2014hs.igem.org/team:Charlottesville RS/Notebook/041114

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(March 11th, 2014)
 
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<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Project"><span><span>Overall Project</span></span></a></li>
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<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Notebook/material"><span><span>Material & Methods</span></span></a>
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<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Notebook/material"><span><span>Material & Methods</span></span></a></li>
<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Project/Applications"><span><span>Applications</span></span></a></li>
<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Project/Applications"><span><span>Applications</span></span></a></li>
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<a href="https://2014hs.igem.org/Team:Charlottesville_RS/Parts" style="color: white">Parts<!--[if gt IE 6]><!--></a><!--<![endif]-->
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<li><a href="https://2014hs.igem.org/Team:Charlottesville_RS/Parts"><span><span>Submitted Parts</span></span></a></li>
 
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<a href="https://2014hs.igem.org/Team:Charlottesville_RS/Notebook/Overview" style="color: white">Notebook<!--[if gt IE 6]><!--></a><!--<![endif]-->
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Transformation with DNA generated with ligation on 3-28-14. Followed protocol on pages 19-20 to step #10
Transformation with DNA generated with ligation on 3-28-14. Followed protocol on pages 19-20 to step #10
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''Alli Ambrosini, Lauren Ewell''
''Alli Ambrosini, Lauren Ewell''
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We did the transformation efficiencies kit sent by iGem, to see how well out competent cells could transform the DNA. We used 5 different concentrations of DNA- .5mL, 5mL, 10mL, 25mL, and 50mL. We definitely should see at least some red cells in the 50mL plates, and possibly none in our .5mL plates.  
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Performed the transformation efficiencies kit sent by iGEM, to see how well out competent cells could transform the DNA. Five different concentrations of DNA- .5uL, 5uL, 10uL, 25uL, and 50uL were used. Expecting to see at least some red cells in the 50uL plates, and possibly none in our .5uL plates.  
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Plated transformed cells after their 2 hours in the incubator. Followed steps 11 and 12 on page 20.
Plated transformed cells after their 2 hours in the incubator. Followed steps 11 and 12 on page 20.
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''Becky Wilbur''
''Becky Wilbur''
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Plated transformed cells from transformation efficiency kit. Fellowed step #7 on page 29.
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Plated transformed cells from transformation efficiency kit. Followed step #7 on page 29.
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[[https://2014hs.igem.org/Team:Charlottesville_RS/Notebook/Overview Back]]

Latest revision as of 13:59, 20 June 2014

April 11th, 2014

John Grammer

Transformation with DNA generated with ligation on 3-28-14. Followed protocol on pages 19-20 to step #10




Alli Ambrosini, Lauren Ewell

Performed the transformation efficiencies kit sent by iGEM, to see how well out competent cells could transform the DNA. Five different concentrations of DNA- .5uL, 5uL, 10uL, 25uL, and 50uL were used. Expecting to see at least some red cells in the 50uL plates, and possibly none in our .5uL plates.




Becky Wilbur

Plated transformed cells after their 2 hours in the incubator. Followed steps 11 and 12 on page 20.




Becky Wilbur

Plated transformed cells from transformation efficiency kit. Followed step #7 on page 29.

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