2014hs.igem.org/team:Charlottesville RS/Notebook/041114

From 2014hs.igem.org

(Difference between revisions)
(April 11th, 2014)
(April 11th, 2014)
Line 413: Line 413:
''Alli Ambrosini, Lauren Ewell''
''Alli Ambrosini, Lauren Ewell''
-
We did the transformation efficiency kit sent by iGEM, to see how well out competent cells could transform the DNA. We used 5 different concentrations of DNA- .5mL, 5mL, 10mL, 25mL, and 50mL. We definitely should see at least some red cells in the 50mL plates, and possibly none in our .5mL plates.  
+
Performed the transformation efficiencies kit sent by iGEM, to see how well out competent cells could transform the DNA. Five different concentrations of DNA- .5uL, 5uL, 10uL, 25uL, and 50uL were used. Expecting to see at least some red cells in the 50uL plates, and possibly none in our .5uL plates.  

Revision as of 13:18, 5 May 2014

April 11th, 2014

John Grammer

Transformation with DNA generated with ligation on 3-28-14. Followed protocol on pages 19-20 to step #10




Alli Ambrosini, Lauren Ewell

Performed the transformation efficiencies kit sent by iGEM, to see how well out competent cells could transform the DNA. Five different concentrations of DNA- .5uL, 5uL, 10uL, 25uL, and 50uL were used. Expecting to see at least some red cells in the 50uL plates, and possibly none in our .5uL plates.




Becky Wilbur

Plated transformed cells after their 2 hours in the incubator. Followed steps 11 and 12 on page 20.




Becky Wilbur

Plated transformed cells from transformation efficiency kit. Followed step #7 on page 29.

[Back]