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2014hs.igem.org/team:Charlottesville RS/Notebook/041114
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Transformation with DNA generated with ligation on 3-28-14. Followed protocol on pages 19-20 to step #10 | Transformation with DNA generated with ligation on 3-28-14. Followed protocol on pages 19-20 to step #10 | ||
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We did the transformation efficiency kit sent by iGEM, to see how well out competent cells could transform the DNA. We used 5 different concentrations of DNA- .5mL, 5mL, 10mL, 25mL, and 50mL. We definitely should see at least some red cells in the 50mL plates, and possibly none in our .5mL plates. | We did the transformation efficiency kit sent by iGEM, to see how well out competent cells could transform the DNA. We used 5 different concentrations of DNA- .5mL, 5mL, 10mL, 25mL, and 50mL. We definitely should see at least some red cells in the 50mL plates, and possibly none in our .5mL plates. | ||
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Plated transformed cells after their 2 hours in the incubator. Followed steps 11 and 12 on page 20. | Plated transformed cells after their 2 hours in the incubator. Followed steps 11 and 12 on page 20. | ||
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Revision as of 13:24, 28 April 2014
April 11th, 2014
John Grammer
Transformation with DNA generated with ligation on 3-28-14. Followed protocol on pages 19-20 to step #10
Alli Ambrosini, Lauren Ewell
We did the transformation efficiency kit sent by iGEM, to see how well out competent cells could transform the DNA. We used 5 different concentrations of DNA- .5mL, 5mL, 10mL, 25mL, and 50mL. We definitely should see at least some red cells in the 50mL plates, and possibly none in our .5mL plates.
Becky Wilbur
Plated transformed cells after their 2 hours in the incubator. Followed steps 11 and 12 on page 20.
Becky Wilbur
Plated transformed cells from transformation efficiency kit. Followed step #7 on page 29.
