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2014hs.igem.org/team:Charlottesville RS/Notebook/041114
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''Alli Ambrosini, Lauren Ewell'' | ''Alli Ambrosini, Lauren Ewell'' | ||
| - | We did the transformation | + | We did the transformation efficiency kit sent by iGEM, to see how well out competent cells could transform the DNA. We used 5 different concentrations of DNA- .5mL, 5mL, 10mL, 25mL, and 50mL. We definitely should see at least some red cells in the 50mL plates, and possibly none in our .5mL plates. |
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''Becky Wilbur'' | ''Becky Wilbur'' | ||
| - | Plated transformed cells from transformation efficiency kit. | + | Plated transformed cells from transformation efficiency kit. Followed step #7 on page 29. |
Revision as of 16:43, 26 April 2014
April 11th, 2014
John Grammer
Transformation with DNA generated with ligation on 3-28-14. Followed protocol on pages 19-20 to step #10
Alli Ambrosini, Lauren Ewell
We did the transformation efficiency kit sent by iGEM, to see how well out competent cells could transform the DNA. We used 5 different concentrations of DNA- .5mL, 5mL, 10mL, 25mL, and 50mL. We definitely should see at least some red cells in the 50mL plates, and possibly none in our .5mL plates.
Becky Wilbur
Plated transformed cells after their 2 hours in the incubator. Followed steps 11 and 12 on page 20.
Becky Wilbur
Plated transformed cells from transformation efficiency kit. Followed step #7 on page 29.
