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Resources
From 2014hs.igem.org
Below are some resources that may come in handy as you're working on your projects for iGEM 2014 High School!
Wiki editing
- MediaWiki has detailed instructions for wiki editing, including the following:
Purchasing laboratory reagents and materials
- Fisher Scientific offers a wide array of common laboratory materials and reagents
- Sigma Aldrich offers many chemical compounds, including antibiotics
Databases and websites to explain concepts:
- Parts Registry Help page is where you can find information about BioBrick Parts and how to use them
- Team Virginia 2013 developed a high school education series including slides, lab exercises, and readings
- BioBuilder has a series of synthetic biology laboratory exercises
- OpenWetWare is a source for protocols and other lab-related information
- CompuBioTicDB is a database of biological components for synthetic biology
Tutorials:
- Polymerase chain reaction (PCR) (University of Utah)
- Primer design and ordering (OpenWetWare)
- Ligation and plasmid construction (BioBuilder)
Software tools:
- FAQ section on iGEM.org answers questions about wiki-editing
- TinkerCell is a free "Computer-Aided Design software tool for Synthetic Biology"
- GENtle is free software that allows users to edit DNA sequences, generate plasmid maps, and more
Protocols:
- Making competent cells (Registry)
- Competent cell transformation (Registry)
- Ligation (Registry)
- Restriction digest (Registry)
- Ligation and Transformation module (Bio-Rad)
- Yeast transformation (University of Manitoba)
