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2014hs.igem.org/team:Charlottesville RS/Notebook/041114
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| - | == | + | == April 11th, 2014 == |
| + | ''John Grammer'' | ||
| + | |||
| + | Transformation with DNA generated with ligation on 3-28-14. Followed protocol on pages 19-20 to step #10 | ||
| + | |||
| + | |||
| + | ---- | ||
| + | |||
| + | |||
| + | ''Alli Ambrosini, Lauren Ewell'' | ||
| + | |||
| + | Performed the transformation efficiencies kit sent by iGEM, to see how well out competent cells could transform the DNA. Five different concentrations of DNA- .5uL, 5uL, 10uL, 25uL, and 50uL were used. Expecting to see at least some red cells in the 50uL plates, and possibly none in our .5uL plates. | ||
| + | |||
| + | |||
| + | ---- | ||
| + | |||
| + | |||
| + | ''Becky Wilbur'' | ||
| + | |||
| + | Plated transformed cells after their 2 hours in the incubator. Followed steps 11 and 12 on page 20. | ||
| + | |||
| + | |||
| + | ---- | ||
| + | |||
| + | |||
| + | ''Becky Wilbur'' | ||
| + | |||
| + | Plated transformed cells from transformation efficiency kit. Followed step #7 on page 29. | ||
| + | |||
| + | [[https://2014hs.igem.org/Team:Charlottesville_RS/Notebook/Overview Back]] | ||
Latest revision as of 13:59, 20 June 2014
April 11th, 2014
John Grammer
Transformation with DNA generated with ligation on 3-28-14. Followed protocol on pages 19-20 to step #10
Alli Ambrosini, Lauren Ewell
Performed the transformation efficiencies kit sent by iGEM, to see how well out competent cells could transform the DNA. Five different concentrations of DNA- .5uL, 5uL, 10uL, 25uL, and 50uL were used. Expecting to see at least some red cells in the 50uL plates, and possibly none in our .5uL plates.
Becky Wilbur
Plated transformed cells after their 2 hours in the incubator. Followed steps 11 and 12 on page 20.
Becky Wilbur
Plated transformed cells from transformation efficiency kit. Followed step #7 on page 29.
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