font><br />
 
-
              <font size="1"> |pO2 | Plate | 150 ul Ampiciline | K258005 | </font><br />
 
-
              <font size="1">
 
-
                ------------------------------------------------------ </font><br />
 
-
              <font size="1"> |pFe | Plate | 150 ul Ampiciline | 1765000 | </font><br />
 
-
              <font size="1">
 
-
                ------------------------------------------------------ </font><br />
 
-
              <font size="1"> |Luc-3 | Plate | Chloranphenicol | J52008 | </font><br />
 
-
            </p>
 
-
            <p></p>
 
-
          </div>
 
-
          <div>
 
-
            <h1><font size="1">Apr 08, 2014 </font></h1>
 
-
            <p> <font size="1"> **/Today we realized the mini prep for the
 
-
                oxygen promoter. /** </font><br />
 
-
              <font size="1"> --500 ul of Ampiciline //this is for the oxygen
 
-
                promoter </font><br />
 
-
              <font size="1"> --50 ul of oxygen promoter </font><br />
 
-
              <font size="1"> --45 ml of LB medium </font><br />
 
-
              <font size="1"> --at room temperature 15 minutes at 1500 rpm in
 
-
                the centrifuge. </font><br />
 
-
              <font size="1"> --500 ul of chloramphenicol // this is for the red
 
-
                liciferase </font><br />
 
-
              <font size="1"> --50 ul of red luciferase </font><br />
 
-
              <font size="1"> --45 ml of LB medium </font><br />
 
-
              <font size="1"> --The new LB medium was prepared with //LB medium
 
-
              </font><br />
 
-
              <font size="1"> --10 g yeast </font><br />
 
-
              <font size="1"> --5 g salt </font><br />
 
-
              <font size="1"> --15 g Tryptone </font> <font size="1"> MINI
 
-
                PREP First 400 ul of Genomic Lysis Buffer to the 40 ml overnight
 
-
                [bacteriaand oxygen promoter] Then the mixture is transferred to
 
-
                a Zyne-Spine Collumn in a Collection Tube. Centrifuge 10,000 rpm
 
-
                /1 min Transfer the Zyno Spin Column to a new Collection Tube.
 
-
                Add 200 micro liters of Pre Wash Buffer. Centrifuge 10,000 rpm
 
-
                /1 min Add 500 uL of of g-DNA Wash buffer to the spin column.
 
-
                Centrifuge 10,000 rpm /1 min Transfer the spin column to a clear
 
-
                micro centrifuge tube. Add 50 uL of DNA Elution Buffer .
 
-
                Incubate 2-5 minutes to at room temperature. Centrifuge at top
 
-
                speed per 30 seconds. Then store at -80 °C </font></p>
 
-
          </div>
 
-
          <div>
 
-
            <h1><font size="1"> Apr 22, 2014</font> </h1>
 
-
            <p><font size="1"> We prepared 2 overnight cultures (45ml of LB
 
-
                medium): GFP E0040 pSB1A2 with 100 uL of ampicillin. M-cherry
 
-
                (RFP) BBa_J04450 pSB1C3 with 500 uL of chloramphenicol.</font></p>
 
-
            <h1><font size="1"> Apr 24, 2014 </font></h1>
 
-
            <p><font size="1"> 2 Transformations: Bacteria NEB 10 Beta, DH5alpha
 
-
                2 Mini Preps: GFP, RFP //Transformation and preps:--
 
-
                Centrifugation at 2500 rpm for 15 min.--1ul DNA in 100 uL of
 
-
                bacterial culture (luciferase). --Filtering GFP, RFP bacteria.
 
-
                --Added 400 ml of Lysis Buffer Genomyc each crop by columns.
 
-
                --Centrifugation at 10,000 * g for 1 min. --Last column to
 
-
                another tube and DNA Prewash + 200 uL Buffer. --Centrifuged at
 
-
                10,000 * g for 1 min. --Add 500ul of DNA Wash Buffer,
 
-
                centrifuged at 10,000 * g for 1 min. --Transfer to clean tube,
 
-
                add 50ul of DNA Elution Buffer. --Incubate for 2-5 min at room
 
-
                temperature, centrifuged at maximum speed for 30 sec.</font></p>
 
-
            <h1><font size="1"> Apr 28, 2014 </font></h1>
 
-
            <p><font size="1"> Today was prepared LB media. It also was done the
 
-
                sterilization of 1 mL and 200 uL tips, water and ependorfs.
 
-
                After the sterilization of the LB media was done, we cultivated
 
-
                in it the bacteria carrying the oxygen promoter, RFP, and GFP.
 
-
                Then we left the bacteria growing in the incubator at 37°C
 
-
                overnight.</font></p>
 
-
          </div>
 
-
          <div>
 
-
            <h1><font size="1"> May 05, 2014 </font></h1>
 
-
            <p><font size="1"> Plating bacteria with GFP, RFP, promoter Oxygen
 
-
                and iron promoter plasmids. Everyone in their respective
 
-
                environments and specific antibiotics (ampicillin and
 
-
                chloramphenicol). Tubes with bacteria was added and placed them
 
-
                in LB medium incubator to grow more product if necessary.</font></p>
 
-
            <h1><font size="1"> May 08, 2014 </font></h1>
 
-
            <p><font size="1"> We did a centrifuge at 4800 rpm for 20 min for
 
-
                the plasmids had been in overnight which had oxygen promoter,
 
-
                GFP and RFP to rush after we separate the aqueous phase.</font></p>
 
-
            <h1><font size="1"> May 19, 2014 </font></h1>
 
-
            <p><font size="1"> We did inoculation of GFP, RFP and Oxigen Prmoter</font></p>
 
-
            <h1><font size="1"> May 20, 2014 </font></h1>
 
-
            <p><font size="1"> We started minipreps preparation of GFP, RFP and
 
-
                Oxigen Promoter. We added 1.5 ml of culture to each Eppendorff
 
-
                (4 for each promoter) and we centrifugated it at 13.2 rpm for 10
 
-
                minutes, we did the same procedure 2 times. Also, we sterilized
 
-
                two Erlenmeyer flasks and 40 Eppendorffs. </font></p>
 
-
          </div>
 
-
          <div>
 
-
            <h1><font size="1"> May 20, 2014 //Mini prep preparation </font></h1>
 
-
            <p><font size="1"> 1. We added 1.5 mL of the cultures to their
 
-
                corresponding Eppendorffs and centrifugated them at 3400 rpm
 
-
                during 10 minutes other three times.</font></p>
 
-
            <p><font size="1">2. Once we had a pellet we discarded the
 
-
                supernatant and we added 300 uL of TEG buffer (made of 100 mM of
 
-
                Tris-HCl pH8, 2 mM EDTA and 20% glucose)</font></p>
 
-
            <p><font size="1">3. We added 700 μL of NS solution (0.2 M NaOH, 1%
 
-
                w/v SDs) and we mixed by inversion.</font></p>
 
-
            <p><font size="1">4. We added 400 μL of 3 M sodium acetate pH 5.3,
 
-
                and we mixed by inversion.</font></p>
 
-
            <p><font size="1">5. We centrifugated the Eppendorffs at 3400 rpm
 
-
                during 15 minutes.</font></p>
 
-
            <p><font size="1">6. We transfered the supernatant to other
 
-
                Eppendorffs and we added 50 uL of cold isopropanol and mixed by
 
-
                inversion.</font></p>
 
-
            <p><font size="1">7. We centrifugated the Eppendorffs at 3400 rpm
 
-
                during 15 minutes.</font></p>
 
-
            <p><font size="1">8. We discarded the supernatant and washed each
 
-
                pellet with 70% ethanol and we let it dry for 15 minutes.</font></p>
 
-
            <p><font size="1">9. We resuspended the pellet in 500 uL of TE
 
-
                buffer and then we added 10 mg/mL RNAseA.</font></p>
 
-
            <p><font size="1">11. We precipitated the DNA with 100 uL of ethanol
 
-
                in each Eppendorff.</font></p>
 
-
            <p><font size="1">12. We centrifugated for 1 minute at 3400 rpm.</font></p>
 
-
            <p><font size="1">13. We got rid of the supernatant.</font></p>
 
-
            <p><font size="1">14. Finally, we resuspended the pellet in 50 uL of
 
-
                TE buffer</font></p>
 
-
          </div>
 
-
          <div> <img alt="" src="https://static.igem.org/mediawiki/2014hs/f/f4/1%281%29.jpg" />
 
-
            <h1>UNAM procedures// Digestion</h1>
 
-
            <p>|BSA | 1 uL | </p>
 
-
            <p>|DNA | 2 uL | </p>
 
-
            <p>|Buffer (2) | 1 uL |</p>
 
-
            <p>|Buffer Xba I+Pstl | 3.3 uL |</p>
 
-
            <p>|fe Xba I+Spef | 3.3 uL | </p>
 
-
            <p>|H<sub>2</sub>0 | 5.4 uL | </p>
 
-
          </div>
 
-
          <div>
 
-
            <h1><font size="1">Gel agarose</font></h1>
 
-
            <p><font size="1"> 1 kb NEB Ampyciline -&gt; Pentneryl </font></p>
 
-
            <p><font size="1">| ---------------Bristol - Myers Squibb</font> </p>
 
-
            <p><font size="1">| ---------------1 gram/ 3 mL</font> </p>
 
-
            <p><font size="1">| ---------------inyectable</font></p>
 
-
            <p><font size="1">|<sub>p</sub>O <sub>2</sub> | 3.3 uL</font> </p>
 
-
            <p><font size="1">|1 kb marker 5.4 uL </font></p>
 
-
            <h1><font size="1">Homemade Mini prep Preparation </font></h1>
 
-
            <p><font size="1"> 1.- Overnight bacterial culture of 5 mL in
 
-
                falkons of 50 mL </font></p>
 
-
            <p><font size="1"> 2.- Centrifugate in an eppendor of 1.5 mL and
 
-
                throw supernadant</font></p>
 
-
            <p><font size="1"> 3.- Add 152 uL of Buffer 1 + RNAse (11
 
-
                micro-liters /1 buffer micro-liter ) **Buffer 20% Glucouse </font></p>
 
-
            <p><font size="1"> 4.- Mix via Buffer and add 150 uL of Buffer 2
 
-
                *Wait 4-5 minutes*</font> </p>
 
-
            <p><font size="1"> (1.5mL)Buffer 2 -&gt; 880 uL H<sub>2</sub>O,20uL
 
-
                NaOH 10 N </font></p>
 
-
            <p><font size="1"> ---------------------------------------100 uL SDS
 
-
                10%</font> </p>
 
-
            <p><font size="1"> 5.- Mix via inversion and add150 uL Buffer 3 </font>
 
-
            </p>
 
-
            <p><font size="1"> ------------------------------Sodium Acetate 3M
 
-
                pH 5</font> </p>
 
-
          </div>
 
-
          <div>
 
-
            <p><font size="1"> 6.-Centrifuge 5 minutes.</font> </p>
 
-
            <p><font size="1"> 7.-Take the supernadant to antoher tube. Add 1 mL
 
-
                ETOH Absolute </font></p>
 
-
            <p><font size="1"> 8.-Centrifuge by 5 minutes and decant </font> </p>
 
-
            <p><font size="1"> 9.-Wash with 500uL ETOH 70 %</font> </p>
 
-
            <p><font size="1"> 10.-Centrifugate 1 minute </font></p>
 
-
            <p><font size="1"> 11.-Decant and dry at 37 celsius centigrades (30
 
-
                minutes)</font> </p>
 
-
            <p><font size="1"> 12.-Resuspend in 15 uL of H<sub>2</sub>O </font></p>
 
-
            <h1><font size="1">Ligation</font></h1>
 
-
            <p><font size="1"> Inserto 7mL</font></p>
 
-
            <p><font size="1"> Plasmid 3mL</font> </p>
 
-
            <p><font size="1"> Buffer 10x 1.5mL </font> </p>
 
-
            <p><font size="1"> Ligase 0.5 mL </font></p>
 
-
            <p><font size="1"> H<sub>2</sub>O 3 mL </font> </p>
 
-
            <p><font size="1"> Total = 15 ml </font></p>
 
-
          </div>
 
-
          <div>
 
-
            <h1>E.Coli cells transformation. </h1>
 
-
            <p>To transform E.Coli cells with an exogen DNA, 200 uL of competent
 
-
              cells were taken they were mixed with 1-20 ug(Micrograms) of DNA
 
-
              that was incubated on ice for 30 minutes. This makes posible the
 
-
              absorbtion of the exogen DNA to the cells surface, after that, the
 
-
              cells are incubated at 42Celsius Degrees during 90 seconds, for
 
-
              then incubate them during 1 minute again in ice. THis termal shock
 
-
              makes posible the entrance of the plasmid to the inside of the
 
-
              cell. 600 uL of LB medium were added and then incubated during
 
-
              60-90 minutes at 37 Celisus Degres, to do posible the segregation
 
-
              of the exogenic plasmid during the celular division (3 to 5
 
-
              generations). Finally, Alicuote of 100-200 uL were cultivated by
 
-
              dimention in the LB agar medium, which ere incubated at 37 Celsius
 
-
              Degrees. After 15-20 hours of incuvation colonies could be
 
-
              observed</p>
 
-
          </div>
 
-
          <div>
 
-
            <h1>Ligations</h1>
 
-
            <p>1# Grow h mL in overnight</p>
 
-
            <p>2# Do minipreps</p>
 
-
            <p>3# EcoRI and PstI restrictions.</p>
 
-
            <p> --for Fe EcoRIand SpeI</p>
 
-
            <p>O<sub>2</sub>+ GFP ---1,2,3 - 931</p>
 
-
            <p>Fe+GTP ---4,5,6 -1,828</p>
 
-
            <p>O<sub>2</sub>+ RFP ---7,8,9,10,11,12,13 -890</p>
 
-
            <p>Fe+RFP ---14,15,16,17,18,19 -1,787</p>
 
-
            <br />
 
-
            <p>GFP -&gt; 761 bp EcoRI Po+I // 728 bp XbaI SpeI</p>
 
-
            <p>RFP -&gt; 714 bp Xba - SpeI // 747 bp EcoR - Ps+I</p>
 
-
            <br />
 
-
            <p>pFe -&gt; 1,067 bp EcoRI - SpeI </p>
 
-
            <p><sub>p</sub>O<sub>2</sub> -&gt; 178 bp EcoRi SpeI // 145 bp Xba -
 
-
              Ps+I </p>
 
-
          </div>
 
-
          <div>
 
-
            <p>O<sub>2</sub> + GFP 1/1 - 1/2 - 3/3</p>
 
-
            <p>FO <sub>2</sub> + GFP 4/1 - 5/2 - 6/3</p>
 
-
            <p>O<sub>2</sub> + RFP 7/1 - 8/2 - 9/3 - 10/4 - 11/5 - 12/6 - 12/13
 
-
            </p>
 
-
            <p>Fe + RFP 13/1 - 14/2 - 15*16/3 - 17/4 - 18/5 -19/6</p>
 
-
            <h1>Digestion</h1>
 
-
            <p>2 --- BSA --- 1uL</p>
 
-
            <p>4 --- DNA --- 2uL</p>
 
-
            <p>3 --- (M) Buffer // Enz (0.3 uL each one)--- 1uL</p>
 
-
            <p>5 --- EcoRI --- 0.3 uL</p>
 
-
            <p> --- PstI --- 0.3 uL</p>
 
-
            <p>1 --- H<sub>2</sub>O --- 5.4 uL</p>
 
-
            <p> Total == 10 uL rxn</p>
 
-
            <br />
 
-
            <p> GFP 761 bp</p>
 
-
            <p> RFP 742 bp</p>
 
-
          </div>
 
-
          <div>
 
-
            <p> pFe 1,067 bp </p>
 
-
            <p> pO<sub>2</sub> -15.5 bp</p>
 
-
            <br />
 
-
            <p> pO<sub>2</sub> + GFP 931 bp</p>
 
-
            <p> pFe + GFP 1,828 bp</p>
 
-
            <p> pO<sub>2</sub>+ RFP 890 bp</p>
 
-
            <p> pFe + RFP 1,787 bp</p>
 
-
            <br />
 
-
            <p> Gel 1 = 8/9/10/Lodder 1kb/11/12/13</p>
 
-
            <p> Gel 2 = 15/16/17/Lodder 116/18/19</p>
 
-
            <p> Gel 3 = 1/2/3/4/Lodder 1kb/5/6/7</p>
 
-
          </div>
 
-
        </div>
 
-
      </div>
 
-
    </div>
 
-
    <div>  <span class="reference"> </span> </div>
 
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</body>
 
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</html>
 

Latest revision as of 03:20, 21 June 2014