2014hs.igem.org/team:Charlottesville RS/Notebook/041514

From 2014hs.igem.org

(Difference between revisions)
(Created page with "__NOTOC__ <html> <style> h1.firstHeading { display: none; } p {text-align: justify;} a:link { color: #00b0e6; text-decoration: none} a:visited { color:#00b0e6; text-decorati...")
(→April 15th, 2014)
Line 403: Line 403:
==April 15th, 2014==
==April 15th, 2014==
 +
 +
''Nick Keen, Anders Beaurline''
 +
 +
Took cells off of plate that was incubating overnight. Suspended them in calcium chloride and put them on ice for 15 minutes. Switched from Carolina procedure to our own starting at step 6 of the “producing competent cells” sheet, then centrifuged and resuspended cells in cold calcium chloride. We are hoping that the cells are competent.
 +
 +
 +
----
 +
 +
 +
''Anders Beaurline''
 +
 +
Mixed competent cells with DNA of concentrations (in pg/uL): 50, 25, 10, 5, .5. Incubated on ice for 30 minutes, then heat shocked, then put back on ice.
 +
 +
 +
----
 +
 +
 +
''Becky Wilbur''
 +
 +
Plated transformed (we hope) cells as in step number 7 on page 29 in the iGem procedure notebook and put in 37 degrees (Celsius) incubator.

Revision as of 13:27, 28 April 2014

April 15th, 2014

Nick Keen, Anders Beaurline

Took cells off of plate that was incubating overnight. Suspended them in calcium chloride and put them on ice for 15 minutes. Switched from Carolina procedure to our own starting at step 6 of the “producing competent cells” sheet, then centrifuged and resuspended cells in cold calcium chloride. We are hoping that the cells are competent.



Anders Beaurline

Mixed competent cells with DNA of concentrations (in pg/uL): 50, 25, 10, 5, .5. Incubated on ice for 30 minutes, then heat shocked, then put back on ice.



Becky Wilbur

Plated transformed (we hope) cells as in step number 7 on page 29 in the iGem procedure notebook and put in 37 degrees (Celsius) incubator.

Retrieved from "http://2014hs.igem.org/2014hs.igem.org/team:Charlottesville_RS/Notebook/041514"